LOCUS       NZ_AP022154             2726 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella quasipneumoniae strain WP5-S18-ESBL-05 plasmid
            pWP5-S18-ESBL-05_12, complete sequence.
ACCESSION   NZ_AP022154
VERSION     NZ_AP022154.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194531
            Sequence Read Archive: DRR199367
            Assembly: GCF_014169355.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Klebsiella quasipneumoniae str.
            WP5-S18-ESBL-05 isolated from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 195x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 10:17:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,866
            Genes (coding)                    :: 5,626
            CDSs (with protein)               :: 5,626
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 79 of 240
            Pseudo Genes (incomplete)         :: 174 of 240
            Pseudo Genes (internal stop)      :: 45 of 240
            Pseudo Genes (multiple problems)  :: 48 of 240
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022154.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WP5-S18-ESBL-05"
                     /isolation_source="wastewater"
                     /db_xref="taxon:1463165"
                     /plasmid="pWP5-S18-ESBL-05_12"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6538 N 139.5126 E"
                     /collection_date="2018-08-06"
     gene            complement(320..871)
                     /locus_tag="H7R80_RS29765"
                     /old_locus_tag="WP5S18E05_P120010"
     CDS             complement(320..871)
                     /locus_tag="H7R80_RS29765"
                     /old_locus_tag="WP5S18E05_P120010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_024262791.1"
                     /translation="MSLVRYKTNPFLDDMIVPVKGQKVQISKLGKDNNILVNQSTGEVH
                     GTHVTTYRKVDSEQFVKLFTANIALTFQLSSAGIKAFGVLLWVVQNKALSKDEVDLDSY
                     ALDEFYELHKDSEPPLRLSHPTFKRGINELEKAQIIAKTLRKGRYFINPNFVFNGDRVA
                     FTTVIERKKAETPEIKEISS"
                     /name="replication/maintenance protein RepL"
     gene            1478..2446
                     /gene="mobV"
                     /locus_tag="H7R80_RS29770"
                     /old_locus_tag="WP5S18E05_P120020"
     CDS             1478..2446
                     /gene="mobV"
                     /locus_tag="H7R80_RS29770"
                     /old_locus_tag="WP5S18E05_P120020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852935.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_176217771.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNADAERTPENYCS
                     VSQSADEAMGKLRELLPEKRRKDAVLAVEYVMTASPEWWNEATPRQQAEFFARSEQWLE
                     KKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSQAVNDGFAGTIAMASQSAQNAKRARDLQKTMDSQQKRLQSVTEPFKGLSREQM
                     THILTMAQTFQQQNHDREKQRQLEREQERQQRQKTDRGISR"
                     /name="mobV"
     gene            2443..2634
                     /locus_tag="H7R80_RS29775"
                     /old_locus_tag="WP5S18E05_P120030"
     CDS             2443..2634
                     /locus_tag="H7R80_RS29775"
                     /old_locus_tag="WP5S18E05_P120030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_023063538.1"
                     /translation="MSEKQRREGFRKAEASLRLEGLDPSGTPHYELVKARIISGEISYE
                     QGRKEIFDYYVQKARSKE"
                     /name="antitoxin VbhA family protein"
     Typing          1431..2341
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.053"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 ttataatata taaagtgtct gttacttttt atttatattt ttaagtaaga agaacggcga
       61 gtaattccgt caatataaat aataaaatat gaggctacgc atgaataaaa aagaaaaagt
      121 agccaagcgt atgagaataa taataaaaat aacgattgtt attatttcta tgtatccttg
      181 gtctgtttgt tggtgaaaat aaaaaagcag cttttagctg cttttttatt ttggatgcat
      241 ccgttttcca ttagttacac agggtaggca tgctgcggat gcttgatagg tagtccttgg
      301 ttgagttctg tgagtcttat caagacgaaa tctcttttat ttctggggtc tctgcttttt
      361 ttctttcaat gacagttgtg aaagcaactc tatcgccatt aaatacaaag ttggggttaa
      421 taaaatagcg acctttccga agtgtttttg caataatctg ggctttttct aattcgttta
      481 tcccccgttt aaaagtaggg tggcttagtc gcagtggggg ttctgagtcc ttatgtaatt
      541 cataaaactc atctaaggca tatgaatcta aatcgacctc atcttttgag agagctttgt
      601 tttgaacaac ccataataaa accccgaatg cttttatacc tgctgatgac aactggaaag
      661 ttaaagcaat attggctgtg aaaagcttta cgaactgctc tgaatcaacc tttcgatatg
      721 tagttacgtg cgttccgtgt acctcacctg tcgattggtt aaccaagata ttgttgtctt
      781 ttcccagctt agagatctgc actttctgtc cctttactgg aacaatcata tcatccaaaa
      841 atgggttagt tttgtatctt actaagctca ttatccaccc ataaattgat accatcaatt
      901 cgattttaga tagtcacaag ctcaaaatca acaactattt tgagcttaca aagtacgtag
      961 attgagcctt gaaggtactg attttgaaaa tacgatcctt gctacatgcg ggtttgcgag
     1021 tagtcccttc ttagttctta tctatcgcta agaactaaga agaaaaaaca gaggtcctga
     1081 gcgaaagtgc ctgaggtagt ttccgtgata ttccgcttcc tcgaacggac agactcgcgg
     1141 cgatgccgct ccctaatccg ttcttgcggc gagcggttac tgctctttca aaaacgatat
     1201 tcattgcctg atttcgtcag gggcgagcga agcgagtgcc atttaccctt gacgactcac
     1261 cggataaaac aggcttggca gtggtggggg gtggcgcgga gcgacgcctt ccacctgcca
     1321 gaccgccgtt gattttgacc ttctggcagc aagctgcccg aacgattttg cgtagcaaag
     1381 tctagtgagt acactatcat cctgatagtg gcccgccgga ggcatcgcaa tgcgatcccc
     1441 cgactggctg gatggacgcc agaacgaggg ggtgagcatg gcgaattacg cgataatgcg
     1501 ctgcaaaaag ctgacgggaa tgggcagtgt ggccagcgct ttgcagcact gctacaggga
     1561 gcgtgagacg ccgaatgctg acgctgaacg gacaccagaa aactactgct ctgtgtctca
     1621 gtcggcagat gaggcgatgg gaaagctgcg ggagctgttg ccggaaaaac gccgtaaaga
     1681 tgcggtactg gctgttgaat acgttatgac ggcaagccct gagtggtgga acgaggccac
     1741 accgcggcag caggcggagt ttttcgcccg ctctgagcaa tggctggaga agaaatacgg
     1801 gaaggatcgc gttgtggcgg cggtggttca ccgggacgag gccaccccgc acttgtcagc
     1861 gttcgtggtg ccattgacgc aggatggacg gctgtccgcg aaagagttta ttggcgggcg
     1921 gtcaaagatg cgtgaagatc agagcaccta cgctgaatca gtaaaaaaac tgggtctcga
     1981 gcgcggcatc gagggaagcc gcgccacgca tcagacagtt cagcactact acgaatcgat
     2041 aaaccgtggc acccgtagcc aggtatcgat ctcgccggag gcgctggagc cgcgtgtact
     2101 gcggaagggt attttcacca aagacgtgga agatcaggca gccatagcca aacgtttaag
     2161 ccaggcagtg aacgatggct ttgcaggaac catcgctatg gcctctcaga gcgcccagaa
     2221 tgccaaaaga gcgcgcgatc tacagaagac tatggattcg cagcaaaaac gcctacagag
     2281 cgttacagag ccttttaagg gactttcccg ggagcagatg acccatattc tgacgatggc
     2341 gcagacgttc cagcaacaga atcacgatcg tgaaaaacag cgccagcttg aacgggagca
     2401 ggaacgccag caacgtcaga aaactgacag aggtattagc cgatgagcga aaaacagcga
     2461 cgtgaaggtt ttcgcaaagc ggaagcctca ttaaggctcg aagggctgga tcccagcgga
     2521 acgcctcact atgagttagt taaagcgcgc attatttcgg gtgaaatcag ctatgaacag
     2581 ggacgaaagg agatttttga ttattacgtt cagaaagctc gctccaaaga ataagaactc
     2641 atattaagac acaatattta tatttattta aaagcgtgaa aatatatttt ccattttata
     2701 taaaatatat ttgcttttta tgttta
//
