LOCUS       NZ_AP022029             2811 bp    DNA     circular CON 02-DEC-2023
DEFINITION  Klebsiella sp. WP3-S18-ESBL-05 plasmid pWP3-S18-ESBL-05_9, complete
            sequence.
ACCESSION   NZ_AP022029
VERSION     NZ_AP022029.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194424
            Sequence Read Archive: DRR199260
            Assembly: GCF_014168915.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella sp. WP3-S18-ESBL-05
  ORGANISM  Klebsiella sp. WP3-S18-ESBL-05
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Klebsiella sp. WP3-S18-ESBL-05 isolated
            from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 334x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/02/2023 12:00:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,520
            CDSs (total)                      :: 5,397
            Genes (coding)                    :: 5,271
            CDSs (with protein)               :: 5,271
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 38 of 126
            Pseudo Genes (incomplete)         :: 94 of 126
            Pseudo Genes (internal stop)      :: 12 of 126
            Pseudo Genes (multiple problems)  :: 16 of 126
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022029.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Klebsiella sp. WP3-S18-ESBL-05"
                     /mol_type="genomic DNA"
                     /strain="WP3-S18-ESBL-05"
                     /isolation_source="wastewater"
                     /db_xref="taxon:2675711"
                     /plasmid="pWP3-S18-ESBL-05_9"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6684 N 139.4278 E"
                     /collection_date="2018-08-06"
     gene            101..700
                     /locus_tag="H7R64_RS27500"
                     /old_locus_tag="WP3S18E05_P90010"
     CDS             101..700
                     /locus_tag="H7R64_RS27500"
                     /old_locus_tag="WP3S18E05_P90010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019704520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="WP_105568663.1"
                     /translation="MAKAKVLINDTDEATIKNVLDVDDMNEVNINVTRKKKTGFLGTYS
                     IVFHESVKTLSKMNLKPNAYKIVMYLFGIIEMGNILVNFSQKKIADDLGLQPSNVSRAF
                     NELFKKGVLIKDSEDGHVYLNSNLCTMGVPKNFDQKRMKSLQKSNIETEDFKNQINLFS
                     PTNTTKIKTNDKKVIKKKEKENVLEFPMQNDWVDIE"
                     /name="Crp/Fnr family transcriptional regulator"
     gene            944..2227
                     /locus_tag="H7R64_RS27785"
                     /old_locus_tag="WP3S18E05_P90020"
     CDS             944..2227
                     /locus_tag="H7R64_RS27785"
                     /old_locus_tag="WP3S18E05_P90020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_227675423.1"
                     /translation="MKSQFMHVELYSREEPAKKTINKSKNINHKSDVRTTTVNGVLSEM
                     KREEGFTSHLETIAAPEVLYGSIDALERSIERYEAEFKTTDKNGKEKKLRKDACILLAG
                     VVSLDRADEEIWDEYKTKAIEYLKNKYGENLKCVVEHTDETNPHFHFYVVGNRDQDLNL
                     LHDGKLAVSKLAKEDKKKLHQTAYTEAMIKFQDDFYVQVSNKFGLSRTGEEPRERYKSR
                     PDYLRFKKRQETALNILANLEEFEEKELKKAKQAGFNVGIKNFDETNFFRKLDWRVKRI
                     KKEKDNEIKKLSDENDNLNIDKSNLQHELDMSKIELSDAYSQRNEASRKNSVYIDKVNA
                     LKKENKLLLPYKDQFDLKLKQEKEPLQAEVKELTSENTNLKNENTKLKKENLDFKEFIL
                     GVKNYYGSKFQEWYEEVFNPKTKTKPKF"
                     /name="plasmid recombination protein"
ORIGIN
        1 tgaaacgata actttcttat cgttttgata ttgacattgc atttagatcg gttataaatg
       61 caacataggt tattattttt actacaaaaa ggaatataaa atggcaaaag caaaggtttt
      121 aataaacgat acagatgaag caacaataaa aaacgtatta gatgttgatg atatgaatga
      181 agtaaacatt aacgtcacga gaaaaaagaa aactggattt ttgggaactt attcaatagt
      241 ttttcatgaa tctgtaaaaa cactatctaa aatgaactta aagccaaatg cttataagat
      301 tgtaatgtac ttatttggca ttatagaaat gggtaatatt ttagttaact tttcacaaaa
      361 gaagatagct gatgatttag gcttgcagcc gtctaatgta tcacgagcat tcaatgagct
      421 atttaaaaaa ggagttttaa taaaagatag cgaagacgga cacgtttact taaattcaaa
      481 tctatgcaca atgggtgttc cgaaaaattt tgatcaaaag agaatgaaaa gtttacaaaa
      541 atcaaatatt gaaactgaag attttaaaaa tcagatcaat ttatttagtc ctacaaatac
      601 aacaaaaata aaaaccaatg ataagaaagt tatcaagaaa aaagaaaaag aaaacgtttt
      661 agagtttcca atgcaaaatg attgggttga tattgaatag taaaactaac actaccgcac
      721 ttcgtttgct gaatccctaa acctcgcaaa tataaaattt gcaagcttta gggatttgct
      781 tttttgtttt gaagttgact ttaaccccgt agggccgcat ctatggagca gcatatgaaa
      841 tatgctagcg taatagatat agtcggttcc tatagctatt gacagaaaaa caataaatgt
      901 taattacata tataaggaaa attaaaatat aggagaattt aaaatgaaat cacaatttat
      961 gcatgtagaa ctctacagca gagaagaacc agctaaaaag acaataaata aaagtaaaaa
     1021 tataaatcat aaatctgatg ttagaacaac aaccgtaaac ggtgtgttgt cagaaatgaa
     1081 aagggaggag ggttttacat cccatttaga aaccatagct gcaccggagg tgctctatgg
     1141 gtctatagat gccttagaac gctctataga gcgttacgag gccgaattta agactacaga
     1201 caagaacggg aaggagaaga agctacgcaa agatgcatgc atcttgctag ctggtgtggt
     1261 ttctctagat cgagctgatg aagaaatttg ggatgaatat aagaccaaag caattgaata
     1321 tttaaaaaat aaatatggtg aaaatttaaa atgtgttgtt gagcatacag acgaaaccaa
     1381 cccacatttt catttttacg tcgttggaaa cagggatcaa gatttaaatt tgcttcatga
     1441 tggaaagctc gcagtatcta aattagcaaa agaagataag aaaaaattac atcaaacagc
     1501 ttacacagaa gctatgatca aatttcaaga tgatttttat gtacaggtat caaataaatt
     1561 tggattgagc agaacgggcg aggagccgcg agaacgctat aaatcacgcc ctgattactt
     1621 gagatttaaa aaaagacaag aaacagcatt aaatatatta gccaatttag aagagtttga
     1681 agaaaaagaa ttaaaaaaag caaaacaagc tggctttaat gttggtatta aaaatttcga
     1741 tgaaactaat ttttttagga aactcgattg gagagtaaaa agaataaaaa aagaaaaaga
     1801 caatgagatt aaaaaattat cggatgaaaa cgataattta aatatagata agtcaaattt
     1861 acagcatgaa ttagatatga gtaaaattga gctttcagat gcatatagtc aaaggaatga
     1921 agcatccaga aaaaatagcg tttatattga taaagtaaac gctctaaaaa aagaaaataa
     1981 attattactt ccatacaaag atcaatttga tttgaaatta aagcaggaaa aagagccatt
     2041 gcaagcagaa gtcaaagaat tgacttctga aaataccaat ttgaaaaatg agaacacaaa
     2101 attaaaaaaa gaaaatttag atttcaaaga atttattttg ggtgttaaaa attactatgg
     2161 atctaaattt caggaatggt acgaggaagt attcaaccca aaaacaaaaa caaaacccaa
     2221 attttaattg aatattttca tatttgccgc ctatgtggtg tatgagacac caccagaagc
     2281 ggcctgacag cgtttagctg tcatagctgg gatttatccc aaaaaaagtg gttttgcacg
     2341 cagttagtta cctttaaata tcagtcactt aaatacaaaa aatgcaggtt gggaaaatgt
     2401 ggtcagggag ctggccgcat tggggtgggg gtaccttcat aacgccttgg tgcgttgtga
     2461 agagggggaa cggggcgggg ttcccccttt ggtatgtaac tacatataaa acaaagaaat
     2521 actgttaaac cttgaaccgt gagaactgca gaaccacttg tggttctgtg gttcgagggg
     2581 ttggcctgcc cggcgagggc ggttttgcag ttgattttac ggtttcttct ttcttatttt
     2641 cttacttctt aagcaccctt taaagcctta tatatcaaca ctttaccaaa aaaaaaatta
     2701 tgaaaatcat aattttcgat tatgaaaatc ataatgcaaa attatgaaaa tcataatcag
     2761 acttatgaaa atcataattt tgcagacagg tgcattgata tgatttgatg t
//
