LOCUS       NZ_AP022304            11158 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Acinetobacter sp. BEC1-S18-ESBL-01 plasmid pBEC1-S18-ESBL-01_2,
            complete sequence.
ACCESSION   NZ_AP022304
VERSION     NZ_AP022304.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194714
            Sequence Read Archive: DRR199550
            Assembly: GCF_014169935.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter sp. BEC1-S18-ESBL-01
  ORGANISM  Acinetobacter sp. BEC1-S18-ESBL-01
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Acinetobacter sp. BEC1-S18-ESBL-01
            isolated from oceanic water
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11158)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 302x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 08:46:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,861
            CDSs (total)                      :: 3,766
            Genes (coding)                    :: 3,678
            CDSs (with protein)               :: 3,678
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 37 of 88
            Pseudo Genes (incomplete)         :: 47 of 88
            Pseudo Genes (internal stop)      :: 19 of 88
            Pseudo Genes (multiple problems)  :: 11 of 88
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022304.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11158
                     /organism="Acinetobacter sp. BEC1-S18-ESBL-01"
                     /mol_type="genomic DNA"
                     /strain="BEC1-S18-ESBL-01"
                     /isolation_source="sea water"
                     /db_xref="taxon:2675741"
                     /plasmid="pBEC1-S18-ESBL-01_2"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6306 N 139.7754 E"
                     /collection_date="2018-08-06"
     gene            101..1030
                     /gene="repM"
                     /locus_tag="H7R46_RS19145"
                     /old_locus_tag="BE1S18E01_P20010"
     CDS             101..1030
                     /gene="repM"
                     /locus_tag="H7R46_RS19145"
                     /old_locus_tag="BE1S18E01_P20010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065049.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_005065049.1"
                     /translation="MSELIVKDNALIQASYTLDTVEQRLILLAIAEARETGHGITENSL
                     LEVHASSYINTFNVEKHTAYTVLRDASKSLFDRYVTYHDINPKTGKDRSFHCRWVDKIG
                     YEPQSGIVFLRFTQDIVPLITRLEENFTKYELEQVSRLTSSYAIRLYELLIQWRSAGKT
                     PIFDLSIFRQQLGVEAHQYKTMSNFKTYVLDFALKQVNELTDITAKYEQHKKGRSISGF
                     SFSFKQKKTNSDKVIKGTDTLALFTKMSDKQRHLFANKLSELPEMGQYSEGTESFQQFA
                     IRIAEMLQDYQKFQELFPYLQKVGFAPA"
                     /name="repM"
     gene            1109..1639
                     /locus_tag="H7R46_RS19150"
                     /old_locus_tag="BE1S18E01_P20020"
     CDS             1109..1639
                     /locus_tag="H7R46_RS19150"
                     /old_locus_tag="BE1S18E01_P20020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017397202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_005065050.1"
                     /translation="MKSLTVLELSKLYNINRQTIYNNIKKGILSKNSQNKIDLAEAIRV
                     FGEPVKKQDVKEPVKIDSPNSAEVLLLRQQIDMLKNQLDDAKDRESFYQNQIETMQRLL
                     EAPKPNMTTFTDQELVQPSIAKPDPEPDTAEAKHDGLTTPERRENKWIPVPEHIEPEPE
                     KRGFWSRFFRPYD"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(1871..2722)
                     /locus_tag="H7R46_RS19155"
                     /old_locus_tag="BE1S18E01_P20030"
     CDS             complement(1871..2722)
                     /locus_tag="H7R46_RS19155"
                     /old_locus_tag="BE1S18E01_P20030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034565.1"
                     /GO_function="GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="WP_005065068.1"
                     /translation="MSKIIACIDGSLATNTVCDYAAWFSDKLSSPLKLLHVIDKPKAKA
                     PQDLSGAIGLGSRETLLKELVEIEERKGKIELEHGQILLREAKNYLLEKFSIDAQSFQR
                     HGSLLETIMGMEEDIRVLVMGKHGAETEHDSSKVGTHIENVVRALHKPVLITSSPFSPP
                     KSFLIAFDGSPTARICVDKIANSPLLKTLTAHVVYVGKPNSDMTLQVTWAKELLANNGF
                     DVIDSMLDGDVEKSILDYQEKNVIDMIVIGAYGHSKIRQFFIGSTTTKLLSSSTKPVLL
                     LR"
                     /name="universal stress protein"
     gene            complement(2735..4222)
                     /locus_tag="H7R46_RS19160"
                     /old_locus_tag="BE1S18E01_P20040"
     CDS             complement(2735..4222)
                     /locus_tag="H7R46_RS19160"
                     /old_locus_tag="BE1S18E01_P20040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953322.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="WP_005065066.1"
                     /translation="MLSTIREQWFSNIRGDVLAGIVVALALIPEAIAFSIIAGVDPKVG
                     LYASFCIAVVIAFVGGRPGMISGATGAMALLMVTLVKEHGLQYLLAATILTGFIQILAG
                     YLKLGALMRFVSKSVVIGFVNALAILIFMAQLPELTNVTWHVYAMTIGGLAIIYLFPYI
                     PVIGKLLPSPLICIVLLTLFALFIGLDVRTVGDMGQLPDTLPIFLLPDIPLNLETLTII
                     LPYSLGLAAVGLLESMMTATIVDDLTDTNSDKNRECKGQGVANIASGFLGGMAGCAMIG
                     QSIINVKSGGGTRLSTFSAGVFLLIMVVFISDWLKAIPMAALVAVMIMVAIGTFNWDSL
                     RNIRQYPLSSNIVMIVTVIVVVATHNLAYGVLVGVLLSALFFANKIERYMAIQSEFNEP
                     ENTRTYTVTGQVFFSSADKFTSAFDFKEALSKVVIDVSQAHFWDVSAVAAVDKVVIKFR
                     REGTEVELIGMNEASKTLVDKFGIHDKPDALDRLNSH"
                     /name="SulP family inorganic anion transporter"
     gene            4735..5022
                     /locus_tag="H7R46_RS19165"
                     /old_locus_tag="BE1S18E01_P20050"
     CDS             4735..5022
                     /locus_tag="H7R46_RS19165"
                     /old_locus_tag="BE1S18E01_P20050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_002124950.1"
                     /translation="MELYFEWDEAKNQKNQKKHDVSFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGMVKGVLMLLVAHTIIDDDDCEIIRIISARQVTKAERNQYEHG"
                     /name="BrnT family toxin"
     gene            5009..5320
                     /locus_tag="H7R46_RS19170"
                     /old_locus_tag="BE1S18E01_P20060"
     CDS             5009..5320
                     /locus_tag="H7R46_RS19170"
                     /old_locus_tag="BE1S18E01_P20060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_086260804.1"
                     /translation="MSMVRYTRKELNEKFSDKQDAEIKRLLAKGTVPDEQLDLSDIPEI
                     TDWSHAVRHGQFYRPVKQQTSVRLDADVLAWLKAQGKGYQTRMNKILREAMLEDIKNH"
                     /name="BrnA antitoxin family protein"
     gene            complement(5411..6085)
                     /locus_tag="H7R46_RS19175"
                     /old_locus_tag="BE1S18E01_P20070"
     CDS             complement(5411..6085)
                     /locus_tag="H7R46_RS19175"
                     /old_locus_tag="BE1S18E01_P20070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="WP_005065062.1"
                     /translation="MSQKILQRYEQLLTDSASIKAMTKKAYSEYSGSYDTLGDEGDALY
                     LEWKVKVKNLLLLSCGEHSIHYRDFLDAEETQSFDTNTRIISRLIPILKASYDDFKNGF
                     LTSFKQIVQAEVFDSELEQARSLLSSGYKNSAAVIAGVVLETAIKELCLNNGIELEKKK
                     LTRLNEDLAKAGVYNPLQQKLITAMADIRNNAAHGDYDQFTKEDVHRMIEDIERFLLAY
                     SS"
                     /name="HEPN domain-containing protein"
     gene            complement(6137..6538)
                     /locus_tag="H7R46_RS19180"
                     /old_locus_tag="BE1S18E01_P20080"
     CDS             complement(6137..6538)
                     /locus_tag="H7R46_RS19180"
                     /old_locus_tag="BE1S18E01_P20080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005065059.1"
                     /translation="MTDKKQNDHLNLDGINSSYNDGDGLRINNPEDFRSITISNGYFSN
                     NKGNGITIGSPQQSPLEIILTQLAPKLPDTIQPYELASVIQNLLESTNQEEISQKLMTS
                     GLKEKFKDPNLWISFSSLLFSLIFQFSSK"
                     /name="hypothetical protein"
     gene            complement(6630..6932)
                     /locus_tag="H7R46_RS19185"
                     /old_locus_tag="BE1S18E01_P20090"
     CDS             complement(6630..6932)
                     /locus_tag="H7R46_RS19185"
                     /old_locus_tag="BE1S18E01_P20090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_005065057.1"
                     /translation="MAKTLQELLANRSPESQARIQKMADELLLENQLYLIREELEISQK
                     ELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAMGGKLRIDVELPTGKHIGFNV"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(6937..7281)
                     /locus_tag="H7R46_RS19190"
                     /old_locus_tag="BE1S18E01_P20100"
     CDS             complement(6937..7281)
                     /locus_tag="H7R46_RS19190"
                     /old_locus_tag="BE1S18E01_P20100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_005065055.1"
                     /translation="MWTVITTDLFNEWLEQQDEITQEKVLAALVVLQLQGPSLGRPLVD
                     TVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAIVLCIGDKSGKKRFYTEMLAIADKQ
                     YELHLSTLGE"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(7802..8119)
                     /locus_tag="H7R46_RS19195"
                     /old_locus_tag="BE1S18E01_P20110"
     CDS             complement(7802..8119)
                     /locus_tag="H7R46_RS19195"
                     /old_locus_tag="BE1S18E01_P20110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000900860.1"
                     /translation="MLGKNHHFLKKFSISDAAIAYKKPRSTILKYIKSGDFELDYNKLI
                     EYSQLVRKFGELTYPIQDEREQQIKKLKDEVMHLKKQLNFFKYTSISNKNRLLISHKHS
                     I"
                     /name="hypothetical protein"
     gene            complement(8508..9392)
                     /locus_tag="H7R46_RS19200"
                     /old_locus_tag="BE1S18E01_P20120"
     CDS             complement(8508..9392)
                     /locus_tag="H7R46_RS19200"
                     /old_locus_tag="BE1S18E01_P20120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_005065070.1"
                     /translation="MFYLNLKHNIKKEQSSLNAFHYLTRTAHFENQKDNEKLEFMRYGN
                     MPKWAENKPKLFWKSADQFEISRGRTSSTLTIALPKELILEQRAELVQKLIDQFAGQYQ
                     FPYTAVIHNHPSEITGEDQPHLHLMYSERTISDDIERPPEQFFKQYRPKNPTQGGAQKL
                     TADALGFGRNQIKVFREKTQELMNLFLEKYAPTKKVMVYGVEIEVKNQVSCLSNDDFNQ
                     KFGTKLQDVPQIPRWKLYSADPIIQLDVEAQKNTIKKIRNQNNAELYGKEYDLEISRRH
                     VLTQKKDPGFSLD"
                     /name="MobA/MobL family protein"
     gene            9848..10561
                     /locus_tag="H7R46_RS19205"
                     /old_locus_tag="BE1S18E01_P20130"
     CDS             9848..10561
                     /locus_tag="H7R46_RS19205"
                     /old_locus_tag="BE1S18E01_P20130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006581718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_182924265.1"
                     /translation="MAGRKTFALTQQEIIDLDKHIRALPFKRRMQDAFLAMISERESMK
                     QLNEEDLKLIQRCRYERNTYNKRIVQLRLIQKTEPQKLKKLELNILELSKKTDIDAYFA
                     MHDALNEVLKIKRNQTSEKNITAKVERVLNPEKYNQAPLSKSLRKQQDHIKYFIGSLYL
                     NLLEQSGLERSNSNEDLDRMLDLFKQAQSGAEKNNAFSLDQNRKTDILSAKSLYEMEKL
                     IKQAKEDPRNPFNKA"
                     /name="hypothetical protein"
     Typing          102..1017
                     /name="rep_cluster_1226"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.884"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(8512..9392)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccttaaaact aggtttatcg accccaaatt aagtcgattt acaattcaat taaaatagtc
       61 tattgtacaa cctagtttaa ttataaattt attattagaa atgagtgaat taatcgtaaa
      121 ggataatgct ttaattcaag ctagctatac cctagataca gttgaacaaa ggctgatcct
      181 attagccatt gctgaagctc gggaaacagg gcacgggata actgaaaata gccttttaga
      241 agtacatgca agtagttata tcaatacctt taatgtcgag aaacacactg cttataccgt
      301 gcttcgggat gcatctaaaa gcttgtttga tcgctatgtc acgtatcatg acattaatcc
      361 taagactggt aaggatcgta gctttcactg tcgttgggtc gataaaattg gatatgaacc
      421 tcaatccggg atcgttttcc tacgtttcac gcaagacatt gttccactca taactcgact
      481 ggaagaaaac tttactaaat atgaattgga gcaggtttcg agattaacta gctcttatgc
      541 tattcggtta tacgagttat taattcaatg gagatctgct ggtaaaacgc caatttttga
      601 tctatcgatc tttagacaac aacttggtgt cgaagctcat caatacaaaa caatgagtaa
      661 ttttaaaacg tatgttttag attttgccct caagcaagta aatgagttaa ccgatataac
      721 ggctaaatat gaacaacata aaaaaggacg ttcaatttcc ggtttttctt tcagttttaa
      781 gcagaaaaaa accaactctg acaaagtaat taagggaacg gatacattag cccttttcac
      841 caaaatgagt gataaacaac gtcatctctt tgccaacaaa ctttctgaat tacctgagat
      901 ggggcaatat tcagaaggca ccgaaagctt ccaacaattt gctatacgca ttgcagaaat
      961 gctccaagat tatcaaaaat tccaagaatt attcccttat cttcaaaaag ttggctttgc
     1021 acctgcctaa aatttaaact tgatcatttg tttattagac agtctaatag acaacaactt
     1081 ttgacaggtt taggcataga tttacaacat gaaaagcctt acagttctcg agctttctaa
     1141 gctttacaat atcaatagac aaacgattta caacaacata aagaaaggaa ttttaagtaa
     1201 aaattcccag aataagatcg atctagccga ggctatccgt gttttcgggg aacctgtcaa
     1261 aaaacaggat gtaaaagaac ctgtaaaaat agacagtcca aattcggcag aagttttact
     1321 acttagacag cagatagaca tgctgaaaaa ccagcttgat gatgccaaag atcgggaatc
     1381 tttctatcag aaccaaattg aaacgatgca acgcctatta gaagccccaa aacctaatat
     1441 gactacgttt accgatcaag aattagtgca acccagcata gccaaaccag atcccgaacc
     1501 tgacacagca gaagctaaac atgacggatt gactactcca gagcggaggg aaaataagtg
     1561 gattccagtg ccagagcaca ttgaaccaga gcctgagaaa agaggctttt ggagccgttt
     1621 ttttagaccc tatgattaac cacgattgtt taattaatat gaataaagtc gtgaaacttt
     1681 gattaaataa taagcgtgga acgtgaaaaa tcataaaaaa aaagcccacc ttggggaggt
     1741 tgggcaaaaa agaaactaca gcgttgattt ctaaggaaaa ttataacaca tttcgtataa
     1801 ggtgtattat gttaatttta gaaaatctaa aaaagggata gttaaaaact atccttttat
     1861 cttttaaaaa ttatcttaat aataatactg gttttgtaga gcttgataac aatttggtcg
     1921 tcgtactacc aataaaaaac tggcgtatct ttgaatgacc atatgcacca attacaatca
     1981 tatcaataac gtttttttct tgataatcca atatagattt ctcaacatca ccgtcaagca
     2041 tgctatcaat tacatcaaaa ccattatttg caagtagctc ttttgcccaa gtgacttgta
     2101 atgtcatatc actatttggc ttaccaacat ataccacatg agctgtcagg gtttttaata
     2161 aagggctatt tgcaattttg tctacacaaa tacgtgcagt aggacttcca tcaaaagcaa
     2221 ttaaaaaaga cttaggtgga ctaaagggtg aagatgttat taatacgggt ttatgaagtg
     2281 cacgaacaac attttcaata tgggttccaa ctttactgga atcatgctcg gtttcagccc
     2341 catgtttacc cataacaaga acacgaatat cctcttccat tcccatgatt gtctctaaaa
     2401 gactgccgtg gcgttgaaaa ctttgagcat caatagagaa cttctccaat agataattct
     2461 ttgcttcccg aagaagaatt tggccatgct ctagttcaat cttcccctta cgctcttcaa
     2521 tttcaaccaa ttctttgagc aaggtttctc gacttcccaa gccgattgca ccgcttaaat
     2581 cttgtggtgc tttcgctttt ggcttatcaa taacatgtaa taacttaagc ggtgaactaa
     2641 gtttatcgct aaaccaagct gcataatcac aaacggtatt tgttgctaat gatccatcaa
     2701 tacatgcaat aatttttgac atttttcatc cctcttagtg gctatttaag cgatctaatg
     2761 catctggttt atcatggatg ccaaatttat cgactaaggt tttgctggct tcattcattc
     2821 caataagttc gacttctgtt ccttcacgtc taaacttaat gaccacttta tctacggcag
     2881 cgaccgcaga aacatcccaa aagtgagcct gactaacatc aattacaact tttgaaagtg
     2941 cttctttgaa atcaaatgca gaagtaaatt tatctgctga gctgaagaac acttgacctg
     3001 ttaccgtata agtacgggta ttttcaggtt cattaaattc ggactgaatt gccatgtagc
     3061 gttcaatttt gttggcaaaa aataatgcag aaagaagtac accaactaat acgccataag
     3121 ccaaattatg tgtcgcaacg accacgatga ctgtaacgat catcacaata ttgctagata
     3181 agggatattg gcggatattg cgtagtgaat cccagttaaa tgttccaatt gctaccatga
     3241 tcatgacagc aaccagtgca gccattggaa tggctttaag ccagtcactg ataaacacca
     3301 ccataattaa cagaaagacc cctgcactaa atgtagataa acgggtaccc ccaccagatt
     3361 tcacattaat aatcgattga ccaatcatgg cacagcctgc cattccgcct aagaaacctg
     3421 aagcaatatt ggcaacgcct tgtcctttac actcacggtt tttatcacta ttggtatctg
     3481 ttaaatcatc gacaatcgtt gctgtcatca tagattcaag caaacctacg gctgctaatc
     3541 ccagtgaata aggcaaaata atcgttaagg tttcaagatt taaaggaata tcaggaagca
     3601 agaaaattgg aagcgtatcc ggtaattgcc ccatatctcc gacggttctt acatctaaac
     3661 caatgaataa ggcaaagagc gtaagtaaga caatacagat gagaggagag ggtaaaagct
     3721 tccctatcac tggaatataa gggaatagat aaataatggc taaaccacca attgtcatcg
     3781 catagacatg ccatgtcaca ttagtgagtt caggtaattg agccataaaa attagaatag
     3841 ccagggcatt cacaaagcca atcacaactg acttggatac aaaccgcatc aaggcaccta
     3901 attttagata gccggctaag atttgaataa atcctgtcaa aattgtggct gctaataagt
     3961 attgcagacc atgctcttta accagtgtta ccattaataa cgccatggca ccggttgccc
     4021 cagaaatcat accagggcga ccacctacga aagcaatcac cacggcaata cagaatgaag
     4081 cgtataaccc gactttagga tcgacacctg caataataga gaaggcaatt gcttcaggaa
     4141 ttagagccaa agcgacaaca atacccgcaa ggacatcccc gcgaatatta gagaaccatt
     4201 gttctcgaat agtggataac atgaaatagc ctattttttt aaattttaag aaaggctagt
     4261 gatgtgatat atacgacaca caagctacta caccacatca ctagacagtg atgagttatt
     4321 tactgattta agaggtaaat aaacttatgg tggcgtaagt gtcatattaa gaaagatatc
     4381 tattttggag gatttcgggg gcaatcatag cagctagccc cttttctatc caccctaaac
     4441 gcagtactga agtaaaagct aagaattatc atagataatt ttagaattga tttatgccca
     4501 tttaacataa tgggcgttat ccgaaatcgt cttttataag ttaaataaaa tcatatgctt
     4561 aactatttta catcacccgt aaccaccgca aaaatccgct ttggaaacaa agcggatttt
     4621 ttttgagcaa gtttgatact tcttgccaat aattttgact aaaaattact ccaattagct
     4681 tgagtgatac aaatttgtga tacattattg tatatacaaa tttgaatgtt ttttatggaa
     4741 ctgtatttcg aatgggatga ggctaaaaac caaaagaatc agaaaaaaca tgatgtatct
     4801 tttgaaacgg caagcctcgt ttttgaagat cctttacgga tctcaatcca ggatcgacat
     4861 accaatggtg aagaacgttg gcaaacgatc ggtatggtta aaggtgtact gatgttattg
     4921 gtggctcata caatcattga cgatgatgac tgtgagatca tacgaatcat tagtgcaagg
     4981 caagtcacta aagcggaacg caatcaatat gagcatggtt agatatacac gcaaagaact
     5041 gaatgaaaaa ttcagtgaca agcaagatgc tgaaattaaa cgcttgcttg caaaaggaac
     5101 tgtaccagat gaacaattag atctatctga cattcctgag attaccgact ggagtcatgc
     5161 tgttcgccat gggcaatttt atcgtccagt gaagcaacaa acatctgttc gtctagatgc
     5221 agatgtactt gcatggttaa aggctcaagg caaaggctat cagacacgca tgaataaaat
     5281 tctgcgtgaa gcaatgctag aagatattaa gaatcattga tgaaaatggg agctatagac
     5341 tcccattttt gcttcgcata agagatttta tgttaaataa ttaaattttt tctatgtaaa
     5401 tctgacagtc ttacgaagag taagctagta gaaatctttc gatatcttcg atcatcctgt
     5461 gaacatcttc ttttgtaaac tgatcataat ctccatgggc cgcattattt cgaatatcag
     5521 ccatagctgt aattagtttt tgctgtagag ggttataaac tcccgctttt gccaagtctt
     5581 cattcaatcg tgttaatttt ttcttctcta attctattcc attattcaaa catagttctt
     5641 taattgcggt ttctagaact acaccagcaa ttacagcagc tgaattttta taaccactac
     5701 ttaacagcga tcgtgcttgt tctaattcac tatcaaaaac ttcagcttga acaatttgct
     5761 tgaaagaagt taaaaaacca tttttaaaat catcatacga tgcttttaaa attggaatta
     5821 acctactgat tattctagta ttagtatcaa agctctgtgt ttcttcagca tctaaaaaat
     5881 cacggtaatg aatagaatgt tcaccacatg atagtaataa tagattttta actttaactt
     5941 tccattcaag ataaagagca tccccctcat cacccaaagt atcatagcta ccgctatatt
     6001 ctgagtaagc tttttttgtc atagctttga tggaagcaga atcggttagt agttgttcat
     6061 aacgctgtaa tattttttga ctcatgttta ctcagttttt tagggtttcc aaattaagta
     6121 attgaactct ataaatctat ttagatgaaa attggaaaat taatgaaaac aataaactac
     6181 tgaaagatat ccaaagatta ggatctttaa atttttcttt aaggcctgaa gtcattaatt
     6241 tttgagatat ttcttcttga ttagttgatt ccagaaggtt ttgaataact gatgccaatt
     6301 cataaggctg aatagtatct ggaagttttg gggctaattg agtaagaatg atttctaatg
     6361 gtgattgttg cggtgagcca atagtaattc cattcccttt attgtttgag aaatagccat
     6421 tagatatggt aatacttctg aaatcctcag gattatttat tcttagccca tcaccatcat
     6481 tataagatga gttgattcca tcaagattta gatgatcgtt ctgtttttta tcagtcatat
     6541 ataagccttt atcttttgaa tgatttataa cttatatcat actgatcaga ccaaatgaaa
     6601 aaagtgagct taaactcact tttctatatc tatacattga atcctatatg tttcccagtt
     6661 ggaagttcca catcaatacg gagctttcct cccatagctt caacatactt tttcatggtt
     6721 gaaatcttaa gatcattgcc acgattttct attgctgaaa gcgatggctg ttttattccc
     6781 aaagtttcag ccagttcttt ttgagagatt tcgagttctt cacgaataag gtaaagctga
     6841 ttttctaata gcaattcatc agccattttt tgaatacggg cttggctctc aggagagcga
     6901 ttagccaata attcttgtag ggtcttagcc atcagattat tctcctaatg ttgagagatg
     6961 aagttcatat tgcttatctg caattgctag catctctgta taaaaacgct ttttaccgct
     7021 cttatcacca atacacagta caattgcttg tcgtaatgga tcgaatgcaa aaaatgctct
     7081 taatggttta cctcgatgct gaacacgtag ttccttcata ttggtaaatt tagaatcgta
     7141 gacggtatct actaagggac gacctaaact tggtccttgc agttgcagaa caaccaacgc
     7201 agctaagact ttctcttgtg ttatttcatc ttgctgttca agccattcat taaacagatc
     7261 cgttgtaatg actgtccaca tattacaacc ataatataga ttatagtcta tatattaaag
     7321 ctgattgaat aactttgcaa atggcaataa aaaagcccga catcctgtcg ggctttttca
     7381 tatcgggtgc tcgtcttact gatctcgtgc tgcccattcc ttgcgctatt ttcttattct
     7441 tgttgtctgg agacatccag ttcccttatg tattcataat agggctagta ccttggatta
     7501 ccaatcagca aaatccttaa atctctgtaa gctttagctt acaaagttgg cctattttat
     7561 cccaagagtt gtccgcagaa attggggatt atttgtgaag tgtgaccatt gagaaatcag
     7621 gaaaaaatga aaaacgaatg ttttttgatc gattttattg gctaaaagtt acagttttgc
     7681 ttaaaaagaa accgacttgt ttcccagtcg gttttttgtg gttttttagg gggatgaaaa
     7741 atttttaact atatgaaaaa caaggagatt ttaaaaagca tttcgtataa gctctattat
     7801 gttaaatgga atgtttatga gaaattaata gtctattttt gtttgagatt gaagtatatt
     7861 taaaaaagtt aagttgtttt tttaaatgca taacttcatc ttttagtttt ttgatttgtt
     7921 gctctctttc gtcctgtata gggtaagtaa gttcaccaaa ttttctaact aactgactgt
     7981 attcaatcaa tttattgtag tcaagttcga aatctccgct ttttatatat ttaagtatag
     8041 tcgagcgagg ttttttatac gctatagcag catcactgat cgaaaatttt tttaaaaaat
     8101 gatgattttt acctagcata ctatttcact taaggcatta ttaatttcgt atatatatgt
     8161 taataaaatt aacacataca agatataaat ctttaatcat tattggttaa taatcgaacg
     8221 tttactttac acatgcccta ctctgataat ttttctatta ttaaatcaat atgcttttaa
     8281 aaatttatat tattaacata atacacctta tgcgaaaaac ctagatttta catttatggg
     8341 caaaaatcta ggttttcctt attgacctga atcaaaaaca caatctatcc atcattgcag
     8401 cctaaaaaac acaaattttc catgttgtgg ctaaaaatca ctcacaatat ggataagttt
     8461 tttcaagatt ccatgttcaa aaaacacaaa ttttccactt ataaaaatca atctaagcta
     8521 aagccaggat cttttttctg agtaagcaca tggcgtctag atatttctaa atcatattcc
     8581 ttgccataaa gttcagcatt gttctgattt ctaatttttt taattgtgtt tttttgagct
     8641 tcaacatcaa gctgaataat tggatcagcg ctataaagtt tccaacgagg gatttgtggt
     8701 acatcctgta atttggtccc gaatttttga ttgaaatcgt cattagatag gcaactgacc
     8761 tgatttttca cttcaatttc tacaccataa accattactt ttttagttgg tgcatatttt
     8821 tctaaaaaca aattcattaa ttcttgagtt ttttcacgga atactttgat ttgatttcgc
     8881 ccaaaaccta atgcatcagc tgttaacttt tgcgcaccac cttgagttgg attttttggc
     8941 cggtactgtt taaaaaactg ctctggtggt cgttcaatat catctgaaat tgttctttct
     9001 gaatacatca aatgtaaatg tggttgatcc tctccagtaa tttcactggg atgattatga
     9061 atgactgcgg tataaggaaa ttgatattgt ccggcaaact gatctattaa cttctgaacc
     9121 aattctgctc gttgttctag tattaactct tttggtaaag caatggttaa agtagagctt
     9181 gttcttcccc gggaaatttc aaattgatca gcacttttcc aaaaaagttt tggtttattt
     9241 tctgcccatt tgggcatatt tccataccgc ataaactcta atttttcatt gtctttttga
     9301 ttttcaaaat gtgccgtccg agttaaataa tggaatgcat tcagagaact ctgctctttt
     9361 ttaatattat gctttagatt taaataaaac atcagttcac ctcaaacaaa ttacgtgtaa
     9421 cgaaacagca aattttcttg taacaagggg ctaattcttg ttgcagagag cagtctcttc
     9481 ttgttgcaaa catcgatatt tcatcaaaaa agtttgttgc actcgtctaa aattcgctgt
     9541 tttccttgtt gcaccccttt cccatttctt gtaacggcag acattgctta ctttttcacc
     9601 attaagaaaa ataccgttaa aacccgaaga aaaactcata atttaataat taaatcagtg
     9661 gtttatgtag cctccgcagg agcgagtaga agtgtctttt tttaacaatg attgctaaag
     9721 caatcattgt taaaaaaaga cacggaaacg agataaacaa tattttatgt ataacataaa
     9781 atattgtttc aacaccatca gaattccgct aacatcaatt tagataccca aaatcaggaa
     9841 atccgctttg gccggtagaa aaacatttgc cttaacacaa caagaaatca tcgatttgga
     9901 taaacatatt cgagcgctgc cgtttaaaag acgaatgcaa gatgcttttt tagcaatgat
     9961 ttctgaacga gaatcaatga aacaactgaa tgaagaagat ttaaaactta ttcaacgatg
    10021 tcgttatgag cgcaatactt ataataaacg tatagttcaa ttacgactga tccaaaaaac
    10081 cgagccacag aaattaaaaa aattagaatt gaatattcta gaattaagta aaaaaacaga
    10141 tattgatgct tactttgcaa tgcatgatgc attaaatgaa gttttaaaaa ttaaaagaaa
    10201 tcagacttct gaaaagaata ttacagcgaa ggtcgaacga gttttaaacc ctgagaaata
    10261 taaccaggcc ccccttagta aatctttaag aaaacagcaa gaccacatta aatattttat
    10321 cggatcacta tatttaaact tattggaaca aagcggttta gagcgatcaa attcaaacga
    10381 agatttagat cgaatgctcg atttatttaa gcaggcacaa tccggagcag aaaagaacaa
    10441 tgcatttagc ctggatcaaa atagaaaaac agatatttta tcggctaaat cgctatatga
    10501 aatggagaaa ttgattaagc aagccaaaga agatccaagg aatcctttca ataaagctta
    10561 gattgactca aataataggg tttaaataca ctgttgaagc cgtatatgcc ttttttagcc
    10621 taaacctata ttccccatca ataaaaaaat tagagtgctc taaacgcaaa ttagagtatt
    10681 ttggagatca tttgatttta ccgataagaa caaagcgagt gtctacgagc gatccttgga
    10741 aaattttgcc tagactccct gaaaaaccgc tttttaggct gtgaaaccta aataagcatg
    10801 gaaaagttgt agaaatttga gtctagatcg agcgtagcga gtaaaaaagt tttatgagcg
    10861 tagcgaattc cgagttgctt ttgctttttt aaagtcacgt aagtgctcac caaaaaattg
    10921 ccccgacgaa ctgagcgaaa gcgaagttca atagagtttg agcgaagcga aaaccaaggg
    10981 caatttttct ttacctgggc ttttaattat ttttaatctt ttaaatgctt ttaagtaagc
    11041 tgtaaccctt tattcataat agttttagag ttaataaaac taggtttatc gacccataaa
    11101 actaggttta tcgacccata aaactaggtt tatcgaccca taaaactagg tttatcga
//
