LOCUS       NZ_AP022286             4897 bp    DNA     circular CON 09-DEC-2023
DEFINITION  Aeromonas veronii strain WP8-W19-CRE-03 plasmid pWP8-W19-CRE-03_5,
            complete sequence.
ACCESSION   NZ_AP022286
VERSION     NZ_AP022286.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194639
            Sequence Read Archive: DRR199475
            Assembly: GCF_014169835.1
KEYWORDS    RefSeq.
SOURCE      Aeromonas veronii (Aeromonas ichthiosmia)
  ORGANISM  Aeromonas veronii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Aeromonadales;
            Aeromonadaceae; Aeromonas.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Aeromonas veronii str. WP8-W19-CRE-03
            isolated from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4897)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 394x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/09/2023 11:55:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,463
            CDSs (total)                      :: 4,312
            Genes (coding)                    :: 4,234
            CDSs (with protein)               :: 4,234
            Genes (RNA)                       :: 151
            rRNAs                             :: 11, 10, 10 (5S, 16S, 23S)
            complete rRNAs                    :: 11, 10, 10 (5S, 16S, 23S)
            tRNAs                             :: 115
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 24 of 79
            Pseudo Genes (incomplete)         :: 65 of 79
            Pseudo Genes (internal stop)      :: 5 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022286.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Aeromonas veronii"
                     /mol_type="genomic DNA"
                     /strain="WP8-W19-CRE-03"
                     /isolation_source="wastewater"
                     /db_xref="taxon:654"
                     /plasmid="pWP8-W19-CRE-03_5"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6316 N 139.7460 E"
                     /collection_date="2019-02-05"
     gene            101..1087
                     /locus_tag="H7R74_RS22215"
                     /old_locus_tag="WP8W19C03_P50010"
     CDS             101..1087
                     /locus_tag="H7R74_RS22215"
                     /old_locus_tag="WP8W19C03_P50010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_094965910.1"
                     /translation="MTEKAFLRNLPHRPYCTDDKSSGLLIRPQATALGYRHIQYNPPPH
                     VASLVFDLDKPDSYHAWQDAGLPAPHWISVNRTNGHAHIGYMLATPVARTSAARQKPLR
                     YLAAIEHVLAKRLGADMGYAGLITKNPTHSDWLTIWHQIEPYSLDYLAEFCPDADLAAY
                     SRRSRKEATGLGRNVTVFDNVREWAYTAIRAHWRPNGYDAWLCAVQAACECTNVFGLEQ
                     GGPLPHSEIKATAKSIARWTWRNLTPSTFADYVDRTHTPEIQAVRGAKGGRIGGKVSKG
                     GGRPSKAASLLPVVLQLKAQGYSNRDIAEDLQISAGSVSNYLRRDRE"
                     /name="replication initiation protein"
     gene            complement(1240..1530)
                     /locus_tag="H7R74_RS22220"
                     /old_locus_tag="WP8W19C03_P50020"
     CDS             complement(1240..1530)
                     /locus_tag="H7R74_RS22220"
                     /old_locus_tag="WP8W19C03_P50020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094965911.1"
                     /translation="MKDNADKGTGDMFGIKRGRGRPKTGSAKTGAERQAAYRTKQQENN
                     VTVTINRGLLEGLDAQMQAIRDGGSAVVLTSEQAGEILRALRTAEKKQLRG"
                     /name="hypothetical protein"
     gene            1729..2067
                     /locus_tag="H7R74_RS22225"
                     /old_locus_tag="WP8W19C03_P50030"
     CDS             1729..2067
                     /locus_tag="H7R74_RS22225"
                     /old_locus_tag="WP8W19C03_P50030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019820367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_094461467.1"
                     /translation="MPTEQPKRLKTIKVRVTETEWASLRAACPKAQLAEWMREHCLGVV
                     PQQRRTPPPTIDPALLRQVAGIGNNLNQIARRINASPLTTLDKVEYLAALAAVARELEG
                     LRRDRQNP"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2267..>2701
                     /locus_tag="H7R74_RS22445"
                     /pseudo=""
     CDS             2267..>2701
                     /locus_tag="H7R74_RS22445"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2754..3170
                     /locus_tag="H7R74_RS22450"
                     /old_locus_tag="WP8W19C03_P50040"
     CDS             2754..3170
                     /locus_tag="H7R74_RS22450"
                     /old_locus_tag="WP8W19C03_P50040"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_232098442.1"
                     /translation="MAKTFEQTSKQQADDIESSLKPEFERHERAIKEALDSSRQSINDA
                     IQDQQKLIGWLLLKSWGVMLISGLLLLAATSGTLWYQGNLIAERQATLETLGKKGGKLI
                     FNKCEDENGKIRLCIRMNMDEGKFGDDYMIPKGY"
                     /name="MbeB family mobilization protein"
     gene            3174..3407
                     /locus_tag="H7R74_RS22455"
                     /old_locus_tag="WP8W19C03_P50050"
     CDS             3174..3407
                     /locus_tag="H7R74_RS22455"
                     /old_locus_tag="WP8W19C03_P50050"
                     /inference="COORDINATES: protein motif:HMM:NF016769.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD/MobD family mobilization/exclusion protein"
                     /protein_id="WP_153913308.1"
                     /translation="MTELERHLLNALETLQSEQDRQLSGLQTACESLLRQFETTKKENA
                     SLLGEVKVLRQQCEHLNEQVSSLATQVASLKA"
                     /name="MbeD/MobD family mobilization/exclusion protein"
     gene            3743..3982
                     /locus_tag="H7R74_RS22235"
                     /old_locus_tag="WP8W19C03_P50070"
     CDS             3743..3982
                     /locus_tag="H7R74_RS22235"
                     /old_locus_tag="WP8W19C03_P50070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="WP_094965907.1"
                     /translation="MVAAAMRSKRTVSVTLEPSLLERARAAGINLSATLAAALQHEISG
                     SEAERWKRENSAAIQELNRITEEHGLLSDDYRAF"
                     /name="type II toxin-antitoxin system CcdA family
                     antitoxin"
     gene            3982..4290
                     /locus_tag="H7R74_RS22240"
                     /old_locus_tag="WP8W19C03_P50080"
     CDS             3982..4290
                     /locus_tag="H7R74_RS22240"
                     /old_locus_tag="WP8W19C03_P50080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="WP_094965908.1"
                     /translation="MQFTIYRNKGNARAYPYLLDVQSDIIGELATRIVIPLFPLAAFTG
                     RPAQRLNPVIIVEGDKYLVMTQDMAAVRQTQLGEEVTGVQESRQLIKDAIDFLMDGV"
                     /name="CcdB family protein"
     gene            4405..4836
                     /locus_tag="H7R74_RS22245"
     CDS             4405..4836
                     /locus_tag="H7R74_RS22245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019535530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="WP_182947070.1"
                     /translation="MNIPTHLKHKPVLEVINYDQIDGPYANDSDAMGLSIGIAQWNSPG
                     WTDLSAKVWRHTGEKWSRQSEELPLHRVIDLTTLICIAMEYCRNGRLSSSDDFPVSMST
                     DNADLGHHVELMKKELKSGSKYLDESLKRLSLELKRLGY"
                     /name="DUF6530 family protein"
     Typing          2589..3616
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.244"
                     /coverage="100.0"
                     /evalue="1.24e-180"
ORIGIN
        1 ggcggatctg cgaaaaacgg gggctgtgac gaatcacaag agaagtctac cacatggatc
       61 acaccaaaca accctgcacc gccaataagc gcggtgatcg atgacagaaa aagcctttct
      121 caggaatctc ccccatcggc cctattgcac tgacgataaa tcgtcagggc tgctcattcg
      181 cccgcaggct accgcactgg gctatcgcca tatccagtac aacccaccgc cgcatgtggc
      241 gtctttggtg ttcgacttgg ataaaccgga cagttaccac gcttggcagg atgcaggctt
      301 gccagccccg cactggatca gcgttaaccg cacgaacggc cacgcccata ttgggtacat
      361 gctggcgacc ccagtagccc gtaccagcgc cgcccggcaa aagccgctgc gatacctagc
      421 cgccatagaa cacgttctag cgaagcggct gggggccgac atggggtatg cggggctgat
      481 caccaaaaac cccacccata gcgattggct caccatctgg caccaaatcg agccctacag
      541 cctcgactat ctcgcagagt tctgcccgga tgccgatttg gcggcctaca gccgccgcag
      601 tcgcaaagag gcgactgggc taggccgtaa cgtcactgtg ttcgacaacg tgcgggaatg
      661 ggcctacacg gcgattagag cgcattggcg gcctaatggg tacgatgcat ggctctgtgc
      721 cgtgcaagct gcctgtgagt gcaccaacgt gtttggcttg gagcagggtg gcccgttgcc
      781 ccatagcgag atcaaggcca cggccaagag catcgcccgt tggacgtggc ggaatctaac
      841 cccgtccacg ttcgcggatt atgttgatcg gacgcataca ccagagattc aggccgtcag
      901 aggggctaag ggcgggcgta tcggtggcaa ggtatccaag ggcggaggca ggccgagcaa
      961 agctgcttct ctcctacctg tggtgctaca actcaaggcg cagggttaca gcaatcgcga
     1021 tattgcggag gatctgcaaa tttcggctgg cagtgtgtcc aactacctcc gccgtgatcg
     1081 tgagtgatca aaaacaaagc ctatatcaga taacagcgcc cgtagggcgt tccttgagcc
     1141 ctaaaagcat tcaagagaga ggatcttggc gagggggggt aacggcgatc ccgtagggct
     1201 gtccatcagg ccatgtgttc ggcctgtggg cagtagctgt tatccccgga gctgtttttt
     1261 ctctgctgtt cgtagcgccc gcaagatctc cccggcctgt tccgaggtca gcaccaccgc
     1321 agaacccccg tctctgatcg cctgcatctg ggcatccagt ccttccaata gccctctatt
     1381 tatcgtcaca gtaacgttat tctcctgctg ttttgtccgg taggccgctt gtcgttctgc
     1441 ccctgttttg gctgatccgg ttttgggtct accccgtccg cgcttgatgc caaacatgtc
     1501 tccggttccc ttatcagcgt tgtctttcat cgtcctaatt cccgtcacag tgaccataaa
     1561 tattatctgt cactgtgaca caaaaaccat taatccgcac cctcaaaatc caacccatgt
     1621 taaagtacta agcaaggaca cacagggggc ccgcttcgcg gtctatcccc tgtggccttg
     1681 ttcagggggc aagccccccg aaacccccga aaaaaacgga ggccatcgat gccgacagag
     1741 cagccaaaac ggctcaaaac gatcaaggtc agagtcactg aaaccgagtg ggccagcttg
     1801 cgggcggcct gccccaaagc ccagttggcg gagtggatgc gcgagcactg cttgggggtt
     1861 gttccccagc agcggcgcac cccaccgcca accatcgacc cggccttgct ccggcaggtg
     1921 gccgggatcg ggaacaacct caaccagatc gcccggcgca tcaatgccag cccgctgacc
     1981 acgttggaca aggtggaata ccttgcggcg ttagccgccg tggcccgaga actggagggg
     2041 ctacgccgtg atcgtcaaaa tccatagccg gggagtcggt cggggatctg gcccggtgga
     2101 atacctgctt ggcaaagacg ggaaccggag cggggccacg ctggatcgcg gcgaccccga
     2161 gatggtcagg gagttgatag acggcagccg ttacgcccag cggtacacca gcggcgtgct
     2221 gtcgttccat gaaccggact tgccgaggga gaccaaggat cggttgatgg acgagttcga
     2281 gcaagccctg ttgcccggac tggacgccga tcagtatgcg gtgctgtggg tcgagcaccg
     2341 agacaaagac cgcctagagc tgaatttcgt ggtgccgaac gtcgaactgt tgtccggcaa
     2401 acgccttcag ccgtatttcc acggggcgga cggccaccgg atcaacgcat ggcgaacggc
     2461 catcaatggc gacttgggat tgcacgaccc agacgaccca atcaatcggc aggccgtgac
     2521 tacggccaaa gacctacccc ggaacaagct ggaagccgaa caagccatta caggcggctt
     2581 gctgggcatg gctgcggcgg ggctggttaa aaaccgggag gacgtgctca agaccctgac
     2641 cggggcgggg ttcgacgtta ccaggacaac caagaacagt atcagcattg ccgaccctga
     2701 agggggccgg aatatccgcc taaagggggc gatctatgag caagatttta gacatggcaa
     2761 agaccttcga gcagacatcg aagcagcaag ccgacgatat agagagcagt ctgaagccag
     2821 agttcgagag gcacgagcga gctatcaaag aagcgttgga ctcaagcagg cagagcatca
     2881 acgacgccat ccaagaccag cagaagctga tcggctggct cctgctcaag agctggggag
     2941 tgatgttgat ctcgggcttg ctgttgctgg ccgcgacgag cgggacgctg tggtatcagg
     3001 ggaacctgat cgccgagcgg caggccacct tggagacgtt gggcaaaaag ggcgggaaat
     3061 tgatcttcaa caagtgcgag gacgagaacg ggaagatccg gctttgcatc aggatgaaca
     3121 tggacgaagg gaagttcggg gacgattaca tgataccgaa gggctactaa acgatgaccg
     3181 agctagaacg gcacttattg aacgcgctag agaccttgca gagcgagcaa gatcggcaat
     3241 tgagcggctt gcaaacagcg tgcgagagct tgctacggca gttcgagacg acgaaaaaag
     3301 aaaacgcgag cttgctggga gaagtgaaag tcttgcgcca gcagtgcgag cacttgaacg
     3361 agcaggtcag cagcttggca acgcaggtcg ccagcttgaa ggcatgatcg cgcagcagac
     3421 ggcgcagacg caggaggggt tattggacga ataccagcag gcccacgcag aacgggatct
     3481 ctatttcaat cggaagcacg agcagaaaga gatctccggc ttgccgatag acaaggccag
     3541 agagtaccta caggaaggac tggaggggcg gatagagcgg cagcaggtcc gcgaacgggg
     3601 gcgtggcttt gagatgtagc tgccgcgctg cgcttggcgg ccccagcccc gagcgcgaca
     3661 gacttcgcta gtcgcacccg tcgcaagggc gtgatattat catacacgtg ctatcgcacg
     3721 tgttaattta ttggaggtca tcatggtcgc agcagcaatg cgcagcaaga gaacggtcag
     3781 cgtcacgctg gagcccagct tgctggaacg agccagagcg gcggggatca acctgtccgc
     3841 cacccttgca gccgcactac agcatgaaat cagcggatca gaagcagaac gctggaagcg
     3901 tgagaatagt gcagccatac aggagcttaa ccgcatcacc gaggagcacg gtttgctttc
     3961 cgacgattac agggcgttct gatgcagttc acgatctacc gcaacaaggg caatgcccgc
     4021 gcttacccct atctgctgga tgtgcaaagc gacatcatcg gcgagctggc caccagaata
     4081 gtgatcccac tgttcccgct ggcggccttc accgggcggc cagcgcaacg gctgaatccg
     4141 gtaattatcg ttgaggggga taagtatctg gtgatgacgc aggacatggc agcggtgagg
     4201 cagacgcagc ttggggaaga agtcacagga gtgcaggagt ctcgtcagct catcaaggac
     4261 gcaatagatt tcctgatgga tggtgtttga ggctttaaag ggaggcagat atttcggcgg
     4321 ttccggcgta caaccccata gacattacca actaaactca gtaatgtttg tgaaattagt
     4381 aaacttgata atctggggct cgctatgaac attccaactc atttgaagca caagccggta
     4441 ctggaagtta ttaactatga ccagattgat ggtccatatg ctaatgactc tgatgcaatg
     4501 ggactgtcaa ttggcatcgc acaatggaac tcacctggat ggactgacct ctcagccaaa
     4561 gtatggcgtc atacaggcga gaaatggtca agacagtcag aagaactacc actgcatcgt
     4621 gttatcgact taacgacatt gatatgtatc gcgatggaat actgtcgcaa tggtcgcttg
     4681 tcatctagcg acgatttccc ggttagcatg tctactgata acgctgattt ggggcatcat
     4741 gttgagctaa tgaaaaaaga gctcaagagc gggagtaaat atctcgatga aagtctaaaa
     4801 cgactgtctt tagagctaaa aaggctgggt tactaacggg gctatgcggt aacttattgc
     4861 tcataaaaag acaaaccccc gttatcgctg gaatctt
//
