LOCUS       NZ_AP022131             4666 bp    DNA     circular CON 14-FEB-2024
DEFINITION  Enterobacter cloacae strain WP5-S18-CRE-02 plasmid
            pWP5-S18-CRE-02_5, complete sequence.
ACCESSION   NZ_AP022131
VERSION     NZ_AP022131.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194526
            Sequence Read Archive: DRR199362
            Assembly: GCF_014169295.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Enterobacter cloacae str.
            WP5-S18-CRE-02 isolated from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4666)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 396x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/14/2024 02:27:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,481
            CDSs (total)                      :: 5,366
            Genes (coding)                    :: 5,243
            CDSs (with protein)               :: 5,243
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 45 of 123
            Pseudo Genes (incomplete)         :: 83 of 123
            Pseudo Genes (internal stop)      :: 27 of 123
            Pseudo Genes (multiple problems)  :: 25 of 123
            Pseudo Genes (short protein)      :: 1 of 123
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022131.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="WP5-S18-CRE-02"
                     /isolation_source="wastewater"
                     /db_xref="taxon:550"
                     /plasmid="pWP5-S18-CRE-02_5"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6538 N 139.5126 E"
                     /collection_date="2018-08-06"
     gene            122..445
                     /locus_tag="H7R61_RS27310"
                     /old_locus_tag="WP5S18C02_P50010"
     CDS             122..445
                     /locus_tag="H7R61_RS27310"
                     /old_locus_tag="WP5S18C02_P50010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_007772081.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            654..>1088
                     /locus_tag="H7R61_RS27630"
                     /pseudo=""
     CDS             654..>1088
                     /locus_tag="H7R61_RS27630"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1123..1608
                     /locus_tag="H7R61_RS27320"
                     /old_locus_tag="WP5S18C02_P50020"
     CDS             1123..1608
                     /locus_tag="H7R61_RS27320"
                     /old_locus_tag="WP5S18C02_P50020"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_045326458.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVKAELNESA
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVKVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            1612..1827
                     /locus_tag="H7R61_RS27325"
                     /old_locus_tag="WP5S18C02_P50030"
     CDS             1612..1827
                     /locus_tag="H7R61_RS27325"
                     /old_locus_tag="WP5S18C02_P50030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015589619.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSSD"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2173..2937
                     /locus_tag="H7R61_RS27330"
                     /old_locus_tag="WP5S18C02_P50040"
     CDS             2173..2937
                     /locus_tag="H7R61_RS27330"
                     /old_locus_tag="WP5S18C02_P50040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="WP_045619337.1"
                     /translation="MRIKKPHLRPIVITLSVGGILLTSIFLIAVIMVFQRENIEKDLLD
                     ANSSYAMKMSDVMSGYIEMAQGELAYGAKKIKSTNDIDSLKDEADRLRLQSGMFNSVFV
                     VSNDAVVLATSPESLDLVGTRLNSSASKLAIEEKKPFISSPFKSVTGNLIILLSHPIYD
                     KNGNYIGHVGGSIYLKKHSLFSDVLSRHFFKSDTEISIVSDDGTVIFNNDNSAVGRPMV
                     MSDDLKSKLTESDRGEGEFFFRGIDIFWVMLI"
                     /name="cache domain-containing protein"
     gene            2934..3728
                     /locus_tag="H7R61_RS27635"
                     /old_locus_tag="WP5S18C02_P50050"
     CDS             2934..3728
                     /locus_tag="H7R61_RS27635"
                     /old_locus_tag="WP5S18C02_P50050"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA];
                     GO:0046872 - metal ion binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="WP_048961857.1"
                     /translation="MKNTAWNVLVYSHADSATTILITYAKSVLVLLIVIIITFSLLSYF
                     IASRISLPLEKLAISTNAKDIGASLVYIKAINAWYAEADRLKKALFTHVKVMMERVDSL
                     NEVALKDPLTGVNNRLGFSTKIQNNKYGAGASVIAIDVDFFKKINDSFGHGVGDEVLVS
                     LAQIIKSCCRSEDIICRFGGEEFVVFLPNTSVVTAECVAERIRSVIERTVFPNNLRVTV
                     SAGVATQDDPLGGIDFLLKNADDALYQAKREGRNKVIVYSAI"
                     /name="GGDEF domain-containing protein"
     Typing          4503..4666
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.182"
                     /coverage="95.0"
                     /evalue="9.27e-76"
     Typing          complement(4026..4578)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.639"
                     /coverage="91.0"
                     /evalue="0.0"
     Typing          123..439
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.396"
                     /coverage="99.0"
                     /evalue="1.3e-61"
     Typing          436..1931
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.182"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cccgaaaccc gccaaaagcc tgacgcgcct gcggcttgtc caaccccgcg ccgaccggga
       61 aaggctttcc cgcccgtccc ggcgttatca ggaggccggt ttgtcagaga aacggaacaa
      121 gatgctcacc atgtgggtga cggaggacga gcaccggcgg ctgcttgagc gctgcgacgg
      181 caggcagctg gcagcgtgga tgcggcagac ctgcctggac gaaaagccgg cacgttccgg
      241 aaagcttccc tcaatctcgc cggcgctgct gcgccagctt gccggcatgg gcaataacct
      301 gaaccagata gcccgccgtg ttaacgccgg cggcggtacc ggccatgaca gggtgcagat
      361 tgttgcggcg ctgatggcca tcgatgccgg acttgagcgg ctgcggcacg ccgttcttga
      421 gaaaggaacg gacgatgatc gttaaatttc atccacgcgg tcgcggcggt ggcgccgggc
      481 cggtggacta tctgctgggt aaagaccggc agcgtgaagg cgccaccgtc ctgcagggga
      541 agccggaaga agtccgggag ctgattgacg cgtcgcctta cgttaagaaa tacacctccg
      601 gggtactgtc gtttgcggaa gcggatttac cctccggcca gcgggaaaag ctgatggcaa
      661 gctttgagcg ggttctgatg cccggacttg ataaagacca gtacagcatc ctgtgggtgg
      721 agcatcgtga caaggggcgg cttgagctga attttctgat cccgaacacg gagctgctga
      781 ccggcagacg gctccagccg tattacgatc gcgccgaccg tccacgcatc gatgcctggc
      841 agaccatcgt gaacggcaga ctggggctgc acgacccgaa cgcaccggag aaccggcggg
      901 cactggtcac accgtctgca ctgcctaaag cgaagcagga ggctgcagaa gcgattacac
      961 gcggtctgct tgctcttgcc tcatccgggg agctgaaaac ccgtcaggat gtcactgagg
     1021 cgctggaaag cgcaggtttt gaggtggtcc gcaccacaaa aagcagtatc agcattgccg
     1081 acccggacgg ggggcgaaac atccgactga agggagccat ttatgaacag tcttttaacg
     1141 ctggcgaagg acttagagca gaaatcgaaa gcgcagcagc agagtaccgg cgagatgctg
     1201 aaagccgcat tcagcgagca cgagaaatct gtaaaagcgg aactgaacga aagcgcgaag
     1261 agaatcagcg ccgccatcct cgaccacgac cggaaactgt cctcagccat gagccagcgc
     1321 acgaaaggca tggtgcgcat ggtcagccag acgtggctga ccatcgtcct ggtctccgcg
     1381 ctgctgatcg cctcgagcgc gggcattctg tggtggcagg ggcagcagat actcgacaac
     1441 tacacgacca tccgggagca gaagagcacg caggccatgc tgtcagagag gaacagcggc
     1501 gtgcagctct cgacctgcgg cgagcagaga cgccgctgcg tgaaggtgaa cccggaagcg
     1561 ggacggttcg gagaggattc gagctggatg atactggcgg ggaaatagca catgacggag
     1621 ctggaaaaac agttgctgag cgcattagag cagctacagc aggactactc gaaaaggctg
     1681 gacgagtggg agagcgcctt cgcggaatgg cggacgatgt ctggtcttat gcaacgggag
     1741 aacgcagcgc tgagcgagcg cgtcacgggc ttgagcaggc aggtgcagag tttgagcgag
     1801 cagctgcgcc ggttgtcgag cgactgaatg ccgtcgagct gcaccgggag caggagcgcg
     1861 tggtgcagca tcagaaagcg ctggagctgg agcgatctca gcgacagcgg acgtataaca
     1921 gcccttcact gtgataacct gataaaacgg ggctttcggt ggacatctgc gctgaaagcc
     1981 ggagccattc acccgactgc atagccatgc agccgggtga aatttatctg gcgcagaatg
     2041 cgcggtttag cggagagttt gctgccggtt ttaccgggct gacgccgcat ttgttccgaa
     2101 cccgtccggg gcggtgctga taagggatgt tatggtaaat gcggcatttt ttattatatg
     2161 gagtgtattt ttatgagaat taaaaagcct caccttcgtc cgatagtcat aaccttgtcc
     2221 gttggtggga ttttgttaac ttctattttt ctgattgccg ttattatggt ttttcagcgc
     2281 gaaaatatag aaaaagattt gcttgatgct aattcatcgt acgctatgaa aatgtcagat
     2341 gtaatgagtg gttatattga aatggctcaa ggtgaattag catatggagc aaaaaaaata
     2401 aaatcaacta atgatattga ctcacttaaa gatgaggcgg ataggttaag attgcagtct
     2461 ggtatgttta attcagtttt tgttgttagt aatgatgctg ttgttctggc gacttcgcca
     2521 gaatctcttg atttagttgg tacccgcttg aattcaagtg ccagcaaatt ggcgattgag
     2581 gaaaagaaac cattcatatc ttcaccgttc aaatcagtta ctgggaattt gattattttg
     2641 ttgtcacatc ctatttatga taagaatggc aattacatag ggcatgtcgg tgggtctata
     2701 tatctaaaaa aacatagttt atttagcgat gttttgagca ggcatttttt caagagcgac
     2761 actgaaatta gcattgttag tgatgatgga actgtgatct ttaataacga taacagtgct
     2821 gttggtcggc ccatggttat gtctgatgat ttgaaatcaa agttaacgga gtctgataga
     2881 ggtgaggggg aatttttttt tcggggaata gatatcttct gggttatgct catatgaaaa
     2941 atactgcttg gaatgtgttg gtttatagcc atgctgacag cgcgactact attcttatta
     3001 cttatgctaa aagtgtactt gttctgctaa ttgtaataat tattacattt tcacttcttt
     3061 catatttcat tgcctcccgt atttcacttc ctttagaaaa gcttgctata tctaccaatg
     3121 ctaaagatat aggggcttcg ttagtttata ttaaagctat caatgcttgg tatgccgaag
     3181 ctgatcgact taaaaaggca ctttttacgc atgttaaggt aatgatggaa agggttgatt
     3241 ctttaaatga ggtggcgctt aaagatcccc ttaccggcgt gaacaataga ttggggtttt
     3301 caactaaaat acagaataac aagtatgggg caggagcttc agttattgct attgatgttg
     3361 atttttttaa aaaaataaat gatagtttcg ggcatggggt cggagatgag gtgcttgttt
     3421 ctttggctca gattattaag tcttgttgcc gtagtgaaga tattatttgt cggtttggtg
     3481 gagaagagtt tgttgttttt ctgccgaata catctgtggt aacagcggag tgtgttgcgg
     3541 agcgtattag gagtgttatt gaacgtactg ttttccctaa taacttgcgt gtcaccgtat
     3601 ctgctggtgt ggcaacacaa gatgatcctc ttggtgggat cgattttttg ctgaaaaatg
     3661 ctgacgatgc tctttatcaa gctaagagag aggggcgtaa taaagtgatt gtatatagcg
     3721 ctatttgagt ttttttgatg acgctttacg cgctagtgtg ccgttttttt ttgtggggag
     3781 gggggttcaa gaagcggatc ctcttgttat cccttcgatg tcgtcgtaat ctcatgccct
     3841 gcaaacaaaa aaaccaccgc taccaacggt ggttcgtttg ccggaatcaa gagctaccaa
     3901 ctctttcttc cgaaggtaac tggctttcac ggagcgcagg taccaaaact gtccttccag
     3961 tgtagccgca gtcaggccac cacttcaaga actctgtaga acactctact actccgctcc
     4021 gtgaatcctc ttactaatgg ctaccgccac tggcgatttt tcgtgtctgt tccgggttgg
     4081 actcaagatg atagttaccg ggacaacgtg gtagtcgggc tgaacggggg gttcgtgcat
     4141 acagtccagc ttggagcgaa ctgccttccc ggaaccgagt gtcaggcgtg gaatgagata
     4201 aacgcggcca taacagcgga ttgacaccgg taaaccgaaa ggcaggaaca ggagagcgca
     4261 cgagggagcc accaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     4321 actgatttga gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta tggaaaaacg
     4381 gcttcgccgt ggccttatcg cttccctgtt aagcttcttc ctggcatctc ccaggaaatt
     4441 tccgccccgc ctgtaagccg gtaccgctcg ccgcagccga acgaccgagc gcagcgagtc
     4501 agtgagcgag gaagcggaat atatcctgta tcacatattc tgctgacgct ccgctgctgc
     4561 atttttctcc tgccacatga agcacttcac tgacactcgc atccgtgcca acatagtaag
     4621 ccagtataca ctccgctagc gctacgtgac tggttcaggg ctgcgc
//
