LOCUS       NZ_AP022117            12566 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella quasipneumoniae strain WP5-W18-ESBL-06 plasmid
            pWP5-W18-ESBL-06_5, complete sequence.
ACCESSION   NZ_AP022117
VERSION     NZ_AP022117.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194518
            Sequence Read Archive: DRR199354
            Assembly: GCF_014169255.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Klebsiella quasipneumoniae str.
            WP5-W18-ESBL-06 isolated from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12566)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 184x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 09:05:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,941
            CDSs (total)                      :: 5,816
            Genes (coding)                    :: 5,600
            CDSs (with protein)               :: 5,600
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 216
            CDSs (without protein)            :: 216
            Pseudo Genes (ambiguous residues) :: 0 of 216
            Pseudo Genes (frameshifted)       :: 89 of 216
            Pseudo Genes (incomplete)         :: 132 of 216
            Pseudo Genes (internal stop)      :: 31 of 216
            Pseudo Genes (multiple problems)  :: 33 of 216
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022117.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12566
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WP5-W18-ESBL-06"
                     /isolation_source="wastewater"
                     /db_xref="taxon:1463165"
                     /plasmid="pWP5-W18-ESBL-06_5"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6538 N 139.5126 E"
                     /collection_date="2018-02-13"
     gene            complement(101..343)
                     /locus_tag="H7R83_RS29405"
                     /old_locus_tag="WP5W18E06_P50010"
     CDS             complement(101..343)
                     /locus_tag="H7R83_RS29405"
                     /old_locus_tag="WP5W18E06_P50010"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039022311.1"
                     /translation="MAFENIERRFQRWKVIMVPGAKSYESWDEMVTGENFPEEARYKYK
                     KHRTKAIDLTLKNPDYVHTMSENGITVRYRLERWR"
                     /name="hypothetical protein"
     gene            complement(359..709)
                     /locus_tag="H7R83_RS29410"
                     /old_locus_tag="WP5W18E06_P50020"
     CDS             complement(359..709)
                     /locus_tag="H7R83_RS29410"
                     /old_locus_tag="WP5W18E06_P50020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039022312.1"
                     /translation="MRTKAIIAAVLLATAGTAAAAPVATTYICNDGTFIHLNEQNGRLT
                     ASWRGADIPVTKIETGKLTASLNGEQGSSTLQLVVGYNTTPNPIAIITTVVNGSMNSQS
                     CKPDTASKHIDL"
                     /name="hypothetical protein"
     gene            complement(810..1001)
                     /locus_tag="H7R83_RS29415"
                     /old_locus_tag="WP5W18E06_P50030"
     CDS             complement(810..1001)
                     /locus_tag="H7R83_RS29415"
                     /old_locus_tag="WP5W18E06_P50030"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039022313.1"
                     /translation="MIPGRTQILKATADIAKALGTDTADVERHFLQHAVVLARLRQEGK
                     RPRIKAWMAENIGTPSAM"
                     /name="hypothetical protein"
     gene            complement(1004..1195)
                     /locus_tag="H7R83_RS29420"
                     /old_locus_tag="WP5W18E06_P50040"
     CDS             complement(1004..1195)
                     /locus_tag="H7R83_RS29420"
                     /old_locus_tag="WP5W18E06_P50040"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039022314.1"
                     /translation="MQSIQQTTPARYPGLSIENGEIRQHGKQRHCAANRKGQYQTTLRD
                     SAGRYQTVSILSLYAWEA"
                     /name="hypothetical protein"
     gene            complement(1307..1948)
                     /locus_tag="H7R83_RS29425"
                     /old_locus_tag="WP5W18E06_P50050"
     CDS             complement(1307..1948)
                     /locus_tag="H7R83_RS29425"
                     /old_locus_tag="WP5W18E06_P50050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324021.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_039022315.1"
                     /translation="MITNTTAVAYMRASTQEQDANRAREEIALFAKEHGITIRGFYAEN
                     VSGTYLERPELNRLLEEAKPHDILLLEKLDRLSRLSAEEWSLLKERIRTKRLRIVCIDI
                     PTTLAVLQGGQPVGAGGFDVRRVVLDALNDMLLDIAAAWARDDYETRRKRQQQGIEKAK
                     KLGRFKGKQKDEELRSKIRTLLDTSSMSQNQIAQLIGCSRNTVSRVARGE"
                     /name="recombinase family protein"
     gene            2213..2899
                     /locus_tag="H7R83_RS29430"
                     /old_locus_tag="WP5W18E06_P50060"
     CDS             2213..2899
                     /locus_tag="H7R83_RS29430"
                     /old_locus_tag="WP5W18E06_P50060"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039022316.1"
                     /translation="MENEQIAPVDFEGANRIYLADVLAEACSALPELPDLPVPSLDGDD
                     AAVASLLHLMRQRDEMASAIGVLNFELGKLKEAAEAAHDRLVDAEARIAAARDALLAGN
                     EPEPRDAMLALVVDQIRADVQAADAAVDIKSREVGALDAQMNTAVHQIEGAIDELICSC
                     RIARARALELALLNELRALAVHDGDKNRFNRAFVPAPELALLSSNYGQIEMLRPGRMPH
                     LNRGVL"
                     /name="hypothetical protein"
     gene            2899..3297
                     /locus_tag="H7R83_RS29435"
                     /old_locus_tag="WP5W18E06_P50070"
     CDS             2899..3297
                     /locus_tag="H7R83_RS29435"
                     /old_locus_tag="WP5W18E06_P50070"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_052249905.1"
                     /translation="MSMFDQLVARLRDEQALTKSLNPGDALAAVGNGDANAPVLDQRAL
                     TIGVAPGVVACHNARLNQIADAQLLQEVRASGVVSAADAVALDNALAASRHLPPEARAA
                     GMESVRQQLNAAAAAPLSPAARGINWDE"
                     /name="hypothetical protein"
     gene            3285..3653
                     /locus_tag="H7R83_RS29440"
                     /old_locus_tag="WP5W18E06_P50080"
     CDS             3285..3653
                     /locus_tag="H7R83_RS29440"
                     /old_locus_tag="WP5W18E06_P50080"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039022317.1"
                     /translation="MGRVKGLPRHLQGKTRLPLLGGCFSKGHRLALLAVMPIIEARPGE
                     RFDGEAAKLALFQDLLLKAGQPPAFALHEPSLYRFGAALRNNRRGLTDTGIEKANELLD
                     QHLFSRALDAIVATKQTD"
                     /name="hypothetical protein"
     gene            3664..3960
                     /locus_tag="H7R83_RS29445"
                     /old_locus_tag="WP5W18E06_P50090"
     CDS             3664..3960
                     /locus_tag="H7R83_RS29445"
                     /old_locus_tag="WP5W18E06_P50090"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039022318.1"
                     /translation="MSLYPKDKNPVFQPGGYGLRYDGQAEAIARAGEPPINYAPGQELS
                     RAAPALPEHPTHGAARAFRDHLTGEYVVAAGPVEVRTRVSAEEAALLWATLTA"
                     /name="hypothetical protein"
     gene            3939..4493
                     /locus_tag="H7R83_RS29450"
                     /old_locus_tag="WP5W18E06_P50100"
     CDS             3939..4493
                     /locus_tag="H7R83_RS29450"
                     /old_locus_tag="WP5W18E06_P50100"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039022319.1"
                     /translation="MGYFDCVKLTDAEWPDHMVEGRDAALRQAMRGGVSQWCRDKVVQL
                     LPVLRCPRGEWRDAELKRVDELADALGLDDMGLFAYGAMIVKRRAYVAGSLSRAKPPEP
                     LAVVLDDGEHRCTVYAMPHPTLGVPEISERGEDGYWTISAGGCAWIKARVSLGEGVRLW
                     NELVNCARYPDAPVLTRGMLE"
                     /name="hypothetical protein"
     gene            complement(4717..7476)
                     /locus_tag="H7R83_RS29455"
                     /old_locus_tag="WP5W18E06_P50110"
     CDS             complement(4717..7476)
                     /locus_tag="H7R83_RS29455"
                     /old_locus_tag="WP5W18E06_P50110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="WP_053266538.1"
                     /translation="MAIKIPVSAQFDAADLQQQIKMVNDQIRILASQVGNANKQKWEPI
                     NLKSKDDLQGFIKQMDLLMKKQTDFATKMKQTGQGGNPLFADFKKMAEGRLGEQIKYME
                     SVLGWAGVEFSNLPAPKPPKKPPVVPPAPPSPPEPAWRKELGRQGMSGLHSILGNAGPI
                     GGTISNALNTGMSSGASAGMAGLVGGLAALGVGKIIGAIADKIGQAQDNAIGLDKLYRQ
                     VGGMVAYSNLRSGVGDIANRLGMNNADAIGMASTYTRASNLRPGMNLGTGLLVSGGLAR
                     GYGLDPNSVAASFGSLGATGVTNSDQSIRRTGMMMGEAIAKSGAFAQAGDAMTAITDFA
                     NTQARMSLSQPNIAGYGGALAGLMSSGVPGMDLQGAGSLLNRMNGAVMHGGNMGEASQA
                     FSARLGLRRGINDPMALQALQEGGAFSTVSSMLGKGSLYNQTTGKSFSGNETMLDMIRH
                     DIHRQYGYGSNATKALANHLGIGVNEAMLVDQMSSRDINGASSRLKRLGIDEKSINMSS
                     LPVLGQIESGKGLEALGNQYLKRSDLSKEERRGLIDALKGANPEKLKDALAEVASKHGG
                     TTTEGSQIRDNIAKLNNTLEDYSKRALPALDMMRMALVNMTGGSEASVRKQYYDSNMRE
                     ASSEIGAKYKDRRAAIQSQIDALTAKGVGLQSSGPDREKLVKLKAEMQSLNDQEGADRT
                     RAAERIKLQTYGASSSTEAALSTGDGYIQSPAAAAANGGGGKYRLNSDMKKRLERLRPY
                     FEEAGRKYNIDPRRLEAIAATESGFNNNLTSSKRAQGIMQVLPTSAPGANLSDERENIM
                     AGAMVYSQMRDRYGYLGQDEILRGYNGGSRRGSAENVAYPGKVNSYYRDLAQVDDMTPG
                     SNGNKGAVGEMYGTMRVVHEDKEGKQVKPPESVSMSGTFIQPSSWGNR"
                     /name="transglycosylase SLT domain-containing protein"
     gene            7863..8042
                     /locus_tag="H7R83_RS29460"
                     /old_locus_tag="WP5W18E06_P50120"
     CDS             7863..8042
                     /locus_tag="H7R83_RS29460"
                     /old_locus_tag="WP5W18E06_P50120"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039022310.1"
                     /translation="MKFRASAICDLMGVSRRQLDRWIKAGTFPLPDGRDAKGYWWEADR
                     VATRMISERKERGM"
                     /name="hypothetical protein"
     gene            8045..9184
                     /locus_tag="H7R83_RS29465"
                     /old_locus_tag="WP5W18E06_P50130"
     CDS             8045..9184
                     /locus_tag="H7R83_RS29465"
                     /old_locus_tag="WP5W18E06_P50130"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_052249901.1"
                     /translation="MVNERRDYLFDHAVFSAALARVDPDPSVMGWLAQARTLACWCELL
                     GMDPLETCVEWSARGVNAGKGDRAMLVSALRDLDELDRLAAAGAFLRSSGVAADVAAGR
                     IAVREPSEDELAARQAHLVALRERAAAAAEKLTEARIKATGRARMLLHRATKPGSAALY
                     WQAKERAACLPLPCPDDVKCSDWRTFLPVIGQVYWNSSLLVPLYGVNESLRLEQMSVEV
                     ICGRANPEAAGSLGEKRGLKNAPREGLFYPFDLDAVGSGAPVVVCEGFMSGLALSLLIG
                     GKLPVVCAMSVGNFGATVQSLNKFVMGGHPFVLVPDIGGQDLAIDQAIPNARVIDLSAV
                     PGAEGVDGYDPFDLLARHGVEMGRKYLRGLFREARDASL"
                     /name="hypothetical protein"
     gene            9251..9532
                     /locus_tag="H7R83_RS29470"
                     /old_locus_tag="WP5W18E06_P50140"
     CDS             9251..9532
                     /locus_tag="H7R83_RS29470"
                     /old_locus_tag="WP5W18E06_P50140"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_052249902.1"
                     /translation="MTDQTTTVADLALSQSAFLVSQAIVELDAMFGEGYAAAHPAVLAQ
                     FLQTAGVIYATQLQATAAFDLAQQLDRLIGLGLGVEAGLLQAAQGASH"
                     /name="hypothetical protein"
     gene            complement(9592..10119)
                     /locus_tag="H7R83_RS29475"
                     /old_locus_tag="WP5W18E06_P50150"
     CDS             complement(9592..10119)
                     /locus_tag="H7R83_RS29475"
                     /old_locus_tag="WP5W18E06_P50150"
                     /inference="COORDINATES: protein motif:HMM:NF013561.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="WP_052249903.1"
                     /translation="MQRKSPTFSLRLPADLLEQTNELAEKTNRTRTDVITDALRAYLGI
                     PEPQGESGNRLDLMVELLQDISNTLKHNVLQKPTGRATRPRAEKLSSNSSETQGAAQRP
                     AGAEISGHYDEAEVMATIRRMREEQRDRGFRYDNKAIAQALNEAGLLQSNGRPWNNDRI
                     NTVITRRMPDLK"
                     /name="ribbon-helix-helix domain-containing protein"
     gene            10627..11922
                     /locus_tag="H7R83_RS29480"
                     /old_locus_tag="WP5W18E06_P50160"
     CDS             10627..11922
                     /locus_tag="H7R83_RS29480"
                     /old_locus_tag="WP5W18E06_P50160"
                     /inference="COORDINATES: protein motif:HMM:NF017116.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="WP_052249904.1"
                     /translation="MSQIDENSGTAADNVTPIAAAKVGRPSLQKKYPTTAFPVELEINY
                     RKGRYSSSLENVVAAMRCHSVTGIQLAFDEFFAEEGAFVNGQWYPLSDNLVTELRLRLA
                     AADFEKVTKDNARDAMFYVCDENKFDSAQRWLTAQEWDGKPRVSYFFERCFKCQGDPEY
                     LAAVSLYTWTALAGRVMNPGIKADMVPLLYGRQGARKSTGVRLMSPFPDSYSSFNLNDK
                     DDSLVRRMRGCLIGELEELRGLHGRDSESVKAFFSAAHDEWVPKFKEFKVRNPRRVVFI
                     GTTNNKQILNDSTGNRRYLPMNSGDVDCAAIEKNRSQLWAEALHLFNENGVMWEAAETL
                     ARKVHDEFRVEDPWEDEIAELLKIGVDDCEPTSFVPTKALFSQVGLMIGHTSNKQHQRM
                     AAIMSRLGYEVGRKRINGALVRGYFRRTQAVV"
                     /name="VapE domain-containing protein"
ORIGIN
        1 gattcggggt tgaggcgctt agaatggctc tggtgcgttc tggtgggagc gtgctttctg
       61 accggtaggg gcgcagcttt cgccgcgctc ctgggtaggg ttagcgccat cgttcgagcc
      121 ggtacctgac tgtgatcccg ttttccgaca tggtgtggac gtagtctggg tttttcagcg
      181 tgaggtcgat cgccttggtg cggtgcttct tgtatttgta ccgggcttcc tcggggaagt
      241 tctcaccggt gaccatctca tcccatgact cgtatgactt cgctccggga accatgatca
      301 ccttccatct ctggaaccgg cgctcgatgt tttcaaatgc catttctctc tccttgcgtt
      361 acaggtcgat atgcttgctt gcggtatctg gcttgcagct ctggctgttc atgctgccgt
      421 tgacgacggt ggtaatgata gcgatcgggt tgggtgtggt gttgtacccg acgacgagct
      481 gcagtgttga gctgccctgc tcaccgttga ggctggcggt tagcttgcct gtctcgatct
      541 tggtgacggg gatgtcggca ccgcgccagc tggcggtgag tctgccattc tgctcgttaa
      601 ggtggataaa tgtcccgtcg ttgcagatgt aggtcgtggc gactggtgcg gctgcggcgg
      661 ttcctgcggt ggcgagaagg actgcggcga tgatggcctt ggttctcatg ctgtgtcctg
      721 tgggggcgct cctcgcgccc cgggtgtatg gttttcgtta atgcgtaagt aaaaccgtat
      781 gttgttttac cttaacattt aagtttttct tacatcgctg acggggtgcc gatgttctct
      841 gccatccacg ctttgatgcg ggggcgcttt ccctcctgcc ggagccgtgc gaggacgacg
      901 gcgtgctgca ggaagtggcg ctctacatcg gcggtgtcag taccgagagc cttggctatg
      961 tcggcggtgg ctttcaggat ctgcgttctg cctgggatca tgatcatgcc tcccatgcgt
     1021 aaaggctgag gatactgacc gtctggtacc gaccggcgct gtcgcgcagg gtggtctggt
     1081 actggccttt acggttggcg gcgcagtggc gctgctttcc gtgctggcga atctctccgt
     1141 tttcaatgct caggcccggg tagcgggcag gggttgtctg ctgtatgctc tgcatgtcag
     1201 ctcctttcgt tgggtggttg atgccccgct gacctcctca ggctatcgcg gggctggggt
     1261 tgcggcggtt aggcggttgg agccaccttt gccgccgcgc tctctcttac tcgcctctgg
     1321 cgacccggct cacggtgttc cgtgaacagc cgatcagctg ggcgatctga ttctggctca
     1381 tgctcgatgt gtccagcagg gtgcggatct tgctccgcag ctcctcgtct ttctgtttcc
     1441 ctttgaaccg tccgagtttc ttcgctttct cgataccctg ttgctggcgc tttcgcctgg
     1501 tctcgtaatc gtcccgcgcc catgctgcgg cgatgtcgag cagcatgtcg ttcagggcgt
     1561 cgagtaccac gcggcgaacg tcaaacccgc ccgcgcctac tggctggcct ccctgcagga
     1621 cggcgagggt ggtcggtatg tctatgcaaa cgatgcggag gcgcttggtg cggatgcgct
     1681 ctttcaggag gctccactcc tcggcgctga ggcggctcag gcgatcgagc ttctcaagga
     1741 ggaggatgtc gtggggcttg gcctcctcca gtagccggtt gagttcgggg cgctccaggt
     1801 aggtgccgga cacgttctcc gcgtaaaagc cccggatggt gatgccgtgc tctttggcga
     1861 agagggcgat ctcctcgcgg gcgcggttcg cgtcctgttc ctgggtgctc gctcgcatgt
     1921 aggcgacggc ggtggtgttg gtgatcatgc tggttgtcct cttaaacttc cctgtcacca
     1981 tcatgcacag taagcctact catctcaact tataagatgc aaatatcgcg tcttttttgc
     2041 ggcgttttcc tgctcaggtg gcgtgtactc taaaggtgcc gctttcgacg ccccgccttt
     2101 ggggtgcggc aaggccgcgc tgcctaccgg ttctactctc ccgcgctggc tccagacctg
     2161 tcgtgacgca acgataggcg tagccgttcc ggctaaattg agagagagaa ctatggaaaa
     2221 cgaacaaatc gcccccgtcg atttcgaggg cgcaaaccgg atctaccttg cagacgtgct
     2281 cgctgaggca tgctctgccc tccctgaact gcctgatctc cctgtgccgt cgctggatgg
     2341 cgatgatgct gctgttgcgt ccctgcttca cctgatgagg cagcgtgacg agatggccag
     2401 cgcgatcggt gtgctcaact tcgagctggg taagctgaag gaagccgccg aagctgcgca
     2461 tgaccgcttg gttgatgctg aggcgcgtat cgccgccgcc cgtgatgctt tgctggctgg
     2521 caacgagccc gagccccgtg atgcgatgct ggcgctggtt gtcgaccaga tccgcgctga
     2581 cgtgcaagcc gccgatgctg cggtggatat taagagccgg gaggtggggg cgctcgatgc
     2641 gcaaatgaac accgccgtgc atcagatcga gggtgccatt gacgagctga tctgctcctg
     2701 ccgtatcgcc cgggctcgtg ctcttgagct ggctctgctt aacgagctgc gtgctctggc
     2761 tgtgcatgac ggcgacaaaa accgctttaa ccgtgcattc gttccggctc ctgagctggc
     2821 gcttctgtcg tcgaactacg gccagatcga gatgctccgt ccggggcgta tgcctcacct
     2881 gaatagaggg gtgctgtgat gtccatgttc gatcaactgg ttgctcgtct gcgcgacgaa
     2941 caggctctga ccaagtccct taacccgggc gacgcgctgg ctgcggtcgg caatggcgac
     3001 gcgaatgcac ccgtgctgga tcagcgtgcg ctgaccattg gtgtcgctcc tggtgtggtg
     3061 gcgtgccata acgcccgcct gaaccagatc gccgacgcgc agctgctgca ggaggttcgc
     3121 gcctctggtg tggtgtctgc cgccgatgcg gtggcgctcg acaatgcgct ggctgcttcg
     3181 cgtcacctgc ctcctgaggc tcgtgctgcg ggcatggaat cggtaaggca gcagctgaac
     3241 gctgccgctg ctgctcctct cagtcctgct gcccggggta tcaattggga cgagtaaaag
     3301 gcttgccgcg tcacctgcag ggcaagacgc ggctcccgtt gctgggtggt tgcttctcga
     3361 aggggcaccg gctggcgctg ctggctgtca tgccgatcat cgaggcgcga cctggtgagc
     3421 ggttcgacgg ggaggcggcg aagctggcgc tgttccagga tctgctgctt aaggcggggc
     3481 aaccgcccgc gttcgcgctg catgagccgt ccctgtatcg cttcggtgct gcgctgcgga
     3541 acaaccggcg cgggctgacg gatacgggca tcgagaaggc caatgagctg ctcgaccagc
     3601 accttttctc ccgtgccctt gatgccattg tggcgactaa acaaacagat tagaggttaa
     3661 aaaatgagct tatacccgaa agacaaaaat cccgtgtttc agcctggcgg ctacggtctg
     3721 cgctatgacg gtcaggccga ggccattgcg cgtgccggtg agccaccgat caactacgcc
     3781 ccgggtcagg agctgtcccg tgctgctcct gcgctgcctg agcacccgac gcatggcgct
     3841 gcgcgtgcgt tccgtgatca cctgacgggc gagtacgtcg tcgccgcggg cccggtcgag
     3901 gttcgtaccc gcgtatcggc tgaggaggcc gcgctgctgt gggctacttt gactgcgtaa
     3961 agctgaccga cgcggagtgg cctgatcaca tggttgaggg gcgtgatgcc gcccttcgcc
     4021 aggccatgcg tggtggcgtc tcccaatggt gccgggacaa ggttgtccag cttctcccgg
     4081 tgctgcggtg cccccggggc gagtggcgtg atgccgagct gaaacgtgta gacgagcttg
     4141 ctgatgccct ggggctggac gatatggggc tgtttgccta cggcgctatg atcgtgaagc
     4201 ggcgggctta cgttgctggt agtctctctc gggctaaacc gcccgagccg ctggctgttg
     4261 tcctcgatga tggcgagcat cgttgtactg tctatgcaat gccccacccg acgctcggcg
     4321 tgccggagat ctccgaacgc ggcgaggatg gatattggac tatctccgct ggcggttgcg
     4381 catggatcaa ggctcgcgtc tcgctcgggg aaggagtcag gctttggaac gagctggtta
     4441 attgcgcccg gtaccctgat gcgccggtcc tgacgcgggg gatgctggaa tgagcgagtt
     4501 tgctgtatcc cgtctggtga tcccccatgt ggtcatccct cccgccgttg tcgaggctcg
     4561 acgcgttcgc ggtgttgctg cagggcacgc cagaaagcgc cgacttgccg aggtcatggc
     4621 atcacctacc tggtactggt cgcggcgtga atctccccgt ctggccacca caaaaaaagg
     4681 ggcgtaagcc cctctctttt gggcgctgct gcgccgctac cggttgcccc agctgcttgg
     4741 ctggatgaac gtgccggaca tgctgacgct ctctggcggc ttgacctgct taccctcctt
     4801 gtcctcatga acgacgcgca ttgtgccgta catctcgccg acagcgcctt tgttgccgtt
     4861 gctgcccggg gtcatatcgt caacctgtgc cagatcgcgg tagtagctgt tcaccttgcc
     4921 ggggtaggcg acgttctctg cgctgccacg gcggctacct ccgttgtatc cgcgcaggat
     4981 ctcgtcctgt cccaggtatc cgtatcggtc acgcatctgg ctgtagacca tggcaccggc
     5041 catgatgttc tcgcgttcgt cgctgaggtt ggctcccggg gcgctggttg gcagtacctg
     5101 catgatgccc tgcgcacgtt tgctgctggt caggttgttg ttaaacccgg actcagtggc
     5161 ggcgatggcc tcaaggcgac gtggatcgat attgtacttg cgtccggcct cctcgaagta
     5221 agggcgcaga cgctccaggc gtttcttcat gtcgctgttg aggcggtatt tgccgccgcc
     5281 accgttggct gctgctgccg ctggtgactg gatgtatccg tcaccggtag acagcgccgc
     5341 ctctgtcgag gacgacgccc cgtatgtctg tagtttgatg cgttctgcgg ctctggtgcg
     5401 gtcagcccct tcctggtcgt tcaggctctg catctcggcc ttgagcttga ccagcttctc
     5461 cctgtccgga ccggaggact ggaggccgac gccttttgct gtcagggcat cgatctggct
     5521 ttggatggcg gcacggcggt ctttgtactt tgcgccgatc tcactgctgg cttctctcat
     5581 gttgctgtcg taatactgct tacgaacgct ggcttctgag ccgcctgtca tatttacaag
     5641 agccatgcgc atcatgtcga gggctggcag ggcgcgtttc gagtagtcct cgagggtgtt
     5701 gttgagtttg gcgatgttgt cgcggatctg gctcccctcg gtggtggtac cgccgtgctt
     5761 cgaggcgacc tctgcgagcg cgtctttgag cttctccggg tttgcgcctt taagggcgtc
     5821 gatcagcccg cgccgctcct ctttggacag gtcgctgcgc ttgaggtact ggttgccgag
     5881 tgcctccagg cctttgccgc tctcgatctg cccgaggacg gggagggagc tcatgttgat
     5941 gcttttctcg tcgataccga ggcgcttaag gcgggacgac gcgccgttga tgtccctgct
     6001 gctcatctgg tcgacgagca tggcctcgtt gacgccgatg ccgaggtggt tcgccagtgc
     6061 cttggtggcg ttggagccgt atccgtactg gcggtggatg tcatgacgga tcatgtcgag
     6121 catggtttcg ttgccgctga agctcttgcc ggtggtctgg ttgtagaggc tccccttgcc
     6181 cagcatgctg ctgacggtgg agaacgctcc cccctcctgc agtgcctgga gcgccatcgg
     6241 gtcattgatg ccccggcgca gtccgagacg ggcgctgaat gcctgtgacg cttcccccat
     6301 gttgccgccg tgcatgacgg ctccgttcat gcggtttaag aggctaccgg ctccctgcag
     6361 atccatgccc gggacgccgg aactcataag tccggccagt gcgccgccgt acccggcgat
     6421 atttggctgt gacaggctca tgcgtgcctg ggtgttggca aagtcggtga tcgccgtcat
     6481 tgcatcgcca gcctgtgcaa acgcgccaga cttggcgatt gcttccccca tcatcatgcc
     6541 ggttcgacgg atgctctggt cgctgttagt gacgcctgtc gctccgaggg aaccaaagga
     6601 ggcggcgaca ctgttcggat cgagtccgta accgcgtgcc agcccgccgc ttaccagtag
     6661 ccctgtccct aggttcatgc cggggcgcag attggatgcg cgggtgtagg tagacgccat
     6721 gccgatcgcg tctgcgttgt tcatgccgag acggttggcg atgtcgccga cgcctgaccg
     6781 caggttgctg tacgccacca tgccgccgac ctggcggtac agtttatcca gcccaatggc
     6841 gttgtcctgc gcctgtccga tcttatcagc gatagcgccg ataattttgc cgacgcccag
     6901 cgccgccaga ccgccgacaa gacccgccat gcccgcactc gcgccggaag acataccggt
     6961 gttcagggcg ttgctgatag taccgccaat agggccggca ttgccgagga tgctgtggag
     7021 gccactcatt ccctgtctcc ccagctcttt gcgccacgcg ggttctggcg gcgacggagg
     7081 cgctgggggc acgacaggcg gctttttggg gggctttggt gctggcaggt tgctgaactc
     7141 tacgcccgcc caccccagca cgctctccat gtacttgatt tgctctccga ggcgaccttc
     7201 ggccattttc ttgaagtcgg caaacagggg gttaccgccc tgcccggtct gtttcatttt
     7261 ggtggcaaag tcagtctgtt tcttcatgag caagtccatc tgcttgatga agccctgcag
     7321 gtcgtccttg ctcttgaggt tgatgggttc ccacttctgc ttgttggcgt tacctacctg
     7381 gctggccaga atgcggatct ggtcattgac cattttgatc tgctgttgga ggtcggcagc
     7441 gtcgaactgc gccgagaccg gaattttgat agccatagtt atctctctct gttgtaagcc
     7501 tcctcatgct ggcgtcacga cgtttggggt taaatgtcta aaacgtgtct aaaacgcaat
     7561 gcgtcgaaat cgacgcgcaa gccgctccac gtctggattc tttgaaaaat agaacttgta
     7621 aaatgtctaa aacgagtcta aaacagacac catccaatag cagttggata aacatggccc
     7681 ggatcgctcg ggtgcctgga cttcggtcta cttccggtgg cgcacttcca cctggtgaaa
     7741 agcgagtaag cgcgaatggc gcagccctgt cgctccggcg ataagcccat aaggactgca
     7801 tcgcaatagt catcactcaa agccccgacc actcacggcg gggcttttct acagggggtt
     7861 ccatgaagtt tcgtgcaagc gcgatctgcg acctgatggg tgtgtcccgt cgtcagctcg
     7921 atcgctggat caaggcgggc acctttcctc tgccggacgg gcgcgacgca aaggggtact
     7981 ggtgggaggc tgaccgggtg gccacgcgga tgatctccga acgcaaagaa cgggggatgt
     8041 gattatggtg aacgagagac gagattacct ttttgaccac gcggtgttct cggcggcgct
     8101 ggcgcgtgta gacccggatc cgtcggttat gggctggctg gctcaggctc gcacgctggc
     8161 gtgctggtgt gaactgctgg ggatggatcc gctggagacc tgtgtggagt ggtctgcgcg
     8221 tggtgtgaat gcgggaaagg gcgacagggc tatgttggtg tcggcgctgc gtgacctgga
     8281 tgaactcgac cggctggcgg ctgctggggc gttcctgcgc tcctccgggg tcgctgctga
     8341 cgttgccgcg gggcgtattg ctgtgcgtga gccgtccgag gacgagctgg cggctcgcca
     8401 ggcgcatctg gttgcgttac gggaacgtgc ggcggctgct gcggagaagc tgacggaggc
     8461 tcgcattaag gcaaccggta gggcgcggat gctgctgcat cgcgcaacca aaccagggag
     8521 tgctgcgctg tactggcaag ccaaagagcg ggcggcgtgt ctccctttgc cgtgcccgga
     8581 tgacgtgaag tgctcggact ggcgcacgtt cctgcctgta atcggtcagg tgtactggaa
     8641 cagctcgctc ctggtgccgc tgtacggggt gaatgagtcc ctgcggctgg agcagatgtc
     8701 ggtcgaggtg atttgcggtc gggctaaccc ggaggcggcg ggttcgctgg gcgagaagcg
     8761 cgggctcaag aatgcgccgc gtgaggggct gttctacccg ttcgatctgg atgctgtcgg
     8821 ttccggggct cctgtggtgg tctgcgaggg gttcatgtcg gggctggcgc tctccctgct
     8881 gattggcggc aagctcccgg tggtctgcgc gatgtcggtg ggtaacttcg gggcaacggt
     8941 gcagtcgctg aataagtttg ttatgggggg ccacccgttc gtactggtac cggacattgg
     9001 cgggcaggat ctggctatcg atcaggctat cccgaatgcc cgggtgatcg acctgtcggc
     9061 ggtaccggga gccgagggcg tggacggcta cgacccgttc gatctgctgg cgcgtcacgg
     9121 cgtcgagatg ggtcgcaagt acctgcgggg cttgtttcgg gaggcgcgtg acgcgagcct
     9181 gtagtggtac cagttttctc tctcgttgtg cccagcccgc ttcagcgggc ttttatttga
     9241 ggatctcccc atgaccgacc agaccaccac cgtcgccgac ctggcgctct cacaatccgc
     9301 tttcctcgtc tctcaggcta tcgtcgagct ggacgccatg ttcggcgagg gctatgccgc
     9361 tgctcacccg gctgtgctgg ctcaattcct gcagacggcg ggggtgatct acgccacgca
     9421 gctgcaggct actgccgcgt tcgacctcgc ccaacagctc gatcgcctga tcggccttgg
     9481 gctgggtgtt gaggctggct tgctgcaagc ggcgcagggt gcctcgcact aaaacgccgg
     9541 ggccggctgc gatcagtcct ttgccctggg cggcggctgc cgcgctaccg gttacttcag
     9601 gtcaggcatc cggcgggtga tcaccgtgtt gatgcggtcg ttgttccatg ggcgaccgtt
     9661 cgactgcagg agccctgcct cgttcagcgc ctgggcgata gctttgttgt cgtagcggaa
     9721 tccccgatcg cgctgctcct ccctcatgcg ccgtatggtg gccatcacct cggcctcgtc
     9781 gtaatgcccc gaaatttcag cgccagcggg gcgctgtgct gcgccttgtg tttcagatga
     9841 gttactactc agcttttcag cccgtggcct tgtagcgcgt cctgttggct tctgtagcac
     9901 gttgtgtttc agcgtattcg agatgtcctg cagcagctcc accatcaggt cgaggcggtt
     9961 gccgctctcc ccctgcggct ctggtatgcc aaggtatgcg cggagtgcgt cggtaatcac
    10021 gtcggtgcgg gtacggttcg ttttctcggc cagctcgttc gtctgctcga gcaggtcggc
    10081 gggtagacgt aggctgaatg tcgggctttt gcgctgcatc cgtgttgctc ctgtgttgtc
    10141 tggtgccacc gattgaaaca cgtttgtaac ctcgcgtcta tttcgacgtg ttgcgcgtgt
    10201 gctgcataac cggtacgggt cgagcccttc ggctcgcctc tctggtgcct gtggacaagc
    10261 cctgtggaca gtttgacgtt gcagtggata aaagcgcggc gcgatcacga cagctcgata
    10321 agcggtcgcc gttcagtggc atgaagggtt aggatcggtt aagctgatcg tcgttcgtgg
    10381 atcgtgttgg gtgatctgcg gatacaaaaa aagcacctgg ccgggtgctt tgattgccgg
    10441 ggttaaaccc cgtagtttcg agtcagtgtc ttaacgggac gaactcgaag gaaatcatac
    10501 atcacgttgg gtgattctca aatcctcgct gagaattgta tcacgtcgat aagatcgcgc
    10561 aagtgtgaat ttccctcgta agcccccgcc tgacgccgac tcctacgttt ttaaaggagt
    10621 aattgcgtgt cacaaattga tgaaaattcc ggcactgctg ccgacaacgt aacgccgatc
    10681 gccgctgcca aggtgggacg cccgtctcta caaaaaaaat acccgactac cgcgtttccc
    10741 gtggagctgg agatcaacta ccggaagggg cgctactcgt cctctcttga gaacgttgtc
    10801 gccgctatgc gctgccattc cgtcaccggc atccagctcg ctttcgatga gttctttgcg
    10861 gaggaggggg cgttcgtgaa cgggcaatgg tacccgctgt ccgacaacct agttaccgag
    10921 ctgcgtctgc gcctcgctgc tgccgacttt gagaaggtga ccaaggacaa tgcccgcgac
    10981 gccatgttct acgtctgcga tgagaacaaa ttcgacagcg cccagcgttg gctgacggct
    11041 caggaatggg acggcaaacc tcgcgtcagt tacttcttcg agcggtgctt caaatgccaa
    11101 ggggatcccg agtacctggc ggcggtcagc ctctacacct ggacggcgct tgccgggcgc
    11161 gtaatgaatc ccggaatcaa ggccgacatg gttccgctgc tgtacgggcg acagggtgcc
    11221 cgcaaatcga cgggcgttcg ccttatgtcg ccattccctg actcctactc cagcttcaac
    11281 ctgaacgaca aggacgactc cctagtgcgc cgtatgcgtg ggtgcctgat tggcgagctg
    11341 gaggagctgc gcggcctcca cgggcgcgac tccgaatcgg tgaaagcgtt cttctccgct
    11401 gcccacgacg agtgggtgcc gaaatttaaa gagttcaagg tgcgaaaccc gcgccgcgtg
    11461 gtgttcatcg gtaccacgaa caacaagcag atcctcaatg actcgacggg gaaccgccgc
    11521 tatttgccca tgaattccgg ggacgtggac tgcgcggcca tcgagaaaaa ccgctcgcag
    11581 ctgtgggctg aggcgctcca cctgttcaat gagaatgggg tgatgtggga ggctgctgaa
    11641 acgctggcgc gtaaggtgca tgacgagttc cgcgtcgagg atccgtggga agacgagatc
    11701 gccgagctgc tcaagatcgg ggtcgacgac tgcgaaccga ccagcttcgt gccgactaag
    11761 gcgttgttct ctcaggtagg gctcatgatt ggccatacca gcaacaagca gcatcagcgc
    11821 atggcggcta tcatgtcccg attgggctat gaggttggca ggaagcgcat aaacggtgcg
    11881 ttagttcgtg gctacttccg tcgaacgcaa gctgtcgtgt aagggttact tttccgtaag
    11941 aggggagcta tatgctcccc tttttttatt gatccacccg ggtggatcac gggtggatca
    12001 tcacgccgtt tttgtaccac ctgttccacc cgggtgttcc acccgttcca cccatgttcc
    12061 acccgtgggt ggaacagtta atgccgcgta tttaaaggct ttcaccgttg tgttccaccc
    12121 gttccaccct aatttctaat taaatacaaa aaatgaataa attggttaaa tagggggtaa
    12181 atgctcacta attgaacaat taattgtttt ttttactatt tatgaacctg tgccggatcg
    12241 ggtggaacgg gtggatcatc tggaaatcga cgcgccgcag ctgcaccccg ctggggatgc
    12301 actttgcgtg tcgtgtttgg atctggtgtc gctctcaccg gtgcgtggtt tgcacctgcc
    12361 ggatctccag ctaaaaaagc ccggagggaa atggggccag tttccagtgc tggcgggggt
    12421 tggcgggtgg tttatgcacg gtggatcatg gttggcaccg tgcattgccc ttaggcggca
    12481 tcgccgcgcc taccggttcg tcaaatcctg ccctaacttt cagggggtac acggggttaa
    12541 atggggtgtt gccgtgtcgt tgggtt
//
