LOCUS       NZ_AP022150             4160 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella quasipneumoniae strain WP5-S18-ESBL-05 plasmid
            pWP5-S18-ESBL-05_8, complete sequence.
ACCESSION   NZ_AP022150
VERSION     NZ_AP022150.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194531
            Sequence Read Archive: DRR199367
            Assembly: GCF_014169355.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Klebsiella quasipneumoniae str.
            WP5-S18-ESBL-05 isolated from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 195x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 10:17:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,866
            Genes (coding)                    :: 5,626
            CDSs (with protein)               :: 5,626
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 79 of 240
            Pseudo Genes (incomplete)         :: 174 of 240
            Pseudo Genes (internal stop)      :: 45 of 240
            Pseudo Genes (multiple problems)  :: 48 of 240
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022150.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WP5-S18-ESBL-05"
                     /isolation_source="wastewater"
                     /db_xref="taxon:1463165"
                     /plasmid="pWP5-S18-ESBL-05_8"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6538 N 139.5126 E"
                     /collection_date="2018-08-06"
     gene            122..442
                     /locus_tag="H7R80_RS29690"
                     /old_locus_tag="WP5S18E05_P80010"
     CDS             122..442
                     /locus_tag="H7R80_RS29690"
                     /old_locus_tag="WP5S18E05_P80010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_024243919.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARAGRLPSI
                     SPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAVLETGGDD
                     DR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            651..>1082
                     /locus_tag="H7R80_RS30120"
                     /pseudo=""
     CDS             651..>1082
                     /locus_tag="H7R80_RS30120"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1120..1605
                     /locus_tag="H7R80_RS29700"
                     /old_locus_tag="WP5S18E05_P80020"
     CDS             1120..1605
                     /locus_tag="H7R80_RS29700"
                     /old_locus_tag="WP5S18E05_P80020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_064181052.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEKSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYQTIQAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGSYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            1609..1821
                     /locus_tag="H7R80_RS29705"
                     /old_locus_tag="WP5S18E05_P80030"
     CDS             1609..1821
                     /locus_tag="H7R80_RS29705"
                     /old_locus_tag="WP5S18E05_P80030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2507..3424
                     /locus_tag="H7R80_RS29710"
                     /old_locus_tag="WP5S18E05_P80050"
     CDS             2507..3424
                     /locus_tag="H7R80_RS29710"
                     /old_locus_tag="WP5S18E05_P80050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_116955814.1"
                     /translation="MSNAALHLFNERLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRAGAAIDWSDRNAPAPTLTITNPDNGHAHLLYALKTSIRTAPDGKIK
                     PLRYAAAVENALRKKLEADAGYSGLICKNPNHAHWKIAVWQPELYTLDWLADFLELNAA
                     NDKEIIADYGLGRNCTLFDKTRKWAYRAIRQGWPEYEQWLKACYERASAYNLQFSAPLD
                     DKEVMGIAKSIAKWTGSNFSKEAFDEYVIRTHTSDIQRIRGRKGGAIGGKKSNSGGRPS
                     LGEPWIELGVSRRTYFRNKKRKGC"
                     /name="replication initiation protein"
     Typing          433..1937
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.825"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccgaaacccg ctaaaagcga ctgacgcgcc tgcggcttgt ccaacaccgt gccgcccggg
       61 aaaaatttcc cgcccgtccc ggtgttatca ggaggctact gtggcagata aacgcaacaa
      121 gatgctcacg atgtgggtga cggaagacga acaccggcgg ctgctcgagc ggtgcgacgg
      181 caggcagctg gcggcgtgga tgcgtcagac ctgcctcgat gagaagccgg cacgtgccgg
      241 cagactgcct tcgatatcgc ctgcgctgct gcgccagctt gccggtatgg gtaacaacct
      301 gaaccagatc gcccggaaag tgaatgccgg tggtgccggc catgatcggg tgcagatcgt
      361 ggccgccctg atggcaatcg acgcgggact ggagcggctg cggcatgcgg tgctggaaac
      421 agggggggat gatgatcgtt aaattccatc cccgggggcg tggtggtggt gccggtccgg
      481 tggattacct gctgggaaaa gaccggcagc gcgagggggc gaccgtgctg cagggaaagc
      541 cggaggaagt ccgggagctt atcgatgcct cgccgtatgc gaaaaagtac acctccggtg
      601 tgctgtcgtt cgcggaggct gaactgccgc ccgggcagcg ggagcagatc atggccagct
      661 ttgagcgggt tctgatgccc gggctcgata aagaccagta cagcattctg tgggtggaac
      721 actccgacaa ggggcggctg gagctgaatt tcctgatccc caacactgaa ctgctgaccg
      781 ggaaacgcct gcagccgtac tacgatcgcg ccgaccggcc gcgcatcgat gcctggcaga
      841 ccgtggtgaa cggcagactg gggctgcatg atccaaacgc cccggagaac cggcggttac
      901 tggtcacgcc gtccgcactg ccggaaacga agatggaagc cgctcaggcg attacaaggg
      961 ggttgctggc cttagcctca tccggggagc taaaaacgcg cgaggacgtc acaggggcgc
     1021 tcacagcggc aggttttgag gtggtccgca ccacaaaaaa cagtatcagc attgccgacc
     1081 cggacggggg gcgaaacatc cgactgaagg gagccattta tgagcagtct tttagcgctg
     1141 gcgaaggact tggagcagca atcgaaagcg cagcagcaga gtaccggcga gatgctgaaa
     1201 gccgcattca gcgagcacga gaagtctgta aaagcggaac tgagcgcaag cgcgaagaga
     1261 atcagcgacg ccatcagcgc ccacgagcag ggtatgaccg cggcgatgca gtcgaaccgc
     1321 ctgagcgtgc tgcgtatggt cggccggacg tggctgacca tcaccctggt ctcggtactg
     1381 ctgatcgcca cgagcgggag cattctgtgg tggcaggggc agcagatcac cgggaattac
     1441 cagactatcc aggctcagga gcggacgcag gccatgctgt cggagaagaa ccacggcgtg
     1501 cagctgtcgc tctgcgggga gaagaaactg tcctgcgtca aagtgaaccc gaaagcggga
     1561 tcttacgggg aggaagggaa ctggatggta ctggagagga aatagcccat gacggaactg
     1621 gaaaaacagt tgctgaccgc attagagcag ctacatcagg actactcgca aaggctggac
     1681 gagtgggaga gcgccttcgc ggaatggcag aagatgtctg gtcttatgca gcgggagaac
     1741 gcggcgctga acgagcgcgt gacgcgcttg agtcagcagg tggcgaactt gagcaggcag
     1801 ttacagcgtt tgagccagta gtgcagcggc atgaaatggc ggtggccgcc gaacgggcat
     1861 acgagcagcg acagcacgaa aaagcgctgg cggaagcccg ttcccgtcag cggagctata
     1921 acggtccgac gctgggctga cagagcttca ccagtgacaa cccccacgaa gaagggctca
     1981 taaatttccc gctaaatacg gtcgtgatga aaacatcacg gcgtatacaa cggagggatt
     2041 atgagacttt ttaacgtgat tgagacggtg ctgaactgcc tgtacatggc gctggagatc
     2101 tgggactggc tggagaagca ttttcctgtt tttaaggcgt tcatccggat tctggcctga
     2161 aatcagaccg tatgcatctg gctgcatgac catgcaacag tgtaatatcc agcggctgcg
     2221 tttctggtgt tttttcgtca tgtctgtcgc cactttctcg cgtctggcac cggaaacgca
     2281 gccaaccccg tttcagcggt gcgggcaacg gatgttatgg taaattgttg tgggttcgac
     2341 agaagttgcc agacggtgta acgttaagac aagaaaaaac gaagccccgg aaatcatgct
     2401 caatcttggc ggatgcacat gataaccagg gccaggtacg aaaccttgaa ggaatcttag
     2461 cacatgaacc gtgccagaca acagcccgcc ataagcgcag gccggattga gtaacgctgc
     2521 actccatctt ttcaatgagc gtttgccgca taagccgtac ttttcggacg atttgcactt
     2581 tggtgtacga attgccggta aagaacgcgc cattctggcg aaatacatcc agttcaatca
     2641 gcctcacgcc atgttctggc tgggattcga cgtcgatcgc gccggtgctg ccattgattg
     2701 gagtgatcgt aatgctccag cgcctacgct gaccatcact aatcctgaca acggacatgc
     2761 gcacctgctg tacgcactaa aaacctcaat ccgcactgcg ccggatggta aaattaaacc
     2821 gctgcgttat gccgccgccg tggagaacgc gctacgtaaa aaattagagg ccgacgcggg
     2881 ctattcggga ctaatctgca aaaatccgaa ccatgcccac tggaaaattg ctgtgtggca
     2941 gccggaactc tacacgctgg actggcttgc tgattttctc gaactaaacg ccgcgaatga
     3001 caaagaaatc attgctgact acggcttagg ccgtaactgc accttgttcg acaagacccg
     3061 gaagtgggca taccgcgcca tccgtcaggg ttggcctgag tatgaacagt ggctgaaagc
     3121 ctgctacgaa cgcgccagtg cttataacct tcaattttca gcgcctcttg acgataaaga
     3181 agtaatgggg attgctaaaa gtattgctaa atggactggc tctaatttta gtaaagaagc
     3241 ttttgatgaa tatgttatac gtactcatac ctctgatata cagagaataa gaggtagaaa
     3301 agggggggct ataggtggaa aaaaaagtaa ttcaggtgga agacctagtt taggtgagcc
     3361 atggattgaa cttggtgtga gtcgacgaac ttactttagg aataaaaaaa ggaaggggtg
     3421 ttaatgcggt taataatttt aatttcatct ttgttactga tcacttcatg cggcgataag
     3481 aataagtcag aatttatgga tggttgcact ggggggagta cgagtgatta cgctcataaa
     3541 gtctgttctt gtgcatatga taaaatgaaa gatacatatg gtgacccgga aaagtgggaa
     3601 tcgaggattt atagagaggg ggcagataac ttgatttcag caatgaaagt gtcaattgaa
     3661 agttgccgat aggaaaaagg ctgctaatgc agcctttttc ctatttcttt aatgaaacat
     3721 aagctataaa aataagaagt agtatcccac caacaatcgc tcctagcgtt gggaaccata
     3781 tgaatggcca aactaaagct ctacctatgt tataggcgac gcctttataa ttaaaatcac
     3841 cccatatact tccgtacacg gcagttccaa cagctaatag tatgtatata ctaaaaattg
     3901 tgctttttat gttcatttaa caattccatt tttaccattg cgtactgtgc atcttgattc
     3961 taacagaact gaatcatcga ccaccctccc atttccttca cttcaaccgc gtggcactaa
     4021 acaaagccta tatcagataa cagccctctc tagcagggcc gcttttttgc agtcagtccg
     4081 cattgaatct gacatactgc actatacctt aagccagtat acactccgct agcgctacgt
     4141 gactggttca gggctgcgcc
//
