LOCUS       NZ_AP022155             2694 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella quasipneumoniae strain WP5-S18-ESBL-05 plasmid
            pWP5-S18-ESBL-05_13, complete sequence.
ACCESSION   NZ_AP022155
VERSION     NZ_AP022155.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194531
            Sequence Read Archive: DRR199367
            Assembly: GCF_014169355.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Klebsiella quasipneumoniae str.
            WP5-S18-ESBL-05 isolated from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 195x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 10:17:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,866
            Genes (coding)                    :: 5,626
            CDSs (with protein)               :: 5,626
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 79 of 240
            Pseudo Genes (incomplete)         :: 174 of 240
            Pseudo Genes (internal stop)      :: 45 of 240
            Pseudo Genes (multiple problems)  :: 48 of 240
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022155.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WP5-S18-ESBL-05"
                     /isolation_source="wastewater"
                     /db_xref="taxon:1463165"
                     /plasmid="pWP5-S18-ESBL-05_13"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6538 N 139.5126 E"
                     /collection_date="2018-08-06"
     gene            complement(101..706)
                     /locus_tag="H7R80_RS29780"
                     /old_locus_tag="WP5S18E05_P130010"
     CDS             complement(101..706)
                     /locus_tag="H7R80_RS29780"
                     /old_locus_tag="WP5S18E05_P130010"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_047359292.1"
                     /translation="MSFIKSLNKKNQTDQIEVTINVRLTQEEDHLLTLATQQSGYKRNE
                     LFYLLAKEYIFDDESLKEMAEEPGAEFSEGLYQNCYVVNTNRVHHEEDDKFMISEGVAA
                     TFEDGYKEKMEQVKKGDLVFLYRSGEGIVAHGIASGDVLKKDHNGVPDKTYYQKLSGFV
                     RYEKPLTASEIQRILGRNVVFMHTLFSIRDGYKLLKKD"
                     /name="hypothetical protein"
     gene            957..1925
                     /gene="mobV"
                     /locus_tag="H7R80_RS29785"
                     /old_locus_tag="WP5S18E05_P130020"
     CDS             957..1925
                     /gene="mobV"
                     /locus_tag="H7R80_RS29785"
                     /old_locus_tag="WP5S18E05_P130020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_119882091.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNADAERTPENYCS
                     VSQSADEAMGKLRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFAHSEQWLE
                     KKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMRDDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYQSINRGTRSQVSISPDALEPRVLRKGIFTKDVEDQAA
                     IAKRLSHAVNDGLAGTIAMASQSAQNAKRVRELQKTMDSQQKRLQSVTEPFKGLSREQI
                     TEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMSR"
                     /name="mobV"
     gene            complement(1948..2379)
                     /locus_tag="H7R80_RS29790"
                     /old_locus_tag="WP5S18E05_P130030"
     CDS             complement(1948..2379)
                     /locus_tag="H7R80_RS29790"
                     /old_locus_tag="WP5S18E05_P130030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_080490715.1"
                     /translation="MRRITQVDQTTGEELGGFVAVIRPKQKSSFQRHFTMNQAALITIA
                     NELNHDQMRVLMALLAELDYENYIQVAQMDIAEALTMQKTNVSRAVKNLIDFGIILEGP
                     KIGRSKTYRLNPQFGWKGTVSNHKKALKNGLSVIQGGRS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          910..1922
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.704"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agcgtagcga gttcatttac ccttgacgat gcactgtaaa aaacacttcc ggaagtgtgg
       61 ttggtgtcac aaagtgatgc caaccacctg tagactgggt ttaatctttt ttaagcagtt
      121 tatagccatc tctaatgcta aatagcgtgt gcataaacac aacgtttctc cccagtattc
      181 tctggatctc agacgcggtt agtggttttt cgtacctaac aaagcctgat agcttctggt
      241 agtaggtctt gtctggcacg ccattatgat ctttctttaa aacatcacca ctggcgatac
      301 catgtgcaac aattccttcg ccggagcgat aaagaaagac taaatctcct tttttaacct
      361 gctccatttt ttccttataa ccatcttcaa atgttgccgc tacgccctct gaaatcatga
      421 acttatcatc ttcttcatga tgaactctgt tagtgttaac cacataacaa ttttggtata
      481 agccttctga aaactcagcg ccaggctcct cagccatttc ctttaagctt tcatcatcaa
      541 agatgtattc ctttgctagg aggtagaata gctcgttcct tttataacca gattgctggg
      601 ttgctaatgt cagtaaatgg tcctcttctt gggttaacct aacgttaatc gtcacttcta
      661 tttggtctgt ttgatttttt ttgtttagcg attttatgaa tgacatctta ctttcctcac
      721 cagagttaat gtactaatca ataatagtga atcactatat tgaaacagaa gcattgatca
      781 catctttgat ttaagcaatg actttatgtc atatattttt tattaagttg aatttaacga
      841 acgattttgc gtagcaaagt ctagtgagta cactatcatc ctgatagtgg cccgccggag
      901 gcatcgcaat gcgatccccc gactggctgg atgtacgcca gaacgagggg gtaagcatgg
      961 cgaattacgc gataatgcgc tgcaaaaagc tgacgggaat gggcagtgtg gccagcgctt
     1021 tgcagcactg ctacagggag cgtgagacgc cgaatgctga cgctgaacgg acaccagaaa
     1081 actactgctc tgtgtctcag tcggcagatg aggcgatggg aaagctgcgg gagctgttgc
     1141 cggaaaaacg ccgtaaagat gcggtactgg ctgttgagta cgttatgacg gcaagccctg
     1201 agtggtggaa agaagcgaca ccgcggcagc aggcggagtt tttcgcccat tctgagcagt
     1261 ggctggagaa gaaatacggg aaggatcgtg ttgtggctgc ggtagttcac cgggacgagg
     1321 ccaccccgca tttgtcagcg ttcgtggtgc cattgacgca ggatggacgg ctgtccgcga
     1381 aagagtttat tggcgggcgg tcaaagatgc gtgacgatca gagcacctac gctgaatcag
     1441 taaaaaaatt gggtcttgag cgcggcatcg agggaagccg tgccacgcat cagacagttc
     1501 agcactacta ccaatcgata aaccgtggca cccgtagcca ggtatcaatc tcgccagatg
     1561 cgctggagcc gcgcgtattg cggaaaggaa ttttcaccaa agacgtggaa gatcaggcag
     1621 ccatagccaa acgtctaagt cacgcagtga acgatggcct tgcaggaacc atcgccatgg
     1681 cctctcagag cgcgcagaat gcaaaaagag tgcgcgagct acagaagact atggactcgc
     1741 agcagaaacg cctacagagc gttacagagc cttttaaggg cctttccaga gagcagataa
     1801 ccgaaatcct gatgatggct cagcggttta agcagcaaaa ccaggaaaag gaaaaacagc
     1861 agcgtattga acgtgaaaaa cagcgacaga cgcgatcgcg tggcatgggt ggtatgagtc
     1921 gttagttttg atgcgacatt ccagagatca gcttctaccg ccctgtataa cgctgaggcc
     1981 gttttttagt gcttttttgt ggttgctgac agtacctttc caaccgaact ggggattcaa
     2041 gcgataagtt ttgctccgtc ctatcttagg accttccagt atgatcccga aatcaatcag
     2101 gttttttacc gcacgactga catttgtttt ttgcatagtc agcgcttccg caatatccat
     2161 ctgcgcaact tggatgtagt tctcgtaatc cagttctgcg agcaaggcca tcagcacacg
     2221 catctggtca tggtttagct cattggcaat ggtaattagc gcggcttggt tcatagtaaa
     2281 gtgcctctgg aatgatgact tttgtttagg acgaatcact gctacaaaac cacctaattc
     2341 ctcccctgta gtttggtcta cctgcgtgat tctccgcatc taaaaatctc cattcaggaa
     2401 aaaatagtta tctctcaaga taactaagtt atctctagag ataactattg tcaaaaactt
     2461 tccgcttgtg gcgcggcttc ccagccatcc ccttctgtat cttattactc tccacaatca
     2521 cacatcggca aaatcaggtg cttttatgcc tgttctgagt gttagggttc ctatgtaagt
     2581 gacgtggaaa ttccgcttcc tcgctcattc gctcactacg ttcggtcatg agtctgcggc
     2641 gagcggttct tgatttttta aaacgatatt cggagcttta tttcgtcaag ggcg
//
