LOCUS       NZ_AP022062             3484 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella sp. WP4-W18-ESBL-05 plasmid pWP4-W18-ESBL-05_5, complete
            sequence.
ACCESSION   NZ_AP022062
VERSION     NZ_AP022062.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194464
            Sequence Read Archive: DRR199300
            Assembly: GCF_014169075.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella sp. WP4-W18-ESBL-05
  ORGANISM  Klebsiella sp. WP4-W18-ESBL-05
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Klebsiella sp. WP4-W18-ESBL-05 isolated
            from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 324x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 10:05:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,069
            CDSs (total)                      :: 4,953
            Genes (coding)                    :: 4,803
            CDSs (with protein)               :: 4,803
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 36 of 150
            Pseudo Genes (incomplete)         :: 116 of 150
            Pseudo Genes (internal stop)      :: 17 of 150
            Pseudo Genes (multiple problems)  :: 17 of 150
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022062.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Klebsiella sp. WP4-W18-ESBL-05"
                     /mol_type="genomic DNA"
                     /strain="WP4-W18-ESBL-05"
                     /isolation_source="wastewater"
                     /db_xref="taxon:2675713"
                     /plasmid="pWP4-W18-ESBL-05_5"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6514 N 139.4813 E"
                     /collection_date="2018-02-13"
     gene            complement(101..370)
                     /locus_tag="H7R58_RS25165"
                     /old_locus_tag="WP4W18E05_P50010"
     CDS             complement(101..370)
                     /locus_tag="H7R58_RS25165"
                     /old_locus_tag="WP4W18E05_P50010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_109233449.1"
                     /translation="MSKSTEQQIAELQNRLQALRTKKARQDKREETRQKIILGAEVAKV
                     LNSKPEEVNKALLLGLLSKINDLDDEEKSEFTRLGMDILQQWKS"
                     /name="conjugal transfer protein TraD"
     gene            complement(376..660)
                     /locus_tag="H7R58_RS25170"
                     /old_locus_tag="WP4W18E05_P50020"
     CDS             complement(376..660)
                     /locus_tag="H7R58_RS25170"
                     /old_locus_tag="WP4W18E05_P50020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_109187434.1"
                     /translation="MNYQHKNLLSDEEKAFYQKYFEKALEDLLEKKNKEIKEEFEKITN
                     DLILEEVDRFINIHGASITNRHNFFKRDYFASREIFENVFNDRINKNRW"
                     /name="hypothetical protein"
     gene            complement(662..991)
                     /locus_tag="H7R58_RS25175"
                     /old_locus_tag="WP4W18E05_P50030"
     CDS             complement(662..991)
                     /locus_tag="H7R58_RS25175"
                     /old_locus_tag="WP4W18E05_P50030"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_109233450.1"
                     /translation="MPRLEQKEYLELFDKIFKEHSYMYEENRCHYDRFIYDVFNFIGDN
                     LEEEITKELEIYKGSEDYDYYYDSFYTVLFQSEMAMVEPILKDYLKNKIFSVENGYLNF
                     DCVEG"
                     /name="hypothetical protein"
     gene            1221..2381
                     /gene="mobQ"
                     /locus_tag="H7R58_RS25180"
                     /old_locus_tag="WP4W18E05_P50040"
     CDS             1221..2381
                     /gene="mobQ"
                     /locus_tag="H7R58_RS25180"
                     /old_locus_tag="WP4W18E05_P50040"
                     /inference="COORDINATES: protein motif:HMM:NF041496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_109233451.1"
                     /translation="MAIYHLSFKLCKRSEGKSSVYLAAYQNRNKFTDNRTGAVWNYSKK
                     EGFDGSLILAPPEAPGWLTESSEALWNEVERTERQKNGQTARYFDVALPVELDDKQKKD
                     LLIRYCQENFVNEGMIADIAFHDLNSKNPHAHVMLTMKNVGPEGFLTKNREWNDKKCLE
                     AWRQNWEVITNEALKKYGHKSRIDCRTLEEQKEVAERKAVFAKTPQAKAVWIAQSIRLD
                     RTPMTRIHRNNWKEGQKLRKMEQEEKRLLYKKAALTYHKMTKPIEPEQSMNIQKTPIAQ
                     KIKSIFETAKKKLNSKIADFFKHKTKKETPEKFVSSYEIDDLGNHILKKEAENWDQKNN
                     DKIKKQVREKKEKEESERLRLKEEDQKLKNSSKPIQQNTSTRKLKR"
                     /name="mobQ"
     gene            complement(2413..2562)
                     /locus_tag="H7R58_RS25185"
     CDS             complement(2413..2562)
                     /locus_tag="H7R58_RS25185"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162556323.1"
                     /translation="MKKKKLQKKKRRNKNKHSKFFKMLIKSVEEAVKIHKGELEPARVT
                     TLKM"
                     /name="hypothetical protein"
     Typing          1222..2378
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.896"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 caccagaggc cctctgagag ctttttaaag cctccaggca agcctggcaa cccttcacag
       61 taaaaaacca gccgatggct ggttcttcac tgttcacggt ttacgatttc cattgctgca
      121 atatgtccat tccaagcctg gtaaattctg atttttcctc gtcgtctaaa tcattgattt
      181 tactgagcaa acctaataac agagctttat taacttcttc tggtttacta tttaaaactt
      241 ttgcaacctc tgcacccaat attatttttt gtctggtttc ttctctttta tcctgccttg
      301 ctttttttgt tcttaatgct tgtagtctat tttgtaattc tgcgatttgt tgttctgttg
      361 atttactcat attatttacc acctgttttt atttatacga tcattaaaca cattctcaaa
      421 tatttctctt gaagcgaaat aatcgcgttt aaaaaagtta tgcctgtttg ttatacttgc
      481 accgtggata tttataaacc tatcaacttc ctcaagaatt aaatcattag ttattttttc
      541 aaactcctct ttaatttctt tattcttttt ttctaataaa tcttcaagtg ctttttcaaa
      601 atatttttga taaaaagctt tttcctcgtc gcttaataaa ttcttatgtt gataattcat
      661 attaaccctc cacacagtca aaatttaaat atccattttc cacagaaaaa attttatttt
      721 ttagatagtc ttttagaatt ggttctacca ttgccatttc actttgaaaa agcacagtat
      781 aaaaactatc ataatagtaa tcgtaatcct cagatccttt atatatttca agttcttttg
      841 ttatttcctc ttctaaatta tccccgataa aattaaaaac atcataaata aatctatcgt
      901 aatgacatct attttcttcg tacatataac tatgctcttt aaatatttta tcaaacaatt
      961 ctaaatattc tttttgttct aatctcggca tattaaacct cctatattta ttttattatt
     1021 taatacctcc gttatttact ttagtcaaat ataatttaat atatttatat atattgggcg
     1081 aagccgtttt attagttcat tatctttttg agccgtggcg aaaattagat aatgcccact
     1141 tatacactcg ttccgagtgt gtgggccctg cggggctaaa acccaaaatt caaaagataa
     1201 aaaaagtaag ggggtagcct atggctatat atcatttaag ttttaaattg tgcaaaaggt
     1261 cagagggtaa atcttccgtc tatttagctg catatcagaa tagaaataaa tttaccgata
     1321 atagaacggg tgccgtgtgg aattacagca aaaaagaagg ctttgacggt tcgttaatcc
     1381 tggcacctcc agaggctcct ggttggctca cagagtcctc tgaggccctt tggaatgagg
     1441 tagagaggac agaacgacag aagaacggac agacagccag gtactttgat gttgctctgc
     1501 cggtggagct ggacgacaag caaaaaaaag atctccttat aaggtattgc caagagaatt
     1561 tcgtaaatga gggaatgatt gccgatattg catttcacga tctcaatagt aaaaatcctc
     1621 atgcccatgt aatgttaaca atgaaaaatg taggaccaga gggattttta acaaaaaaca
     1681 gagaatggaa tgataagaaa tgtttagaag cctggaggca aaattgggaa gttattacca
     1741 atgaggcatt aaaaaaatat ggtcataaat cccgtattga ttgccgaacc ctggaagaac
     1801 aaaaagaagt ggcagaaaga aaggctgttt ttgctaaaac tcctcaagca aaagctgtat
     1861 ggattgctca atctatccgt ttggacagaa cgccaatgac ccggattcat aggaacaatt
     1921 ggaaagaggg gcagaaatta agaaaaatgg agcaagaaga aaagagactt ttatataaaa
     1981 aggcagcatt aacatatcat aaaatgacca agccaataga gccagaacaa tcaatgaata
     2041 tacaaaaaac tcctattgct caaaaaataa aatctatatt cgaaactgct aaaaagaaat
     2101 taaattcgaa aatcgctgat ttcttcaagc ataaaacaaa aaaagaaact cctgaaaaat
     2161 ttgtcagtag ttatgaaatt gacgacttag gaaatcacat attgaaaaaa gaggctgaaa
     2221 attgggatca gaaaaacaat gataaaatta aaaaacaggt tcgtgagaaa aaagaaaaag
     2281 aagaatcaga aaggttgagg cttaaagaag aggatcagaa attaaaaaat agttctaagc
     2341 caatccagca aaataccagc accaggaaat taaaaagata gtatatatta ctatatacta
     2401 caatatacta tactacattt tcaaagttgt taccctggct ggctctaatt ctcctttgtg
     2461 aattttcacg gcttcctcta ctgacttaat aagcatttta aaaaatttac tatgcttatt
     2521 tttatttctt ctttttttct tctgcaattt cttttttttc ataatttcac caaatattcc
     2581 aaatcgccaa ttttttcttc gaactatttt tttttattct ctagtactgt ttttctatat
     2641 tattttaatg cttcgcacaa tataaaagat cagtattgtt tttctgtgct tcgcgtaatg
     2701 ctcaccttgt gagaacccca gcttcgctgg gcttcaccca aggttccgcc tcccagattt
     2761 ttcgagtaaa ttcagccatt tagactgcta aactcaaaaa aaatctgaga gacgatcctg
     2821 gtatgtgagc attatttttg gcttcaccca agagccagga cttagagata tataaacttt
     2881 cgtctatatg gctccctgct acactttcct ggtcgctcca tataatccat ttgcttcaat
     2941 ctaaccccat tttaaaccct atctaagcgg ttctggctgg gctttctcat agcctacctg
     3001 attgataacc cggacaactt aggaactcgt tccaagttcg gcaattcgta gcattccccg
     3061 tctttgccta caaccaacac gacccggaca atcacattgc attccacaga tcgaggcacg
     3121 acacaatatg caagtttggt tgcttaagga cttgaaaagg atggcttttt ttggggtgtt
     3181 gcaatcgaag ggtatttgac tttttgcgaa ttgtaggtta agtttctact tacagttcgt
     3241 aatccagtgg tctcaacact tgattacaag actcgaaaag tcgccaaact tttcttgtcc
     3301 ctcaaaaccg acgaaagtcg gttttgttgt atctggatct gttataaccg atctccttgt
     3361 tctgtcaaga gttgttcaaa aaatttgatc cacaaaacct cgacaaaacc tctccccttt
     3421 tcccgttccg ggcttcaata tcggagctga gggacaccct ggcaccaaca aaccgaccca
     3481 agac
//
