LOCUS       NZ_AP022301             7936 bp    DNA     circular CON 14-JUN-2023
DEFINITION  Escherichia coli strain BEC1-S17-ESBL-09 plasmid
            pBEC1-S17-ESBL-09_3, complete sequence.
ACCESSION   NZ_AP022301
VERSION     NZ_AP022301.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194706
            Sequence Read Archive: DRR199542
            Assembly: GCF_014169915.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Escherichia coli str. BEC1-S17-ESBL-09
            isolated from oceanic water
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7936)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 165x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/14/2023 05:40:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,228
            CDSs (total)                      :: 5,100
            Genes (coding)                    :: 4,858
            CDSs (with protein)               :: 4,858
            Genes (RNA)                       :: 128
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 96 of 242
            Pseudo Genes (incomplete)         :: 165 of 242
            Pseudo Genes (internal stop)      :: 37 of 242
            Pseudo Genes (multiple problems)  :: 55 of 242
            Pseudo Genes (short protein)      :: 4 of 242
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022301.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="BEC1-S17-ESBL-09"
                     /isolation_source="sea water"
                     /db_xref="taxon:562"
                     /plasmid="pBEC1-S17-ESBL-09_3"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6306 N 139.7754 E"
                     /collection_date="2017-07-10"
     gene            101..340
                     /locus_tag="H7R48_RS25740"
                     /old_locus_tag="BE1S17E09_P30010"
     CDS             101..340
                     /locus_tag="H7R48_RS25740"
                     /old_locus_tag="BE1S17E09_P30010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 family protein"
                     /protein_id="WP_000703021.1"
                     /translation="MKISDIEKNIYKGLAEFISQKIRDEHDICLGQFESEELLDEILKR
                     VIPVIYNVAIDDAIKSVRNVGDRLEEELDMRKVV"
                     /name="DUF2164 family protein"
     gene            337..699
                     /locus_tag="H7R48_RS25745"
                     /old_locus_tag="BE1S17E09_P30020"
     CDS             337..699
                     /locus_tag="H7R48_RS25745"
                     /old_locus_tag="BE1S17E09_P30020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="WP_000181070.1"
                     /translation="MTNFKSDLNLIRKEWYNAFYSGNTEMLDYLEADWFISTNGQKVIY
                     KKHQLRKIDMRPARILPSIERYEYDVVMREFKGIACVSGKAEIKQNDGNILMAFIENWI
                     KVNGGWKMQFISYEKI"
                     /name="DUF4440 domain-containing protein"
     gene            complement(724..1617)
                     /locus_tag="H7R48_RS25750"
                     /old_locus_tag="BE1S17E09_P30030"
     CDS             complement(724..1617)
                     /locus_tag="H7R48_RS25750"
                     /old_locus_tag="BE1S17E09_P30030"
                     /inference="COORDINATES: protein motif:HMM:NF019619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="WP_001061104.1"
                     /translation="MNNLIKKEIIEKFKKYNFQKYPFVFTDVNYKNLINWNDLNKLLEK
                     DILHYPRVRMANDNFPEIRGYKGFIRYTYSQTGDRTPHINRHQLYKCLRDGATLIVDRC
                     QSFFESVDESRLWLSKELECTCSANLYAAFTATPSFGLHFDNHDVIAVQIEGIKKWKVY
                     NPTYSYPLEDERSFDYLPPNTSPDYEFDITPGQAIYLPAGYWHNVTTQSKHSLHISFTV
                     IRPRRLELFKTLFDELKNNPYMREPIEHGDSLSDKEKIKTIITNAINSFDIQFAENMLK
                     AQSRTYRYKKIKLEHI"
                     /name="cupin domain-containing protein"
     gene            complement(1620..3212)
                     /locus_tag="H7R48_RS25755"
                     /old_locus_tag="BE1S17E09_P30040"
     CDS             complement(1620..3212)
                     /locus_tag="H7R48_RS25755"
                     /old_locus_tag="BE1S17E09_P30040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842142.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_001009226.1"
                     /translation="MNFIEKYIITFNKCNLFFIILLSTISSFLFVLFGYLIKTVIDNKD
                     SIGEINSLLIFIACFLSIRFLMPAGYSISEYLTQKTNIELSVKLREQVIDNIQNSHQEH
                     FLKKNKGELNKVIESMLSSASSLFYTICSDVVPLLIQMIGIIITICISVNTLIAIEFII
                     IMAIYIIFVIKMTQRRFPMMKSVALSSKFASGRMFDMMHMYPMDKAFHTTDKSRKRVIQ
                     AVNTHSEKQRKVNNEFFLFGISSAFLSVIFSSLIILSAYWMFLHGRASRGSIIMLATFL
                     FQVFLPLNRIGYLFRQIKMARTEIDLYCAEINDIKKTNYTDKQYLHVKDNICNIEIYNK
                     SFNHKLILTKGVVTFITGENGSGKTTIAKILSGNITTEENTIKINGIQQEKANSPFVNV
                     LYVPQDLDLMPGTIEENILHYSGINNLNTIKKLLHRFKFDKPLDYEIKGYGHNLSGGQK
                     QKLGVSLTSGKNVDLIIFDEPTKGFDSLGIKIISDYIKEENKEKHIIVISHDEQLINNI
                     PKAQIIDITKHEV"
                     /name="ABC transporter ATP-binding protein"
     gene            complement(3251..3949)
                     /locus_tag="H7R48_RS25760"
                     /old_locus_tag="BE1S17E09_P30050"
     CDS             complement(3251..3949)
                     /locus_tag="H7R48_RS25760"
                     /old_locus_tag="BE1S17E09_P30050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000939065.1"
                     /translation="MLQHKMNSSSYAKVHNVSSLEDIMSYHNDDVLLKFRKEWNVTPEE
                     ADDIFNETKKFIWLASTCLTECYNIKVHEQLQIIDEMWHTFIQFTDAYTSFCEKYLGAY
                     LHHYPNTNDMLKNEIRHVNEHGITFQEYRFNEYKNQIEKIAFYLGHETVAKWYGDYAVR
                     YSIKNINTIRIPKESISSDSYIEKVKSITHLPAAEFVKIIMRKDVWNDNGSVCGCSGKG
                     CGAGCSCNSR"
                     /name="hypothetical protein"
     gene            complement(5314..5505)
                     /locus_tag="H7R48_RS25765"
                     /old_locus_tag="BE1S17E09_P30060"
     CDS             complement(5314..5505)
                     /locus_tag="H7R48_RS25765"
                     /old_locus_tag="BE1S17E09_P30060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5567..5890
                     /locus_tag="H7R48_RS25770"
                     /old_locus_tag="BE1S17E09_P30070"
     CDS             5567..5890
                     /locus_tag="H7R48_RS25770"
                     /old_locus_tag="BE1S17E09_P30070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955993.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVLAALMAIGEELRRLRLAVREQGAR
                     DDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            6099..>6530
                     /locus_tag="H7R48_RS26295"
                     /pseudo=""
     CDS             6099..>6530
                     /locus_tag="H7R48_RS26295"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            6568..7086
                     /locus_tag="H7R48_RS25780"
                     /old_locus_tag="BE1S17E09_P30080"
     CDS             6568..7086
                     /locus_tag="H7R48_RS25780"
                     /old_locus_tag="BE1S17E09_P30080"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673203.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVLFLFTVSG
                     GTLWYLGGMIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            7093..7326
                     /locus_tag="H7R48_RS25785"
                     /old_locus_tag="BE1S17E09_P30090"
     CDS             7093..7326
                     /locus_tag="H7R48_RS25785"
                     /old_locus_tag="BE1S17E09_P30090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137439.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          5159..5238
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.78"
                     /coverage="92.0"
                     /evalue="8.94e-33"
     Typing          complement(4584..5126)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.679"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          5535..5884
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.872"
                     /coverage="99.0"
                     /evalue="9.42e-67"
     Typing          5881..7430
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.787"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cttgcttggg aaattccagg ttgcagtatg gttgagttgt aattttccta tgataggaaa
       61 aatgttatat tattaattta tttgtgaggc ttcataaaga atgaaaattt ctgatattga
      121 aaaaaacata tataaaggac tagctgaatt tatttcacaa aaaatcagag atgaacatga
      181 tatatgcctt ggacagtttg aatcagagga attacttgat gagattctta aaagagtaat
      241 cccagtcata tataatgtgg ctattgatga tgctataaaa tcagtaagga atgttgggga
      301 tagattagag gaggaattag atatgaggaa ggtagtgtga ctaattttaa atcagactta
      361 aatctaataa gaaaagaatg gtataatgca ttttattcag gtaatactga aatgctagat
      421 tacttggaag ctgattggtt tatttcaaca aatggtcaaa aagttatcta taaaaaacat
      481 cagttacgaa agattgatat gcgtccagcg aggattttac ctagcataga aagatatgaa
      541 tatgatgtcg taatgcgtga gtttaaaggc attgcttgtg tttcagggaa agctgaaata
      601 aagcaaaatg atggaaatat attaatggct tttattgaga attggataaa ggttaatggt
      661 ggatggaaaa tgcagtttat atcgtatgaa aaaatctaag gggaagcccc cttagattaa
      721 tggttatata tgttcaagtt ttattttttt atagcgataa gttctgcttt gagctttaag
      781 catgttttct gcaaactgaa tatcaaaact atttatagca ttagttatta tagtttttat
      841 tttttcttta tcagataatg aatcaccatg ctctataggt tcccgcatat atggattgtt
      901 ttttaattca tcaaataatg ttttaaatag ttctaatcga cgaggtctta taactgtaaa
      961 agatatatga agagagtgtt tgctctgagt cgtaacattg tgccagtatc cagcaggaag
     1021 atatatggcc tgacctgggg ttatatcaaa ctcataatct ggggaggtat taggtggtag
     1081 ataatcgaag cttctttcat cttcgagagg gtatgaatag gtagggttgt aaactttcca
     1141 tttttttatt ccctcaattt gaacagctat tacatcatga ttgtcaaaat gaagcccaaa
     1201 gcttggtgtt gctgtaaatg cagcatatag attagcacta catgtacatt ctaactcttt
     1261 agataaccat agtctgcttt catctacaga ttcaaagaat gactggcatc gatctactat
     1321 aagggttgcg ccatcacgta agcatttata tagctgatgg cgatttatat gtggtgttct
     1381 atcgcctgtt tggctatatg tgtatcttat aaatccttta taccccctaa tttcgggaaa
     1441 gttgtcattt gccattctaa ctctaggata gtgcaatata tctttttcaa gcaatttatt
     1501 aagatcattc cagttgatta agtttttata attaacatcc gtgaatacaa acggatattt
     1561 ctggaaatta tatttcttaa atttttctat gatttccttt tttataagat tattcatttt
     1621 taaacctcat gttttgtgat atctataatc tgtgctttag gtatattatt tataagttgt
     1681 tcatcatgag atataacaat gatatgcttt tccttgtttt cctcttttat ataatcactg
     1741 attattttta taccaagtga atcaaaccct tttgtcggtt catcgaaaat aatcaagtct
     1801 acgttttttc cagatgtaag agatactcct aatttttgtt tttgtccacc agataaatta
     1861 tgtccgtaac ctttgatctc ataatctagt ggtttgtcga acttaaatct gtgtagcagc
     1921 tttttaattg tatttaaatt attaatgccg gaataatgta atatattttc ctctatagtt
     1981 ccaggcatga gatctagatc ttgaggaacg tataagacat taacaaaagg agaatttgct
     2041 ttttcttgtt gtatcccatt gatttttatt gtgttttctt ctgttgttat gttgccagag
     2101 agaatttttg caatagttgt ttttcccgaa ccattctcac cggttataaa ggtaacaacg
     2161 ccttttgtta atattaattt atggttgaat gatttgttgt atatttcaat attgcaaata
     2221 ttgtctttta catgaagata ttgcttgtct gtgtaatttg ttttttttat gtcgtttatt
     2281 tcggcacagt ataaatcaat ttctgttctt gccattttta tttgtctaaa tagataacca
     2341 atgcgattta atgggagaaa aacttggaat aaaaaagtgg caagcataat aatactacca
     2401 cggcttgctc taccatgaag aaacatccag tatgctgata ggataatcag actactaaaa
     2461 atgactgaaa gaaaagcgga gctgatacca aacaggaaaa attcattatt tacttttctt
     2521 tgtttttctg aatgtgtatt tacagcttga attactcgtt tacgactttt atccgtggta
     2581 tggaaagctt tatccatagg atacatgtgc atcatatcaa acattcttcc agatgcgaat
     2641 ttggagctaa gtgcaactga tttcatcatt ggaaatctac gttgtgtcat ttttatgaca
     2701 aatattatat agattgccat tattattata aactcaatgg ctattaatgt attaacactt
     2761 atacaaattg ttatgattat tccaatcatt tgtattaata atggtactac atcagaacat
     2821 attgtataaa ataatgatga tgcagaagaa agcatgcttt ctataacctt atttagttct
     2881 cctttatttt tctttaaaaa atgctcttgg tgagaatttt gtatattatc aataacttgc
     2941 tctctaagct ttacagataa ttctatattt gttttttgag tcaagtattc cgatatgcta
     3001 tatccagcag gcataaggaa tctaattgat aagaaacatg ctataaatat taataatgag
     3061 tttatttctc ctattgaatc tttattgtca ataactgttt ttattaaata tccgaataga
     3121 acgaaaagaa aactacttat agttgataat aatataataa aaaacaaatt acatttatta
     3181 aatgtgatga tgtacttttc aataaaattc aatttataat ccttaaagcc cgtatattac
     3241 gggctttatg ttatctagaa ttacatgagc acccagcgcc acatccttta ccgctgcaac
     3301 cacaaacaga accattatca ttccaaacat cttttcgcat tattattttt acaaattctg
     3361 cggctggaag gtgagtgata ctctttactt tttcgatgta agaatcactg gatatggatt
     3421 cttttggaat tcttatagta ttaatgttct ttatgctgta tcttacagca taatcaccat
     3481 accactttgc gacagtttca tgacctaggt aaaaagcgat tttttcaatt tgatttttat
     3541 attcgttaaa acgatattct tggaatgtta taccatgctc attaacatgc cttatctcat
     3601 tttttagcat gtcatttgta tttggataat ggtggaggta agcaccaaga tatttttcac
     3661 aaaagctggt ataagcatct gtaaattgaa taaacgtatg ccacatttca tctataattt
     3721 gtaattgctc gtgaactttt atattgtagc attccgttag gcatgttgag gctagccaga
     3781 tgaatttctt tgtttcatta aagatatcat cagcttcttc tggtgttaca ttccattctt
     3841 tacgaaattt tagaagaaca tcatcattgt gataactcat gatatcttct aatgagctaa
     3901 cattatgaac ttttgcataa gaactgctgt tcatcttatg ttgtagcata acacccccaa
     3961 ttatatattt gaatgtagat gagaatagtt atcatttgtt atggtattgt taaatttgtg
     4021 cttgtgtcaa tatgcctaat tctataaatg agatagtaat caagtttttt aatggttata
     4081 ggatccatta gtaatacaag gtttttgcag ccagttttta cgggtagggg ctgcaaggaa
     4141 aacaaaattt tcccactcta agagaagaga attgagttgg ttgaatgagt ggatgcatct
     4201 agggccgcta attcaccgag tgtaaaggct gtacgaaatg tgttgcagtc cgataatagc
     4261 cccctccatt gacgttttac acatgtgttg taggttccag gagtgggcgg atctcaaaaa
     4321 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgcc agtaaacgaa
     4381 aaaaccgcct ggggaggcgg tttagtcgaa ggttaagtca gttggggact gcttaaccgg
     4441 gtaactggct tggtggagca cagataccaa atactgtcct tctagtgtag ccgcagttag
     4501 gccaccactt caagaactct taatatctca atccaccttg tccagttacc agtggctgct
     4561 gccagtggcg ctttgtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     4621 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     4681 tacaccgagc cgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     4741 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     4801 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     4861 gagcgtcgat ttttatgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccaacggc
     4921 gcagcctttt cctggttctc gttttttgct cacatgtttc ttttggcgtt atcccctgat
     4981 tctgtggata accgcatctc cgcttttgag tgagcagaca ccgctcgccg cagccgaacg
     5041 accgagtgta gcgagtcagt gagcgaggaa gcggaagagc gccggaacgt gcattttctc
     5101 cttacgcatc tgtgcggcat ttcacatcgg acatggtgcg ctttccatac aattcgtact
     5161 gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg ttcagggctt
     5221 cgccccgaaa ccccctgacg cgccctgagg ggcttgtctg ctcccggcat ccgctcacag
     5281 acaagctgtt accgtctccg ggagctgtat gtgtcagagg ttttcaccgt catccccgaa
     5341 gcgtgcgagg cagctgcggt aaagctcgtc ggcgtggtcg tgaagtgatt cacaaatatc
     5401 ggcctgttca tctgcgtcca gttcgttgag tttctccagc agcgttaatg tctggcttct
     5461 gataaaacgg gccatgttaa gggcggtttt ttcctgttta gtcactgatg cctccgtgta
     5521 agggggattt ctgtatgggg taatgatacc gatgaaacgc gagaggatgc tcaccatacg
     5581 ggttactgat gatgaacatg cccggttact ggaacgctgt gagggtaaac agctggcggt
     5641 atggatgcgg cgggtctgcc tgggtgagcc ggttgcacgc tccgggaagc tgccgacact
     5701 ggcaccgccg ttactgcgtc agctggccgc catcgggaat aacctgaatc agacagcgag
     5761 gaaggtgaac agcgggcagt ggtcttccgg tgatcgggtt caggtgttgg ctgcgctgat
     5821 ggccatcggg gaggagctgc gtcggctgcg tctggccgtc agggaacagg gcgcgcggga
     5881 tgattgttaa atttcatgcc aggggaaaag gcggtggcag tggtccggtt gattacctgc
     5941 tgggaaggga gcgaaaccgg gaaggggcaa cggtgcttca gggtaatccg gaagaagtcc
     6001 gggaactcat cgatgccacg ccatttgcga agaaatacac gtcaggtgtt ctgtcgttcg
     6061 cggagaagga gctgccgccg ggaggacgtg aaaaggtgat ggcgagcttt gagcgtgtac
     6121 tgatgcccgg tctcgaaaag aatcagtaca gcatcctgtg ggtggagcac caggacaagg
     6181 gacggcttga gctgaatttt gtcattccga acatggagct acagaccgga aaacgcctcc
     6241 agccgtacta cgaccgggca gacaggccga gaattgatgc ctggcagacg ctggtgaatc
     6301 accattacgg gctgcatgac cccaacgccc cggagaaccg ccggatactg actctccctg
     6361 ataacctgcc ggaaacgaaa caggcgcttg cagagagcgt cacacgcggg atagacgctc
     6421 tttatcatgt cggggagata aaaggtcgtc aggatgtgat tcaggcgctc acagaggcag
     6481 ggcttgaagt ggtcagggtg atgcgaagca gtatcagcat tgcagacccg aacggtggga
     6541 agaatatcag gctgaaagga gcattttatg agcaatcttt tacagacggg cgcggagttc
     6601 gagaaaaagc tgaaagagag agccgaatct acagagacaa tgctgaacga cgagttcagc
     6661 aagctcgaaa aatctgtaag caaggctgtg acatcaaacg agacgaaaat caaagacgct
     6721 atagccctgt tcacagcttc gacagaggaa tcactgaaaa aacaccggga aggggtgaaa
     6781 gaggcgatga tgcagcacag gaaggacgtg ttaaagctgg cagggaatac gggcatgatg
     6841 ttactgggga tggtcctttt cctgtttacc gtgagtggcg ggacgctctg gtatcttggc
     6901 gggatgatac aggcgaacct ggaagaaatc aggatacagg aagaaacatt gcagaaactg
     6961 aacgcgaaga catggggcgt ggagtttgtg caggacggga gaaggaaatt tctggttatc
     7021 cctcagggga aatcggcaac ggtgattccc tatcagggga aggactgggt acaactgacg
     7081 gagtgacaca gagtgacgga gctggaaaga catttgctga acgccttaga gcaactgcaa
     7141 caggactata tgcagcggct gaacgaatgg gagagcgcct tcgtggaatt gcagaaaatg
     7201 ttttcgctta cgcaacggga caacgcgatg ctgaacgagc gggtcatgca gttgagtcag
     7261 caggtgcagc acttgagcga gcagacagaa cgcttgagcc agttatacag cgagaacagg
     7321 agataaggga tgaacggctg atacaggaac gcgaacatga gttatccctg gagcgtgagc
     7381 gccagctgga aatacaggaa cggacacagg atggcccttc gctgggatgg taatcagaga
     7441 caggctaaaa tgttgtcagc gcgtttccgg tgcatttttt gggataatgg ttggttcggg
     7501 ggcaccggga acgcgcttca gtcagacctt gctgaaaaca caggaacgcg ttaaaacgca
     7561 tacgggcgcg attctggtgt gtcttttgtt gctgtgtttt gcctctattg ccagatcgcg
     7621 ctcattcccc tgaacttctg ttttcccgct ccggtccccg tcttcttcgc ttccgaaagc
     7681 atgaaaaaaa tccgtttcag ctgtctctgg ctggcaattc ctgcgtgatt cacatggctg
     7741 catagctatg catgtggtta aaatttaccg ggtgcgatcg cggcagtttt tcgggtggtt
     7801 tgttgccatt tttacctgtc tgctgccgtg atcgcgccga acgcgtttca gcggtgcgta
     7861 caataaggga ttatggtaaa ttatcttgtt ccttagagaa tgatttgagg tggtatcctg
     7921 cacttattta aatatg
//
