LOCUS       NZ_AP022118             5714 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella quasipneumoniae strain WP5-W18-ESBL-06 plasmid
            pWP5-W18-ESBL-06_6, complete sequence.
ACCESSION   NZ_AP022118
VERSION     NZ_AP022118.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194518
            Sequence Read Archive: DRR199354
            Assembly: GCF_014169255.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Klebsiella quasipneumoniae str.
            WP5-W18-ESBL-06 isolated from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5714)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 184x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 09:05:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,941
            CDSs (total)                      :: 5,816
            Genes (coding)                    :: 5,600
            CDSs (with protein)               :: 5,600
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 216
            CDSs (without protein)            :: 216
            Pseudo Genes (ambiguous residues) :: 0 of 216
            Pseudo Genes (frameshifted)       :: 89 of 216
            Pseudo Genes (incomplete)         :: 132 of 216
            Pseudo Genes (internal stop)      :: 31 of 216
            Pseudo Genes (multiple problems)  :: 33 of 216
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022118.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5714
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WP5-W18-ESBL-06"
                     /isolation_source="wastewater"
                     /db_xref="taxon:1463165"
                     /plasmid="pWP5-W18-ESBL-06_6"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6538 N 139.5126 E"
                     /collection_date="2018-02-13"
     gene            complement(76..225)
                     /locus_tag="H7R83_RS29485"
     CDS             complement(76..225)
                     /locus_tag="H7R83_RS29485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_029497523.1"
                     /translation="MKKVKAIFLFILIVSGFLIVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(310..567)
                     /locus_tag="H7R83_RS29490"
                     /old_locus_tag="WP5W18E06_P60010"
     CDS             complement(310..567)
                     /locus_tag="H7R83_RS29490"
                     /old_locus_tag="WP5W18E06_P60010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_022644819.1"
                     /translation="MGLKLDLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGCFDVKNEWVSLLQPYFKHKINLSDSSYFVSFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(577..2262)
                     /locus_tag="H7R83_RS29495"
                     /old_locus_tag="WP5W18E06_P60020"
     CDS             complement(577..2262)
                     /locus_tag="H7R83_RS29495"
                     /old_locus_tag="WP5W18E06_P60020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_023284666.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPNMMSK
                     IMTSLPADTVTETPVSTLPLEQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNGRITSTEKTIPGARTAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYHPAPKTEDIKGLGDLKKGTPKTPMQGGGGRRKRWVGDKGRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            2589..2834
                     /locus_tag="H7R83_RS29500"
                     /old_locus_tag="WP5W18E06_P60030"
     CDS             2589..2834
                     /locus_tag="H7R83_RS29500"
                     /old_locus_tag="WP5W18E06_P60030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023284667.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKSLPKAVKQQVRERV
                     LRAIQDERESRLAKKMRREKYEAAEDTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            complement(3574..3975)
                     /locus_tag="H7R83_RS29505"
                     /old_locus_tag="WP5W18E06_P60040"
     CDS             complement(3574..3975)
                     /locus_tag="H7R83_RS29505"
                     /old_locus_tag="WP5W18E06_P60040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_022644821.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHLSGQVSEQRDTASADQNEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3975..4166)
                     /locus_tag="H7R83_RS29510"
                     /old_locus_tag="WP5W18E06_P60050"
     CDS             complement(3975..4166)
                     /locus_tag="H7R83_RS29510"
                     /old_locus_tag="WP5W18E06_P60050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_022644820.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLNASQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4646..4795)
                     /locus_tag="H7R83_RS29515"
     CDS             complement(4646..4795)
                     /locus_tag="H7R83_RS29515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162833787.1"
                     /translation="MPSEHDEQRDATDTQLPGEYDLFDLMAQVTEENLHAPVDSGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            5451..5564
                     /locus_tag="H7R83_RS29940"
     CDS             5451..5564
                     /locus_tag="H7R83_RS29940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_223178531.1"
                     /translation="MQEPPFQPYCCACSGNCSLGSNPERHDKSPLAAATGN"
                     /name="replication initiation protein"
     BGC             1..5714
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(4789..5004)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="83.258"
                     /coverage="100.0"
                     /evalue="7.539999999999999e-48"
     Typing          complement(5368..5553)
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.617"
                     /coverage="99.0"
                     /evalue="6.160000000000001e-73"
ORIGIN
        1 cgggtggttt tttcgtttac aggcagcaga ttacgcgcag aaaaaaagga tctcaagaag
       61 atcctttgat cttttctact gaaccgcaat tcccgtcagc tcagaggagg atgatggtgc
      121 caccgtcccg ccctgaacat ccctgatata gtttgcctga catgcgacaa taagaaagcc
      181 agaaactatc aatataaaaa gaaaaatagc ctttaccttt ttcatatact ttccctgtat
      241 ttaaagactc cagcattcat ccctgaatac cggaagtctg tatttcaact catccttgat
      301 aaatttttat taccagttgc catcccgata atcaaatgaa acaaaatatg aactatcaga
      361 aagattgatt ttatgtttaa agtagggttg caataatgaa acccactcat ttttcacatc
      421 aaaacaacca ttgttaatat tatcctttaa aggcatcccc agactttcaa tgacagaacc
      481 atcatcacca aagtctttag aatactcttc ccctttaaac tcttcagttt tcttatcaaa
      541 ccaggttaaa tctaatttaa gtcccatact tacctctcaa agatattttt taatgtttct
      601 ttttggatcc ggccctttaa cctgcttacc cgtttttgga tcgaatgcgc cgaggtgttc
      661 gccatcactg gcgcggtacc cttcaagctc accgtgctgg gaatcccatt cataaatctt
      721 acgaccttta tcaccaaccc agcgtttacg cctacctcca cctccctgca ttggtgtttt
      781 aggtgtacct tttttgaggt cacctagccc ctttatgtct tcagttttcg gggctggatg
      841 ataatcatgg ccgtaatctt tagttccctt gcgaggtttt ttcttttcct catttaattt
      901 attttctgca tccttcgctt ttttctcctt ttgtttccgg ctctccatcg ccccggaaag
      961 cgcagcctta tctctggata aggattcatt cattgcgttt aaagatgcct gagctgcttt
     1021 taatttctca ttctgattct gaatatcctt atcaagatag cgaacctggt cctgccaccc
     1081 ggagccatat tcatgaggcg ggttgtaagt cacaaaatca tctttatttg cctcggcttc
     1141 tttcaccttt ttatcggctt cctgaacagc agttcttgca ccaggaatgg ttttttctgt
     1201 cgatgtaatc ctaccgttta aggtcgcaat atttttatct tcggcatcca gctcagcttt
     1261 cgctttatca tactctcttt tcagcccctc ttccgggtga gcggcgtccc atgcctgctg
     1321 acgacgcttt tcttcctcct gacgctgctt tacctgtgcc ggggtaagaa catctgtcac
     1381 cgacacataa accggcttct gtccggtctc tttcgggaaa cgaataacgg cctcacggga
     1441 gttaccaccg gccgtaaaac cagccggacg tgaatcacct ttttcagcaa caatgccttt
     1501 tggcggggct ttcgcggccg gaacaccttt cggtacgctc acctgcagag acgggagacc
     1561 cggaatcgac acactgaata cccccggacg ttttgtcggt ttcgcatcca ccacaggaac
     1621 actcattggc cgacccgcga caaccgcaat atgctgccgc tcatccttca ccacatccac
     1681 aacccgttgt ctgacacgaa ccgtcgcctg ctccagtggt aaagtactta ccggagtctc
     1741 cgtcaccgta tcggccggta atgacgtcat aatttttgac atcatgttcg gatcatcttt
     1801 tgcaatctca gaaggcagca caccgtagat cgctatcccc cacagaccaa acttaaacgg
     1861 acctttcagg gcggccagca catcagcaac agcggccgac aacgcatcgc cagagacgga
     1921 taaagccagt ccctcagccc ccggagtggc aagggctggc agaccgaagg ccatcgcggt
     1981 ggctgaagac tgtccgccac cagagctccc gctatgattg ccgctgttac cgccattacc
     2041 accgggttta ctcccgccgt taccaaactc acccgggtta tagttatgaa tatccccgtt
     2101 aggtgtatgc gtcgtacccc agcccggccc gctgtttgga tccgtaccac caccagatga
     2161 agggccacct ttacctgacg taccgttcac atttccactg gcctgaccac cattgtgtcc
     2221 cagacctgaa ttacccggac ctcgtccgtc tccgccactc atatcgttat cctctttgac
     2281 atttaaaaca ataaattaaa accaatatac tgtatatatg aacagtataa ttattacctt
     2341 acccttgctg ttttcaggtg tcaatatatg agattagttc tcatcttcag gcacaaaaaa
     2401 gcggccagag ccgcctgtca gcaggttgcg tcttttcccg aaagtttccc ccggcaacaa
     2461 atttcattat ttgcgatatt catcacactt gcttttatgt aagtacatgc atatctgaaa
     2521 gcggactgga atgaggagag gactaccaat taagctgttc aggaacccag agacacccgg
     2581 aggccaccat gacacacctg aaactcaccc ccgctgaaga gaagcgtctg gcggacgcct
     2641 ttgtcgccca ggcgctggcc atgggcctga aagggaataa atcactgcca aaggcggtaa
     2701 aacagcaggt gcgggaaagg gtgcttcgcg ccattcagga cgaacgtgaa tccaggctgg
     2761 cgaagaaaat gcgccgggag aagtatgagg cggcggaaga caccttcacc tggcagccgc
     2821 agcgccggcg ttgacgattt accataacat ccgttgcccg caccgcctca ggcaccctca
     2881 gcgcgtttct gacggaaaac cggcaaaaca ggcaacaaac cacccgaaaa cccgccagaa
     2941 acgcgcaggc tctgttttta cggcatgcat gagtatgcgc gcaagtgcat agcgcggaaa
     3001 ttcacgccat tcagtcgggg taagtcctgt ttccgatgtt cttcttaccc gaaaacggtg
     3061 aacggggacg caatttattg cgtggccgtg aggcgttgcc ggcgcagccg gaaaacccgt
     3121 tttcagcacg ttcaggaatc cggttcagcg acagccggca atgacgtgat ggcaagtcac
     3181 cggacgactt agcacacgat tcaccggcga ttcgactcgt gacttgactc tagagtcacc
     3241 aaggtcaaaa aaacaccaaa tgaacgactc aactctagag tcaagtcgtc ataagttact
     3301 gatagtatgg agtgagtcgt cgatgactct atgacgagtc gtcggcaagt cgtacgcgaa
     3361 tcgccgacga cttggattta ccttacagaa ggtaatacag cgtaatttaa taaaacctaa
     3421 ccaactgata ataaggagtt atctgcaaaa ttttaatttt gcgtggtgtg tgggtatttt
     3481 tcgtggtgag gagtgttccc ggatctggcg atatctggga aggttcagga actccccgga
     3541 atggccggag aggaaaactc aaccatggca ggatcaggat ttcttccaga atttccacca
     3601 tggcgccggg gattcagtta attttgcctg gtgattttca agaaggagca tggcctgttt
     3661 caggctgtca atgtgcgaat ccttggacaa aagtaaggtg ttcaggtgtt caatctcctg
     3721 tttcagatga cgaatcacct gttcattctg gtcagcagag gctgtatcac gctgttcaga
     3781 gacctgtcca gaaaggtgtt cacccttagg ttgctcaaag ggaccgaaga cacgcaaaag
     3841 ctctgaagta tctaccattt ttgcattacg ttcaccgcta caggtggaca agtcacctgc
     3901 tttaattaac ctgtgcagcg ttgttctact cttacccgtc agtctggcag cctcactaat
     3961 agatactctg gccatcagtt accatcctgt gatgcattca gtctcgagga cagtgtacgg
     4021 aaaagatgtt ccgcatcatc gtggagtttc tcacaaaggt ccgcctcaac atcgagatca
     4081 agctcgttaa gcttctccag caggagcaga gactgattct ggatgaattt agccatgttc
     4141 agcgctgttt tctgttgttt cgtcataagg gccctcagag catcagtgct cagtgtaaac
     4201 cggtaagggg gtcggatgta cgaatggttc agagaacgga tctgaggtaa tcctgtagtt
     4261 attttgtagt ttatctgtag tcatcgcact aaaaatcagt attaaatcat caacttttgt
     4321 agtcttcctg tagtttacct gtagtctgga aaaatcatcc gttcatgcaa gcatgaacga
     4381 agcagttaaa gacgcttttt cgtcgccgat gctccttaac ctgcgcagat cacgcggcca
     4441 gaatgataat tttttacccc ttaaatggtc atacacctaa cgcagccgcg attttcatcg
     4501 caactgcatg aattaaaagg taaaaactga aatctccgtt tatttatgta atacgcaacg
     4561 tattacaatc acctgtaatt acatgaaaat aaagaaataa aatgtaatac cggcgtatta
     4621 ccacgtacgc gggcaagcga aaaagctata acgcttcccg gccgacaggc ggcccagaat
     4681 ccacaggggc atgcaggttc tcttcagtaa cctgcgccat taaatcaaaa aggtcatatt
     4741 cgccgggaag ctgcgtatca gtagcatccc gttgttcatc atgttcagat ggcatggggt
     4801 gatatccagt agctgagagg ttgttagcgg gtttcggggc gcagccctga accagtcacg
     4861 tagcgctagc ggagtgtgta ctggcttcct atgttggcat gagcgtggtt accagggaag
     4921 tgcttcatgt ggcaggagaa aaatgtggcc ggaacgcgta agtgctgaat gccggttccg
     4981 gggaaatgac gcctcctcgc tcactcggtc gctcacgctc cggccgttcg gctgcggcgg
     5041 gcgttgccta cgctcacaca acagactcat accttatcca cagaatcagg ggataacgca
     5101 tcacagaaca tgtgcgcaaa aaaagaaagg acgaaaaggc cgcgccgcag gcgctttttc
     5161 cataggctcc gcccccctga cggagttcac ataacagaca ctttttttgt gcatctgtgg
     5221 gagccgtaag gctcaaccat gaatccgcca gcggtgacga aacccgacgt ggcttaaaga
     5281 tcatcaccgt ttccggtgaa tcgctccctc ctgcgccccc acgttcccgc catatcggtt
     5341 accggatacc tttccgcctt tctcccttac gggacacgtg gcgctttctc atagctcacg
     5401 tcttctggta tctcggacca acttaggcag ttcgctccgg gccgggctgt atgcaggaac
     5461 ccccctttca gccctactgc tgcgcctgtt ccggtaactg ttctcttggg tccaacccgg
     5521 aaagacacga taaatcgcca ctggcagcag ccactggtaa ctgaaatgtg cgagatatgt
     5581 ttcgaagagt tcttgaagtg gtggcctgac tgcggctaca ctggaaggac agatttggtg
     5641 acatgtctcg cacaagactg ttaccacggt tcagaaactt gccggtttgc tgaaccttcg
     5701 aaaaaccacc ttgc
//
