LOCUS       NZ_AP022152             4048 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella quasipneumoniae strain WP5-S18-ESBL-05 plasmid
            pWP5-S18-ESBL-05_10, complete sequence.
ACCESSION   NZ_AP022152
VERSION     NZ_AP022152.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194531
            Sequence Read Archive: DRR199367
            Assembly: GCF_014169355.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Klebsiella quasipneumoniae str.
            WP5-S18-ESBL-05 isolated from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 195x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 10:17:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,866
            Genes (coding)                    :: 5,626
            CDSs (with protein)               :: 5,626
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 79 of 240
            Pseudo Genes (incomplete)         :: 174 of 240
            Pseudo Genes (internal stop)      :: 45 of 240
            Pseudo Genes (multiple problems)  :: 48 of 240
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022152.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WP5-S18-ESBL-05"
                     /isolation_source="wastewater"
                     /db_xref="taxon:1463165"
                     /plasmid="pWP5-S18-ESBL-05_10"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6538 N 139.5126 E"
                     /collection_date="2018-08-06"
     gene            101..448
                     /gene="mobC"
                     /locus_tag="H7R80_RS29740"
                     /old_locus_tag="WP5S18E05_P100010"
     CDS             101..448
                     /gene="mobC"
                     /locus_tag="H7R80_RS29740"
                     /old_locus_tag="WP5S18E05_P100010"
                     /inference="COORDINATES: protein motif:HMM:NF017524.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_048295903.1"
                     /translation="MSYKDRYNRKHSITLCFNDEEFAQVEELASMLKKPRATAVRDLIL
                     NKDFKLKKEIAKNKTGDVYFELHKIGVNINQIARAINTDIDKFISHEFSEDFLNLFEEI
                     TNEIEEIKRRI"
                     /name="mobC"
     gene            450..1868
                     /locus_tag="H7R80_RS29745"
                     /old_locus_tag="WP5S18E05_P100020"
     CDS             450..1868
                     /locus_tag="H7R80_RS29745"
                     /old_locus_tag="WP5S18E05_P100020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_127795828.1"
                     /translation="MYHKIIGGKKSKGQRNIKKSIEYLLREKNPEQQKDIKILSTTTKQ
                     DLINFENYILDKNKSNPYVCGVLSFEEKSISEELKTKLITEFEDILFAGIEQENRPPVM
                     WVEHNDKGRVELNYLTFNSLQDRRSYTVYLDKRDRGLVNNYSEIINYENNLSSPFEEKE
                     NKNTLTNKPKTNIPEDKKQFIENINTEIMALIINEELENREQTIKYIENMKGVKINRIR
                     KNQISIKSDLFDDDKPIVLKGDIYEEGRDYSDYRKEFNPKPTRDPEFVREKLTELKRTF
                     AERIEKREIRNRSKFKKPSLKNIPENRQSVQREDQDIGYKYTNGDFINVSNNDGFSGMY
                     IRTDNMEIVNNESINTEYKTKTATEETAGTTRTRDIEKINQQQQFVNSRLEEAGRKQQQ
                     FRKSIDTTEGRFGVCRRSVHSIISTFGRYIRTFSAYTRKNEHRQKFEKRRKIEEEKLKI
                     KNQGSYNRRRYKPY"
                     /name="hypothetical protein"
     gene            2484..3896
                     /locus_tag="H7R80_RS29750"
                     /old_locus_tag="WP5S18E05_P100030"
     CDS             2484..3896
                     /locus_tag="H7R80_RS29750"
                     /old_locus_tag="WP5S18E05_P100030"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_113519630.1"
                     /translation="MIRLKKVFNLTEEEIVLTNEIAEAISKEPTVETHEVNINFIDVLP
                     IIPNPHNLGLSKDIDPLSLIYSKFITQTDIPPALPVFSFLSYLSAFSVNNNIMYKHPTS
                     PADYLNTWTLILAPSGAAKTTAAKIISSSIPKDIEENPMIKPNFEGADGSAAFISELAK
                     AEKKIDNFGKPIQPIFWIEDEYSQFMKKLMPGGSMVETRKTMLKIHDNDKARRVTKNDT
                     IETESIVMSGLFLNTIDSFARNFDQESINDGLGRRHNFVYAERGEKVVPTWTVDQIIES
                     LKEGLDDFFTTVKTNVIYTYSPECRKIYDHFYMVYKEKFDHILGEETNGTFFRTYFMLS
                     WKYAAIYHILLKQEGTEIQAKSFDYGIKVSLMFLSSIKRFLDYKVDLKSGAKQVRKEYL
                     QKAAEKELNNLQKYKEFLLDNIEISLRDFNRKFNIKKIESLKILKTIRENKLIKYHPLF
                     EVLAKEEAKAKK"
                     /name="hypothetical protein"
ORIGIN
        1 tggaaaacta tataacagca agctcagaca atacttcgta ttgtcgcttg tgaggggttc
       61 ccccttcaat ccccttaaaa cccaacacag gaggcatagg atgagctaca aggatagata
      121 caacaggaag cacagcataa ccctgtgctt caatgatgaa gaatttgcac aggttgaaga
      181 actggcttct atgttgaaaa aacccagagc aacagccgtt agagatctta ttctcaataa
      241 agattttaaa ctcaaaaaag aaatagcaaa aaataaaact ggtgatgtat attttgaact
      301 ccataaaata ggagtaaaca ttaaccagat tgcaagagca ataaatactg atattgataa
      361 atttatttcc catgagtttt cagaagattt tttaaatttg tttgaggaaa taacaaatga
      421 gatagaagaa ataaaaagga gaatataata tgtaccataa aataatagga ggtaaaaaaa
      481 gcaaaggtca aaggaatata aaaaaaagta ttgaatattt attgcgagag aaaaacccag
      541 aacaacagaa agacataaaa atattatcaa caaccacaaa acaagaccta atcaattttg
      601 aaaattatat tttagataaa aacaaatcaa acccatatgt gtgtggtgtt ttatcatttg
      661 aagaaaaatc catatccgaa gaattaaaaa ctaaattaat tacagagttt gaagatattt
      721 tatttgcagg gattgaacaa gaaaacagac ctccagttat gtgggtagaa cacaacgaca
      781 agggcagggt agagttaaat tatttaacat ttaacagttt acaagacaga agatcatata
      841 ctgtatattt agataaaaga gatcgtggat tggtaaataa ctactctgaa ataattaatt
      901 atgaaaataa tttatcaagt ccattcgaag agaaagaaaa taaaaacaca ttaaccaaca
      961 aaccaaaaac caatattcca gaagataaaa aacaatttat tgaaaatatt aatactgaaa
     1021 taatggcatt aattattaat gaagagttgg aaaatagaga acaaaccatc aaatatattg
     1081 aaaatatgaa aggtgttaaa ataaacagaa tacgaaaaaa tcaaatatca attaaatctg
     1141 atttgtttga tgacgataaa cccattgtcc tgaaaggaga tatatatgaa gagggaagag
     1201 attacagcga ttatagaaaa gaattcaacc caaaacctac aagagatcca gagtttgtta
     1261 gagaaaaact cacagagctt aaaagaacat ttgcagaaag aattgaaaaa cgagaaatca
     1321 gaaatagatc aaagtttaaa aaaccatcgc tcaaaaatat accagaaaat agacaaagtg
     1381 ttcaaagaga agatcaagat attggatata aatatactaa cggcgatttt atcaatgtta
     1441 gtaataacga tggtttttca ggtatgtata ttcgcactga taatatggag attgtaaaca
     1501 atgagtcaat taatacagaa tataaaacta aaacagcaac agaagaaaca gcaggaacta
     1561 caagaacaag agatattgaa aaaattaatc agcaacaaca atttgttaat tcgagacttg
     1621 aagaagctgg aagaaaacaa cagcaattca gaaagtcgat tgatacaact gagggaagat
     1681 ttggagtatg tagaagatct gtacactcaa ttatttctac ttttggaaga tacatacgaa
     1741 cgttttcagc atatacaagg aagaatgaac acagacaaaa attcgaaaaa agacgaaaaa
     1801 tcgaagaaga aaaactcaaa atcaaaaatc agggaagtta caacagaaga cgatataaac
     1861 catattaaaa atatgatgac aacactacta gatcactatg ataaaacttt aaccgtgaac
     1921 aagtgagagc cagccgtcag gctggttctt gcttgtgagg ggttgacctg tccggtagga
     1981 cggttttctg tcagaaatct gtcagagtgt cagaaaaaac caagaaaact tgtcagatat
     2041 tttccaaaaa agatccgaca ccttaaaatc cattgagtca aaaataaaaa cgggatattt
     2101 tatgacaaaa caggttataa aattgcctgt ttttttaatt tttgattttg tcataaaact
     2161 aatgacaccg agaaagatta ttttttcttt atatatcaat atattacgaa aaatgtcttc
     2221 tcttttgtca aaggttgtta gattttttat gacaaatcta tgacaaaaat ttttttaaac
     2281 cctttaaaat cagtatgtta cgtgatttgt cataatgtca tagtgtcaaa cagggggggg
     2341 gtggtatgtt tttggcttcg caaaaaccaa ccaaaaaata agacattttg gaatgtgtca
     2401 tatttttttg taaaaatatc attgacaagt taaaacctta tgttataact aattatataa
     2461 tagatataat aggagatttt attatgataa gattaaaaaa agtttttaat ttaacagaag
     2521 aagaaatagt tttaacaaac gaaatagcag aagcaattag caaagagcca acagtagaaa
     2581 cccacgaagt gaatataaat tttattgatg ttttacctat aataccaaac ccccataatt
     2641 taggtttatc aaaagacata gatccattat ctttaatata ttctaaattt attactcaaa
     2701 cagatatacc gcctgcttta cctgtattct cttttttaag ttatttaagt gcttttagtg
     2761 ttaataataa tataatgtat aaacacccta caagcccagc ggattacttg aatacttgga
     2821 ctttaatact tgcaccgtca ggtgcagcaa aaaccacagc agccaaaatt atttcaagct
     2881 ctataccaaa agatatagaa gaaaacccaa tgattaaacc caattttgaa ggtgctgacg
     2941 gttcggcagc attcatttca gagttggcaa aagcagaaaa gaaaattgat aatttcggaa
     3001 aaccaataca gccgattttc tggatagaag acgaatactc ccagtttatg aaaaaattaa
     3061 tgccgggagg ttctatggtt gaaaccagaa aaacgatgtt aaaaatccat gataatgata
     3121 aagcccgtag agttacgaaa aacgatacta ttgaaactga aagtatcgtt atgagtggtt
     3181 tatttttgaa tactattgat agttttgcga gaaactttga tcaggaaagt attaatgatg
     3241 ggttaggaag aagacataat tttgtttatg ctgaaagagg ggaaaaagtt gtcccaactt
     3301 ggacagtaga tcagatcatc gaatctctca aagagggatt agatgatttc ttcactactg
     3361 ttaaaacaaa cgtaatctat acatattccc cagaatgtag aaagatttat gatcattttt
     3421 atatggttta taaggaaaaa tttgatcata tattaggaga agaaacaaac ggaacattct
     3481 tcagaactta ttttatgttg agttggaaat atgcagcaat atatcatatt ttattaaaac
     3541 aagaaggaac cgagattcag gcaaaatctt ttgattatgg gattaaagtt tcattaatgt
     3601 ttttatccag tataaaaagg tttttagatt ataaagtaga tttaaaatct ggtgctaaac
     3661 aagtaaggaa agaatattta caaaaagcag cagaaaaaga attaaataat ttacaaaaat
     3721 acaaagagtt tttgcttgat aatattgaaa tttcattaag agacttcaat agaaaattta
     3781 atattaaaaa gatcgaaagt ctaaaaatac tcaaaacaat cagagaaaat aaattaataa
     3841 aatatcatcc attgtttgaa gtgttagcaa aagaagaagc caaagcaaaa aaataaaacc
     3901 gtcctaccgg acaggtcaac ccctcacaaa aaagccattt tacaaatggc tttatttgtt
     3961 cacggttata tataaaaccc aaatttgatt tgattatttt ttattcaggt attgacttaa
     4021 tttttatatg tattaattaa tatataag
//
