LOCUS       NZ_AP022344             6258 bp    DNA     circular CON 14-FEB-2024
DEFINITION  Enterococcus faecium strain KUHS13 plasmid pKO3, complete sequence.
ACCESSION   NZ_AP022344
VERSION     NZ_AP022344.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00202474
            Sequence Read Archive: DRR205461, DRR205462
            Assembly: GCF_009938285.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Hashimoto,Y., Kita,I., Suzuki,M., Ohtaki,H. and Tomita,H.
  TITLE     The single hospital outbreak of multispecies of vancomycin-resistant
            enterococci in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6258)
  AUTHORS   Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2020) Contact:Yusuke Hashimoto Gunma university
            graduate school of medicine, bacteriology; 3-39-22 Showa-machi,
            Maebashi, Gunma 3718511, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Coverage                   :: 100x
            Sequencing Technology :: Nanopore MinION; Illumina MiniSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/14/2024 01:38:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,160
            CDSs (total)                      :: 3,069
            Genes (coding)                    :: 2,841
            CDSs (with protein)               :: 2,841
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 228
            CDSs (without protein)            :: 228
            Pseudo Genes (ambiguous residues) :: 0 of 228
            Pseudo Genes (frameshifted)       :: 142 of 228
            Pseudo Genes (incomplete)         :: 93 of 228
            Pseudo Genes (internal stop)      :: 34 of 228
            Pseudo Genes (multiple problems)  :: 32 of 228
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022344.1.
            Annotated at DFAST https://dfast.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="KUHS13"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pKO3"
     gene            1..951
                     /locus_tag="EfmKUHS13_RS15550"
                     /old_locus_tag="EfmKUHS13_31550"
     CDS             1..951
                     /locus_tag="EfmKUHS13_RS15550"
                     /old_locus_tag="EfmKUHS13_31550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_033642128.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRL
                     TFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIRGT
                     AKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVVGYQF
                     TFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQI
                     KQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            972..1505
                     /locus_tag="EfmKUHS13_RS15555"
                     /old_locus_tag="EfmKUHS13_31560"
     CDS             972..1505
                     /locus_tag="EfmKUHS13_RS15555"
                     /old_locus_tag="EfmKUHS13_31560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002321384.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLREQL
                     ALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATETID
                     KQIDTEKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            1521..1691
                     /locus_tag="EfmKUHS13_RS15710"
                     /old_locus_tag="EfmKUHS13_31570"
     CDS             1521..1691
                     /locus_tag="EfmKUHS13_RS15710"
                     /old_locus_tag="EfmKUHS13_31570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            2141..2374
                     /locus_tag="EfmKUHS13_RS16020"
                     /old_locus_tag="EfmKUHS13_31580"
     CDS             2141..2374
                     /locus_tag="EfmKUHS13_RS16020"
                     /old_locus_tag="EfmKUHS13_31580"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_232067670.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENVV
                     IEYTEDGMVVTKYEDGYSELQDGAKRNIQILL"
                     /name="hypothetical protein"
     gene            2440..2631
                     /locus_tag="EfmKUHS13_RS16025"
                     /old_locus_tag="EfmKUHS13_31590"
     CDS             2440..2631
                     /locus_tag="EfmKUHS13_RS16025"
                     /old_locus_tag="EfmKUHS13_31590"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_232067671.1"
                     /translation="MFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYY
                     QRGYDAAGRVVATRYSTL"
                     /name="hypothetical protein"
     gene            2643..2846
                     /locus_tag="EfmKUHS13_RS15565"
                     /old_locus_tag="EfmKUHS13_31600"
     CDS             2643..2846
                     /locus_tag="EfmKUHS13_RS15565"
                     /old_locus_tag="EfmKUHS13_31600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002321382.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQL
                     YKSGLFLVLILVVLDIIKSKKK"
                     /name="hypothetical protein"
     gene            3166..3546
                     /locus_tag="EfmKUHS13_RS15570"
                     /old_locus_tag="EfmKUHS13_31610"
     CDS             3166..3546
                     /locus_tag="EfmKUHS13_RS15570"
                     /old_locus_tag="EfmKUHS13_31610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_002321381.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPDDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3528..4442
                     /locus_tag="EfmKUHS13_RS15575"
                     /old_locus_tag="EfmKUHS13_31620"
     CDS             3528..4442
                     /locus_tag="EfmKUHS13_RS15575"
                     /old_locus_tag="EfmKUHS13_31620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002321380.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRT
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKKKVRS
                     NKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTPEQSEI
                     RPTRRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4402..4680
                     /locus_tag="EfmKUHS13_RS15580"
                     /old_locus_tag="EfmKUHS13_31630"
     CDS             4402..4680
                     /locus_tag="EfmKUHS13_RS15580"
                     /old_locus_tag="EfmKUHS13_31630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058138385.1"
                     /translation="MNEEQTTNKNYRSKTLKDIENEIKRQRAFLAPYTQERIQTTMDSQ
                     VKKLHQALLDQRNQEQKKALIQRSMDILAPLCCLVLTLLAFWLVWGE"
                     /name="hypothetical protein"
     gene            4687..5376
                     /locus_tag="EfmKUHS13_RS15585"
                     /old_locus_tag="EfmKUHS13_31640"
     CDS             4687..5376
                     /locus_tag="EfmKUHS13_RS15585"
                     /old_locus_tag="EfmKUHS13_31640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002321377.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLVG
                     VIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTACY
                     EDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQTEEA
                     CCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLESVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     Typing          3..951
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.953"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3529..4439
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.079"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttggcaaatg aaatcgtgaa ataccatcat gaattaaata ccattccgtt gcggaaattt
       61 acaccagttg aaatgaatct atttttctct attgtgtcta gaatgcgcga aaaaggcgat
      121 aaaacagtcc gtcttacttt tgaccaacta aaagatttaa gtaattataa agcaactgct
      181 aataatcgtt tcattaatga tttagaaatg acttatgata aattaatgga tttacgtttt
      241 ggtaaaagaa gtgctaatgg gctcgatata gatagattcg ttatgtttag tgagtttaaa
      301 atacgtggaa ctgctaaaga tccatatgta gacatacaag tttacgaaaa agctttacct
      361 ttactaaaca atttagatga gtgggttcgc tattcactcc aacaatttaa cgaattacaa
      421 agtagctatg caaagactat gtttcgatta ctaaaacaat atcgaactaa aggttttgct
      481 tatttttcta aagaggattt tcatgaacta ttagatattc caaaaagtta ctggaacaac
      541 caagcaaacg ttgataatcg tgttataaag cctatacgac aggaattaac cgcaatcttc
      601 aaaggattat caatcaaaaa gaaatacgga aaagggcgag gaaaacctgt cgttggctat
      661 caatttacgt ttaaaccaga aatcaaaaaa gcagatgatt ttaataaaca tgcaccaacc
      721 aaagaagaac aaaataaagc attggcttta gctgataaac aaaaagaata cgaagaacct
      781 gctacaacaa ttaacgaaaa aatcaaacag aagaaacgag aacttgaact cctccaacaa
      841 atcaaacaac tagaaaaaga gaaagaagct cttgaaaaac aaacagagtc cgcactttcg
      901 aaaaaagaga aaaaaagttt attatctgat ttagataatt tattccagta gcaacctagg
      961 aggcaaccag tatgcaacca caagaaacct ttacaataaa ggaattgtca gaacttttta
     1021 agatgagtag gcaagcaata tcgaaacata tccaaaaatt agattcttcc atgatagcta
     1081 aaaatgagcg tggttataag gttgtattgc gttctggagt attgcaactt gctcgcaact
     1141 taggcaacca aatgttgcta gataggttgc acgagtacga acaagacaac gataaaaatg
     1201 ttaatatacc aagcaaaaac gtggttacaa acaagttgct agatcagttg cgtgaagaaa
     1261 atgaattttt acgagaacaa cttgccctca gagagcaaaa taattctcat atacaaaaac
     1321 tactcgacca gcaacaacaa ctcaccctac aagccaacaa acaaatcgaa aaactgcaag
     1381 aaaaactaca gttgacttat acagaagaag ctccagagaa tgactcaacg actctttctg
     1441 aagcaacaga aacaatagac aaacaaattg atactgaaaa aaaatggtgg caactttgga
     1501 aataactgga gggaacattc atgacaagaa aaaatcttct attcggttct attttactag
     1561 gaattctagg attcagtccg atgttattgt cagtgatcaa taaagggaat aaacatggtg
     1621 aattgaaatt gaatccaaat tattacaaca gtcctaacga agaattgttt gtaaaagaaa
     1681 cagataaata ataaaaaaga ctgctctttc gagcagccag ttaaaaaata atgagtgttt
     1741 attcttgaaa aagcaacgcg cttatgaagc gctaccaaaa atttatcata ttttattaaa
     1801 aataacaacc cagtaaaaaa tgactggtaa aaattcacaa agaaacggaa agaagcaacc
     1861 gcagaaaatc aagcttaaaa agagcttttg acctagacca atcgaaaatc acaaagtgag
     1921 cctctgtatc ggttgataca agggctactt ggcatagtcg acattatgtc aacaagaatt
     1981 taatataaca tattttccta atgcattcta actataccac attatcaaaa atataaatta
     2041 tctttactaa ataaaatatt gacaagccta taccttttgt tttatgattt tcacaaagaa
     2101 aacgttttct atatattttt ctgtatcagg aggaaattct atgaaaaaat ttactttagg
     2161 ggtagctttg ttaactattg gggcagttgt tttaccgtca actactatag tttatgcttc
     2221 agaaaattcc caagtggtat cattaaacga aaacaatgct actgaaaatg tagttattga
     2281 gtacactgaa gatggtatgg tcgtaactaa atatgaggat ggttatagtg agttgcaaga
     2341 tggggcaaaa aggaatatac aaatattgct gtaaccactg gagtagctgc taatgctatt
     2401 aatgctgcct tttgggcagg agggaaagct agcacttcta tgtttggtat cccaggttgg
     2461 gcaattggtg ctttgttaac aggtgcaagt tggactaaaa aaggtgcttc tccagggaat
     2521 gcagttgcaa aagaatggga caaaaatggt aatggctgga ttggatttta ttaccaaagg
     2581 ggatatgatg cagctggaag agttgtagct actagatatt caacgttata agttaaggag
     2641 tgatgttttt gatatctaaa tcattaaaat cattaaaaac atttactaaa aagaatccgc
     2701 aaatagctac tattcgtatt tttatatttg ctatagctgt tgctataatt gttaatatgc
     2761 taagcggagg gcaattatac aaatctggtt tatttttagt gttaatctta gttgtcttag
     2821 acataataaa gtctaaaaaa aaataaatcg atacatttta gaagaatagg gttgtttgaa
     2881 tattacaaag caacaatttc accttcggtg aaataagcga aactttacaa aaagtggcta
     2941 gcaaatgcta gcctttttgt attgtggagc gtggattttg agggggattc aagggggagc
     3001 ttgcgctcct ttgacaagcg gttatttgca ccaaatacgg aggtatttgg ctagcaaata
     3061 tagagcttgc caaacccttt tagtgagctt tactcattgt acctttttag gtacaatggt
     3121 attgcgaacg atacgtccgt aggacgtact gaaagggtgg gacttatgag cgaacaacat
     3181 caaaatttgg ctagcgatca ttctaaaaaa tatacctatc gatccgagcc aaaacaaatc
     3241 agttttcgag tgagcgaatc cgaatttgca aagctgaagc agtcagctga agctttgcaa
     3301 atgagtgtgc cggctttcgt gaaagccaag gcacaaaacg cgcgtctggt gacaccgaaa
     3361 gtggcgccag acattgccca ggcaatcgcg cgcgatttag ccaaagctgg agggaatatc
     3421 aatcaaattg ccaagtggtg caatacacac caacacgacg ttgctcctga tgatgctcaa
     3481 cgtttatccg aaaacctaaa aatcatgcaa aaggagctac aaaagatatg gcaacagtta
     3541 aagtaagtcg aaccacttca tgtagccgag caatcaatta cgcagaacca cgagcaaccg
     3601 tcaaaacagg aataaactgt gacattgatt atgccaaaag tgaaatgaaa caaatacgca
     3661 cgctttatgg aaaagacgat cgtgtacaag cacacttatt gattcagtcc tttcgaccag
     3721 gagaaattac cgctgaaaaa gccaatcaac taggggcaga gtatgcgaaa aaaatagccc
     3781 ctaaccacca aatcgcgatt tacacacata cagacaaaga ccatattcat aatcacattg
     3841 tcattaactc cgttaatctt gaaactggga aaaaatttca agcccatggg caagcatttt
     3901 taaacaaatg ttatgaccta aatgacgaga tttgtcttga acatgactta acaatcacgg
     3961 aacgaggaaa gaaaaccgaa aaacgaacca tgtctgaaat taaattaaaa gaaaaaaatg
     4021 aaccggtttg gaaagacgaa attcggtttg cgattgatca aacaatgaaa aacaaaaaaa
     4081 ctcgaacgta tacccaattt tgtgattcac ttaaaacatt cggcattcat tgcttgaatc
     4141 gcggaaaaaa ttttacttac gaattggtcc acaaaaagaa aaaagtacga tccaacaaac
     4201 taggaaagga ttacgaaaag gagacgattt tacatgagct ggacagacga gaacgaacct
     4261 acaatcaaca acgaaccaac gagctacgaa gagctcgagc tagcattcgc gaaactgcag
     4321 aagaagtacg aagagaacaa cgaactttta cgcctgaaca atcggaaatt agaccgactc
     4381 gaagaaacca tgaacaacac gttgaacgag gaacagacca ccaacaagaa ttatcgctct
     4441 aaaacgttaa aagacatcga aaacgaaata aagagacaac gtgcctttct tgcgccttat
     4501 acccaagaaa ggatacaaac tacgatggat tcacaagtca aaaaactcca ccaagcgcta
     4561 ttagaccagc gaaaccaaga acaaaaaaag gctttaattc aacgatcgat ggatattctt
     4621 gcccccttat gttgcttagt actaacatta ttagcctttt ggttggtatg gggagaataa
     4681 acagacatgg cattctattt accctatcta ctcatttctg ccagtatttt aggaattatt
     4741 tggcttagct ataaggctta tcaaacaaga tattcattaa aaggctctaa aattcgattt
     4801 aagcgatttt tattggtcgg ggttatattt gccttatcaa tcgttttaag tggtttattc
     4861 ggcgttttag agggtaataa acgtgtttca agaagcattt tattagggga tatcactcaa
     4921 aagtatgaag cagcatgtga taaaaagaaa aaagagcaag cattagcaca aaaaatggag
     4981 gaatttaccg cttgttatga ggacatgaac aatatttttg tggatcaaga gaaacgttta
     5041 acagataaaa acatgaaaaa actcactcgt ttttatcaaa acttaccaga aaaacagcaa
     5101 aaagaggtac aagaaaaata tgagcaagta aaaaaagacg tgcaatattt taaagatacg
     5161 cagacagaag aagcttgttg taacttattt agcgatacaa tccctttttc aaccagtgaa
     5221 caagaacgaa aagaacggca acaatcggtc acatacgaac gttatgaaac actgcttcag
     5281 caagcaacaa acatacaaaa tccaacgaaa aaagaaacgg cattaaatta tctagaaagt
     5341 gtcaaggaat ggttggatca gcaacaacaa aattgaattt gattagcaat catgttaaac
     5401 tgaacctgca aatgatcaaa cattagatac acgaaagccc cagagaggtc ggattctctg
     5461 gggctttttt tcaggttgat gtcgccttta ttgattttac ttcttgtttc ggttattcaa
     5521 ccaataactg aataaagaga cacaaacttc tacaagaagg gggcaaatga accgttcaaa
     5581 cattagatac acctccttcc agtcgcaaga ctgtcggata ggcgacaagt tattatacct
     5641 tacaaaataa aatctgtgtg gtgtttttaa gtatccctta ttttcgggtt gttagggctt
     5701 gccctgaccg cctgtaaggc gctttaagaa caagataagg ttttttaccc aagactgtct
     5761 taaaacgact tagacgagct atcagaggct tcccccacct ccgttactga cttgttttta
     5821 gcaaaggagc ctttaggcga ctgcgcagta caatgtgaga gcatagcgat cacacttttt
     5881 tagcgaccgt agggagcaag gttttgaatt ggcagttttg aaaaatgcgc gaacctgttg
     5941 gtagaaaaaa gaagtggttt ttaggcggga aattttgtca ccctctttta taatctcttt
     6001 ttaaaccttt ttttaaacct ctttaccgtc ggctgtgtcc ttactccttc aatggataca
     6061 gcgttttata aactacattt tgtctgcttt taaactacat tttgtctgct tttaaactac
     6121 attttgtctg cttttaaact acattttgtc tgcttttaaa ctcttttttt gcactcacgt
     6181 atgttataat atgcattatc acagacaaaa cgttgtttat aacgtctgta tccattgaag
     6241 gagtaaggag gaatatct
//
