LOCUS       NZ_AP022194             4901 bp    DNA     circular CON 02-DEC-2023
DEFINITION  Klebsiella sp. WP7-S18-CRE-02 plasmid pWP7-S18-CRE-02_5, complete
            sequence.
ACCESSION   NZ_AP022194
VERSION     NZ_AP022194.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194568
            Sequence Read Archive: DRR199404
            Assembly: GCF_014169515.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella sp. WP7-S18-CRE-02
  ORGANISM  Klebsiella sp. WP7-S18-CRE-02
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Klebsiella sp. WP7-S18-CRE-02 isolated
            from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4901)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 239x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/02/2023 11:36:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,143
            CDSs (total)                      :: 5,025
            Genes (coding)                    :: 4,879
            CDSs (with protein)               :: 4,879
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 146
            CDSs (without protein)            :: 146
            Pseudo Genes (ambiguous residues) :: 0 of 146
            Pseudo Genes (frameshifted)       :: 44 of 146
            Pseudo Genes (incomplete)         :: 101 of 146
            Pseudo Genes (internal stop)      :: 20 of 146
            Pseudo Genes (multiple problems)  :: 16 of 146
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022194.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Klebsiella sp. WP7-S18-CRE-02"
                     /mol_type="genomic DNA"
                     /strain="WP7-S18-CRE-02"
                     /isolation_source="wastewater"
                     /db_xref="taxon:2675716"
                     /plasmid="pWP7-S18-CRE-02_5"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.5619 N 139.7531 E"
                     /collection_date="2018-08-06"
     gene            complement(101..403)
                     /locus_tag="H7R68_RS25630"
                     /old_locus_tag="WP7S18C02_P50010"
     CDS             complement(101..403)
                     /locus_tag="H7R68_RS25630"
                     /old_locus_tag="WP7S18C02_P50010"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_182930714.1"
                     /translation="MRRTPEQRKAYLEAQLNSVKAKINKQKRKDYTVKKRETDHLKYTF
                     AGDILKVMGCDVREVDLALVLGAVYFATSENANPKNVELYKRKGLEILKENKKEI"
                     /name="hypothetical protein"
     gene            complement(400..942)
                     /locus_tag="H7R68_RS25635"
                     /old_locus_tag="WP7S18C02_P50020"
     CDS             complement(400..942)
                     /locus_tag="H7R68_RS25635"
                     /old_locus_tag="WP7S18C02_P50020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_182930715.1"
                     /translation="MVRRKITVSEKTKALFKPFFDVSSYKEKKEGFEEEEYKRLVGLCF
                     ESMGKGKLSINRPMATGKGILTFIYRTDKDLLLDEFIDRFEKKITKKRYMDFLLFILVD
                     DTKNIEKIEKMFTRICGVDIEDNVLKAIKCLKADGYDYKGFGDLIDYFEGLKRVRLEKE
                     ELNKIMASSKNKKRGRL"
                     /name="hypothetical protein"
     gene            1205..2482
                     /gene="mobQ"
                     /locus_tag="H7R68_RS25640"
                     /old_locus_tag="WP7S18C02_P50030"
     CDS             1205..2482
                     /gene="mobQ"
                     /locus_tag="H7R68_RS25640"
                     /old_locus_tag="WP7S18C02_P50030"
                     /inference="COORDINATES: protein motif:HMM:NF041496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_182930716.1"
                     /translation="MAIYYFDVSNVSRSSGKKSASSVAASAYQNRARYTDERTGTTYDY
                     TKKGGLYYSGILAPENAPESLTASPLTLWNTIEGIEKRKDARLAKDFKISLPIELSPEQ
                     NKALILDFAKQAFVSKGMIVDLAIHDIDTQPHSHIKTTTRELTPEGFGGKVREWDKKET
                     TLYWRELWAKVANEHLLKAGHDITISHLTLKEQMKEALEEMDKTTDLEEKIKLASKAIE
                     LNRPAMKTISKKDWGDPKFQEIRKQEQEAKKQILKKAKNFRIKNIRELREIQKELDKKF
                     RPSKIYKSISEAIRNLNNKITDSKLNNILNDKLDKLTKAIISIPENRKIKREKKIREKE
                     LEEEKRIFRETHKMDELGNIYKKGDSKKWNKENNKLIKQQTASNKAKAEEERERKKLED
                     EANRLAEKNNISPNDFKQKKKKGLSV"
                     /name="mobQ"
     gene            complement(2653..4446)
                     /locus_tag="H7R68_RS25645"
     CDS             complement(2653..4446)
                     /locus_tag="H7R68_RS25645"
                     /inference="COORDINATES: protein motif:HMM:NF013603.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_182930713.1"
                     /translation="MLKNFRTKNNQFPGNGTPLQKPPQNTPKNGTEHLDHYPTNGRFGT
                     EHFKPCFFGVKNGTVPTVTAKKHGKNGTVSTFSRWEKIRGGSDKCLTDLTKRKNPVKWN
                     LRRFKQYGVFLNMFTGTSLINKSRSVNRPDNLKEFASVNGERITERKKETFNIALSMKD
                     KGEDLQDETLTKRAERSLKCSSFRYIATVNGQAQTLYTHRCKGRHCQECQRIKAYIWQK
                     KNEAIFPKLEENYPNAKYLFATFTIKNPKITDLRVVLSVMNKAANRLFKMKKYKDFILG
                     GIRSTEITRGKSGADECHPHFHFLLMVKGNYGKKYYVNQEDWGKDWGDCLAYECDRAGY
                     PYNPIDYPRGFPIVDVVRAMDKERKIEITNKNIKTCGEAIINYVLKYSVKGSDILDHKK
                     GKNDDWFFEFDKQIKGCRMITPVGVFKKYLSEIKKDPFDYEIEKAKIEERLGEGYQVNK
                     AIFKKGDYKLENKKDSEGYLLEALEAKAYNFQQLYLTIYSDYIKFYNDTKREISKLEDK
                     LFNEDNMLIESKIGRLLLIQEDRKKRINNTIESLKRVGYLKEEAGLYYDEWGSLWATFE
                     IEKPNIEIVEINDFADMFEVW"
                     /name="protein rep"
ORIGIN
        1 agccgtatat atattaataa ataatttctt aactctccac aggaaaggat actaaatata
       61 taaacgacta tatattaaat atattctaat tatgtttttg tcaaatttct tttttatttt
      121 cttttaaaat ttccaacccc tttcttttat ataactctac atttttaggg tttgcatttt
      181 cacttgtagc aaagtaaaca gcaccaagca ccaaagcaag atcaacttcc cgaacgtcgc
      241 accccataac ttttaaaata tcacctgcaa aagtatattt tagatgatcc gtttctcttt
      301 tcttaacagt gtaatctttt cttttctgct tgtttatctt tgcttttaca ctattaagct
      361 gagcttccaa atatgctttc ctctgttctg gtgttcttct cataatctcc ccctcttttt
      421 atttttactt gaagccataa ttttattaag ttcctctttt tctaatctaa ccctttttaa
      481 accctcaaaa taatcaatta aatcaccaaa ccctttataa tcataaccgt cagcctttaa
      541 acatttaata gcctttaaaa cattatcttc aatatctaca ccacaaattc gagtaaacat
      601 tttctctatt ttttctatgt ttttagtatc gtcaaccaaa ataaaaagca aaaaatccat
      661 atatcttttt ttagttattt tcttctcaaa tctatctata aactcgtcta acaataaatc
      721 tttatcagtc ctatatataa aagttaaaat tcctttaccg gttgccattg gtctattaat
      781 gctgagtttt ccttttccca tactttcaaa acacaaccca acgagtcttt tatattcttc
      841 ttcttcgaag ccttcttttt tttctttgta tgaactcacg tcaaaaaaag gtttaaataa
      901 tgcctttgtt ttttctgata ctgttatttt tctccttacc ataaaaacct ccattatatt
      961 tagattaatt aataaccata tacctccgta atttacttta gtcaaattta tgttatatct
     1021 aaaataagga aaagcatttt tgccgttctg aaagtttttt attttgagtt tacgaaaaaa
     1081 taaaattact tgaagccctt ctacgcattg cctttggcaa tgacagggtt tttccccgta
     1141 aacgggaaaa aagaaaagca aaaaccaaac cccaaaaaaa ttagaaaagt ggaggtttta
     1201 caatatggct atatattatt ttgatgtttc caatgtaagc agaagttcag gcaaaaaatc
     1261 ggcttcgtct gttgctgctt ctgcatacca gaacagagcc agatacacag acgaacgaac
     1321 aggcacaacc tacgactaca caaagaaggg ggggttatac tattcaggca ttttagcccc
     1381 tgagaatgcc cctgagagcc ttacagcttc accgttgacc ctatggaaca ccatagaggg
     1441 tattgagaaa agaaaggatg ccaggcttgc caaagacttt aaaatttccc tacccataga
     1501 gctatcccca gaacagaaca aagccttaat tttagacttt gcaaaacagg cttttgtatc
     1561 aaagggtatg attgtagatc tggctattca cgatatagac acccaacctc atagccatat
     1621 taaaacaact accagagaat taacaccgga gggatttggt ggaaaagtta gggagtggga
     1681 taaaaaagaa acgactttat actggaggga attatgggca aaagtggcga acgaacattt
     1741 attaaaggct gggcatgata taaccattag ccatttaaca ttaaaagaac aaatgaaaga
     1801 agcactggag gaaatggaca aaacaacaga cctggaggaa aaaataaaac ttgcttcaaa
     1861 ggctatcgaa ttaaatagac cagcaatgaa aacaatcagc aaaaaagact ggggcgatcc
     1921 caaatttcag gaaataagaa aacaggaaca ggaggcaaaa aaacaaatat taaaaaaggc
     1981 taaaaatttt agaattaaaa acataagaga attaagagaa atacaaaaag aattggataa
     2041 aaaatttaga ccgtcaaaaa tatataaatc tatttccgaa gcgattagaa atttaaacaa
     2101 taaaatcacc gactcaaaat taaataatat cctaaatgac aaattagata aactaacaaa
     2161 agccattata tcaataccag aaaatagaaa aattaagagg gagaaaaaaa taagagagaa
     2221 agaattagag gaagaaaaaa gaatattcag agaaactcat aaaatggatg agctcggaaa
     2281 tatttataaa aaaggagact ccaaaaaatg gaataaggaa aataataaac ttattaaaca
     2341 gcaaacagcc agtaataaag caaaagccga agaagaaaga gagaggaaaa aattagagga
     2401 cgaggctaat agattagctg agaaaaataa tatttcacct aatgacttta agcaaaagaa
     2461 aaagaaaggg ttaagtgtat aggactatat actactatat actatactac tctttcttta
     2521 aaattgtgat ccttgctggt tcaatgtttc ctttcgaaat ttcgacggct tcacgaacgg
     2581 aagctaagag cattttaaaa aatttgctct ttctgttccg atttcttttt ttaggtttct
     2641 gcttcttttt cattaccaca cctcaaacat atcagcaaaa tcattaattt ccactatttc
     2701 aatattaggt ttttcaattt cgaatgttgc ccaaagagat ccccattcgt cataatataa
     2761 cccagcttct tctttaagat aaccgaccct ttttaaactt tcaattgtat tgttaattcg
     2821 ttttttcctg tcttcctgaa ttaataataa tctacctatt ttagactcga tcaacatatt
     2881 atcttcatta aataacttat cttctaattt agatatttcc cttttagtgt cattatagaa
     2941 cttaatataa tcactatata tagttaaata taattgctgg aaattatagg cttttgcttc
     3001 caatgcctcc aataaataac cctcactatc ttttttattt tcaagcttat aatcgccttt
     3061 tttaaatata gctttattaa cctgataacc ttcccccaat ctttcttcta tttttgcttt
     3121 ttcaatctca taatcaaacg gatctttttt aatttcgctt agatattttt taaaaactcc
     3181 aacaggtgta atcattcgac aaccttttat ttgcttatcg aattcaaaaa accaatcgtc
     3241 atttttacct tttttatgat ctaaaatatc actacccttg acggaatatt ttagaacata
     3301 attaataatg gcttcaccac aagtttttat attcttattt gtaatttcaa ttttcctttc
     3361 tttatccata gccctaacga cgtccacgat tgggaaccca cgaggatagt cgattgggtt
     3421 atacggatac cctgccctat cacactcata agccaggcaa tccccccaat ctttccccca
     3481 atcttcttga tttacatagt attttttacc gtaattgcct tttaccataa gcaaaaaatg
     3541 aaagtgggga tggcattcgt ctgccccaga ttttcccctt gtgatctccg tcgaacggat
     3601 acctccaaga ataaaatctt tatatttttt cattttaaaa agtctatttg ctgctttatt
     3661 cattacagat aaaacaaccc ttagatctgt tatttttggg tttttaatcg taaatgtcgc
     3721 aaataaatat tttgcatttg gatagttttc ttccagtttt ggaaaaatag cctcattttt
     3781 tttctgccag atataagcct taattctttg gcattcctgg caatggcgac ctttgcaacg
     3841 gtgagtatat aaggtttgag cctgtccgtt aacagtagcg atatagcgaa aagaagaaca
     3901 ctttaaagac ctttcagccc ttttagttaa tgtctcgtct tgtagatctt cgcctttgtc
     3961 tttcatagac aaagcaatat taaaggtctc tttttttctt tctgttattc gttcaccgtt
     4021 tacactggca aactctttta gattatccgg tctattgaca gacctacttt tattaattag
     4081 acttgtccct gtaaacatat taagaaatac cccgtattgt ttgaatcttc gcaaattcca
     4141 ctttacgggg ttttttcttt ttgtcaaatc agtcaagcac ttatcagatc cccctctaat
     4201 tttctcccac cggctaaaag tcgataccgt cccatttttt ccgtgttttt ttgccgttac
     4261 ggttggaacc gtcccatttt tcaccccaaa aaagcagggt ttaaaatgct ccgtcccaaa
     4321 tctgccgttt gtcggatagt gatctaaatg ttccgtccca ttttttgggg tgttttgggg
     4381 aggtttctgc agtggtgtcc catttccggg gaattgattg ttttttgttc taaaattctt
     4441 tagcaaaaag tgctatttac cccccttaaa aaacaccaaa aaaagggggt taaagccagt
     4501 aatggcgggg cttgcagggg agttttagag gctaagtttt gcctccattt gtttcttact
     4561 aaacaagggg aggggtcgcc ttcggcaacc ttcaaaccaa acccctcaaa ccaaaggcaa
     4621 ccactccgtg gcaatggtat aggcatataa cttagttatg caatcctgga cgaatgaaca
     4681 acatttaaag acggcttaac aggtggaagg ctctctttgt tcaccttcca cctttgccaa
     4741 gtgttgtatt gaccagggag ccgtacaagt acaaaatcgg aacccttaaa agggaacccc
     4801 cgattttctc cttgacggtt cggcactggt gaacggtatc agaaaggacg aaaggctaac
     4861 ctaagttagc ctctccaata tccccataaa tggggcaaat t
//
