LOCUS       NZ_AP022165             6647 bp    DNA     circular CON 14-JUN-2023
DEFINITION  Escherichia coli strain WP5-S18-ESBL-08 plasmid pWP5-S18-ESBL-08_4,
            complete sequence.
ACCESSION   NZ_AP022165
VERSION     NZ_AP022165.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194534
            Sequence Read Archive: DRR199370
            Assembly: GCF_014169415.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Escherichia coli str. WP5-S18-ESBL-08
            isolated from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 302x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/14/2023 05:34:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,406
            CDSs (total)                      :: 5,284
            Genes (coding)                    :: 5,080
            CDSs (with protein)               :: 5,080
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 204
            CDSs (without protein)            :: 204
            Pseudo Genes (ambiguous residues) :: 0 of 204
            Pseudo Genes (frameshifted)       :: 75 of 204
            Pseudo Genes (incomplete)         :: 137 of 204
            Pseudo Genes (internal stop)      :: 38 of 204
            Pseudo Genes (multiple problems)  :: 42 of 204
            Pseudo Genes (short protein)      :: 5 of 204
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022165.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="WP5-S18-ESBL-08"
                     /isolation_source="wastewater"
                     /db_xref="taxon:562"
                     /plasmid="pWP5-S18-ESBL-08_4"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6538 N 139.5126 E"
                     /collection_date="2018-08-06"
     gene            101..538
                     /locus_tag="H7R36_RS26565"
                     /old_locus_tag="WP5S18E08_P40010"
     CDS             101..538
                     /locus_tag="H7R36_RS26565"
                     /old_locus_tag="WP5S18E08_P40010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            619..1038
                     /locus_tag="H7R36_RS26570"
                     /old_locus_tag="WP5S18E08_P40020"
     CDS             619..1038
                     /locus_tag="H7R36_RS26570"
                     /old_locus_tag="WP5S18E08_P40020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(1356..1589)
                     /locus_tag="H7R36_RS26575"
     CDS             complement(1356..1589)
                     /locus_tag="H7R36_RS26575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1596..2114)
                     /locus_tag="H7R36_RS26580"
     CDS             complement(1596..2114)
                     /locus_tag="H7R36_RS26580"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2152..2583)
                     /locus_tag="H7R36_RS27160"
                     /pseudo=""
     CDS             complement(<2152..2583)
                     /locus_tag="H7R36_RS27160"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2792..3115)
                     /locus_tag="H7R36_RS26590"
                     /old_locus_tag="WP5S18E08_P40040"
     CDS             complement(2792..3115)
                     /locus_tag="H7R36_RS26590"
                     /old_locus_tag="WP5S18E08_P40040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3179..3370
                     /locus_tag="H7R36_RS26595"
                     /old_locus_tag="WP5S18E08_P40050"
     CDS             3179..3370
                     /locus_tag="H7R36_RS26595"
                     /old_locus_tag="WP5S18E08_P40050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4696..4923
                     /locus_tag="H7R36_RS26605"
                     /old_locus_tag="WP5S18E08_P40070"
     CDS             4696..4923
                     /locus_tag="H7R36_RS26605"
                     /old_locus_tag="WP5S18E08_P40070"
                     /inference="COORDINATES: protein motif:HMM:NF015489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_230498631.1"
                     /translation="MLYAIVISTILCPYSKYAIEHIAFNFIKKDFFERRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            complement(4920..6488)
                     /gene="cea"
                     /locus_tag="H7R36_RS26610"
                     /old_locus_tag="WP5S18E08_P40080"
     CDS             complement(4920..6488)
                     /gene="cea"
                     /locus_tag="H7R36_RS26610"
                     /old_locus_tag="WP5S18E08_P40080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     Typing          complement(3456..3543)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          3554..4112
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.544"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1253..2802)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acaccacgcg ctattctcgc ccgatgccac aaaaaccagc acaaacatta ccgttctcag
       61 acctcattat gttttactga aactatgaga tgagacatct atgggacact gtcactttat
      121 ggcatggcac acactccggg acgcactaaa aatgacaggc agatcgcgtt cacagtttta
      181 ccgtgatatg cgcgcaggcc ttgtcagtta ccgtaccggc agggacggac gacgggagtt
      241 tgaaaccagt gaactgatcc gggcatacgg cgaattaaag cagaatgaga caccagaaag
      301 gcacagtgag ggacatgcag aaaatccaca tgatcagcag acagaacgca ttctccggga
      361 gctgaatgag ctgaaacaat gcctgacgct gatgcttgag gataaacagg cacaggatat
      421 ggatcgcaga cgccaggaag cagaacggga acagctacaa aatgagatag cccagctcag
      481 gcaggcactg gaactggaaa agaaacgggg attctggtcc aggttgttcg gtcgctgaac
      541 gctgtcagag actgatgata aaatagtctt cggataataa ctcaccgaga ataaatactt
      601 taaggtaggg agacactcat gagacgtacc ggaaacaaac tttgtcttat cgccatgata
      661 acagcaacag tagctctcac agcctgtacc ccaaagggca gcgtggaaca acatacccgg
      721 cattacgtat atgcttctga tgacggtttt gatcccaact tttccaccca aaaagccgac
      781 acaacacgaa tgatggtgcc tttttttcgg cagttctggg atatgggagc taaagataaa
      841 gcgacaggaa aatcacggag tgatgtgcaa caacgcattc agcagtttca cagccaggaa
      901 tttttaaact cactccgggg cacaactcaa tttgcgggta ctgattaccg cagcaaagac
      961 cttaccccga aaaaatccag gctgctggct gacacgattt ctgcggttta tctcgatggc
     1021 tacgagggca gacagtaagt ggatttacca taatccctta attgtacgca ccgctaaaac
     1081 gcgttcagcg cgatcacggc agcagacagg taaaaatggc aacaaaccac ccgaaaaact
     1141 gccgcgatcg cgcctgataa attttaaccg tatgaatacc tatgcaacca gagggtacag
     1201 gccacattac ccccacttaa tccactgaag ctgccatttt tcatggtttc accatcccag
     1261 cgaagggcca tccagcgtgc gttcctgtat ttccggctga cgctcccgtt ccagggataa
     1321 cacatgttcg cgctcctgta tcagccgttc ctctcttatc tccagttctc gctgtataac
     1381 tggctcaagc gttctgtctg ctcgctcaag tgctgcacct gctgactcaa ctgcatgacc
     1441 cgctcgttca gcatcgcgtt gtcccgttgc gtaagcgaaa acatcttctg caattccacg
     1501 aaggcgctct cccattcgct cagccgctgc atatagtcct gttgcagctg ctctaaggcg
     1561 ttcagcaaat gtgtttccag ctctgtcact ctgtgtcact ccttcagatg tacccactct
     1621 ttcccctgaa agggaatcac ctccgctgat ttcccgtacg gaaggacaag gaatttcctg
     1681 ttcccgtcct gcacaaactc cacgccccat gtcttcgcgt tcagtttctg caatgtctct
     1741 tcctgcttcc tgatttcttc caggttcgcc tgtatcctcc ctccaagata ccagagcgtc
     1801 ccgccactcg cggtaaacag gagaaagact atccccagta acatcatgcc cgtattccct
     1861 gccagcttta acacgtccct cctgtgctgc atcatcgcct ctttcacccc ttcccggtgt
     1921 ttttccagcg attcctctgt cgaggctgtg aacagggcta tagcgtctct gattttcgtc
     1981 tcgtttgatg tcacagcctc gcttacagat tcgccgagcc tcctgaactc gttgttcagc
     2041 attttctctg tagattcggc tctctctttc agctttttct cgaactccgc gcccgtctgc
     2101 aaaagattgc tcataaaatg ctcctttcag cctgatattc ttcccgccgt tcggatctgc
     2161 aatgctgata ctgcttcgcg tcaccctgac cacttccagc cccgcctcag tgagcgcctg
     2221 aatcacatcc tgacggcctt ttatctctcc ggcatggtaa agtgcatcta tacctcgcgt
     2281 gacgccctca gcaagcgcct gtttcgtttc aggcaggtta tcagggagtg tcagcgtcct
     2341 gcggttctcc ggggcgttcg ggtcatgcag cccgtaatgg tgattcacca gcgtctgcca
     2401 ggcatcaatt ctaggcctgt ctgcgcggtc gtagtacggc tggaggcgtt ttccggtctg
     2461 tagctccatg ttcggaatga caaaattcag ctcaagccgt cccttgtcct ggtgctccac
     2521 ccacaggatg ctgtactgat ttttttcgag accgggcatc agtacacgct caaagctcgc
     2581 catcactttt tcacgtcctc ccggcggcag ctccttctcc gcgaacgaca gaacaccgga
     2641 cgtgtatttc ttcgcaaatg gcgtggcatc gatgagttcc cggacttctt ccggattacc
     2701 ctgaagcacc gttgcgcctt cgcggttacg ctccctcccc agcaggtaat caaccggacc
     2761 actgccacca ccttttcccc tggcatgaaa tttaactatc atcccgcgcc ccctgttccc
     2821 tgacggccag acgcagccgg cgcagctcat ccccgatggc catcagtgcg gccaccacct
     2881 gaacccggtc accggaagac cactgcccgc tgttcacctt acgggctgtc tgattcaggt
     2941 tatttccgat ggcggccagc tgacgcagta acggcggtgc cagtgtcggc agttttccgg
     3001 aacgggcaac cggctccccc aggcagaccc gccgcatcca taccgccagt tgtttaccct
     3061 cacagcgttc aagtaaccgg gcatgttcat catcagtaac ccgtattgtg agcatcctct
     3121 cgcgtttcat cggtatcatt accccatgaa cagaaatccc ccttacacgg aggcatcagt
     3181 gactaaacag gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc agacattaac
     3241 gctgctggag aagctcaacg aactggacgc agatgaacag gccgatattt gtgaatcgct
     3301 tcacgaccac gccgatgagc tttaccgcag ctgcctcgca cgtttcgggg atgacggtga
     3361 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtgag cggatgccgg
     3421 gagctgacaa gcccgtcagg gcgcgtcagc aggttttagc gggtgtcggg gcgcagccct
     3481 gacccagtca cgtagcgata gcggagtgta tactggctta accatgcggc atcagtgcgg
     3541 attgtatgaa aagtacgcca tgccgggtgt gaaatgccgc acagatgcgt aaggagaaaa
     3601 tgcacgtcca ggcgcttttc cgcttcctcg ctcactgact cgctacgctc ggtcgttcga
     3661 ctgcggcgag cggtactgac tcacacaaaa acggtaacac agttatccac agaatcaggg
     3721 gataaggccg gaaagaacat gtgagcaaaa gaccagaaac aggaagaagg ccgcgtagca
     3781 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     3841 gaggtggcga aacccgacag gactataaag acaccaggcg tttccccctg gaagctccct
     3901 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     3961 gggaagcgtg gcgctttctc atagctcacg ctgttggtat ctcagttcgg tgtaggtcgt
     4021 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     4081 cggtaactat cgtcttgagt ccaacccggt aaggcacgcc ttaacgccac tggcagcagc
     4141 cactggtaac cggattagca gagcgatgat ggcacaaacg gtgctacaga gttcttgaag
     4201 tagtggcccg actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     4261 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgttggt
     4321 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     4381 gatcctttga tcttttctac tgaaccgcga tccccgtcag tttagaagag gaggatggtg
     4441 cgatggtccc tccctgaaca tcacgtatat agttagcctg acatccaaca aggaggttta
     4501 tcgcgaatat tcccacaaaa aatcttttcc tcataactcg atccttataa aatgaaaaga
     4561 atatatggcg aggtttaatt tatgagctta agatactaca taaaaaatat tttatttggc
     4621 ctgtactgca cacttatata tatatacctt ataacaaaaa acagcgaggg gtattatttc
     4681 cttgtgtcag ataagatgct atatgcaata gtgataagca ctattctatg tccatattca
     4741 aaatatgcta ttgaacacat agcttttaac ttcataaaga aagatttttt cgaaagaaga
     4801 aaaaacctaa ataacgcccc cgtagcaaaa ttaaacctat ttatgctata taatctactt
     4861 tgtttggtcc tagcaatccc atttggattg ctaggacttt ttatatcaat aaagaataat
     4921 taaatcccta acacctcatt tatagtatta agtttattct tatcaatata ggagcataga
     4981 attcctgtaa caatagcaat accccaaata cctaatgtag ttccagcaag caagctaaaa
     5041 agtaaagcaa caacataact cacccctgca tctgcagctt tcttctctaa tgtaagaaat
     5101 agtggcttcc agtcacctgt atccttaatt tttaggatat cagataccac atcatatcca
     5161 aaagaaacat gccccgtaat cttcaagtac ttggcaaact gatctaaatg tttagcccag
     5221 tcatcatact tcaccgatgc caacgcatta aaaatagcat cacgatcggc tttgctgaat
     5281 ttcttattta aaacatcctt gtatttttca aaagcagcga gagcttcatt cacattgccg
     5341 attttcttac ctttagactt atcagcaagt tcctgtgcca ttttcgaata tttttcacca
     5401 tatttttcag tcagcgtttg ataaaagcta actgttgcat caacagcatc cttaatctgt
     5461 gaattaagga gattattctg tgcttttttc aaattttctt cagcttcatg aacacgagcg
     5521 ataccggcat tacgattatt actgacctga gaaatagcct tctggatctg agttatatca
     5581 gcatttatcc ggttaatacg tgtttctgat gctgttacct gtttttgttt ttcttctcta
     5641 atcttaccgg ccccaacccg tcgtctggtt gcttcaaaaa aaggacggtt ctgaagcgga
     5701 tcattggctc ttggtgatag ttttttgacc agctcatcca gttctttata tttagcggat
     5761 gcctgagcca gttcatttcg ttttccagcg agcgttttca tttctgcatc acgggcatgg
     5821 atactggagc ttaaacgaga attgagagtc ttaatctctc catccatttt caccacttca
     5881 gattgtgcag cagaaagttt tttttgggcg atctcaacag ctttagcttc ttcactcaat
     5941 gcagccagtc gtttctcttc agcttcagcc agtttcaact ggcgttctgt ttcagccttc
     6001 tcccgttcaa tctctttacg tcgttgttct gcttcctgaa aagccttttc tgctgcttcc
     6061 gcttctttac gggctttttc ttctgctttc gcaaggcgca aacgctctgc ttccgcctgc
     6121 atagctgcat tattagcatg agcaagctct gttgctgaag gcgtacgtga ggcattgtga
     6181 cgaagagcct cattcacgat atccttcagg cgctgagtca gcgcatccct gtttgccttt
     6241 gctttcgcct gtgcttccgc tgcagctttt gcccgggcag cctgctctgc ctgtgttttc
     6301 tttaattgag cagtagacca tttagcagtt gcatgaatag ctgcagaact ttcactttta
     6361 ctgcctcctt ttccacctcc gccgccagag ccactcccgt caggagtacc attcaaaaga
     6421 gtaataatta cctgtccctt atcatcataa ggaacaccat ctttatagta cgctaccgcg
     6481 gtttccatta taaaatcctc tttgactttt aaaacaataa gttaaaaata aatactgtac
     6541 atataaccac tggttttata tacagcataa aagctacgcc gctgcatttt ccctgtcaag
     6601 actgtggact tccatttttg tgaaaacgat caaaaaaaca gtctttc
//
