LOCUS       NZ_AP022132             2049 bp    DNA     circular CON 14-FEB-2024
DEFINITION  Enterobacter cloacae strain WP5-S18-CRE-02 plasmid
            pWP5-S18-CRE-02_6, complete sequence.
ACCESSION   NZ_AP022132
VERSION     NZ_AP022132.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194526
            Sequence Read Archive: DRR199362
            Assembly: GCF_014169295.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Enterobacter cloacae str.
            WP5-S18-CRE-02 isolated from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 396x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/14/2024 02:27:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,481
            CDSs (total)                      :: 5,366
            Genes (coding)                    :: 5,243
            CDSs (with protein)               :: 5,243
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 45 of 123
            Pseudo Genes (incomplete)         :: 83 of 123
            Pseudo Genes (internal stop)      :: 27 of 123
            Pseudo Genes (multiple problems)  :: 25 of 123
            Pseudo Genes (short protein)      :: 1 of 123
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022132.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="WP5-S18-CRE-02"
                     /isolation_source="wastewater"
                     /db_xref="taxon:550"
                     /plasmid="pWP5-S18-CRE-02_6"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6538 N 139.5126 E"
                     /collection_date="2018-08-06"
     gene            152..1120
                     /gene="mobV"
                     /locus_tag="H7R61_RS27340"
                     /old_locus_tag="WP5S18C02_P60010"
     CDS             152..1120
                     /gene="mobV"
                     /locus_tag="H7R61_RS27340"
                     /old_locus_tag="WP5S18C02_P60010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_232101167.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNADADRTPENHCS
                     VSQSADEAMGKLRELLPEKRRKDAVLAVEYVMTASPEWWNEATPRQQAEFFARSEQWLE
                     NKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMRDDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSQAVNDGFAGTIAMASQSAQNAKRARDLQKTMDSQQKRLQSVTEPFKGLSKEQM
                     TEILMMAQRFKQQNQEREKQQRVERETQRQMRSRGMGGMER"
                     /name="mobV"
     gene            complement(1184..1618)
                     /locus_tag="H7R61_RS27345"
                     /old_locus_tag="WP5S18C02_P60020"
     CDS             complement(1184..1618)
                     /locus_tag="H7R61_RS27345"
                     /old_locus_tag="WP5S18C02_P60020"
                     /inference="COORDINATES: protein motif:HMM:NF017542.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_004193480.1"
                     /translation="MSNRITQIDTATGEEVGSFVAVIRPKQKSAFERHFTMNQDALKIL
                     AKTLTGEQFKVLMMLLADLDYENYIQIAQADIADALGMQKTNVSRSVKALIDLSVILQG
                     PKVGRSYSYRLNPQFGWKGTVSNHKKALKNGLSVIQGGRT"
                     /name="helix-turn-helix domain-containing protein"
     Typing          102..1117
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.215"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgattttgac cttctggcag cttgctgccc gaacgatttt gcgaagcaaa gtctagtgag
       61 tacactatca tcctgatagt ggcccgccgg aggcatcgca atgcgatccc ccgtctggcc
      121 ggatggacgc cggaacgagg gggggggaag catggcgaac tacgcgataa tgcgctgtaa
      181 aaagctgacg ggaatgggca gtgtggccag cgcgttacag cactgctaca gggagagaga
      241 aacgccgaat gctgatgctg accggacacc agaaaaccac tgctctgtgt ctcagtcggc
      301 agatgaggcg atgggaaagc tgcgggagct gttgccggaa aaacgccgta aagatgcggt
      361 actggctgtt gaatacgtta tgacagcaag ccctgagtgg tggaacgagg ccacaccgcg
      421 gcagcaggcg gagtttttcg cccgttctga gcaatggctg gagaacaaat acgggaaaga
      481 tcgcgttgtg gcggcggtgg ttcacaggga cgaggccacc ccgcacctgt ctgcgttcgt
      541 ggtgccattg acgcaggatg ggcggctgtc cgcgaaagag tttattggcg ggcggtcaaa
      601 gatgcgtgac gatcagagca cctacgctga atcggtaaaa aaactgggtc ttgagcgcgg
      661 catcgaggga agccgtgcca cgcatcagac agttcagcac tactacgaat cgataaaccg
      721 tggcacccgt agccaggtat cgatctcacc ggaggcgctg gagccgcgtg tactgcggaa
      781 gggtattttc accaaagacg tggaagatca ggcagccata gccaaacgtt taagccaggc
      841 agtgaacgat ggctttgcag gaaccatcgc tatggcctct cagagcgccc agaatgccaa
      901 aagagcgcgc gatctacaga agactatgga ttcgcagcaa aaacgcctac agagcgttac
      961 agagcctttt aaggggcttt ccaaagagca gatgacagaa atcctgatga tggctcaacg
     1021 gtttaaacag caaaaccaag agagggaaaa acagcagcgt gtcgaacgtg agacacagcg
     1081 acagatgcga tcgcgtggca tgggcggtat ggagcgttag ttttgatgcg atgcaggata
     1141 gggctaacgc cgcaaaatga cgccatttgc ggcattccag agatcaggtt ctgccgccct
     1201 gtataacgct gaggccgttt ttcagtgctt ttttgtggtt gctgacagta cctttccagc
     1261 caaactgagg gttcagacgg taactgtaac tgcgccctac ttttgggcct tgaagaatca
     1321 cggataggtc aattagcgcc ttaaccgaac ggcttacatt ggttttctgc atacctaaag
     1381 cgtcggcaat atcggcttgg gcaatttgga tgtaattttc ataatcgaga tcagctaaca
     1441 gcatcatcaa gactttgaat tgctcccctg tgagggtctt agcaaggatc tttaaagcgt
     1501 cttggttcat agtgaaatgc ctctcaaaag ctgacttctg tttgggtcgg ataactgcga
     1561 caaaactacc tacctcttcc cctgtagcag tgtctatctg agtaattcgg ttactcattt
     1621 aagaggctct cccaaaaaaa tagttatcaa taatgataac aacgttatca ataatgataa
     1681 ctatcgtcaa gcgagagccg cgccccacaa gggctgagag aaggctctct tctgtatctt
     1741 atagcgagaa gctcatatgc ccggatagcg aaccctattt tcatcggttc gtgcgtaagc
     1801 atttctatgc ccgttcaggt gatattccgc ttcctcgttc aggctgttcg ttcctcgctg
     1861 cgctgcggta cgcctcagcc tgacctgcgg cgagcggttc ctgctctttc aaaagcgata
     1921 ttcattgcct gatttcgtca agggcgagcg aagcgagtgc tatttaccct tgacgacact
     1981 ccggataaaa caggcttggc agtggtgggg gtggcgcgga gcgacgcctt ccacctgcca
     2041 gaccgccgt
//
