LOCUS       NZ_AP022061             4426 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella sp. WP4-W18-ESBL-05 plasmid pWP4-W18-ESBL-05_4, complete
            sequence.
ACCESSION   NZ_AP022061
VERSION     NZ_AP022061.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194464
            Sequence Read Archive: DRR199300
            Assembly: GCF_014169075.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella sp. WP4-W18-ESBL-05
  ORGANISM  Klebsiella sp. WP4-W18-ESBL-05
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Klebsiella sp. WP4-W18-ESBL-05 isolated
            from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 324x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 10:05:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,069
            CDSs (total)                      :: 4,953
            Genes (coding)                    :: 4,803
            CDSs (with protein)               :: 4,803
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 36 of 150
            Pseudo Genes (incomplete)         :: 116 of 150
            Pseudo Genes (internal stop)      :: 17 of 150
            Pseudo Genes (multiple problems)  :: 17 of 150
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022061.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Klebsiella sp. WP4-W18-ESBL-05"
                     /mol_type="genomic DNA"
                     /strain="WP4-W18-ESBL-05"
                     /isolation_source="wastewater"
                     /db_xref="taxon:2675713"
                     /plasmid="pWP4-W18-ESBL-05_4"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6514 N 139.4813 E"
                     /collection_date="2018-02-13"
     gene            101..424
                     /locus_tag="H7R58_RS25130"
                     /old_locus_tag="WP4W18E05_P40010"
     CDS             101..424
                     /locus_tag="H7R58_RS25130"
                     /old_locus_tag="WP4W18E05_P40010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_176689730.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARTGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            633..>1067
                     /locus_tag="H7R58_RS25470"
                     /pseudo=""
     CDS             633..>1067
                     /locus_tag="H7R58_RS25470"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1102..1593
                     /locus_tag="H7R58_RS25140"
                     /old_locus_tag="WP4W18E05_P40020"
     CDS             1102..1593
                     /locus_tag="H7R58_RS25140"
                     /old_locus_tag="WP4W18E05_P40020"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015060400.1"
                     /translation="MSSLLTLAKDLEQQSKAQKQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            1600..1815
                     /locus_tag="H7R58_RS25145"
                     /old_locus_tag="WP4W18E05_P40030"
     CDS             1600..1815
                     /locus_tag="H7R58_RS25145"
                     /old_locus_tag="WP4W18E05_P40030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_137500696.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSYLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2503..2718)
                     /locus_tag="H7R58_RS25150"
                     /old_locus_tag="WP4W18E05_P40050"
     CDS             complement(2503..2718)
                     /locus_tag="H7R58_RS25150"
                     /old_locus_tag="WP4W18E05_P40050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015387220.1"
                     /translation="MGFWETAGKIAKGAANALDEKAHELQATKMRLESKSSEELKRLIK
                     SDGFFGSASNTEKSMAMKILRERGDV"
                     /name="hypothetical protein"
     gene            3052..3513
                     /locus_tag="H7R58_RS25155"
     CDS             3052..3513
                     /locus_tag="H7R58_RS25155"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_128348982.1"
                     /translation="MVNTYYGYLLGVTLGEWAMGSDVQWQIDNGYLAQVMGDFSTESPS
                     FEMPMFGRYFDTFQAAIHVAKAFPGSTLQPSEYEDKTGMVIRHYYVDCHPYYDRLPPEK
                     SDDFQYDEYPSAIHVAACIDQASKELDELLFGGGYGENGEGHASPIILY"
                     /name="hypothetical protein"
     gene            complement(3769..3879)
                     /locus_tag="H7R58_RS25435"
     CDS             complement(3769..3879)
                     /locus_tag="H7R58_RS25435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063858821.1"
                     /translation="MHEPPVQSDYHAHSGNYQLESNPERHDKIAIGGSHW"
                     /name="hypothetical protein"
     Typing          4241..4414
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.714"
                     /coverage="100.0"
                     /evalue="1.2e-79"
     Typing          complement(3713..4317)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.196"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          415..1907
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.8"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gacgcgcctg cggcttgtcc aaccccgcgc cgaccgggaa agaggttccc gcccgtcccg
       61 gcgttatcag gagaccggtt tgtcagagaa acgcagcaaa atgctcacga tgtgggtgac
      121 cgaagatgag caccgccgtc tgctggaacg ctgcgacggt aaacagctcg cggcctggat
      181 gcggcagacc tgcctggacg aaaagcctgc gcgcaccgga aagctcccct cactctcgcc
      241 ggcgctgctg cgccagcttg ccggcatggg caataacctg aaccagatag cccgccgtgt
      301 taacgccggc ggcggcaccg gccatgacag ggtacagatt gtggcggcgc tgatggccat
      361 cgatgccggg cttgagcggc tgcggcacgc cgttcttgag aaaggaacgg acgatgatcg
      421 ttaaatttca tccacgcggt cgcggcggtg gcgccgggcc ggtggactat cttctgggta
      481 aagaccggca gcgtgaaggt gccagcgtcc tgcaggggaa gccggaagaa gtccgggagc
      541 tgattgacgc gtcgccttac gtgaagaaat acacctccgg cgtcctgtcg tttgcggagg
      601 ccgatctgcc tcccggccag cgggaaaaac tgatggccag cttcgagcgg gttctgatgc
      661 ccggacttga taaagatcag tacagcattc tgtgggtgga gcacgaggac aaggggcgtc
      721 tggagctgaa ctttctgatc cccaataccg agctgctgac cggcaggcgg ctccagccgt
      781 actacgaccg cgccgaccgg ccgcgtatcg atgcctggca gaccatcgtg aacggcagac
      841 tggggctgca cgacccgaac gcaccggaaa accgccgggc gctggtcacg ccgtccggac
      901 tgccgaaaac gaagcaggag gccgcagaag ccatcacgcg cggactgctg acccttgctt
      961 cgtccgggga gctgaaaacc cgtcaggacg tcactgaggc gctggaacgg tcaggttttg
     1021 aggtggtgcg caccacgaaa agcagtatca gcattgccga cccggacggg gggcgaaaca
     1081 tccgactgaa gggagccatc tatgagcagt cttttaacgc tggcgaagga cttagagcag
     1141 caatcgaaag cgcagaagca gagtaccggc gagatgctga aagccgcatt cagcgagcac
     1201 gagcagtctg tcagagcgga actgagcgca agcgcgagga gaatcagcga cgccatcctc
     1261 gcacacgagc agagtatgag cgaggcgatg gagaaaaacc ggcggagcgt actgcggact
     1321 gccgggcgga catggctcac catcctgatg gtgtcagcgc tgctgatcgg cacgagcggg
     1381 agcatactgt ggtggcaggg gcagcagata accgacaatt acacgcatct tcgtcagcag
     1441 gaagataccc tggcgaaaat gaccgcccgg acgtggggag tgcgctatca ggagagcagc
     1501 gacggccgga ggtttcttat cctgccgccg gggatgcaaa ccgaagccat cccctacgac
     1561 gggacgacat ggatccgcct gaaacaggag tagcggaaaa tgacagagct ggaaaagcag
     1621 ttgctgagcg cattagagca gctacagcag gactactcgc aaaggctgga cgaatgggag
     1681 agcgccttcg cggaatggcg gagcatgtgt gggcttatgc aacgggagaa cgcggtgctg
     1741 aacgagcgcg tgtcgtactt gagcacgcag gtgctgagtt taagcgagca gctgcgccgc
     1801 ttgtccggga actgaatgcc accgagttcc ggcagaggga gaatgagtat cagaaaacac
     1861 tggagctgga gcgccagccg cagaaaacct accgcgggcc gacactttaa accatccagg
     1921 cagctgacaa cccgcacgaa gaggggctct tacttctccc gctaaatagg ttcgtgatca
     1981 aaaaggtcac gacttattca ggagcgggaa atgaagctgt taaatcttat tgaatcattg
     2041 cttaactgcc tgtatctggc ggcggagctg tgggactggc tggagaagca tttgtcggtt
     2101 tttaaggcat tggcgcggat tttagtgtga ctttaacatc catgcatctg gctgcatgac
     2161 tatgcaccag tgtaatattc agcggctgcg tttctggtgt tttttcgtta tgtctgtcgc
     2221 cactttctcg cgtctggcac cggaaacgca gccaaccccg tttcagcggt gcgggcaatg
     2281 ggacgttatg gtaaataagt gggcgtaaaa aagcccgggt tacgggcttc tggacgctat
     2341 gacttaccac gacattctct gcaccaactt tgattcgttg caccagaagg aatggttctg
     2401 aatccgaata gagtttgcaa ctcacctgca gtatgagcgg tggttcgctt gcagttaggg
     2461 catgtatgag gaagattcac cgtgcctacc atttttctat ccttaaacgt ctccgcgttc
     2521 tctgagaatt ttcattgcca tgcttttttc tgtgttactg gctgagccga aaaaaccatc
     2581 agatttaatg aggcgcttta gttcttcact gcttttgctt tctaagcgca ttttggttgc
     2641 ctggagttca tgagcttttt catccaaagc gttagctgcg ccttttgcaa ttttaccggc
     2701 cgtttcccaa aatcccattt aaccactcct taatgcttat ccaattggac ttgttgtgat
     2761 ccgacgactc cgacatttag ggcaataatc aggcgtgcca taaccagtac gactggtcca
     2821 ctcatgctta caatccaaac acttagcccg gtagattgtc ggtgccattg aatactccat
     2881 tgattttaaa aatatttgta tagtgcacta aaattatcgg cagtttttct cggagcttga
     2941 gaggtagata accctaagct gatatgttgc ctttatgatg gttttcgtgt gccaaggtta
     3001 gctaggtaag ggatttactg tgggaagtgc tggttcgcga agtgaagcgt tatggtaaat
     3061 acatattatg gttatttatt aggagtaact ctgggagagt gggctatggg atcagatgtt
     3121 caatggcaga tagataatgg ttatttggcg caagttatgg gggatttttc gacagagtcg
     3181 cctagctttg aaatgccaat gtttgggcga tattttgata cctttcaagc tgctatacat
     3241 gttgcaaaag ctttcccagg gagtacgttg cagccatcag agtacgaaga taaaactggg
     3301 atggtaatac gtcattatta tgtggattgt catccgtatt atgatcgact tcctcctgaa
     3361 aaaagcgatg attttcagta tgatgaatac ccttctgcta ttcatgtggc tgcgtgtatt
     3421 gatcaagcca gcaaagaact cgacgaacta ttgtttggtg gtggatatgg ggaaaatggt
     3481 gaggggcatg caagcccgat aattttatat tagtgtgagg gggcggaggg atattatcca
     3541 atttgtaaaa tgatcttctt gagatcgttt tgctctgcgc gtaatctctt gctctgaaaa
     3601 cgaaaaaacc acctggggag gtggtttttc gaaggttcag taagttgggg aacttctgaa
     3661 ccgtggtaac agtcttgtgc gagacgtgtc accaaatctg tccttccagt gtagccgtag
     3721 tccggccacc acttcaagaa ctctcgatac atctctcgca catcctgctt accagtggct
     3781 accgccaatg gcgattttgt cgtgtctttc cgggttggac tcaagttgat agttaccgga
     3841 atgggcgtgg tagtcggact gaacgggggg ttcgtgcata cagtccagct tggagcgaac
     3901 tgcctacccg gaactgagtg tcaggcgtgg aatgagacaa acgcggccat aacagcggag
     3961 tgacaccggt aaaccgaaag gcaggaacag gagagcgcac gagggagccg ccagggggaa
     4021 acgcctggta tctttaagtc ctgtcgggtt tcgccaccac tgatttgagc gtcagatttc
     4081 gtgatgcttg tcaggggggc ggagcctatg gaaaaacggc ttcgccgcgg ccttctcgtt
     4141 tccctgttaa gtctcttcct cgcatctacc aggaaatttc cgccccgctc gtaagccatt
     4201 tccgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaatat
     4261 atcctgtacc acatattctg ctgacgcacc ggtgccgcct tttttctcct gccacatgaa
     4321 gcacttcact gacaccctca tccgtgccaa catagtaagc cagtatacac tccgctagcg
     4381 cttcgtgact ggttcagggc tgcgccccga aacccgccaa aagcct
//
