LOCUS       NZ_AP022153             3071 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella quasipneumoniae strain WP5-S18-ESBL-05 plasmid
            pWP5-S18-ESBL-05_11, complete sequence.
ACCESSION   NZ_AP022153
VERSION     NZ_AP022153.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194531
            Sequence Read Archive: DRR199367
            Assembly: GCF_014169355.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Klebsiella quasipneumoniae str.
            WP5-S18-ESBL-05 isolated from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 195x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 10:17:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,866
            Genes (coding)                    :: 5,626
            CDSs (with protein)               :: 5,626
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 79 of 240
            Pseudo Genes (incomplete)         :: 174 of 240
            Pseudo Genes (internal stop)      :: 45 of 240
            Pseudo Genes (multiple problems)  :: 48 of 240
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022153.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WP5-S18-ESBL-05"
                     /isolation_source="wastewater"
                     /db_xref="taxon:1463165"
                     /plasmid="pWP5-S18-ESBL-05_11"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6538 N 139.5126 E"
                     /collection_date="2018-08-06"
     gene            <2041..2196
                     /locus_tag="H7R80_RS29755"
                     /pseudo=""
     CDS             <2041..2196
                     /locus_tag="H7R80_RS29755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707212.3"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            2290..2934
                     /locus_tag="H7R80_RS29760"
                     /old_locus_tag="WP5S18E05_P110020"
     CDS             2290..2934
                     /locus_tag="H7R80_RS29760"
                     /old_locus_tag="WP5S18E05_P110020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrB19"
                     /protein_id="WP_012954666.1"
                     /translation="MTLALVGEKIDRNRFTGEKVENSTFFNCDFSGADLSGTEFIGCQF
                     YDRESQKGCNFSRAMLKDAIFKSCDLSMADFRNVSALGIEIRHCRAQGADFRGASFMNM
                     ITTRTWFCSAYITNTNLSYANFSKVVLEKCELWENRWMGTQVLGATFSGSDLSGGEFST
                     FDWRAANFTHCDLTNSELGDLDIRGVDLQGVKLDSYQASLLMERLGIAVIG"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrB19"
     ARG             2291..2931
                     /name="qnrB19"
                     /gene="qnrB19"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrB19"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(1139..1312)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          1291..1782
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.51"
                     /coverage="81.0"
                     /evalue="0.0"
ORIGIN
        1 ttctggacag gaatttgatg aatatctaac caattcattt tcgcgagaac aacgaataac
       61 cactcccgaa tcatgaagcc atgtcttaac gaaaatatgg ctaactcata gcaaaaaaaa
      121 agccccctaa gggctttttt ttgtacctga cggtcagacc gattaaacat aacatccctt
      181 gcgtgcaccg cctgagaggc tctgagggcg tttctgacgg gaaaccggta aaacaggcaa
      241 caaaccaccc gaaaacccgc cagaaacgcg caggctctgt ttttacggca tgcatgagta
      301 tgcgcgcaag tgcatagcgc ggaaattcac accattcagc caggggaagt cctgtttccg
      361 gcgttcttcc catccgaaaa cggtgaacgg ggacgcaatt tattgcgtgg ccgtgaagcg
      421 ttgccggcgc agccggaaaa cccgctgtca gcacgttcag gaagggggtg atgtacaaat
      481 ggcacagagg atgaaagtga cgtatgagct gaaggaatga tgtaaaagtg atgtataaat
      541 gatgtaaaag tgatgcaaaa gtgttgtaaa agtgatgtac aggccgaagg agtgttgtaa
      601 aagtgttgta aaagtgatgt atgaactgaa ggagcgatgt aattctgtag tcaggaagaa
      661 taacccgttc atgcaggcat gaacgataca gtgaggaacg cttttttttg tcacgatctc
      721 cccttaacct gcgtaaatca cccgccatgg ttgattattt ttcaacctca aaatggtcat
      781 acacctaacg cagccgcgat tttcatcgca actgcatgaa ttaaaagaaa aaaaagtaat
      841 gtttcgtttt cacgagtaat acccaggtat taaaaaccca ctgtaattac atgaaaataa
      901 agaaataaaa ggtaataccg gcgtattacc acgtacgcgg gcaagcgaaa aagctataac
      961 gcttcccggc cgacaggcgg cccagaatcc acaggggcat gcaggatctc ttcagtaacc
     1021 ttcgccatta aatcaaaaag gtcatattcg ccgggaagct gcgtatcagt cgaaccccgt
     1081 tgttcatcat attcagatgg catggggtga tatccagtag cagagaggtt gttagcgggt
     1141 ttcggggcgc agccctgaac cagtcacgta gcgctagcgg agtgtatact ggctgactat
     1201 gttggcacgg atgtggaaat cagtgaagtg cttcatgtag caggagaaaa aaggcggcac
     1261 cagcgcgtca gcaggatatg tagtacagga tatattccgc ttcctcgctc actgactcgc
     1321 tgcgctcggt cgttcggctg cggcgagcgg tgccggctta caggcggggc ggaaatttcc
     1381 tgggagatac caggaaggag cttaacaggg aagagataag gccgcggcga agccgttttt
     1441 ccataggctc cgcccccctg acaaacatca tgaaatctga cgctcaaatc agtggtggcg
     1501 aaacccgatg cggcttaaag ataccaggcg tttccccctg gtggctccct cgtgctcccc
     1561 cgcattcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg tttatctcat
     1621 tccacgcctg acactcagtt ccgcgaaggc agttcgctcc tggatggact gtatgcacga
     1681 accccccgtt cagtccgact gctgcgcctt atccggtaac tatcatcttg agtccaaccc
     1741 ggaaagacac gacttatcgc cactggcagc agccactggt aactgattta gaggagttag
     1801 tcttgaagtt atgcgcctgt taaggctaaa ctgaaagaac agatttggtt actgcgctcc
     1861 tcgtaagcca gttaccacgg ttcaaagagt tgctgctctg agaaccttcg aaaaaccgcc
     1921 ttgcagggcg gttttttcgt ttacagagca agagattacg acgccacaga aaggatctca
     1981 agaagatcct tcaccgtaat ggaaatctat ccaatccgag cacataggat acacatcgtt
     2041 ataacgctgg atctgcgtga attccagcag cagcaagaga aagactttct acaaaccagc
     2101 ttacagcagg ctaaatttaa ccagaaaaaa gccgctgaat tactgggtct gacttatcat
     2161 cagctacgtg ccttgctgaa gaaacaccag atctaatacg catttgcagg tgtcatactg
     2221 gcgggtttga cgcataacgt cataaggttt accatggcgt cattactgta taaaaacaca
     2281 ggcatagata tgactctggc attagttggc gaaaaaattg acagaaatcg cttcaccggt
     2341 gagaaagttg aaaatagtac attttttaac tgcgattttt caggtgccga cctgagcggc
     2401 actgaattta tcggctgcca gttctatgat cgcgaaagtc agaaagggtg caattttagt
     2461 cgcgcaatgc tgaaagatgc cattttcaaa agctgtgatt tatcaatggc agatttccgc
     2521 aacgtcagtg ccttgggcat tgaaattcgc cactgccgcg cacaaggcgc agatttccgc
     2581 ggtgcaagct ttatgaatat gatcaccacg cgcacctggt tttgcagcgc atatatcact
     2641 aatactaatc taagctacgc caatttttcg aaagtcgtgt tggaaaagtg tgagctgtgg
     2701 gaaaaccgct ggatggggac tcaggtactg ggtgcgacgt tcagtggttc agatctctcc
     2761 ggcggcgagt tttcgacttt cgactggcga gcagcaaact tcacacattg cgatctgacc
     2821 aattcggagt taggtgactt agatattcgg ggtgttgatt tacaaggcgt taagttagac
     2881 agctaccagg catcgttgct catggagcgg cttggcatcg ctgtgattgg ttagttcagc
     2941 gtttacaccg ggagatgcgt ttgacgattt acttttaacc agtacgactc ctggcaagca
     3001 ttgtgaaacg ttttctccag tggacgaata tcctgaccta ccgcatcaac gacatgccat
     3061 ctaatgctgc a
//
