LOCUS       NZ_AP022119             4187 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella quasipneumoniae strain WP5-W18-ESBL-06 plasmid
            pWP5-W18-ESBL-06_7, complete sequence.
ACCESSION   NZ_AP022119
VERSION     NZ_AP022119.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194518
            Sequence Read Archive: DRR199354
            Assembly: GCF_014169255.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Klebsiella quasipneumoniae str.
            WP5-W18-ESBL-06 isolated from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 184x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 09:05:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,941
            CDSs (total)                      :: 5,816
            Genes (coding)                    :: 5,600
            CDSs (with protein)               :: 5,600
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 216
            CDSs (without protein)            :: 216
            Pseudo Genes (ambiguous residues) :: 0 of 216
            Pseudo Genes (frameshifted)       :: 89 of 216
            Pseudo Genes (incomplete)         :: 132 of 216
            Pseudo Genes (internal stop)      :: 31 of 216
            Pseudo Genes (multiple problems)  :: 33 of 216
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022119.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WP5-W18-ESBL-06"
                     /isolation_source="wastewater"
                     /db_xref="taxon:1463165"
                     /plasmid="pWP5-W18-ESBL-06_7"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6538 N 139.5126 E"
                     /collection_date="2018-02-13"
     gene            101..292
                     /locus_tag="H7R83_RS29520"
                     /old_locus_tag="WP5W18E06_P70010"
     CDS             101..292
                     /locus_tag="H7R83_RS29520"
                     /old_locus_tag="WP5W18E06_P70010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            292..714
                     /locus_tag="H7R83_RS29525"
                     /old_locus_tag="WP5W18E06_P70020"
     CDS             292..714
                     /locus_tag="H7R83_RS29525"
                     /old_locus_tag="WP5W18E06_P70020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_025714834.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTSINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKREPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1668..2300)
                     /locus_tag="H7R83_RS29530"
                     /old_locus_tag="WP5W18E06_P70030"
     CDS             complement(1668..2300)
                     /locus_tag="H7R83_RS29530"
                     /old_locus_tag="WP5W18E06_P70030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            complement(2300..3415)
                     /locus_tag="H7R83_RS29535"
                     /old_locus_tag="WP5W18E06_P70040"
     CDS             complement(2300..3415)
                     /locus_tag="H7R83_RS29535"
                     /old_locus_tag="WP5W18E06_P70040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            complement(3760..3873)
                     /locus_tag="H7R83_RS29545"
                     /pseudo=""
     CDS             complement(3760..3873)
                     /locus_tag="H7R83_RS29545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     Typing          complement(3761..4167)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.154"
                     /coverage="99.0"
                     /evalue="9.91e-169"
ORIGIN
        1 cgctcgccgc agccgaacgg caggagcgta gcgactgagt gagcgaggaa gcggaaaaga
       61 ggagatccct acactgacca ctgacgctct gaggcctctt atgacgaaac aacagaaaac
      121 agcgctgaac atggctaaat tcatccagaa tcagtctctg ctcctgctgg agaagcttaa
      181 cgagcttgat ctcgatgttg aggccgacct ttgtgagaaa ctccacgatg atgcggagca
      241 tcttttccgt acactgtcct cgagactgga tgaattacag gatggtaatt gatggccaga
      301 gtgtctatta gtgaagcggc acggcttgtt aaggttagcc gtcccacaat ttacaagatg
      361 attaacagtg ggaagttaag ttacacctct gttgtaaagc acgggaaggc tattaaagtt
      421 attgatactt cagagcttat tagagtgttt ggttctcttg atggtgtcat cgatactgta
      481 aaatatgatg taaaaagtga cgcagaatct acaagtatta acagcgttgg tttacatgat
      541 ttacaacata gaatcgctct acttgaagct gaaaacgatg gtcttaaagg ggctgttaag
      601 gctcgagatg agcatattga ttcccttcgt caggctatgc agcttctgga gcacaagcgc
      661 gagccttctt caccacccca ttccccgtgg tggaaattct ggaaaaaatc ctgatcctgc
      721 catgcttgag ttttcctctc cggccatccc agggagttcc tgaaccttcc caaaaatcgc
      781 cagatctctg aacgctcctc acgactaaaa atacccacac actacgcaaa attaaaattt
      841 tgcagattcc ttatttaaaa caattggtta atgaaaaatt aatgtgcagt gatgtgttac
      901 aaagtgcaga tccaagtcgt ggacgactct atggcgaatc gtttacgaat tgcccacgat
      961 tcatatgtga atcacgcttt attatcatgt tgttatgatg aatcgactct tgattcaatt
     1021 catcaatttg ggcgtttttt gttcatggtg actcttgatt caattcacga ttcgaatcgc
     1081 cggtgaatcg tgtggtgagt cgtccggcga cttgccataa cgtgattgcc ggccgtcgac
     1141 gaacccgatt cctgaacgtg ctgaaaaccg gttttccggc tgcgccggca acgcctcacg
     1201 gccacgcaat aaattgcgtc cccgttcacc gttttcggat cagaagaacg ccggaaacag
     1261 gacttaccct ggctgaatgg cgtgaatttc cgcgctatgc acttgcgcgc atactcatgc
     1321 atgccgtaaa aacagagcct gcgcgtttct ggcgggtttt cgggtggttt gttgcctgtt
     1381 ttaccggttt cccgtcagaa acgtcctgag gccgttttag cggtgcgtac aattatgatt
     1441 ttatgttaaa ttgttttaag tgaatagaga gaatataact taagggagca tcttgtgctg
     1501 ggtcttttac attagatctg cacaatcatt gttttggcat ctggtgatgt ctttaggacg
     1561 tttgagattg ctgataacta aagtgtatga tgctttctat acgttatccg gcgtttaata
     1621 acaccctggt gataagccag cccgtgaagg gctggcttga tttggattta tatttttgaa
     1681 ataaaatctt ttatttcatc aaagcaattg tctgctgtgt ttattattcc atttttcatg
     1741 gatattccta ttcttgagtc ggattctcta ttgcagctaa gatagacttg taccttagct
     1801 tttttttcgt ttgttggata agtgtactct ttgtcactcg ctgagatgtc atctttatta
     1861 tttttaattt tttcgaaaaa ttctattatg tattcatata acgctcgagt gcttgcgtgt
     1921 tcaattatca gatcttcaat ggcaccttgc ttgtcttttc ctggcattat aaagaaagaa
     1981 atttttgtat tgccattcat agctatctcg ttatgcgagc tgggggagga gatttgtagt
     2041 tctcgcaatg tggcaattat tgatctttct gtgtcagaaa atgatgtgtc agcatctaat
     2101 ataataaaaa tactttcttt ttcatcgaaa gaatcaatgt ttttgaatgc tctaagtgct
     2161 tgttttaatt ttgattttcc atttattttt tcgacaaaaa catcggtaat gccttgtatt
     2221 tcaagtaggt tgcatagaaa atgaaaatca tcatcaccct caacgaaaat gatctttttg
     2281 ctattattta tgtctttcat tatcttacct cccagtcgtt ctcaactgag taggagaaat
     2341 catccatatt gaattgtacg gctgttggtt ttagggagtt cttttctctt cctagtctaa
     2401 tataagcgat atcttcattt ttgctatcta tttcgtttat tgctcgtatc acatcttgac
     2461 tatgagttgt tgcaaatatt tgattgttgt tttgttttgc agatgaaatt aatgatttta
     2521 ttatgtcttt aattactgag taatgtattc cgttctcgat ttcatcaata agaataattg
     2581 agttttcctg taccagcact gagctgataa aagataatgc tcgactgata ccctcgccaa
     2641 gcattgatat ttcatttagc tcactaaatc caatgtctaa atatatttcc ttattgttac
     2701 ctatcgcaga tattgctata tctacaattc tgtcatctat tttttttaag ttttctagta
     2761 tgtctttttt ccttttttgt gttagtaggc tagatacatt tgtaattgtt gcctctttgt
     2821 ttatctttct ggatgttgtt attattgttg ctgtcttaaa tttataatta atcccatccc
     2881 tttcgttttt tatttcggat tttatttcta cgccatcttc tttcaattgc atagagaaaa
     2941 gcttattgga aaccctgttt ttcgcattgg gtgtctcggt cacatttatt tttaaagagt
     3001 ttactgtggc ctgagaaccg atagtttgac gaggtatgtt ttggttagat agtacggtgt
     3061 caacgtttag agacaaactt gattctaatc tctgatttgt ttctgtttca tatgtttgtg
     3121 ttacttcaga gcgttcatct cgaatgacta tggacatctt ttgtgatgtg tccatatcat
     3181 gaaagtaact aacccaaaat ggcttgtttt tgtttatttc tgccatttct tttctgaatg
     3241 cttgaatgtt taacaaaaca gccgggtttt ttacatcata aaaacaaaaa atagcatcca
     3301 gtatagatgt tttcccgtag ttgtttttcc cagctatgat atttaaagtc tttaattgct
     3361 ttagatatag ctcattgaag catttgaagt tctttaaact tatttccttt atcatgatag
     3421 cctctgttcg tgtttttgtt atatgccata cgatttgtta gtaccttaat aaaatgtaca
     3481 gctttcattg tgattagcta gactttatgt caaagattaa ctttgattgc ttgtgtttta
     3541 atcgattttt tttactttct tcttgagatt atctggcagc agccgtaagc ttccggctgt
     3601 gaacgaaaaa aaccgccttg gcgggcggtt ttttcgaaag gttcagtaga gttggcgctc
     3661 tatgaaccga ggtaactggc ttaaagggag cgcagtcacc aaaactgttc gtttagttta
     3721 gctttaactg gcgtataact tcaagactaa ctccctaaat cagttaccag tggctgctgc
     3781 cagtggcgct ttgtcgtgtc tttccgggtt ggactcaaga cgatagttac cggattaggc
     3841 gcagcagtcg gactgaacgg ggggttcgtg catacagtcc agcttggagc gaactgccta
     3901 cccggaactg agtgtcaggc gtggaatgag acaaacgcgg ccataacagc ggaatgacac
     3961 cggtaaaccg aaaggcagga acaggagagc gcacgaggga gccatcaggg ggaaacgcct
     4021 ggtatcttta agtcctgtcg ggtttcgcca ccactgattt gagcgtcaga ttccgtgatg
     4081 ctcgtcaggg gggcggagcc tatggaaaaa cggccgctgt gcggcctttc cttctttcct
     4141 ccatgcgatt actcggtttt ctcgagcgtt tctacgcatg ctcctgc
//
