LOCUS       NZ_AP022123             7233 bp    DNA     circular CON 14-JUN-2023
DEFINITION  Escherichia coli strain WP5-W18-ESBL-11 plasmid pWP5-W18-ESBL-11_3,
            complete sequence.
ACCESSION   NZ_AP022123
VERSION     NZ_AP022123.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194523
            Sequence Read Archive: DRR199359
            Assembly: GCF_014169275.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Escherichia coli str. WP5-W18-ESBL-11
            isolated from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7233)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 241x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/14/2023 05:26:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,879
            CDSs (total)                      :: 4,763
            Genes (coding)                    :: 4,580
            CDSs (with protein)               :: 4,580
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 183
            CDSs (without protein)            :: 183
            Pseudo Genes (ambiguous residues) :: 0 of 183
            Pseudo Genes (frameshifted)       :: 77 of 183
            Pseudo Genes (incomplete)         :: 106 of 183
            Pseudo Genes (internal stop)      :: 42 of 183
            Pseudo Genes (multiple problems)  :: 38 of 183
            Pseudo Genes (short protein)      :: 2 of 183
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022123.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="WP5-W18-ESBL-11"
                     /isolation_source="wastewater"
                     /db_xref="taxon:562"
                     /plasmid="pWP5-W18-ESBL-11_3"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6538 N 139.5126 E"
                     /collection_date="2018-02-13"
     gene            complement(101..325)
                     /locus_tag="H7R04_RS24030"
                     /old_locus_tag="WP5W18E11_P30010"
     CDS             complement(101..325)
                     /locus_tag="H7R04_RS24030"
                     /old_locus_tag="WP5W18E11_P30010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_089537792.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENLLTSPSGRVSRF"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            389..712
                     /locus_tag="H7R04_RS24035"
                     /old_locus_tag="WP5W18E11_P30020"
     CDS             389..712
                     /locus_tag="H7R04_RS24035"
                     /old_locus_tag="WP5W18E11_P30020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001430281.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            921..>1352
                     /locus_tag="H7R04_RS24505"
                     /pseudo=""
     CDS             921..>1352
                     /locus_tag="H7R04_RS24505"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1390..1908
                     /locus_tag="H7R04_RS24045"
                     /old_locus_tag="WP5W18E11_P30030"
     CDS             1390..1908
                     /locus_tag="H7R04_RS24045"
                     /old_locus_tag="WP5W18E11_P30030"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001749510.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQTNLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            1915..2148
                     /locus_tag="H7R04_RS24050"
                     /old_locus_tag="WP5W18E11_P30040"
     CDS             1915..2148
                     /locus_tag="H7R04_RS24050"
                     /old_locus_tag="WP5W18E11_P30040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_004026237.1"
                     /translation="MTELEIHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2528..2842)
                     /locus_tag="H7R04_RS24055"
                     /old_locus_tag="WP5W18E11_P30060"
     CDS             complement(2528..2842)
                     /locus_tag="H7R04_RS24055"
                     /old_locus_tag="WP5W18E11_P30060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024139128.1"
                     /translation="MSNNYDLIAKAIKEKLQVTAYYQGFYREMCPHALGSKKGRKQALF
                     YQFGGESSKGTVTPGSTFNWRCIPIDGLTEVTLQSGQWYTAENHSQAQTCVDQIDIEVQ
                     "
                     /name="hypothetical protein"
     gene            complement(2934..3236)
                     /locus_tag="H7R04_RS24060"
                     /old_locus_tag="WP5W18E11_P30070"
     CDS             complement(2934..3236)
                     /locus_tag="H7R04_RS24060"
                     /old_locus_tag="WP5W18E11_P30070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024139129.1"
                     /translation="MLKTVRSCLLSALLTHLFFGNIAVAKENLSDDKVKQAIIDESIAA
                     YPGTCACPFNSARNGSSCGRRSAWSKAGGYSPICYKKEVTKEMIKDWRASHSNNN"
                     /name="hypothetical protein"
     gene            complement(3336..3764)
                     /locus_tag="H7R04_RS24065"
                     /old_locus_tag="WP5W18E11_P30080"
     CDS             complement(3336..3764)
                     /locus_tag="H7R04_RS24065"
                     /old_locus_tag="WP5W18E11_P30080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001749513.1"
                     /translation="MKRSGGTRYLYLISLITVAAALTACTPKGSVEQHTRHYVYASDDR
                     SDPNFYTNKADTTRMMIPFFQQFREMGEKDKAAGVSAETAQQRIKEFHSEKFLQSLRST
                     TTFAGRKYMNSDMPSPEKMKLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(3845..4282)
                     /locus_tag="H7R04_RS24070"
                     /old_locus_tag="WP5W18E11_P30090"
     CDS             complement(3845..4282)
                     /locus_tag="H7R04_RS24070"
                     /old_locus_tag="WP5W18E11_P30090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001749514.1"
                     /translation="MGHCNFMAWHTLRDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRR
                     EFETSELIRAYGELKQNETQERHTVGQAENPHDQQTERILRELNELKQYLTLMLEDKQA
                     QDMDRRHQEAEREQLQNEIAQLRQALEQEKKRGFWSRLFGH"
                     /name="hypothetical protein"
     gene            4542..5705
                     /gene="cna"
                     /locus_tag="H7R04_RS24075"
                     /old_locus_tag="WP5W18E11_P30100"
     CDS             4542..5705
                     /gene="cna"
                     /locus_tag="H7R04_RS24075"
                     /old_locus_tag="WP5W18E11_P30100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin colicin N"
                     /protein_id="WP_040089861.1"
                     /translation="MGSNGADNAHNNAFGGGKNPGIGNTSGAGSNGSASSNRGNSNGWS
                     WSNKPHKNDGFHSDGSYHITFHGDNNSKPKPGGNSGNRGNNGDGSSAKAGEITITPDNS
                     KPGRYISSNPEYSLLAKLIDAESIKGTEVYTFHTRKGQYVKVTVPDSNIDKMRVDYVNW
                     KGPKYNNKLVKRFVSQFLLFRKEEKEKNEKEALLKASELVSGMGDKLGEYLGEKYKNVA
                     KEVAGDIKNFHGRNIRSYNEAMASLNKVLANPKMKVNKSDKDAIVNAWKQVNAQDMANK
                     IGNLGKAFKVADLAIKVEKIREKSIEGYNTGNWGPLLLEVESWIIGGVVAGVAISLFGA
                     VLSFLPISGLAVTVLGVIGIMTISYLSSFIDANRVSNINNIISSVIR"
                     /name="cna"
     gene            complement(5714..6238)
                     /gene="cni"
                     /locus_tag="H7R04_RS24080"
                     /old_locus_tag="WP5W18E11_P30110"
     CDS             complement(5714..6238)
                     /gene="cni"
                     /locus_tag="H7R04_RS24080"
                     /old_locus_tag="WP5W18E11_P30110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_061352107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin N immunity protein"
                     /protein_id="WP_040089860.1"
                     /translation="MDIKDRNKISKKISFSLLLLLSPFALIFFSYNNAPIPLLEKIIAY
                     LSLPGFHSSNNPPLSEAFNLYVHTAPLAAISLFIFTHKELELKPKSSSLRALKILTPFT
                     ILYISMIYCFLLTDTELTLSSKTFVLMSKNDLFLSFFYITLYIGIYIFTYLYLWFFIGT
                     YKLFTRGGILP"
                     /name="cni"
     gene            6266..6403
                     /locus_tag="H7R04_RS24085"
     CDS             6266..6403
                     /locus_tag="H7R04_RS24085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329514.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_024139133.1"
                     /translation="MCGKILLILFFIMTLSACQVNHIRDVKGGTVAPSSSSRLTGVEIK
                     "
                     /name="colicin release lysis protein"
     Typing          18..105
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          complement(6682..7227)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.536"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          703..2252
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.565"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggcgtgcttt tcatacaatc cgcactgatg ccgcatagtt aagccagtat acactccgct
       61 atcgctacgt gactgggtca gggctgcgcc ccgacacccg ctaaaacctg ctgacgcgcc
      121 ctgacgggct tgtcagcagg ttttcaccgt catccccgaa acgtgcgagg cagctgcggt
      181 aaagctcatc ggcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca
      241 gttcgttgag cttttccagc agcgttaatg tctggcttct gataaagcgc gccatgttaa
      301 gggctgtttt ttcctgttta gtcactgatg cctccgtgta agggggattt ctgttcatgg
      361 ggtaatgata ccgatgaaac gcgagaggat gctcacgata cgggttactg atgatgaaca
      421 tgcccggtta cttgaacgct gtgagggtaa acaactggcg gtatggatgc ggcgggtctg
      481 cctgggggag ccggttgccc gttccgggag gctgccgaca ctggcaccgc cgttactgcg
      541 tcagctggcc gccatcggaa ataacctgaa tcagacagcc cgtaaggtga acagcgggca
      601 gtggtcttcc ggtgaccggg ttcaggtggt ggccgcactg atggccatcg gggatgagct
      661 gcgccggctg cgtctggccg tcagggaaca gggggcgcgg gatgatagtt aaatttcatg
      721 ccaggggaaa aggtggtggc agtggtccgg ttgattacct gctggggagg gagcgtaacc
      781 gcgaaggcgc aacggtgctt cagggtaatc cggaagaagt ccgggaactc atcgatgcca
      841 cgccatttgc gaagaaatac acgtccggtg ttctgtcgtt cgcggagaag gaactgccgc
      901 cgggaggacg tgaaaaagtg atggcgagct ttgagcgtgt actgatgccc ggtctcgaaa
      961 aaaatcagta cagcatcctg tgggtggagc accaggacaa gggacggctt gagctgaatt
     1021 ttgtcattcc gaacatggag ctacagaccg gaaaacgtct ccagccgtac tacgaccgcg
     1081 cagacaggcc gagaattgat gcctggcaga cgctggtgaa tcaccattac ggactgcatg
     1141 accccaacgc cccggagaac cgccggacgc tgacccttcc tgataacctg ccggaaacga
     1201 aacaggcact tgctgagagc gttacacgcg gtatagacgc actttaccat gccggagaga
     1261 taaaaggccg tcaggatgtg attcaggcgc tcacagaggc ggggcttgaa gtggtcaggg
     1321 tgacgcgaag cagtatcagc attgcagacc cgaacggcgg gaggaatatc aggctgaaag
     1381 gagcatttta tgagcaatct tttacagacg ggcgcggagt tcgagaaaaa gctgaaagag
     1441 agagccgaat ctacagagaa aatgctgaac gacgagttca ggaagctcgg cgagtctgtc
     1501 agcgaggctg tgacctcaaa cgagacgaaa atcagagacg ctatagccct gttcacagcc
     1561 tcgacagaga aatcgctgga aaaacaccgg gaaggggtga aagaggcgat gatgcagcac
     1621 aggaaggacg tgttaaagct ggcagggaat acgggcatga tgttactggg gatagtcttt
     1681 ctcctgttta ccgcgagtgg cgggacgctc tggtatcttg gcggacggat acagacgaac
     1741 ctggaagaaa tcaggaagca ggaagagaca ttgcagaaac tgaacgcgaa gacatggggc
     1801 gtggagtttg tgcaggacgg gaacaggaaa ttccttgtcc ttccgtacgg gaaatcagcg
     1861 gaggtgattc cctttcaggg gaaagagtgg gtacatctga aggagtgaca cagagtgaca
     1921 gagctggaaa tacatttgct gaacgcctta gagcagctgc aacaggacta tatgcagcgg
     1981 ctgagcgaat gggagagcgc cttcgtggaa ttgcagaaga tgttttcgct tacgcaacgg
     2041 gacaacgcga tgctgaacga gcgggtcatg cagttgagtc agcaggtgca gcacttgagc
     2101 gagcagacag aacgcttgag ccagttatac agcgagaact ggagataaga gaggaacggt
     2161 tgatacagga gcgcgaacat gtgttatccc tggaacggga gcgtcagccg gaaatacagg
     2221 aacgcacgct ggatggccct tcgctgggat ggtgaaacca tgaaaaatgg cagcttcagt
     2281 ggattaagtg gggggaatgt ggcctgtacc atctggttgc ataggtattc atacggttaa
     2341 aatttatcag gcgcgatcgc ggcagttttt cgggtggttt gttgccattt ttacctgttt
     2401 actgccgtga tcgcgctgaa cgcgttttag cggtgcgtac aatcagggct ttatgttaaa
     2461 tgctgatggt tgtcataaac ataaagctgc cactaatggc agctttatga gggtacgaat
     2521 acaggcttta ttgaacttca atatcgatct gatcaacaca agtctgtgct tggctgtgat
     2581 tttctgctgt gtaccactgg cctgattgta aggtgacctc agttagacca tctataggaa
     2641 tgcagcgcca gttgaatgtg gaaccaggtg tcacagtacc tttactgctt tcaccgccaa
     2701 actgatagaa aagagcctgt tttcgtcctt tttttgaacc tagcgcatga gggcacatct
     2761 cgcggtaaaa accttggtaa tatgctgtta cttgcaactt ttctttgatt gccttggcaa
     2821 ttaaatcata attattagac acttagcttc aatctcccag agtatgattt cctgttggaa
     2881 atatatgtat ttatcagaaa tgtaatgtaa acagaaagtt ataaaaatag aagtcagttg
     2941 ttatttgagt gagaagctcg ccaatctttt atcatttctt ttgttacctc ttttttgtag
     3001 cagattggcg aataacctcc tgctttgctc caggcgctgc gcctgccgca cgagctgcca
     3061 tttcttgcgc tgttaaacgg acatgcacat gttccgggat aggctgctat cgactcgtca
     3121 ataatcgcct gctttacttt gtcatctgat agattttctt tcgccacagc gatattgccg
     3181 aaaaaaagat gtgtcagtaa agctgataac agacacgaac gtactgtctt aagcatttga
     3241 atagtctccg aggaataatc ctaggagaat tgcatttttg tacaaaaaaa caacttaaat
     3301 cgcttcttgt tgttaaggga ttatggtaaa tccacttact gtctgccctc gtagccatcg
     3361 agataaaccg cagaaatcgt gtcagccagc agcttcattt tttccggtga tggcatatcg
     3421 ctattcatgt attttctgcc cgcaaaggta gttgtgcttc tgagagactg aagaaatttt
     3481 tctgagtgga attcttttat gcgttgctgt gccgtttctg ctgatactcc ggctgctttg
     3541 tctttctccc ccatctccct gaactgctga aagaacggaa tcatcatccg tgtggtgtcg
     3601 gccttgttgg tgtagaagtt agggtcagaa cggtcatcgg aagcgtacac ataatgtcgg
     3661 gtatgttgtt ccacactgcc tttcggggta caggccgtga gggctgcagc cacagttatc
     3721 agggatataa gatacagata gcgtgtgcca ccagaacgtt tcatacgtgt ctccttacct
     3781 taaagtattt attctcggtg agttattatc cgaagactat cttatcatca gcctctgacc
     3841 gagttcagtg accgaagagt ctggaccaga atccccgttt cttttcctgt tccagtgcct
     3901 gcctgagctg ggctatctca ttctgtaact gttcccgttc tgcttcctgg tgcctgcgat
     3961 ccatatcctg tgcctgttta tcctcaagca tcagcgtcag gtattgtttc agctcattca
     4021 gttcccggag aatgcgttct gtctgctgat catgtggatt ttctgcctgt cccacagtgt
     4081 gcctttcttg tgtctcattt tgctttaatt cgccgtatgc ccggatcagt tcactggttt
     4141 caaactccct tcgtccgtcc tggctggtac ggtaactgac aagacctgag cgcatatcac
     4201 ggtaaaactg tgaacgcgat cttccagtca ttttcagtgc gtcccggagt gtgtgccatg
     4261 ccataaagtt acagtgtccc ataggtgtct catctcatgg tttcagtaaa acataatgag
     4321 gtctgagaac ggtaatgttt gcgccggttt ttgtggtatc cggcgggaat agcgcgtggt
     4381 gtgagagact gtttttttga tcgttttcac aaaaatggaa gtccacagtc ttgacaggga
     4441 aaatgcagcg gcgtagcttt tatactgtac ataaaaccag tggttatatg tacagtattt
     4501 gtttttaatg gtttgtttta aaagtcaaag aggggtttat tatgggtagt aatggcgcag
     4561 ataacgcaca taacaatgct tttggtggag ggaaaaatcc gggcattggt aataccagtg
     4621 gcgctggaag taatggtagc gcatcaagta accgaggaaa ttccaatgga tggtcatgga
     4681 gtaataagcc tcataaaaat gatggcttcc acagtgatgg ttcttaccat attacatttc
     4741 atggggacaa taattcaaag cctaaacctg gtgggaatag tggaaataga ggtaataatg
     4801 gtgatggatc gagtgctaag gctggagaga taacaattac acctgacaac tcgaaacctg
     4861 gtcgttatat ttcgtcaaat ccagaatatt cattgttagc aaaattaatt gatgcggaat
     4921 caattaaagg tacagaggta tatacttttc acaccagaaa aggtcagtat gtcaaggtta
     4981 ctgttccaga tagtaatatt gataaaatga gagttgatta tgtgaactgg aagggaccga
     5041 aatataacaa taaacttgtg aagaggtttg tgagccagtt tttattattt aggaaggaag
     5101 aaaaagaaaa aaatgaaaaa gaagccttgc taaaggctag tgaacttgtg tctggtatgg
     5161 gggataaact tggcgagtat cttggagaaa aatataaaaa tgtagctaag gaagttgccg
     5221 gtgatattaa aaactttcat ggccgtaata ttcgtagcta taatgaagca atggcttcac
     5281 tgaataaagt gttagcaaat ccaaagatga aagtaaacaa atctgataag gatgccattg
     5341 tgaatgcctg gaaacaggtt aatgctcagg acatggctaa taagattggt aatcttggca
     5401 aggcatttaa ggttgctgat ttagctataa aggttgagaa aattagggaa aaaagcattg
     5461 agggatacaa tactggcaac tggggacctc tcctgctgga ggttgaatca tggatcatcg
     5521 ggggcgttgt tgctggagtt gctattagtt tgtttggggc tgtgttgagt tttctcccaa
     5581 tctcaggact tgctgttact gtgttggggg taataggaat aatgacgatt agttacttgt
     5641 catctttcat tgatgcaaat cgagtttcga atataaataa cattatatct agtgttattc
     5701 gatgacatga gtgtcagggg agtatacctc ccctggtaaa tagtttatat gttcctataa
     5761 agaaccaaag gtataaatat gtgaatatat atatcccaat gtatagtgtt atatagaaga
     5821 aagacaaaaa caggtcattt tttgacatta atacaaatgt ttttgatgac aaggttagtt
     5881 ctgtgtcagt tagcaagaaa cagtatatca tggatatata aagaatagtg aaaggagtta
     5941 atatctttag cgcccgcaga gatgatgact ttggttttaa ctctaattct ttgtgtgtga
     6001 atatgaataa gctgattgca gctaaagggg ctgtatgaac atagagattg aatgcttcgc
     6061 ttaggggcgg gttgtttgat gaatgaaatc ctggtaggga taggtatgcg atgatttttt
     6121 caaggagtgg tattggtgca ttattataac tgaaaaatat taatgcgaat ggggaaagta
     6181 agagcagaag actgaatgat attttttttg atatcttatt tctgtctttt atatccatgt
     6241 tgctttcttt ggatggttgg aaattatgtg tgggaaaatt ctattgatct tgttttttat
     6301 catgacactt agtgcatgtc aggtcaatca catccgtgat gtgaaaggag ggactgttgc
     6361 tccatcatca tcatccagac taacaggagt tgaaattaag taaaagatca aaggatcctc
     6421 ttgagatcct ttttttctgc gcgtaatctg ctgcctgtaa acgaaaaaac cgcctgggga
     6481 ggcggtttag tcgaaggtta agtcagttgg ggactgctta accgaggtaa ctggcttcag
     6541 cagagcgcag ataccaaata ctgtcctttc agtgtagccg cagttgggcc atcacttcaa
     6601 gaactctgta agcatctgga taaatctcgc tctgctaatc cggttaccag tggctgctgc
     6661 cagtggcgtt aaggcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6721 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6781 caccgggccg agataccaac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6841 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6901 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6961 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcggcgc
     7021 ggccttttgt tgccctttgc ctttttactc acatgttctt tccggcctta tcccctgatt
     7081 ctgtggataa ccgtatttcc gcctttgagt gagccggcac cgcttgccgc agccgaacga
     7141 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg tcggaagctg cattttctcc
     7201 ttacgcatct gtgcggcatt tcacacccgg cat
//
