LOCUS       NZ_AP022149             4479 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella quasipneumoniae strain WP5-S18-ESBL-05 plasmid
            pWP5-S18-ESBL-05_7, complete sequence.
ACCESSION   NZ_AP022149
VERSION     NZ_AP022149.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194531
            Sequence Read Archive: DRR199367
            Assembly: GCF_014169355.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Klebsiella quasipneumoniae str.
            WP5-S18-ESBL-05 isolated from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4479)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 195x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 10:17:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,866
            Genes (coding)                    :: 5,626
            CDSs (with protein)               :: 5,626
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 79 of 240
            Pseudo Genes (incomplete)         :: 174 of 240
            Pseudo Genes (internal stop)      :: 45 of 240
            Pseudo Genes (multiple problems)  :: 48 of 240
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022149.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WP5-S18-ESBL-05"
                     /isolation_source="wastewater"
                     /db_xref="taxon:1463165"
                     /plasmid="pWP5-S18-ESBL-05_7"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6538 N 139.5126 E"
                     /collection_date="2018-08-06"
     gene            101..421
                     /locus_tag="H7R80_RS29660"
                     /old_locus_tag="WP5S18E05_P70010"
     CDS             101..421
                     /locus_tag="H7R80_RS29660"
                     /old_locus_tag="WP5S18E05_P70010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_024243919.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARAGRLPSI
                     SPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAVLETGGDD
                     DR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            630..>1061
                     /locus_tag="H7R80_RS30115"
                     /pseudo=""
     CDS             630..>1061
                     /locus_tag="H7R80_RS30115"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1099..1584
                     /locus_tag="H7R80_RS29670"
                     /old_locus_tag="WP5S18E05_P70020"
     CDS             1099..1584
                     /locus_tag="H7R80_RS29670"
                     /old_locus_tag="WP5S18E05_P70020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_064181052.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEKSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYQTIQAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGSYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            1588..1800
                     /locus_tag="H7R80_RS29675"
                     /old_locus_tag="WP5S18E05_P70030"
     CDS             1588..1800
                     /locus_tag="H7R80_RS29675"
                     /old_locus_tag="WP5S18E05_P70030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2356..2592
                     /locus_tag="H7R80_RS29680"
     CDS             2356..2592
                     /locus_tag="H7R80_RS29680"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_138134878.1"
                     /translation="MSIIIDREKYTALITLIQTSQGYVGSKVQPNGYTLKCSFDTGFAC
                     CISKNTRNSNLSVHFENESTDPATHFAILSAIS"
                     /name="hypothetical protein"
     gene            complement(2714..3361)
                     /locus_tag="H7R80_RS29685"
                     /old_locus_tag="WP5S18E05_P70050"
     CDS             complement(2714..3361)
                     /locus_tag="H7R80_RS29685"
                     /old_locus_tag="WP5S18E05_P70050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="WP_223811492.1"
                     /translation="MKTIVYVDGFNFYYRKLKDSNKKWLNIKAMVEKLLPDDEIIGINY
                     YTARISGRIDSDAPRRQQIYFNALKSIDNLSIHYGTFSVNESRMFLVQPLDFKHHLKNP
                     NQKIKFANVLKTEEKGSDVNLGCHLVRDGFQGKYEKAVIITNDTDLAEPMRVVKSELNL
                     YIELLSPVNKPARTLSAICDKVTLISDQSIADSQFEDVIVVGEKTVTKPAQW"
                     /name="NYN domain-containing protein"
     Typing          4294..4466
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.5"
                     /coverage="100.0"
                     /evalue="4.4999999999999994e-49"
     Typing          complement(3772..4313)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.809"
                     /coverage="91.0"
                     /evalue="1.5000000000000002e-168"
     Typing          412..1916
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.825"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgacgcgcct gcggcttgtc caacaccgtg ccgcccggga aaaatttccc gcccggcacg
       61 gtgttatcag gaggctactc tggcagacaa acgcaacaag atgctcacga tgtgggtgac
      121 ggaagacgaa caccggcggc tgctcgagcg gtgcgacggc aggcaactgg cggcgtggat
      181 gcgtcagacc tgcctcgatg agaagccggc acgtgccggc agactgcctt cgatatcgcc
      241 tgcgctgctg cgccagcttg ccggtatggg taacaacctg aaccagatcg cccggaaagt
      301 gaatgccggt ggtgccggtc atgatcgggt gcagatcgtg gccgccctga tggcaatcga
      361 cgcgggactg gagcggctgc ggcatgcggt gctggaaaca gggggggatg atgatcgtta
      421 aattccatcc ccgggggcgt ggtggtggtg ccggtccggt ggattacctg ctgggaaaag
      481 accggcagcg cgagggggcg accgtgctgc agggaaagcc ggaggaagtc cgggagctta
      541 tcgatgcctc gccgtatgcg aaaaagtaca cctccggtgt gctgtcgttc gcggaggctg
      601 aactgccgcc cgggcagcgg gagcagatca tggccagctt tgagcgggtt ctgatgcccg
      661 ggctcgataa agaccagtac agcattctgt gggtggaaca ctccgacaag gggcggctgg
      721 agctgaattt cctgatcccc aacactgaac tgctgaccgg gaaacgcctg cagccgtact
      781 acgatcgcgc cgaccggccg cgcatcgatg cctggcagac cgtggtgaac ggcagactgg
      841 ggctgcatga tccaaatgcc ccggagaacc ggcggttact ggtcacgccg tccgcactgc
      901 cggaaacgaa gatggaagcc gctcaggcga ttacacgggg gttgctggcc ttagcctcat
      961 ccggggagct aaaaacgcgc gaggacgtca caggggcgct cacagcggca ggttttgagg
     1021 tggtccgcac cacaaaaaac agtatcagca ttgccgaccc ggacgggggg cgaaacatcc
     1081 gactgaaggg agccatttat gagcagtctt ttagcgctgg cgaaggactt ggagcagcaa
     1141 tcgaaagcgc agcagcagag taccggcgag atgctgaaag ccgcattcag cgagcacgag
     1201 aagtctgtaa aagcggaact gagcgcaagc gcgaagagaa tcagcgacgc catcagcgcc
     1261 cacgagcagg gtatgaccgc ggcgatgcag tcgaaccgcc tgagcgtgct gcgtatggtc
     1321 ggccggacgt ggctgaccat caccctggtc tcggtactgc tgatcgccac gagcgggagc
     1381 attctgtggt ggcaggggca gcagatcacc gggaattacc agactatcca ggctcaggag
     1441 cggacgcagg ccatgctgtc ggagaagaac cacggcgtgc agctgtcgct ctgcggggag
     1501 aagaaactgt cctgcgtcaa agtgaacccg aaagcgggat cttacgggga ggaagggaac
     1561 tggatggtac tggagaggaa atagcccatg acggaactgg aaaaacagtt gctgaccgca
     1621 ttagagcagc tacatcagga ctactcgcaa aggctggacg agtgggagag cgccttcgcg
     1681 gaatggcaga agatgtctgg tcttatgcag cgggagaacg cggcgctgaa cgagcgcgtg
     1741 acgcgcttga gtcagcaggt ggcgaacttg agcaggcagt tacagcgttt gagccagtag
     1801 tgcagcggca tgaaatggcg gtggccgccg aacgggcata cgagcagcga cagcacgaaa
     1861 aagcgctggc ggaagcccgt tcccgtcagc ggagctataa cggtccgacg ctgggctgac
     1921 agagcttcac cagtgacaac ccccacgaag aagggctcat aaatttcccg ctaaatacgg
     1981 tcgtgatgaa aacatcacgg cgtatacaac ggagggatta tgagactttt taacgtgatt
     2041 gagacggtgc tgaactgcct gtacatggcg ctggagatct gggactggct ggagaagcat
     2101 tttcctgttt ttaaggcgtt catccggatt ctggcctgaa atcagaccgt atgcatctgg
     2161 ctgcatgacc atgcaacagt gtaatatcca gcggctgcgt ttttggtgtt ttttcgtcat
     2221 gtctgtcgcc actttctcgc gtctggcacc ggaaacgcag ccaaccccgt ttcagcggtg
     2281 cgggaaacgg atgttatggt aaatagattt attgctgagg tttaatgcct gcagagtaat
     2341 actaatgggg atgtgatgag tattattatt gatcgtgaaa aatatactgc tttgattacg
     2401 ttgatccaga catctcaagg atatgtcggc tcaaaagtac aaccaaatgg atatacattg
     2461 aagtgttctt ttgacactgg ttttgcatgt tgcatctcta aaaatactag aaatagtaac
     2521 ttatctgttc attttgaaaa tgaaagtaca gatcctgcaa ctcactttgc tattttaagt
     2581 gctattagct aattttttta ttttctgcac atagctatag atttcatact ctttatattt
     2641 gtgtgaaggt ataatacaaa acataaaaag cccgggggac gggctttttt tcaacagaac
     2701 gtgtatgtag ctactaccac tgtgctggtt ttgtcacggt tttctcacca actacaataa
     2761 cgtcttcaaa ctggctatca gctatggatt ggtcgcttat aagagtgacc ttatcgcata
     2821 ttgcgctcag ggttcttgct ggcttgttta caggagaaag tagttcaata taaaggttga
     2881 gctctgattt gactactctc attggttcag ccaaatctgt atcattagtt attattacag
     2941 ccttctcgta tttcccttgg aaaccatctc taactaagtg gcaacctaaa ttaacgtcag
     3001 aacccttttc ttctgtcttt aacacgtttg caaactttat tttttgattc gggtttttta
     3061 agtggtgttt gaaatcaaga ggctgcacta aaaacattct gctttcattc accgaaaaag
     3121 taccataatg gatactcagg ttatctatac ttttcagtgc attgaaatat atttgttgcc
     3181 tacgaggtgc gtcagaatca atccttcctg agatcctagc cgtgtaatag ttaattccta
     3241 ttatttcatc atccggtagc aatttttcga ccattgcttt tatgttcagc cactttttgt
     3301 tggaatcttt cagcttacga taataaaagt taaagccatc aacataaact attgttttca
     3361 atggggttag ctccatttta ggtataaaaa aaaccgcatc tgcggttttt ttgcctacgg
     3421 cacgccgtag gagggttgtt tgcctgcggc acgccacagg agggttgttt cttcgcctgc
     3481 ggcacgccac aggggggttg tttcttcgcc tgcggcacgc cacaggtctg taccaacatt
     3541 attgcatttt ttatgtctag tgcaaatgtt ttatatggtg acgaagtaag ccctacctaa
     3601 aggagaaaag atcttcttga gatccttttt ttctgcgcgt aatctcctgc tttgaaaacg
     3661 aaaaaaccac cctggaaggt ggtttttcga aggttagtta atcctggcag attctctaac
     3721 cgcggtaaca gtcttgtgcg agacatgtca ccaaatctgt cctttcagtg tagccgtagt
     3781 caggccacca cttcaagaac tctcgataca tctctcgcac atcctgttta ccagtggccg
     3841 ttgccaatgg cgattagtcg tatctttccg ggttggactc aagttgatag ttaccggata
     3901 agacgcagcg gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg
     3961 ccttcccgga gccgagtgtc aggcgtggaa tgagaaaccg cggccataac agcggaatga
     4021 caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccacca gggggaaacg
     4081 cctggtatct ttatagtcct gtcgggtttc gccaccactg atttgagcgt cagattttgt
     4141 gatgcttgtc aggggggcgg agcctatgga aaaccggcag cgccgggaac cttttcccgt
     4201 tgcctttctt tctctcctgc ccgtatatcg tacatcctgt actttgtgct acttctgctc
     4261 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa tatctcctgt
     4321 attgcatctt ctgctgacgc gccggtgcca cctttttctc ctgccatatg aagcactttc
     4381 ataaagtatt actccgccgt aacatattga gccagtatac actccgctag cgctacgtga
     4441 ctggttcagg gctgcgcccc gaaacccgct aaaagcgac
//
