LOCUS       NZ_AP022250             3274 bp    DNA     circular CON 14-JUN-2023
DEFINITION  Escherichia coli strain WP8-S18-CRE-02 plasmid pWP8-S18-CRE-02_5,
            complete sequence.
ACCESSION   NZ_AP022250
VERSION     NZ_AP022250.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194621
            Sequence Read Archive: DRR199457
            Assembly: GCF_014169695.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Escherichia coli str. WP8-S18-CRE-02
            isolated from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 573x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/14/2023 05:11:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,006
            CDSs (total)                      :: 4,886
            Genes (coding)                    :: 4,642
            CDSs (with protein)               :: 4,642
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 244
            CDSs (without protein)            :: 244
            Pseudo Genes (ambiguous residues) :: 0 of 244
            Pseudo Genes (frameshifted)       :: 73 of 244
            Pseudo Genes (incomplete)         :: 167 of 244
            Pseudo Genes (internal stop)      :: 44 of 244
            Pseudo Genes (multiple problems)  :: 37 of 244
            Pseudo Genes (short protein)      :: 4 of 244
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022250.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="WP8-S18-CRE-02"
                     /isolation_source="wastewater"
                     /db_xref="taxon:562"
                     /plasmid="pWP8-S18-CRE-02_5"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6316 N 139.7460 E"
                     /collection_date="2018-08-06"
     gene            101..250
                     /locus_tag="H7R30_RS24670"
                     /old_locus_tag="WP8S18C02_P50010"
     CDS             101..250
                     /locus_tag="H7R30_RS24670"
                     /old_locus_tag="WP8S18C02_P50010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            1107..1940
                     /locus_tag="H7R30_RS24675"
                     /old_locus_tag="WP8S18C02_P50020"
     CDS             1107..1940
                     /locus_tag="H7R30_RS24675"
                     /old_locus_tag="WP8S18C02_P50020"
                     /inference="COORDINATES: protein motif:HMM:NF027299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_072775497.1"
                     /translation="MILLAGKKMKTNYFTHRLFPSDGAVICEPLKTSFDNMIATNRNTA
                     TLANEYHVIQHVSGDVYTFIKTNSRDVFRKLDTSSNICVDLSNILNQHEKIAFASFFIL
                     KGDVIGFSNTLYSPRVGKLAEIYDAAMYSRNGNHNINFEPITNIVTEQDVMNYAHVGKI
                     TMKIEKSQGIIGGLGTLFSGNVKYDDVDSFEIKIIPKRAKDIKDTFAGLMQARPQEVSS
                     VAVSAKEHIGDVATDLNVIMSNTVYDFVNPNDTTTIEVQMEKNYNNNTTLRSLGY"
                     /name="hypothetical protein"
     gene            1951..2388
                     /locus_tag="H7R30_RS24680"
                     /old_locus_tag="WP8S18C02_P50030"
     CDS             1951..2388
                     /locus_tag="H7R30_RS24680"
                     /old_locus_tag="WP8S18C02_P50030"
                     /inference="COORDINATES: protein motif:HMM:NF027298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_046597188.1"
                     /translation="MKLKGFWLSAIVIAACNGMGFLFLYLSKTYFFPMVTFKDNLGNLG
                     SSLVTYSTAMMALLIAMVVIIFGLDGPRLHNFKKSGYLHVTYFIYMITFFELGLTMALS
                     LLCLSNISSIKVASYSLTFAFITFLLIGCLGIQLLNIKKNL"
                     /name="hypothetical protein"
     Typing          complement(2706..3192)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.081"
                     /coverage="93.0"
                     /evalue="5.84e-177"
ORIGIN
        1 aacatattca gcctgtatac actccgctag cgctacgtga ctggttcagg gctgcgcccc
       61 gaaacccgct aacaacctct cagctactgg atatcacccc atgccatctg aatatgatga
      121 acaactggat tcgactgata cgcagcttcc cggcgaatat gacctttttg atttaatggc
      181 gcaggttact gaagagaacc tgcatgcccc tgtggatttt gggtcgcctg tcggccggga
      241 agcgttatag ctttttcgct tgcccgcgta cgtggtaata ctccggtatt accttttatt
      301 tctttatttt catgtaatta cagtggattt ttaatacccg ggtattactc gtgaaaacga
      361 aatattcctt ttttttcttt taattcatgc agttgcgatg aaaatcgcgg ctgcgttagg
      421 tgtatgacca ttttgaggtt gaaaaataat caaccatggc gggtgattta cgcaggttaa
      481 ggggagatcg tgacaaaaaa gcgttcctca ctgtatcgtt catgcctgca tgaacgggtt
      541 attcttcctg actacagaat tacatcaaaa gtacatcaaa attacatcac ttttacatca
      601 cttttacatc acttttacat cattccttca gctcatacgt cactttcatc ctctgtgcca
      661 tttgtacatc acccccttcc tgaacgtgct gacagcgggt tttccggctg cgccggcaac
      721 gcttcacggc cacgcaataa attgcgtccc cgttcaccgt tttcggatgg gaagaacgcc
      781 ggaaacagga cttaccccgg ctgaatggtg tgaatttccg cgctatgcac ttgtgcgcat
      841 actcatgcat gccgtaaaaa cagagcctgc gcgtttctgg cgggttttcg ggtggtttgt
      901 tgcctgtttt accggtttcc cgtcagaaac gcgctgaggg cctctgcggc ggtgcacgca
      961 acggatgtta tggtaaatag agttggtggc ctagctttac gaccttcggt gacagatcat
     1021 ttaatatgca aaacttggaa ttttaaagaa gcattgttct tccataagaa atagaatata
     1081 aaaagaagaa aatataatgc gtttctatga ttttactagc tgggaaaaaa atgaaaacaa
     1141 attacttcac acacagattg tttccttcag atggcgctgt tatttgtgag ccattaaaaa
     1201 ctagttttga taatatgatc gctactaaca gaaacactgc aacgctagcc aatgagtatc
     1261 atgtaattca gcatgtgtct ggcgatgtat atacgtttat aaaaacaaat agcagagacg
     1321 tttttagaaa actagatacg agtagtaata tatgtgtaga tctctcaaac atcttaaatc
     1381 aacatgaaaa aatagccttt gcatcttttt tcatattgaa gggtgatgtt atagggttta
     1441 gtaatacgtt gtactctcct cgtgttggga agttggcaga aatatatgat gctgcaatgt
     1501 attcccgaaa tgggaaccac aacataaatt ttgagccgat aacaaacata gttaccgagc
     1561 aagatgttat gaattatgcc catgttggta aaataacgat gaaaatagag aagtctcaag
     1621 gcattattgg tgggcttggt acgcttttct ctgggaacgt aaagtatgac gatgtggatt
     1681 cgtttgagat aaagataatc cctaaacggg ctaaagacat aaaggatacc tttgcaggtt
     1741 tgatgcaggc taggcctcaa gaggttagct cggtcgctgt gtcagctaaa gaacacattg
     1801 gtgacgttgc aacagatttg aatgttataa tgtctaatac cgtatatgat tttgtaaatc
     1861 caaatgacac tactactatc gaggttcaga tggaaaaaaa ttataataac aatacaacat
     1921 taagaagttt aggttactga ggattgcatt gtgaagctta agggtttttg gttgagcgct
     1981 atagttatag ctgcatgtaa tggcatgggc tttttatttc tatacctttc aaaaacctat
     2041 ttcttcccga tggtaacgtt taaggataat ttaggtaacc ttggttcttc cttggttact
     2101 tattcgacag cgatgatggc gttgcttatt gctatggttg ttataatctt tggcttggat
     2161 ggacctcgct tgcataactt taaaaaaagc ggctatctcc atgttacata ttttatttat
     2221 atgataacgt ttttcgagct cggcttaacc atggctcttt cgttactttg tttgtccaat
     2281 atctcaagta ttaaggtggc ttcttattca ttgacttttg catttattac ttttttgttg
     2341 ataggatgtt taggtattca actcttaaat ataaaaaaga atctttaatc aaccaaactg
     2401 tgatgttcat cacaggtgaa acggtttgtg accatttttg gtgagttgat aaaatgcaga
     2461 atgtgtaagg atcgtcttga gatttttgag agtgcagcgt agtctcttgc tctgtaaaca
     2521 aaaaaaccac cgctaccaac ggtggtttgt ttgccggaag tgagagctac caactctttc
     2581 tcccgaaggt aactggcttt cacggagcgc agataccaaa actgtccttt tagtgtagcc
     2641 gtagtcaggc caccacttca agaactccat agaaatactc tactactctg ctccgtgaat
     2701 cctcttacca atggctgctg ccaatggcgc gttgtcgtgt ctttccgggt tggactcaag
     2761 aagacagtta ccggaaaggc gcagtagtcg gactgaacgg ggggttcgtg catacagtcc
     2821 agcttggagc gaactgccta cccggaactg agtgtcaggc gtggaatgag ataaacgcgg
     2881 ccataacagc ggaatgacac cggtaaaccg aaaggcagga acaggagagc gcacgaggga
     2941 gctaccaggg ggaaacgcct gggatcttta tagtcctgtc gggtttcgcc gccactgatt
     3001 tgagcgtcag attttgtgat gcttgtcagg ggggcggagc ctatggaaaa acggcagcgc
     3061 cgtatccttt ttccggcctt tcctgacttg agagccatct ttttatcttg tcattcgtga
     3121 gcaggtaacg cccgccgcag tctcacggca ggagcgtagc gaccgagtga gcgaggaggc
     3181 gtcatgtcac cgttacctgc attttgcact tacgtcctat agccgcattt tcccttgcaa
     3241 catgaataac ttccctgaat taccactccg acct
//
