LOCUS       NZ_AP022156             2203 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella quasipneumoniae strain WP5-S18-ESBL-05 plasmid
            pWP5-S18-ESBL-05_14, complete sequence.
ACCESSION   NZ_AP022156
VERSION     NZ_AP022156.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194531
            Sequence Read Archive: DRR199367
            Assembly: GCF_014169355.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Klebsiella quasipneumoniae str.
            WP5-S18-ESBL-05 isolated from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 195x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 10:17:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,866
            Genes (coding)                    :: 5,626
            CDSs (with protein)               :: 5,626
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 79 of 240
            Pseudo Genes (incomplete)         :: 174 of 240
            Pseudo Genes (internal stop)      :: 45 of 240
            Pseudo Genes (multiple problems)  :: 48 of 240
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022156.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WP5-S18-ESBL-05"
                     /isolation_source="wastewater"
                     /db_xref="taxon:1463165"
                     /plasmid="pWP5-S18-ESBL-05_14"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6538 N 139.5126 E"
                     /collection_date="2018-08-06"
     gene            complement(101..337)
                     /locus_tag="H7R80_RS29795"
                     /old_locus_tag="WP5S18E05_P140010"
     CDS             complement(101..337)
                     /locus_tag="H7R80_RS29795"
                     /old_locus_tag="WP5S18E05_P140010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016230891.1"
                     /translation="MTRLTNLTPAEKKFIDDAIAAAERAAGKKLNQPNRHIVLNRARAQ
                     IESQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
     gene            1066..1212
                     /locus_tag="H7R80_RS30220"
     CDS             1066..1212
                     /locus_tag="H7R80_RS30220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_087777340.1"
                     /translation="MHEPPVQSDCCAFSGNYLLESNPERHDKTPLAAAIGKRVYKRLLM
                     QSS"
                     /name="replication initiation protein"
     gene            complement(1416..1976)
                     /locus_tag="H7R80_RS29800"
                     /old_locus_tag="WP5S18E05_P140020"
     CDS             complement(1416..1976)
                     /locus_tag="H7R80_RS29800"
                     /old_locus_tag="WP5S18E05_P140020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_140415667.1"
                     /translation="MIKINHEFSTFLDIARYEKIVRNNDLISTVLNLALLMENFTNIYI
                     KEISPEEFHDIFLDKKQKQHFKLNVAKSLGLPRSLADAITKLSGIRNNFAHKLDYELKD
                     EEIIEFEQYVEKITAKEICRSGALNYNEVDSFLSSGVKIKGSDDYTSNKVIGRLVNTTY
                     MLANKAAFFSIDEFNRRGILSLG"
                     /name="hypothetical protein"
     Typing          complement(496..676)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.352"
                     /coverage="83.0"
                     /evalue="4.29e-85"
     Typing          888..1344
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.895"
                     /coverage="89.0"
                     /evalue="1.05e-134"
ORIGIN
        1 aacgcccctg atgatttctg cgcgactgca tggctatgca cgcgggtgaa tcgcaggtga
       61 tttcgcagcg tcagcaccac taaaccgcca aaatccgcaa tcagcggcgc ggcgcgcgcg
      121 gacgtgacca ggcaaaatcg gactgctgcc gctcatcctc acgtaatgca cgctggcggt
      181 cggcgtaacg ctgcgactca atctgcgcac gcgctctgtt caggacgatg tgccggttag
      241 gctgattgag cttcttcccg gccgcacgtt cggcagcggc tatagcgtcg tcgataaact
      301 ttttctcagc gggggtcagg tttgtcagtc tcgtcatgtt tatctccgta tgagaatagt
      361 ccctgcagtc tgccagacac agtaggaacg atgaagcgta agcgacggtg aaaggcagtc
      421 agaaacggtg aacgggagcg gattttaatc cggtgccgtg aggcgttgga caagccgcag
      481 gcgcgtcagt ggatttagcg ggtttcgggg cgcagccctg aaccagtcac gtagcgctag
      541 cggagtgtat actggctaaa tatgttacgg cggagtggta atttatgaaa gtgcttcatg
      601 tgacaaggga gaaaatgcgg ctaccgggcg taagtgcaac atgcagttaa ggatgaaatg
      661 acgcctcctc gctcactcgg tcgctacgct cctgccgtga gactgcggcg ggcgttaccg
      721 gctcacagat gacgggacat gcaagcagta ctcaaatcag gaagggccgg agaaaggatg
      781 cggcgttgcc gttttttcat aggctccgcc cccctgccgg aattcataaa cagacacttt
      841 ttcggtgcgt ctgtgggagc ctgagctcaa ccatgaatcc gacagcggtg acgaaacccg
      901 acaggactta aagatcccca ccgtttccga tgggtcgctc cctcgtgcgc tctcctgttc
      961 ctgcctttcg gtttaccggt gtcattccgc tgttatggcc gcgtttgtct cattccacgc
     1021 ctgacactca gttccgggta ggcagttcgc tccaagctgg actgtatgca cgaacccccc
     1081 gttcagtccg actgctgcgc cttttccggt aactatcttc ttgagtccaa cccggaaaga
     1141 cacgacaaaa cgccactggc agcagccatt ggtaagaggg tgtacaagag attactgatg
     1201 cagagttctt gaagtctgag ccaaactaca gctaaactgg aaggacgctt ttggtggcag
     1261 cggtcttgta caccctgtta ccacggttca gaaacttgca ggtctctgat ccttcaaaaa
     1321 aaccgcctgg cagggcggtt ttttttgttc ttggaataag aagataccgc cacagataga
     1381 caatcacaag cagataaaat caaacctttg aaaaatcatc cgaggctcaa tattcctcta
     1441 cggttaaatt catctattga gaaaaaagca gctttgttag caagcatata tgtggtgtta
     1501 accaatctcc ctatcacttt gttagaggtg taatcatcgc tcccttttat cttcactcca
     1561 gatgataaaa acgaatcgac ttcattgtaa tttagagcgc ctgatctaca tatttctttc
     1621 gcggttatct tttctacata ctgctcaaac tcaataattt cttcatcttt aagctcataa
     1681 tctaatttat gcgcaaaatt attcctaatc cctgataact tagtgattgc atcggccaag
     1741 gatctaggca aaccaagcga tttagctacg ttgagtttga aatgttgttt ttgctttttg
     1801 tctaaaaaaa tatcgtgaaa ctcttctgga gaaatctcct ttatataaat attagtaaaa
     1861 ttctccataa gaagagctaa attcaagaca gtagaaatta aatcattatt tctaacaatc
     1921 ttttcatatc tagcaatatc taagaaagtt gaaaattcgt ggttaatttt aatcaaaata
     1981 ccccctctca gcttcataaa tttaaaatat tacttagtgc aaattactaa tttaaaaaaa
     2041 ttagcaactg aaaatcttat acgccgtata agactctctt ttgcacgtta ggtaaatcgc
     2101 atttaccata acatccgttg gccgcacccc ctgacaggcc ctcagcgcaa ttctggcgca
     2161 tcagagccgt ttccggcaca tttcctcacc cgcatcccgg aaa
//
