LOCUS       NZ_AP022285             5914 bp    DNA     circular CON 09-DEC-2023
DEFINITION  Aeromonas veronii strain WP8-W19-CRE-03 plasmid pWP8-W19-CRE-03_4,
            complete sequence.
ACCESSION   NZ_AP022285
VERSION     NZ_AP022285.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194639
            Sequence Read Archive: DRR199475
            Assembly: GCF_014169835.1
KEYWORDS    RefSeq.
SOURCE      Aeromonas veronii (Aeromonas ichthiosmia)
  ORGANISM  Aeromonas veronii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Aeromonadales;
            Aeromonadaceae; Aeromonas.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Aeromonas veronii str. WP8-W19-CRE-03
            isolated from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5914)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 394x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/09/2023 11:55:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,463
            CDSs (total)                      :: 4,312
            Genes (coding)                    :: 4,234
            CDSs (with protein)               :: 4,234
            Genes (RNA)                       :: 151
            rRNAs                             :: 11, 10, 10 (5S, 16S, 23S)
            complete rRNAs                    :: 11, 10, 10 (5S, 16S, 23S)
            tRNAs                             :: 115
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 24 of 79
            Pseudo Genes (incomplete)         :: 65 of 79
            Pseudo Genes (internal stop)      :: 5 of 79
            Pseudo Genes (multiple problems)  :: 13 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022285.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5914
                     /organism="Aeromonas veronii"
                     /mol_type="genomic DNA"
                     /strain="WP8-W19-CRE-03"
                     /isolation_source="wastewater"
                     /db_xref="taxon:654"
                     /plasmid="pWP8-W19-CRE-03_4"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6316 N 139.7460 E"
                     /collection_date="2019-02-05"
     gene            complement(375..812)
                     /locus_tag="H7R74_RS22180"
     CDS             complement(375..812)
                     /locus_tag="H7R74_RS22180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019535530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="WP_182963894.1"
                     /translation="MNIPSHLKHKPILEVANYDKIDGPYADDTDAKGLSIGIAQWNSPG
                     WTTLSAKVWRHTGEKWSRQSEELPLHRIIDLTTLICIAMKYCRNGGLSSSDDFPVSMAT
                     DNPDLAHHLLLMKKELDDKVQYEFLDSRLKRLSLELKKLGY"
                     /name="DUF6530 family protein"
     gene            complement(1047..1355)
                     /locus_tag="H7R74_RS22430"
                     /old_locus_tag="WP8W19C03_P40020"
     CDS             complement(1047..1355)
                     /locus_tag="H7R74_RS22430"
                     /old_locus_tag="WP8W19C03_P40020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_232098440.1"
                     /translation="MTELERVLLAKLEQIEQRHEQQTEDLRQQLQQQAHSLIALQKVCS
                     DALRSCGKLCSDLHEEIRTLQSGVTHSNKVTSAALGSLNSSVSALNKALENLQSAQG"
                     /name="mobilization protein"
     gene            complement(1356..1913)
                     /locus_tag="H7R74_RS22435"
                     /old_locus_tag="WP8W19C03_P40030"
     CDS             complement(1356..1913)
                     /locus_tag="H7R74_RS22435"
                     /old_locus_tag="WP8W19C03_P40030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011116724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1640 domain-containing protein"
                     /protein_id="WP_232098441.1"
                     /translation="MESLGQHKARLSEKLKQRQEQELGEVEKLAQALLETLRRDLQTRI
                     SGELNTTVTAIKDEIEDLPQQITDSTEPLKEVLEQARSEGEALKLVSSVWFKATLLGMF
                     CGVGLLVGSWFGMSWIWDQIEQDMGTLRTLRQEISSAQSTLDELPKGVSYVKGRNGTGY
                     LIARKIGTPYKTEAGKMAVEVR"
                     /name="DUF1640 domain-containing protein"
     gene            complement(<1945..2628)
                     /locus_tag="H7R74_RS22440"
                     /pseudo=""
     CDS             complement(<1945..2628)
                     /locus_tag="H7R74_RS22440"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2618..3028)
                     /locus_tag="H7R74_RS22190"
                     /old_locus_tag="WP8W19C03_P40040"
     CDS             complement(2618..3028)
                     /locus_tag="H7R74_RS22190"
                     /old_locus_tag="WP8W19C03_P40040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_182963896.1"
                     /translation="MTKTARTTTVSVRIDKPTAAEWREKAEASDLSLSDWIRGAIDANQ
                     QTHLPTPRKRAVRVRDTSHDADPVLMRQLAGMSNNLNQIARALNECRLIGSPVQVVEVL
                     ALLRSIEGQAAHLLPQLPPPPKPNRKGESDAH"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3238..3561
                     /locus_tag="H7R74_RS22195"
                     /old_locus_tag="WP8W19C03_P40050"
     CDS             3238..3561
                     /locus_tag="H7R74_RS22195"
                     /old_locus_tag="WP8W19C03_P40050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_182963890.1"
                     /translation="MKDSTDKGTGDMFGEKRGRGRPKTGTAKTGAERQAAFRAKQQDNS
                     VTVTINRELLDGLDAQMQAIRDRGSVVVLTPEQAGEILKAIRKSSLKQLRGQRPRPQAK
                     PTA"
                     /name="hypothetical protein"
     gene            complement(3676..4665)
                     /locus_tag="H7R74_RS22200"
                     /old_locus_tag="WP8W19C03_P40060"
     CDS             complement(3676..4665)
                     /locus_tag="H7R74_RS22200"
                     /old_locus_tag="WP8W19C03_P40060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_182963891.1"
                     /translation="MVKPALQSAAFVERLPRRPYCTDDPAQGLLIRPQATALAYRHIQH
                     NPPPHVACLVFDVDSPDGYEAWKDAGLPAPNWITFNARNGHAHYGYYLAAVVARTCAAK
                     QKPLRYLAAIEHVLAKRLGADMGYAGLITQNPVHSDWWTIWHHSEPFSLDYLAEFCPDA
                     DLAAYSRRSRKEVGGLGRNVTVFDNVREWAYSAVRAYWRPNGYEAWANAVRAACDSANA
                     FGREQGGPLPVSEIKATAKSIARWVWNRFTPAGFSQVQAHRGAKGGRIGGPIGGKVSKR
                     PTKSGKARADLLPEVIRLKAMGYSNRDIGEDLGIGSATVSRYLKRDPE"
                     /name="replication initiation protein"
     gene            complement(4827..5249)
                     /locus_tag="H7R74_RS22205"
                     /old_locus_tag="WP8W19C03_P40070"
     CDS             complement(4827..5249)
                     /locus_tag="H7R74_RS22205"
                     /old_locus_tag="WP8W19C03_P40070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="WP_182963892.1"
                     /translation="MELTDDMIERANQCGSEERKRNPVATSARYDRRIGRVVIALSSGL
                     ELAFAPHLVQGLEAAKPSDLLDVEISPSGLGVHFPKLDADLYLPALLEGLFGSANWAAS
                     RMGSKGGMAKSPAKVAAARANGSKGGRPRKSVLVVA"
                     /name="DUF2442 domain-containing protein"
     gene            complement(5233..5475)
                     /locus_tag="H7R74_RS22210"
     CDS             complement(5233..5475)
                     /locus_tag="H7R74_RS22210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="WP_182963893.1"
                     /translation="MPTVLIIGGWRVVIYPNDHRPAHVHVIGGGGEAVFNLAGELELRE
                     NHGFDSKSLRRIWAELQNNQAHLITKWEDIHGTNR"
                     /name="DUF4160 domain-containing protein"
     Typing          complement(3729..4665)
                     /name="rep_cluster_1069"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.436"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          complement(1031..2628)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.745"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 tcaaactgtg gtgaaaacaa agtttttaac aatacctgtc gcggttgcgg cgctcaattc
       61 ataggatgat gttaaatcat gaacactagg cgaagtaaca atgcaaatac aattgagctc
      121 cctcacgtat gtccttgcaa agattttgac tgcaatgaaa accctgtggt tgcttatagt
      181 tggcaagagg ttgagaaata ttttggattc aggaccttac ctgctggcat tgccccgcaa
      241 ccgtggtgta agaaatgcag agcaaagggt aactcggcac aaaaatcaac ccagtaataa
      301 ttgttgtgac tttggcagca ttaactattg ttaatgctgc caaagcattt cggctatttt
      361 tattctaaat taaattagta accaagtttt ttaagttcta aagataatct ttttagtctg
      421 ctatctaaaa attcatattg tactttatca tccaactctt ttttcatcag caaaaggtga
      481 tgtgctaaat cagggttatc tgtcgccata ctaactggaa aatcatcact ggatgacaag
      541 ccaccattac gacaatattt cattgcgata catatcaatg tggttagatc aataatccga
      601 tgaagcggca actcttccga ctgtcttgac catttttccc ctgtatgacg ccagacctta
      661 gccgaaagag tggtccatcc aggcgaattc cattgtgcaa tgccaattga tagcccctta
      721 gcatctgtat catcagcgta tggaccatca atcttgtcgt agttcgcaac ttccagtatt
      781 ggcttgtgct tcaaatgaga tggtatgttc atggcgcacc tgtcagtgaa tttactaatt
      841 atactaacat tactaaattt tataaaacac gtcaaatgaa gttgccacag gacaacacca
      901 gatcgagggc cacctcaaac caatgctggt gcgggagccg ccaagcaacg cgcggcagcc
      961 ctacagctca aatcctctat accgctcata ctccagcgat aattccagct ctttgctctc
     1021 cttgagctcc ttggcctgct cgttgatcac ccctgcgcgc tctgcaagtt ctccagcgcc
     1081 ttgttcaacg cgctgacact gctgttcaag ctccctagcg ctgcgctggt aaccttgttc
     1141 gagtgcgtta ccccgctctg caacgtccgg atctcctcgt gcagatcgct gcatagcttc
     1201 ccgcagctcc tgagcgcatc gctgcacacc ttttgcaacg cgatcaaaga gtgcgcctgc
     1261 tgctgcaatt gctgcctcaa gtcctcggtt tgttgttcgt gccgctgttc gatctgctcc
     1321 agcttcgcca acagtacccg ctccagctct gtcatttagc gcacctccac ggccatcttg
     1381 cccgcctctg tcttgtacgg cgtcccaatc ttgcgggcga tgaggtagcc cgtgccattc
     1441 ctgcccttga cgtagctcac ccccttcggc aactcgtcca gcgtgctctg agcgctgctg
     1501 atctcttgcc gcagcgtccg caacgtcccc atgtcttgct cgatctgatc ccatatccag
     1561 ctcatgccaa accaactccc caccagtaac cccaccccgc agaacatccc gagcagcgtt
     1621 gctttgaacc agacgctgga gaccagtttc agcgcttccc cttcgctccg ggcctgctcc
     1681 aagacctcct tcaatggttc ggtcgagtcg gtgatctgct ggggcaagtc ctcgatctcg
     1741 tccttgatag cggtcacggt tgtattgagc tcgccgctga tgcgcgtctg taagtcgcgc
     1801 ctgagcgtct ctagcagcgc ttgcgctaac ttctccacct cgcccaattc ctgctcttgc
     1861 cgctgcttga gcttctcgct caagcgtgct ttgtgctgtc caagactctc catacaactc
     1921 ccctttcaac cggatcgcct tcgggtatcc ctccggcttg acgctggtgt aatccttacc
     1981 tgtgcgcgtg atctcgcacc ctagcccctc ttgcaggtag gcccgcagtt cggcggcatt
     2041 gctcaccacc ccttgcgcga ctgcaccgat caggtggtcg gtgagctgct ttttgatggc
     2101 ggtgtggtct gtcacggcct tgtacgcttc aaaatcgggc cgcaacagcc gcgccctgag
     2161 cgggtcgtct ggccgcgccc accccttttc gtggttgtgc ttgtccctca ccgcatcgaa
     2221 gtctttgcgc tgccctggcg gggccggatt gaacgatttg ccgctgccca ggtgaacacg
     2281 ggggatcagg atatgcacat gcaccccgcc atccgcttcg gcgtgctcaa ccgcggtcaa
     2341 atgcacgttc tcccgcgcca gtccggcaaa tgccagcgcc tcgaagtcgt ccagcacagc
     2401 gctgatctcg tccggtcgcg gggcctcttc ggcagcccat gagatcactg cgctgctata
     2461 gcgctgctga aagggcagcg agtcggccac cgccgcgaac aacaccggat cccctcgcag
     2521 tactgccact cctgcccgtg gctcccctgt gtgatcgtgc ggccccatga gataagccga
     2581 ggcatgagcg ccgctgcccg tgccgtgggc aaggaattta atgtgcatcg ctctccccct
     2641 tgcggttcgg cttggggggc ggcggcaact ggggcagcag atgcgcggcc tgcccctcga
     2701 tgctgcgaag cagggccagc acctcgacca cttgcaccgg tgaaccgatc agccggcact
     2761 cgttcagggc gcgggcgatc tggttgaggt tgttgctcat ccctgccaat tgccgcataa
     2821 gtaccggatc cgcatcgtgg ctggtatctc gcactcggac cgcacgcttg cgcggggtcg
     2881 gcaggtgcgt ttgctgattg gcatcgatag cccccctgat ccagtccgac aaactgaggt
     2941 ctgacgcctc tgccttctcc cgccattcgg cggcggttgg cttgtctatc cgcacgctga
     3001 cggtggtcgt tctggccgtt ttggtcacgg tgcctccggt gttttttgtg tggggattga
     3061 aggggcgaag cccctcacca gccccgtgtt actgtgagcc gcgaagcggg cgaaacagta
     3121 atacgggtag gctggctaaa accaaggtag catgaaagta ggaggctgaa aaattggcaa
     3181 tttccgttac agtaacgcat aataaattat cgtcacagtg actaaaatgg agtgggtatg
     3241 aaggacagca ccgacaaggg aacgggggat atgtttggcg agaagcgtgg ccgagggaga
     3301 cccaaaaccg gaaccgccaa gacaggggca gaacgtcaag ccgcctttcg tgccaaacaa
     3361 caggataata gcgttacagt aacgattaat cgggaactgc tggatggact ggatgcccaa
     3421 atgcaggcga tcagggatcg cggttcggtc gtggtgctga caccggaaca agcgggcgag
     3481 atcttgaagg ccatacgcaa atcaagccta aagcagctcc gtggacaacg cccccgccca
     3541 caggccaaac ccacggcctg atggacggcc tacggttcgg tcacttgccc acctcgccaa
     3601 ggttctctct cgaatacctc gagatatgga aacgccccta acggggcgct gttatctgat
     3661 aaggctttta ttggatcact ctggatcacg tttgagatag cggctcaccg tagcggagcc
     3721 aattccaaga tcctcgccta tatcgcgatt gctgtagccc atagccttga gtctgatcac
     3781 ctcgggcagc aaatccgccc tagccttacc gcttttggtt ggccgcttgg ataccttgcc
     3841 accaataggc ccaccgatac gccccccttt agcccctcga tgtgcctgca cctggctaaa
     3901 tcctgcgggg gtgaatcgat tccataccca acgggcgata ctcttggccg tggccttgat
     3961 ctccgacaca ggcagtgggc cgccctgctc ccgtccaaac gcattagcgc tatcacaagc
     4021 cgctctcacc gcattggccc aagcctcgta cccgttaggc cgccaatacg ccctaacggc
     4081 gctataggcc cattcacgca cgttatcaaa caccgtgacg ttacggccca gtccgccgac
     4141 ctctttgcga ctgcggcgac tataggccgc caaatccgca tcagggcaga actccgccag
     4201 atagtcgagg ctaaacggct cgctgtgatg ccatatcgtc caccaatcgc tatgtacggg
     4261 gttttgggtg ataagccctg catatcccat atccgccccc aaccgcttcg ccagaacgtg
     4321 ttctatggcc gccagatagc gcaacggctt ttgcttggcc gcacaggtac gggccacaac
     4381 agccgccaga tagtacccat agtgagcgtg cccgttgcgg gcgttgaacg tgatccagtt
     4441 cggggctggt aatccggcat ccttccacgc ctcatagccg tctgggctgt ccacatcgaa
     4501 caccagacag gctacgtgcg gcggcgggtt gtgctggata tggcgatagg ccagcgcggt
     4561 cgcttgcggg cggatcagca gcccctgcgc cggatcatcg gtgcagtagg gacgacgggg
     4621 gagacgttcg acgaaagcag cggattgaag tgcaggtttt accatctaac actgcgctta
     4681 ttggcggtgc tggcttgttt ggtgtgatcc atgtggtata tttcacctat tgctttgcat
     4741 aagtccgacc ccgaatgctc tgtattcttc cgccaagttg agacagagat tcggggtttg
     4801 tctttctggg caatacgtta ccgcaatcaa gccaccacta gtacactttt tctaggacgc
     4861 ccgcccttgg aaccatttgc ccgagcagcc gcaactttgg caggggattt tgccatgccg
     4921 cccttggaac ccattcggga tgcagcccag ttagccgacc caaacaatcc ttccaatagt
     4981 gcaggcaggt aaagatctgc atccaactta ggaaaatgca cgcccaatcc tgacggcgat
     5041 atttctacat ccagcaagtc acttggtttg gccgcttcaa gcccttgcac caagtgagga
     5101 gcaaaggcca gctctaaccc tgaagacaga gcgatcacaa cccgcccgat cctccgatca
     5161 taacgcgcac tagtggcaac aggattacgc ttgcgttcct ctgagccgca ttgatttgcc
     5221 cgctcaatca tgtcatcggt tagttccatg aatatcctcc catttggtta tcaggtgggc
     5281 ttggttgttt tgtaactcag cccagatacg tcgaagcgat ttgctatcaa acccgtggtt
     5341 ttcccttagt tcaagttcac cagcaagatt gaaaaccgcc tcgcccccgc cccctataac
     5401 gtgcacatgt gccggacggt gatcgttggg atagatcacc actcgccagc ccccaattat
     5461 caacaccgta ggcatctaaa aacctaaccg ctttggttta tttaatacaa tactacacct
     5521 aaaagaacgt ttattgccaa tagagatcat gccgaaaccc aagacttccc catcaaacct
     5581 gaacccttat tgcgttctac agcgaacgcc atgcaccgga aacccagtcg ggtgtttcca
     5641 tagcgggaaa atctgccaaa acattctctg gatgagcact cgtccccccc cttctaatgc
     5701 gtttgacttt tccattcaaa cacagcaaca tacacataca catacacata cacatacaca
     5761 tacacatact gaggcgggaa tggctgggaa aatgtgtcca aaatgtggcg ttcaaacttt
     5821 tttcaaaacg ccaacaggaa gaaagtgctc aaaatgcgaa tatgaaatgc gcgttccagc
     5881 aaataatgga atgggggggc aaggctgtcg ttgc
//
