LOCUS       NZ_AP022148             4862 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella quasipneumoniae strain WP5-S18-ESBL-05 plasmid
            pWP5-S18-ESBL-05_6, complete sequence.
ACCESSION   NZ_AP022148
VERSION     NZ_AP022148.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194531
            Sequence Read Archive: DRR199367
            Assembly: GCF_014169355.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Klebsiella quasipneumoniae str.
            WP5-S18-ESBL-05 isolated from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4862)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 195x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 10:17:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,988
            CDSs (total)                      :: 5,866
            Genes (coding)                    :: 5,626
            CDSs (with protein)               :: 5,626
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 79 of 240
            Pseudo Genes (incomplete)         :: 174 of 240
            Pseudo Genes (internal stop)      :: 45 of 240
            Pseudo Genes (multiple problems)  :: 48 of 240
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022148.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WP5-S18-ESBL-05"
                     /isolation_source="wastewater"
                     /db_xref="taxon:1463165"
                     /plasmid="pWP5-S18-ESBL-05_6"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6538 N 139.5126 E"
                     /collection_date="2018-08-06"
     gene            101..1621
                     /locus_tag="H7R80_RS29640"
                     /old_locus_tag="WP5S18E05_P60010"
     CDS             101..1621
                     /locus_tag="H7R80_RS29640"
                     /old_locus_tag="WP5S18E05_P60010"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005249302.1"
                     /GO_function="GO:0004096 - catalase activity [Evidence
                     IEA]; GO:0020037 - heme binding [Evidence IEA]; GO:0046872
                     - metal ion binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="WP_000076364.1"
                     /translation="MSQDPKKCPVTHLTTEAGAPVVDNQNSMTAGARGPLLAQDLWLNE
                     KLANFVREVIPERRMHAKGSGAFGTFTVTNDITQYTRAKIFSEVGKKTEMFARFSTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDLVGNNTPVFFLRDARKFPDLNKAVKRDPKTNKR
                     SATNNWDFWTLLPEALHQVTIVMSDRGIPAGYRHMHGFGSHTFSFINAQNERFWVKFHM
                     RTQQGIQNLTDAEAADLIAKDRESSQTDLFDAIERGEYPKWKMYVQIMPELEAETVPYH
                     PFDLTKVWPKGDYPLIEVGEFELNRNPENYFQDVEQAAFAPSNLVPGISYSPDRMLQAR
                     LVNYADAARYRVGVNHSQVPVNAARCPVNSNRRDGQGRMDGNYGSLPHYEPNSFNQWQE
                     QPQFKEPALKITGDADFWDFREDDNDYFSQPRALFNLMNDEQKQALFNNTAAAMGDALD
                     FIKYRHIRNCYACDPAYGQGVAKALGMTVADAQAARETDPAKGLPSFL"
                     /name="catalase"
     gene            complement(1747..2715)
                     /locus_tag="H7R80_RS29645"
                     /old_locus_tag="WP5S18E05_P60020"
     CDS             complement(1747..2715)
                     /locus_tag="H7R80_RS29645"
                     /old_locus_tag="WP5S18E05_P60020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="WP_162721734.1"
                     /translation="MKDQITHPPDNTDHSVAKQKFRITNWSTYNKALINRGSLTFWLDD
                     EAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFSLMNVPLR
                     CPDYTSVSKRAKSVNVSFKTSTRGEIAHLVIDSTGLKVFGEGEWKVRKHGKERRRIWRK
                     LHLAVDSNTHEVVCADLSLNNVTDSEAFPGLIRQTHRKIRAAAADGAYDTRLCHDELRR
                     KKISALIPPRKGAGYWPGEYADRNRAVANQRLSGSNARWKWTTEYNRRSIAETAMYRMK
                     QLLGDSLTLRDYDGQVAEAMAMVRALNRMTKAGMPESVRIA"
                     /name="IS5 family transposase"
     gene            complement(2966..3079)
                     /locus_tag="H7R80_RS30210"
     CDS             complement(2966..3079)
                     /locus_tag="H7R80_RS30210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634168.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     gene            3490..3681
                     /locus_tag="H7R80_RS29650"
                     /old_locus_tag="WP5S18E05_P60030"
     CDS             3490..3681
                     /locus_tag="H7R80_RS29650"
                     /old_locus_tag="WP5S18E05_P60030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032421723.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3681..4103
                     /locus_tag="H7R80_RS29655"
                     /old_locus_tag="WP5S18E05_P60040"
     CDS             3681..4103
                     /locus_tag="H7R80_RS29655"
                     /old_locus_tag="WP5S18E05_P60040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_025714834.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTSINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKREPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(2967..3442)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.268"
                     /coverage="92.0"
                     /evalue="0.0"
ORIGIN
        1 cttagtttaa cattttaata agcaagtaaa gggtaagata ggattgaaaa tcttaaattt
       61 ctgcaggaaa tttaaattaa aagaaacggg agttaatagt atgagtcaag accctaaaaa
      121 atgtcctgtt acccacctga ctactgaagc tggtgctcct gtggtcgata atcagaacag
      181 tatgacggca ggtgcgcgtg gacctttact tgcccaagat ttatggttga atgaaaaatt
      241 agcaaacttc gtccgcgaag tgattccaga gcgtcgtatg cacgctaaag gttcaggtgc
      301 ttttggtacc tttaccgtga ccaatgatat tacccaatat actcgcgcta aaattttctc
      361 tgaagtcggc aaaaaaaccg aaatgtttgc gcgtttctca acggtggcgg gtgaacgtgg
      421 cgcggccgat gcagaacgtg atattcgagg ttttgccctg aaattctata ctgaagaagg
      481 caactgggat ctggtcggca ataatacccc ggtgtttttc ctgcgggatg cccgtaagtt
      541 ccctgacctg aataaagcag tcaaacgtga cccgaaaacc aacaagcgca gtgccaccaa
      601 taactgggat ttctggacat tattgcctga agccttgcat caggtgacca ttgtgatgtc
      661 ggatcgcggt attcctgctg gctaccgtca tatgcatggt tttggcagcc ataccttcag
      721 ttttatcaat gcccaaaatg aacgcttctg ggtgaaattc catatgcgta cccagcaagg
      781 catccagaac ctgaccgatg ctgaagcagc agatttgatt gctaaagatc gtgaaagtag
      841 tcagactgac ttatttgatg ccattgagcg tggtgagtat ccaaaatgga aaatgtacgt
      901 tcagatcatg ccggaactgg aagctgaaac agttccttat catccatttg acctgaccaa
      961 agtctggccg aaaggtgatt atccactgat tgaagtcggt gaattcgaac tgaaccgcaa
     1021 tccggaaaat tatttccagg atgtagaaca ggctgccttt gcaccaagca atctggtacc
     1081 gggaattagc tactcaccgg atcgtatgtt acaggcacgt ctggtgaact atgctgatgc
     1141 agcacgttat cgtgttggtg taaatcattc acaggttccg gtcaatgctg cacgctgccc
     1201 tgtaaactct aatcgtcgtg atggtcaggg ccgcatggat ggcaactatg gttcattgcc
     1261 acattatgaa ccgaacagtt ttaaccagtg gcaggaacaa cctcagttta aagaaccagc
     1321 attaaagatt accggtgatg ctgatttctg ggacttccgc gaagatgaca atgactactt
     1381 cagccagccc cgtgccctgt tcaatttgat gaatgatgag cagaaacaag cattgtttaa
     1441 taacacggcc gcagcgatgg gtgatgcgct agatttcatc aaataccgtc atatccgcaa
     1501 ctgttatgct tgcgatccag cttatgggca aggcgttgct aaggccttag gtatgactgt
     1561 agcagatgct caagcggcac gtgaaacaga tcctgctaaa ggtttaccaa gttttcttta
     1621 atgtggaaat ttgatttaga tagttaataa aaaaaccccg acatcctgtc ggggtttttc
     1681 gtatttggta cggctttgtt gaataaataa gatttcgtgc gaacgaccct gtagctggct
     1741 ggattttcag gcaatacgca cgctttctgg catcccggcc tttgtcatcc tgttcaacgc
     1801 acgcaccatg gccatagctt ccgctacctg accatcgtag tcacgcagcg tcagtgaatc
     1861 tcccaacaac tgcttcattc tgtacattgc cgtttccgct atcgagcgac ggttatattc
     1921 cgttgtccat ttccaccgtg cattgcttcc gctcagccgc tgattagcaa cggcacggtt
     1981 gcggtctgcg tactcaccgg gccagtaacc tgctcctttt cggggaggaa taagcgcgct
     2041 gatttttttg cggcgcagtt catcgtgaca gagccgggta tcgtaagccc cgtctgccgc
     2101 ggctgccctg atttttctgt gagtctgccg gataaggccc gggaaggctt ctgagtccgt
     2161 gacgttattc agcgacaggt ctgcacagac aacttcatgt gtgttgctgt caacagcaag
     2221 atgcaacttt cgccagatac gacggcgctc tttgccgtgc tttctgactt tccattcgcc
     2281 ttcaccaaag accttcagcc cggtggaatc aatcaccagg tgtgcgattt caccccgggt
     2341 ggacgttttg aaactgacat taaccgactt tgcccgctta ctgacactgg tgtaatccgg
     2401 gcagcgcaac ggaacgttca tcagggaaaa aatggaatca ataaaaccct gcgcagcccg
     2461 cagggtcagc cggaatacgc gtttaatcac cagaacggtg gtgatggcga gatcagaata
     2521 gcgctggggc cttcctcgtg atgaaggcgt tgccgactca taccaggcct gaatcgcctc
     2581 atcatccagc cagaaagtga gggagccacg gttgatgaga gctttgttgt aggtggacca
     2641 gttggtgatt ctgaactttt gctttgccac ggaatggtct gtgttgtcgg gaggatgcgt
     2701 gatctgatcc ttcaactcag caaaagttcg atttattcaa caaagcccat tatggtgaag
     2761 gatcttcttg agatcctttc tttgccgtcg taatctcttg ctctgtaaac gaaaaaaccg
     2821 cccagcaagg cggttttctc gaaggttctc agagcagcaa ctctttgaac cgaggtaact
     2881 ggctagttag aggagcgcag tcaccaaaac tgttcgttta gtttagcctt aactggcgca
     2941 tgacttcaag actatctcct ctaaatcagt taccagtggc tgctgccagt ggcgcttttg
     3001 catgtctttc cgggttggac tcaagacgat agttaccgga taaggcgcag cagtcggact
     3061 gaacgggggg ttcgtgcata cagtccagct tggagcgaac tgccttcccg gaactgagtg
     3121 tcaggcgtgg aatgagataa acgcggccat aacagcggaa tgacaccggt aaaccgaaag
     3181 gcaggaacag gagagcgcac gagggagcca ccagggggaa acgcctggta tctttaagtc
     3241 ctgtcgggtt tcgccaccgc tgatttgagc gtcagatttt gtgatgcttg tcaggggggc
     3301 ggagcctatg gaaaaacggc aacgccgtgg ccttttccac aggctctttt cttcctggtt
     3361 atcgcgatga ctcttctgtg tgtcccgtcc gctcgccgca gccgaacggc aggagcgtag
     3421 cgactgagtg agcgaggaag cggaaaagag accggtttga cactgagcac tgacgccctg
     3481 aggcccctta tgacgaaaca acagaaaact gccctgaata tggctaaatt catccagaat
     3541 cagtccctgc tcctgctgga gaagcttaac gagcttgatc tcgatgcgga ggctgacctt
     3601 tgtgagaaac tccacgatga tgcggagcat cttttccgta cactgtcctc gagactggat
     3661 gaattacagg atggtaattg atggccagag tgtctattag tgaagcggca cggcttgtta
     3721 aggttagccg tcccacaatt tacaagatga ttaacagtgg gaagttaagt tacacctctg
     3781 ttgtaaagca cgggaaggct attaaagtta ttgatacttc agagcttatt agagtgtttg
     3841 gttctcttga tggtgtcatc gatactgtaa aatatgatgt aaaaagtgac gcagaatcta
     3901 caagtattaa cagcgttggt ttacatgatt tacaacatag aatcgctcta cttgaagctg
     3961 aaaacgatgg tcttaaaggg gctgttaagg ctcgagatga gcatattgat tcccttcgtc
     4021 aggctatgca gcttctggag cacaagcgcg agccttcttc accaccccat tccccgtggt
     4081 ggaaattctg gaaaaaatcc tgatcctgcc atgcttgagt tttcctctcc ggccatccca
     4141 gggagttcct gaaccttccc aaaaatcgcc agatctctga acgctcctca cgactaaaaa
     4201 tacccacaca ctacgcaaaa ttaaaatttt gcagattcct tatttaaaac aattggttaa
     4261 tgaaaaatta atgtgcagtg atgtgttaca aagtgcagat ccaagtcgtg gacgactcta
     4321 tggcgaatcg tttacgaatt gcccacgatt catatgtgaa tcacgcttta ttatcatgtt
     4381 gttatgatga atcgactctt gattcaattc atcaatttgg gcgttttttg ttcatggtga
     4441 ctcttgattc aattcacgat tcgaatcgcc ggtgaatcgt gtggtgagtc gtccggcgac
     4501 ttgccataac gtgattgccg gccgtcgacg aacccgattc ctgaacgtgc tgaaaaccgg
     4561 ttttccggct gcgccggcaa cgcctcacgg ccacgcaata aattgcgtcc ccgttcaccg
     4621 ttttcggatc agaagaacgc cggaaacagg acttaccctg gctgaatggc gtgaatttcc
     4681 gcgctatgca cttgcgcgca tactcatgca tgccgtaaaa acagagcctg cgcgtttctg
     4741 gcgggttttc gggtggtttg ttgcctgttt taccggtttc ccgtcagaaa cgccctgagg
     4801 ccgttttagc ggtgcgcgta agaagggatt atgttaaata aaaagaaatt ttcaaaaata
     4861 tc
//
