LOCUS       NZ_AP022028             3139 bp    DNA     circular CON 02-DEC-2023
DEFINITION  Klebsiella sp. WP3-S18-ESBL-05 plasmid pWP3-S18-ESBL-05_8, complete
            sequence.
ACCESSION   NZ_AP022028
VERSION     NZ_AP022028.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194424
            Sequence Read Archive: DRR199260
            Assembly: GCF_014168915.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella sp. WP3-S18-ESBL-05
  ORGANISM  Klebsiella sp. WP3-S18-ESBL-05
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Klebsiella sp. WP3-S18-ESBL-05 isolated
            from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 334x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/02/2023 12:00:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,520
            CDSs (total)                      :: 5,397
            Genes (coding)                    :: 5,271
            CDSs (with protein)               :: 5,271
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 38 of 126
            Pseudo Genes (incomplete)         :: 94 of 126
            Pseudo Genes (internal stop)      :: 12 of 126
            Pseudo Genes (multiple problems)  :: 16 of 126
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022028.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Klebsiella sp. WP3-S18-ESBL-05"
                     /mol_type="genomic DNA"
                     /strain="WP3-S18-ESBL-05"
                     /isolation_source="wastewater"
                     /db_xref="taxon:2675711"
                     /plasmid="pWP3-S18-ESBL-05_8"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6684 N 139.4278 E"
                     /collection_date="2018-08-06"
     gene            complement(101..292)
                     /locus_tag="H7R64_RS27485"
                     /old_locus_tag="WP3S18E05_P80010"
     CDS             complement(101..292)
                     /locus_tag="H7R64_RS27485"
                     /old_locus_tag="WP3S18E05_P80010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_023063538.1"
                     /translation="MSEKQRREGFRKAEASLRLEGLDPSGTPHYELVKARIISGEISYE
                     QGRKEIFDYYVQKARSKE"
                     /name="antitoxin VbhA family protein"
     gene            complement(289..1257)
                     /gene="mobV"
                     /locus_tag="H7R64_RS27490"
                     /old_locus_tag="WP3S18E05_P80020"
     CDS             complement(289..1257)
                     /gene="mobV"
                     /locus_tag="H7R64_RS27490"
                     /old_locus_tag="WP3S18E05_P80020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852935.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_182928890.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNANAERTPENYCS
                     VSESTDQAMGRVRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFARSEQWLE
                     KKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDSQQKRLQSVTEPFKGLSREQM
                     THILTMAQTFQQQNHDREKQRQLEREQERQQRQKTDRGISR"
                     /name="mobV"
     gene            2281..2832
                     /locus_tag="H7R64_RS27495"
                     /old_locus_tag="WP3S18E05_P80040"
     CDS             2281..2832
                     /locus_tag="H7R64_RS27495"
                     /old_locus_tag="WP3S18E05_P80040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_182928889.1"
                     /translation="MSLVRYKTNPFLDDMIVPVKGQKVQISKLGKDNNILVNQSTGEVH
                     GTHVTTYRKVDSEQFVKLFTANIALTFQLSSAGIKAFGVLLWVVQNKALSKDEVDLDSY
                     ALDEFYELHKDSEPPLRLSHPTFKRGINELEKAQIVAKTLRKGRYFINPNFVFNGDRVA
                     FTTVIERKKAESPEIKEISS"
                     /name="replication/maintenance protein RepL"
     Typing          complement(395..1266)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.845"
                     /coverage="86.0"
                     /evalue="0.0"
ORIGIN
        1 tattataata aacataaaaa gcaaatatat tttatataaa atggaaaata tattttcacg
       61 cttttaaata aatataaata ttgtgtctta atatgagttc ttattctttg gagcgagctt
      121 tctgaacgta ataatcaaaa atctcctttc gtccctgttc atagctgatt tcacccgaaa
      181 taatgcgcgc tttaactaac tcatagtgag gcgttccgct gggatccagc ccttcgagcc
      241 ttaatgaggc ttccgctttg cgaaaacctt cacgtcgctg tttttcgctc atcggctaat
      301 acctctgtca gttttctgac gttgctggcg ttcctgctcc cgttcaagct ggcgctgttt
      361 ttcacgatcg tgattctgtt gctggaacgt ctgcgccatc gtcagaatat gggtcatctg
      421 ctcccgggaa agtcccttaa aaggctctgt aacgctctgt aggcgttttt gctgcgaatc
      481 catagtcttc tgtagctccc gcgctctttt tgcattctga gcgctctgag aggccatagc
      541 gatggttcct gcaaagccat cgttcactgc gtggcttaga cgtttggcta tggctgcctg
      601 atcttccacg tctttggtga aaatgccctt ccgcaataca cgcggctcca gcgcctccgg
      661 cgagatcgat acctggctac gggtgccacg gtttatcgat tcgtagtagt gctgaactgt
      721 ctgatgcgtg gcacggcttc cctcgatgcc gcgctcaaga cccagttttt ttactgattc
      781 agcgtaggtg ctctgatctt cacgcatctt tgaccgcccg ccaataaact ctttcgcgga
      841 cagccgtcca tcctgcgtca atggcaccac gaacgctgac aagtgcgggg tggcctcgtc
      901 ccggtgaacc accgccgcca caacacgatc cttcccgtat ttcttctcca gccattgctc
      961 agagcgggca aaaaactccg cctgttgccg cggtgtcgct tctttccacc actcagggct
     1021 tgcagtcata acgtattcaa cggccagtac agcatcttta cggcgttttt caggcagcag
     1081 ctcccggaca cgccccattg cctgatccgt tgattcggat acagagcagt agttttctgg
     1141 tgtccgttca gcgttagcat tcggcgtctc acgctccctg tagcagtgct gcaaagcgct
     1201 ggccacactg cccattcccg tcagcttttt acagcgcatt atcgcgtagt tcgccatgct
     1261 tccccccctc gttccggcgt ccatccggcc agacggggga tcgcattgcg atgcctccgg
     1321 cgggccacta tctattgata gtgtactcac tagactttgc ttcgcaaaat cgttcggtct
     1381 ggctcacgcc agacaggaaa aacaacatca accgccaggg aacaacagcg gtaaagtgtc
     1441 gttgataaat ctcagcagct cgatgctgaa attgaacaat tgccagacaa tttccaaaac
     1501 atgtgtgtat ctcatttagt ctttctccgt attggttttt gtaccggccg tcgcttcaca
     1561 acatccggct atgacaagaa gataatcggt ctgtgctttg gcccgaaacg gtagccgttg
     1621 tatcgcaggc catacaaaac aaaccttcct gcagacgccc gaacagggtc gcatcacgac
     1681 tttgaggtat ggcgaacttt ttgtattcgt gtgggttgtc agggatcacg acaacatcga
     1741 ggtcaagggc aaagtcgttt tttacctggt gtccgataaa aaatcttctt ggggtttggc
     1801 agaaaaagcg tctggcaggt ggatggcctc actgcgtgaa accatccacc cctgataagc
     1861 atttgtttta cggtgagtca tcaatggtta aatcggaacc caccgcttgc ggcgggaccc
     1921 cgcgatttaa tcattgacga tccagctaat ggaattggtt ttgagagagc aaggaccgct
     1981 cgccgcaaga tcggattagg gagcggcaac gccgcgagtc tgtccgattg aggaagcgga
     2041 atatcacgga aactacctca ggcactttcg ctcaggacct ctgttttttc ttcttagttc
     2101 ttagcgatag ataagaacta agaagggact actcgcaaac ccgcatgtag caaggatcgt
     2161 attttcaaaa tcagtacctt caaggctcaa tctacgtact ttgtaagctc aaaatagttg
     2221 ttgattttga gcttacggct atctaaaatc gaattgatgg tatcaattta ggggtagata
     2281 atgagcttag taagatacaa aactaaccca tttttggatg atatgattgt tccagtaaag
     2341 ggacagaaag tgcagatctc taagctggga aaagacaaca atatcttggt taaccaatcg
     2401 acaggtgagg tacacggaac gcacgtaact acatatcgaa aggttgattc agagcagttc
     2461 gtaaagcttt tcacagccaa tattgcttta actttccagt tgtcatcagc aggtataaaa
     2521 gcattcgggg ttttattatg ggttgttcaa aacaaagctc tctcaaaaga tgaggtcgat
     2581 ttagattcat atgccttaga tgagttttat gaattacata aggactcaga acccccactg
     2641 cgactaagcc accctacttt taaacggggg ataaacgaat tagaaaaagc ccagattgtt
     2701 gcaaaaacac ttcggaaagg tcgctatttt attaacccaa actttgtatt taatggcgat
     2761 agagttgctt tcacaactgt cattgaaaga aaaaaagcag agtccccaga aataaaagag
     2821 atttcgtctt gataagactc acagaactca accaaggact acctatcaag catccgcagc
     2881 atgcctaccc tgtgtaacta atggaaaacg gatgcatcca aaataaaaaa gcagctaaaa
     2941 gctgcttttt actttcacca acaaacagac caaggataca tagaaataat aacaatcgtt
     3001 atttttatta ttctcatacg cttggctact ttttcttttt tattcatgcg tagcctcata
     3061 ttttattatt tatattgacg acattactcg ccgttcttct tacttaaaaa tataaataaa
     3121 aagtaacaga aactttata
//
