LOCUS       NZ_AP022130             4844 bp    DNA     circular CON 14-FEB-2024
DEFINITION  Enterobacter cloacae strain WP5-S18-CRE-02 plasmid
            pWP5-S18-CRE-02_4, complete sequence.
ACCESSION   NZ_AP022130
VERSION     NZ_AP022130.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194526
            Sequence Read Archive: DRR199362
            Assembly: GCF_014169295.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Enterobacter cloacae str.
            WP5-S18-CRE-02 isolated from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4844)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 396x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/14/2024 02:27:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,481
            CDSs (total)                      :: 5,366
            Genes (coding)                    :: 5,243
            CDSs (with protein)               :: 5,243
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 45 of 123
            Pseudo Genes (incomplete)         :: 83 of 123
            Pseudo Genes (internal stop)      :: 27 of 123
            Pseudo Genes (multiple problems)  :: 25 of 123
            Pseudo Genes (short protein)      :: 1 of 123
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022130.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4844
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="WP5-S18-CRE-02"
                     /isolation_source="wastewater"
                     /db_xref="taxon:550"
                     /plasmid="pWP5-S18-CRE-02_4"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6538 N 139.5126 E"
                     /collection_date="2018-08-06"
     gene            101..427
                     /locus_tag="H7R61_RS27275"
                     /old_locus_tag="WP5S18C02_P40010"
     CDS             101..427
                     /locus_tag="H7R61_RS27275"
                     /old_locus_tag="WP5S18C02_P40010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_042014476.1"
                     /translation="MEKRTKEIKIRLTETEHQRLLDRCDRTHLAEWLRQLGLGEHISRK
                     RRVPDVAPELLRQVSGIGNNLNQIARRLNQTDSLTPAERASLLVVLTSLDRQLGDLLEQ
                     NRDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            636..>1058
                     /locus_tag="H7R61_RS27620"
                     /pseudo=""
     CDS             636..>1058
                     /locus_tag="H7R61_RS27620"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1105..1590
                     /locus_tag="H7R61_RS27625"
                     /old_locus_tag="WP5S18C02_P40020"
     CDS             1105..1590
                     /locus_tag="H7R61_RS27625"
                     /old_locus_tag="WP5S18C02_P40020"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_221697229.1"
                     /translation="MSEILNLAKGFEEKSKQQAQDTEQSVKREFARLETSISAELQSSS
                     QSISNAIREQNGRLTGMIRTSVSTAMAWVFLCFALLIGILSGIIWFQGTIITSRLSEMD
                     SYSQQLANLKAKSGAGVTIYSDDTRRNTYLVVLPKQAHGVETYTSTTDNTVVKYTAK"
                     /name="MbeB family mobilization protein"
     gene            1660..1884
                     /locus_tag="H7R61_RS27285"
                     /old_locus_tag="WP5S18C02_P40030"
     CDS             1660..1884
                     /locus_tag="H7R61_RS27285"
                     /old_locus_tag="WP5S18C02_P40030"
                     /inference="COORDINATES: protein motif:HMM:NF016769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_232101166.1"
                     /translation="MEKQLLSALESLQTGYDQQQQAWQDSYSSLQHMFEATSQALTHSD
                     RVCQHLSSQVESLRAQVESLSRNVSRLMR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2017..2454
                     /locus_tag="H7R61_RS27290"
                     /old_locus_tag="WP5S18C02_P40040"
     CDS             2017..2454
                     /locus_tag="H7R61_RS27290"
                     /old_locus_tag="WP5S18C02_P40040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005555990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_047590544.1"
                     /translation="MTTYWHMQMHPDDKSFADTHIYSILESKKIIGLGEWNEGESIISA
                     FKNDLKVNDIVAIKNGAKLIALVQVIGGAYAVHDDSSEIGWIEHRRPIRVLDWEIWGKT
                     LPQPRGTLVKCVNEVETTKIIREWHTRISTVLTKRGIATSV"
                     /name="hypothetical protein"
     gene            2545..2880
                     /locus_tag="H7R61_RS27295"
                     /old_locus_tag="WP5S18C02_P40050"
     CDS             2545..2880
                     /locus_tag="H7R61_RS27295"
                     /old_locus_tag="WP5S18C02_P40050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012442911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_114261837.1"
                     /translation="MSHAIEFIETPMFTRQIKQIATDEELRELQKTLIESPDKGDLIQK
                     TGGLRKVRMATGNQGKSGSARVIYFLATAEVIYLVMAYPKSTKDSLTDAEKAALRKLTK
                     QLNTEEY"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2896..3180
                     /gene="nadS"
                     /locus_tag="H7R61_RS27300"
                     /old_locus_tag="WP5S18C02_P40060"
     CDS             2896..3180
                     /gene="nadS"
                     /locus_tag="H7R61_RS27300"
                     /old_locus_tag="WP5S18C02_P40060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_001697687.1"
                     /translation="MSFFDELKASLEEAVEIKNGVREPARVTRYELADVKAIRAQLNVS
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFQELAAH"
                     /name="nadS"
     gene            3374..4276
                     /locus_tag="H7R61_RS27305"
                     /old_locus_tag="WP5S18C02_P40070"
     CDS             3374..4276
                     /locus_tag="H7R61_RS27305"
                     /old_locus_tag="WP5S18C02_P40070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_182945093.1"
                     /translation="MHNAALALFNDRLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRAGAAIDWSDRNAPAPTLTITNPDNGHAHLLYALKTSIRTAPDGKMK
                     PLRYAAAVENALRKKLEADAGYSGLICKNPNHGHWKIAVWQPELYTLDWLADFLDLSAA
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYDQWLLACYERASAYNLQFASPLD
                     EKEVNGIAKSIAKWTHHQLSPEGFAEYVARTHAPSIQAVRGRKSRGGGRPKDSSALVNT
                     KPWLSLGISRATFYRKQGG"
                     /name="replication initiation protein"
     Typing          418..1976
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.678"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gccaccgtga ctggttcagg gggctcgccg ccccccaaaa cccccggcat ccactgcgaa
       61 aaattctcac ggtggtgcga attttttctc agcggagcct atggaaaagc gaacgaaaga
      121 gatcaaaatc cggctcaccg aaactgagca tcagcgattg cttgatcgct gtgaccgtac
      181 tcaccttgcc gaatggctac gtcagctcgg tctgggtgaa cacatctcac gcaagcgccg
      241 tgtgcctgac gtggcgcccg aactgctgcg gcaggtcagc gggattggca ataacctgaa
      301 ccagatcgcc cgtcgcctga atcagactga ttccctgacg cctgctgaac gtgcctcgct
      361 gctcgtcgta ctgaccagcc ttgaccgtca gcttggcgac ctgctggagc aaaaccgtga
      421 tcgttaaaat tcactcgcgg ggtgcgggtt cgggtagcgg tcctgtggat tacctgctgg
      481 gaaaagaccg gaagcgcgaa caggccagcg tactgcgagg caatcctgaa catgtgcgcg
      541 agctgatcga cggctgcgac tttgcccgcg cctacacttc cggcgtgctt tcattccagg
      601 aacctgacat tgcagacgcg gagaaatcgc gcctgatgga cgaatgggag cacacgctgc
      661 tgaccggcct cgaccgcgac cagtacgcct gcctgtgggt ggagcaccgc gacaagggac
      721 ggcttgagct gaattttgtc atcccgaaca tcgaattgca gagtggaaaa cgcctgcaac
      781 cctactttga ccgcgccgac cgcccccgcg tcaacgcctg gcagaccctg accaatgacc
      841 ggctggggct gcgcgacccg aatgacccga cgtaccgccg tccgctgacg caggccagcg
      901 atctaccccg cgacaaacag caggcggcag agaaaatcac cgccggcctg atgaacctga
      961 tgcagcaggg ggtgatccgc agccggcagg acgtggtgac gcagctggaa agctacggtc
     1021 tgaccgtggc gcgggaaacg aaaagcagca tcagcattgc cgacccggac ggcggccgaa
     1081 atatccggct gaaaggaatg atatatgagc gagattttaa atttggcgaa gggcttcgag
     1141 gagaaatcga agcagcaggc gcaggatacc gagcagagcg tgaagcgcga gttcgcgagg
     1201 ctggagacgt ctatcagcgc ggaattgcaa tcaagctcgc agagcatcag caacgctatc
     1261 cgcgagcaga acggcaggct gacgggcatg atcagaacct ccgtttcaac ggcgatggcg
     1321 tgggtattcc tctgcttcgc cctgctgatc ggcatcctga gcgggataat ctggtttcag
     1381 ggcacgatca tcacctcacg cctgagcgag atggacagct acagccagca gctggcgaac
     1441 ctgaaagcga aaagcggcgc gggcgtgacg atttacagcg acgacacgcg gcggaacact
     1501 tacctggtgg tgctgccgaa gcaggcgcac ggggtggaaa cctacacgtc gacgaccgac
     1561 aacaccgtgg tgaaatacac ggcaaaataa ccccgccaga gcgccctgaa acgccacagc
     1621 gcgtttttaa cactgaggac gaccaaaacc atgaccgaga tggaaaaaca gcttctgagc
     1681 gccttagaga gcttacagac aggctacgat caacagcagc aggcgtggca ggacagctac
     1741 agcagtttgc agcacatgtt cgaggctact tcacaggcgc tgacgcacag cgaccgggtg
     1801 tgtcagcact tgagcagtca ggtagagagc ttgcgcgcgc aggtcgagag cttgagcagg
     1861 aacgtcagcc gcttgatgcg ctgatcgtgc agcatgaccg cgcccgtgca gagaagctgc
     1921 gcgagcagca accggagccg cagaaaaagc cgacccgcta ttacggtccc tcgatgtaat
     1981 acgggatatt catataaaaa aatcaaagga gaaaaaatga ctacctactg gcatatgcag
     2041 atgcaccctg acgataagtc attcgccgat actcatatat attcaatcct ggaaagtaag
     2101 aaaattattg ggttaggtga gtggaacgaa ggtgagtcga taatctcagc tttcaaaaat
     2161 gacctgaaag taaatgatat tgttgcaatc aaaaatggag caaagctgat cgcccttgtt
     2221 caggttattg gcggagcata cgccgtacat gatgacagca gcgaaattgg ctggattgaa
     2281 catcgtcgcc ctattcgagt tctcgactgg gagatctggg gtaaaacatt acctcagccc
     2341 agaggtactc ttgtgaaatg tgtgaatgag gttgaaacaa ccaaaattat cagagagtgg
     2401 cacacacgca tttccactgt gctgaccaag agggggattg ctacttcagt ctgacatcaa
     2461 cgttgcaacc gacactggaa atagaaactt taaaaaacag ccccaatagt gtaccttgta
     2521 cacaagtaag agcaggagac agccgtgagt cacgcgattg agtttatcga gaccccgatg
     2581 tttacccgtc agataaaaca gatcgccacg gatgaagaac tcagggagct gcaaaaaacg
     2641 ctgattgagt caccggacaa aggcgatctt atccagaaaa ccggcgggct gcgaaaagtc
     2701 aggatggcga cgggcaacca ggggaaaagc ggcagcgcga gggtgattta cttcctggcc
     2761 accgccgagg tgatttatct ggtgatggcc tatccgaaaa gcaccaaaga cagcctgacg
     2821 gacgcggaga aagccgcact gagaaagctg acgaaacaac tgaatactga agaatactga
     2881 aagacgaggt gtgacatgag tttttttgac gagctgaaag catcgctgga agaagccgtt
     2941 gaaatcaaaa acggcgtcag agagcctgcc cgcgtcaccc gttacgagct ggcagacgtg
     3001 aaggccatca gggcacagct gaacgtttca cagagcgaga tggcgaaagt gctgggaacc
     3061 agtctcgata ccatcaagag ctgggaaacc ggacgacgta acccgaccgg gctggcagca
     3121 aaggtgctgg caaccatcca ggcgaacccg aaatttttcc aggagctggc ggcgcactaa
     3181 gccgttgtga cagaaggatt cgacaggcga ggccgttcgc gtgtaattta gcggcaacag
     3241 aaaaaacaaa gccccggaaa tcatgctcaa tcttggcgga tgcacatgat aaccagggcc
     3301 aggtacgaaa ccttgaaagg atattagcac atgaaccgtg ccagacaaca gcccgccata
     3361 agcgcagggc gcattgcata acgccgcact cgcactcttt aacgaccgct taccgcacaa
     3421 gccgtacttt tcggacgatc tgcactttgg tgtacgcatt gccggtaagg aacgcgccat
     3481 tctggcgaaa tacattcagt tcaatcagcc tcatgccatg ttctggctgg gattcgatgt
     3541 cgatcgcgcc ggtgctgcta tcgactggag cgaccgcaac gctccggcac ccacgctgac
     3601 catcaccaat cccgacaacg gacacgcgca cctgctgtac gcgttaaaaa cctcgatacg
     3661 caccgcgccg gacggaaaaa tgaagccgct gcgctacgct gccgccgtgg agaacgcgct
     3721 acgcaaaaaa ttagaggctg acgcggggta ttcggggtta atctgcaaaa atccgaacca
     3781 cggtcactgg aaaattgccg tgtggcagcc ggaactctac acgctggact ggctggccga
     3841 ctttctcgac ctgagtgcgg cgaacgacaa ggaaattgtc gccgactacg gcttaggccg
     3901 aaactgcacc ctgttcgaca agacccgcaa atgggcatat cgagccatac gtcagggctg
     3961 gcccgagtat gaccagtggc tgctagcctg ctacgaacgt gccagtgcct acaatctgca
     4021 attcgcatca cctctggacg aaaaagaggt gaacgggata gcaaaaagta ttgctaaatg
     4081 gacacatcac caactatccc ctgaaggatt tgcggagtat gtagcacgta cgcacgcacc
     4141 atctattcag gctgttagag gccggaaaag cagaggcggg ggcaggccaa aggacagttc
     4201 agccctcgta aacacgaagc cttggctgtc ccttggtatt agccgggcaa ctttctatcg
     4261 caagcaggga ggttagtttt taaactctcg agctatagcg aacaaagtca ctaaaatcgc
     4321 aaaaatcact attaagttca tgacaggcaa ggagcaaaga gccataacca actgtaaaag
     4381 tagatttcca acgattcttg caatgataaa actacaaacg taatcactaa tcaaatcaaa
     4441 catatatatc tccacgaagg agagtccatg cgataaatat ccaaggggga acgtatccct
     4501 tgtgtccgaa ctatgcttga cgttttttac ctatttgcta atatacgttt accaagacga
     4561 taaaagcgaa agatacaaaa caaaaccagc ccttcattta accataaaaa ctccaaaaag
     4621 ttaaagtccc aattataaca ataacacata ttttcggtaa agcaaatatt tttttcactt
     4681 ttcgcaaaaa aaattactca tctcctttta tgcgtgagac aaaaacaacc tatatcagat
     4741 aacagctccc tttcagtgac ccctgccttc ttgaactcaa cacagaacct gacatactgc
     4801 atcatacttt aagccagtac acacagcgtc ccctctaacg aggt
//
