LOCUS       NZ_AP022092             6796 bp    DNA     circular CON 14-JUN-2023
DEFINITION  Escherichia coli strain WP4-S18-ESBL-07 plasmid pWP4-S18-ESBL-07_5,
            complete sequence.
ACCESSION   NZ_AP022092
VERSION     NZ_AP022092.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194481
            Sequence Read Archive: DRR199317
            Assembly: GCF_014171015.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Escherichia coli str. WP4-S18-ESBL-07
            isolated from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6796)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 166x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/14/2023 05:36:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,281
            CDSs (total)                      :: 5,159
            Genes (coding)                    :: 4,963
            CDSs (with protein)               :: 4,963
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 84 of 196
            Pseudo Genes (incomplete)         :: 130 of 196
            Pseudo Genes (internal stop)      :: 43 of 196
            Pseudo Genes (multiple problems)  :: 49 of 196
            Pseudo Genes (short protein)      :: 4 of 196
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022092.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="WP4-S18-ESBL-07"
                     /isolation_source="wastewater"
                     /db_xref="taxon:562"
                     /plasmid="pWP4-S18-ESBL-07_5"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6514 N 139.4813 E"
                     /collection_date="2018-08-06"
     gene            complement(837..1028)
                     /locus_tag="H7R53_RS26010"
                     /old_locus_tag="WP4S18E07_P50010"
     CDS             complement(837..1028)
                     /locus_tag="H7R53_RS26010"
                     /old_locus_tag="WP4S18E07_P50010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_064767211.1"
                     /translation="MTKQEKTALNMARFIRNQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1092..1415
                     /locus_tag="H7R53_RS26015"
                     /old_locus_tag="WP4S18E07_P50020"
     CDS             1092..1415
                     /locus_tag="H7R53_RS26015"
                     /old_locus_tag="WP4S18E07_P50020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_079774495.1"
                     /translation="MLTIRVTDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     APPLLRHLAAIGNNLNQTARKVNSGQWSSIDRVHVVAALMAIEGELRQLRQAVREQGVR
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1624..>2061
                     /locus_tag="H7R53_RS26545"
                     /pseudo=""
     CDS             1624..>2061
                     /locus_tag="H7R53_RS26545"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2093..2653
                     /locus_tag="H7R53_RS26025"
                     /old_locus_tag="WP4S18E07_P50030"
     CDS             2093..2653
                     /locus_tag="H7R53_RS26025"
                     /old_locus_tag="WP4S18E07_P50030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_137498897.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISGHTTSVKQQLDTLSTTVSTQFSTTEAELSRQQKNLLWQVIKGRILFPSLTA
                     LSVTGGIFLGCWGLIQWQESRIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGVKGENTWTVGDKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            2660..2893
                     /locus_tag="H7R53_RS26030"
                     /old_locus_tag="WP4S18E07_P50040"
     CDS             2660..2893
                     /locus_tag="H7R53_RS26030"
                     /old_locus_tag="WP4S18E07_P50040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_059331885.1"
                     /translation="MTELERHLLNALEHLQQDYTQRLKDWESAFTDLQHMFERTRQENG
                     QLREQCDNLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4043..5011)
                     /gene="kdgT"
                     /locus_tag="H7R53_RS26035"
                     /old_locus_tag="WP4S18E07_P50050"
     CDS             complement(4043..5011)
                     /gene="kdgT"
                     /locus_tag="H7R53_RS26035"
                     /old_locus_tag="WP4S18E07_P50050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006810427.1"
                     /GO_function="GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA]"
                     /GO_process="GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="WP_059331886.1"
                     /translation="MKIKATIEKIPGGLMLVPLLLGAILNTFAPETGNYFGGFTKGLIG
                     GTVPILAVWFFCIGASISLKASGIVLKKSGTLVLTKIAVAWLVAFVCTKFIPSDGIQTG
                     FFAGLSVLAIVSAMDMTNGGLYASLMNQYGSKEEAGAFILTSLESGPLMSMIILGTAGL
                     ASFEPQHFIGAVLPFLIGFLLGNLDEELKEFFTKANQVLIPFFGFALGNAINLGVILDT
                     GLLGIILGLAVIVITGIPLILADRIIGGGNGTAGVAASSTAGAAVANPVLIAQINPIFE
                     PVAASATALVATCVVVTAILVPIITALYAKHISPKMKTKTL"
                     /name="kdgT"
     gene            5210..6232
                     /locus_tag="H7R53_RS26040"
                     /old_locus_tag="WP4S18E07_P50060"
     CDS             5210..6232
                     /locus_tag="H7R53_RS26040"
                     /old_locus_tag="WP4S18E07_P50060"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]; GO:0043565 - sequence-specific DNA
                     binding [Evidence IEA]; GO:0000976 - transcription
                     cis-regulatory region binding [Evidence IEA]"
                     /GO_process="GO:0006351 - DNA-templated transcription
                     [Evidence IEA]; GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]; GO:0006355 -
                     regulation of DNA-templated transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="WP_137498896.1"
                     /translation="MNKSSSISDVAQLAKVGKTSVSRYLNGEHHLLSEKLKERIKNAIE
                     VLDYTPNPLARSLKNKRSKLIGLLLADINNPYSVRIINSVEKYCWENDFVPIVFNTDNS
                     FEKESKALDILNRYQVEGVVINTVNSSKPFGEYRFPVVLIDRKIPGKLFDMVGLDNYHA
                     VNTAVQHLVNEGFDDILFISEPVMGVHTRLERQRAFYNSSRCFHDIQYHAQEIGCLDID
                     GIEKILLTFCDKRINAKRKAIISANGALTISLIKVINYLNIRIGVDVGFLGVDDVEWSE
                     IIGGGITIIKQPTDLIGSAAMELLHSRIDTPDIPSRDAIFHGDLIIRQSTINLSRFQSK
                     "
                     /name="LacI family DNA-binding transcriptional regulator"
     Typing          672..756
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.209"
                     /coverage="99.0"
                     /evalue="2.41e-18"
     Typing          complement(102..661)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.118"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1063..1409
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="86.207"
                     /coverage="98.0"
                     /evalue="4.09e-59"
     Typing          1406..2970
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.564"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tagttgggct atcacttcaa gaactctgta agcatctgga taaatctcgc tctgctaatc
       61 cgattaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggattcaaga
      121 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
      181 agcttggagc gaacgaccta caccgagccg agataccaac agcgtgagct atgagaaagc
      241 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
      301 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
      361 tttcgccacc tctgacttga gcgtcggttt ttgtgatgct cgtcaggggg gcggagccta
      421 tggaaaaacg ccagcggcgc ggcctttttc cttggctttg tcttgttgcg cacatgttct
      481 ttccggcgtt atcccctgat tctgtggata accgcatcac cgtctttgag tgagctgata
      541 ccgctcgccg cagccgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaaaagt
      601 gcccggatgt gcattttctc cttacgcatc tgtgcggcat ttcacaccgg acatggcgca
      661 cttctcatac aatccgcagc aatgcaaaat gagataagcc agtatacaca tcgctatcgc
      721 tctgtgactg ggtcagggct gcgccccgac acccgcactg acgcgccctg gcgggcttgt
      781 ctgctcccgg catccgctca caggcaagct gtgaacgtct ccgggagctg catgtttcag
      841 ttgctttcac cgtcatcgcc gaagcgtgcg aggcaactgc ggtagagctc gtcggcgtgg
      901 tcgtgaagag attcgcagat gtctgcctgc tcgtccgcgt ccagctcgtt gagtttttcc
      961 agcagggtca gcgtctggtt tctgataaag cgagccatgt taagggctgt tttttcctgt
     1021 ttggtcatgg tcctaatgct ccttgtatgg gggatttgtc agggggaatg ataccgatga
     1081 aacgcgagaa gatgctcacg atacgggtta ccgatgagga gcacgcccgg ttactggaac
     1141 gctgcgaggg taaacgactg gcggaatgga tgcggcgggt ctgtctgggg gagccggttg
     1201 cacgcacagg aaaactgccg acactggcac cgccattgct gcggcatctg gctgccatcg
     1261 ggaacaacct gaaccagaca gcccggaagg tgaacagcgg ccagtggtct tcgattgacc
     1321 gggttcatgt ggtggccgca ctgatggcca tcgagggtga attgcgccag ttgcggcagg
     1381 ctgtcaggga acagggggta cgggatgatc gttaaatttc atgccagggg gaaaggcggt
     1441 ggaagtggcc cggtcgatta cctgctgggg cgggagcgta accgcgaagg cgcaacggtg
     1501 ctccggggta acccggaaga agtgcgggaa ctcattgatt ccacgccatt ttcgaagaaa
     1561 tacacttcag gcgttctgtc gttcgcggag aaggagttac cgccgggtga acgcgaaaga
     1621 gtgatgacga gctttgagcg tgttcttatg cccggtcttg aaaaagacca gtacagcatt
     1681 ctctgggtgg aacaccagga taaggggcgg ctcgaactga attttgtcat cccgaacatg
     1741 gagctacaga gcggtaagcg cctccagccg tactacgacc gtgcggacag gccgaggata
     1801 aatgcctggc agacgctggt taatcatcat tacgggctgc gtgatccgaa cgcaccggag
     1861 aacagacgga cgcttgtcac gccaaataat ctgccaaaag cgaaacagga ggccgcagaa
     1921 gcgattacac ggggtcttga ggctctttac catgccggag caataaaaac gcgtcaggat
     1981 gtcacagagg cgcttacagc ggcaggtttt gaggtggtga gaacaaccag aagcagcatc
     2041 agtattgccg acccggaggg ggggcggaat ctcaggctaa agggggcgct gtatgagcaa
     2101 tcttttacaa atggggacag acttcgagaa gaaactgaaa gagcgagccg cttctaccga
     2161 gaacatgctg aacagcgagt tcaggaagct cgaagagtct gtagacaagg cactgagctt
     2221 aaacaggcag aaaatcagag acgctatcag cggacatacg acatccgtga aacagcagct
     2281 ggatacgctg agcaccaccg tgagcacgca gttcagcacc acagaggccg aactgtcgcg
     2341 gcagcagaaa aacctgctct ggcaggtgat caaaggacgg atactgtttc cgtcgctgac
     2401 cgcgctcagc gtcacgggcg gcatttttct ggggtgctgg gggctgattc agtggcagga
     2461 aagcaggatt gcgaaaaata tcctgaccat cagagagcag gaaaacacgc tggcgaaact
     2521 cgaggcaaaa acctgggggg tgacgttcgt caatggcgag aacgggaaat ttctggtgtt
     2581 gccggacggg gtgaaagggg aaaacacctg gacggtgggc gacaagaacg ccgtgaggct
     2641 ggtcagggag taaacgagaa tgacggaact ggaaaggcat ttgctgaacg ccttagagca
     2701 tttgcaacag gactatacgc agcgactgaa agactgggag agcgccttca cggatttgca
     2761 gcatatgttc gagcggacac gacaggagaa cgggcaactg agggaacagt gcgacaactt
     2821 gagtcagcag gtgcagagat tagccgggca ggtggaccgc ttgagtcggt tatttatcac
     2881 gaacagcaga taagggatga gcaggatcgt gcccggtaca gatataagat acaggaacgc
     2941 gtggcgcgga gtcggagtgg tccgtcaatg gagctctaaa aaactccccc taacgggggt
     3001 ctccaacaga gaagtctgag tacgcatgaa aaaatttggg ccggtcatgg accactcaag
     3061 aggaatgcgc attttgcgcg atgtaacgga atcaatttgt cagataaccg taaaaaaaac
     3121 aaggttaagt ccagccaaaa tgggaaacat catgttatgt tcatgtggcg ttcacataac
     3181 aacaaataat ctccgcatag tcttttcgcc cccctgctcc aaggggtttt tttttgagcg
     3241 cgcttctgac accaaccctg tttcccatcc ttaaatggca tcaaaaacgc gctcagacgc
     3301 gttctaacgt gattttcagg accgggcaag tttggattga gccccgtatt catagctcgc
     3361 gaaaaatttc gcgccccgct cactgtttcc aatgagcaaa cttgccattc gcttacatcc
     3421 cccccaacga caaagcctgt cgagtcttcc cgcaaacccg atccatttac cataactccc
     3481 gttgcgcgca ccgctaaaac acgttcggtg cgatcacggc aacagacagg taaaaccagc
     3541 aacaaaccac ccgaaaagtt gccgcgatcg cgcctggtaa attttaacct catgcatagt
     3601 tatgcgcgaa actgaatgac gatcagtttc gcacgttcca gccgtcgaac gggtcaaaat
     3661 ggcaaggttt ctcggttact ggcgatatgg atgggttgtg ttttttgttg aattgcggca
     3721 aattcaggac gcagagtagc gtagttaaac aatcacatta ttactttttg cccgttcttc
     3781 cagatgggct tttttttgtc cgtttgaacg caggcagaac agacagcccg ggcaaaagct
     3841 gccgcagaag cacaggctaa agcaaaagaa aaccgggagg ggctgcggct ccatcatctc
     3901 cgtctaaagt atatttatgt taaataagtc tttgctgtag ggggcggtga ggattttatc
     3961 gtgtctggaa tcagtactct tataagccag acaaaaagcg ccaggctgac gctttttgtc
     4021 ctaacgaaat gttcagctgc aactaaagag ttttagtttt catctttggt gatatgtgtt
     4081 ttgcatataa ggccgtaata attggcacta aaatagcggt aacaacaacg catgttgcca
     4141 ctaatgctgt agctgatgct gcaacaggct caaatattgg attaatctgt gcaattaaaa
     4201 ctgggtttgc taccgcagca cctgctgtgg acgaggctgc cacaccagca gtgccattcc
     4261 cccctccaat aatacgatcc gccaatatta atggtattcc ggtaatgaca ataaccgcca
     4321 gtccaagaat tatacctaat aatcctgtat ctaaaatcac gccaagatta atcgcattac
     4381 ctaacgcgaa tccaaagaaa gggatcagga cttggttggc tttcgtgaag aattctttca
     4441 attcttcatc taagttaccg agtaaaaagc caatgagaaa tggtaatact gcaccaatga
     4501 aatgttgagg ttcaaaggat gcaagtcctg ctgttcccag aataatcatc gacatcaatg
     4561 gccctgattc caaagatgtt aagataaatg ctccagcttc ttctttactt ccatattgat
     4621 tcattaggct ggcatatagg ccaccattcg tcatatccat ggcggataca atagctaaaa
     4681 cagacagacc tgcgaaaaaa ccggtctgga taccatcact tggaatgaat ttggtacaaa
     4741 cgaacgcaac taaccaggct actgcaattt ttgtcaaaac caatgtacct gattttttta
     4801 gaacaatacc ggaagctttt aggcttattg aggcaccgat acaaaaaaac catacagcta
     4861 atattggtac tgtgcctcct attaagcctt tagtgaaacc gccaaaataa ttgcctgtct
     4921 ctggggcaaa ggtgttcaat atcgcaccaa gcagtaatgg tacaagcatt aaaccacctg
     4981 ggattttttc tattgttgct ttgattttca taatgcattc ctttgaattg ttttctttag
     5041 ttcatcatgg aaccgattcc agtgcatcat ggaaaatata gttatgtgaa tcgtgtcaaa
     5101 aaaaaagtaa tccgcaaaat catatattcc aatggatgtg agtgattgtc atgatgtgta
     5161 taacaaattg gaataagtgg tatgccagcc tgaacatagg gtatcaaaaa tgaataaaag
     5221 tagttctatc tctgatgttg cccaactagc aaaagttggc aagacaagtg tgtctcgata
     5281 tctcaatggc gaacatcatt tgttatcaga aaaactcaag gagcgtataa aaaatgcgat
     5341 agaggttctt gattatactc caaatccctt agcaagaagc ctaaagaata aacgaagtaa
     5401 acttataggg cttttactgg ctgatataaa taacccatat tctgtaagaa tcattaatag
     5461 tgttgaaaaa tattgctggg aaaatgactt tgttcctatt gtctttaata cagacaatag
     5521 ttttgaaaaa gaaagtaaag ctcttgatat attaaataga tatcaggtag agggcgttgt
     5581 aattaatacc gtcaattctt caaaaccatt tggtgagtat cgctttccgg tggtcttaat
     5641 tgatagaaaa ataccaggaa aattattcga tatggtaggg cttgataatt atcatgccgt
     5701 aaatacagcc gttcaacatc ttgtgaacga gggttttgat gatatactat ttattagtga
     5761 gccagttatg ggagtgcata caaggcttga gcggcaacgt gcattttata attcatcaag
     5821 atgttttcac gatattcaat atcatgcgca agaaataggg tgtttggata ttgatggtat
     5881 tgagaaaatt ctacttacct tttgtgataa aagaataaac gcaaaaagaa aagctattat
     5941 atcagcaaac ggcgcattaa ctataagtct aataaaggta attaactatc ttaatataag
     6001 aataggtgtt gatgtcggtt ttctgggagt tgatgatgtt gaatggtctg aaattattgg
     6061 cgggggaatt actataataa agcaaccaac agatcttata gggagtgcag cgatggaatt
     6121 gctgcactcc agaatagata ctcctgatat tccttcacgg gatgctatat tccatggcga
     6181 tttgataatt cggcaatcta ctataaactt aagtaggttt caatctaagt aaatatgcta
     6241 atgccttaat aaattgatga ggcttatctc tgaacggtag ataatcttga ttagatgctt
     6301 tggaggagag aacgatagct gataaccgat tatctctcct cgtgatttat atctatgtgt
     6361 gaatgttagt tcgtgtgatg ttgttgagtt gttcaataga actgttactg gttattaagg
     6421 catctaaccg gatacataag agattgcgtc attttctcca ttgaagatag actcccccac
     6481 tgttaagtgg aggtaaaaag gtaattaata caatttgcta cgcaatacac atcgttatgt
     6541 tggtggttat aggtgctact cggttatggg gaggggtaac attatctttg tcgctgacat
     6601 agatggtggt ttggtgaaaa aagatcagga gatcctcttg tgatcctttt tttctgcgcg
     6661 taatctgctg cctgtaaacg aaaaaaccgc ctggggaggc ggtttagtcg aaggttaagt
     6721 cagttggggc tgcttaaccg aggtaactgg ctttagcaga gcgcagatac caaattctgt
     6781 ccttccagtg tagccg
//
