LOCUS       NZ_AP022027             4028 bp    DNA     circular CON 02-DEC-2023
DEFINITION  Klebsiella sp. WP3-S18-ESBL-05 plasmid pWP3-S18-ESBL-05_7, complete
            sequence.
ACCESSION   NZ_AP022027
VERSION     NZ_AP022027.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194424
            Sequence Read Archive: DRR199260
            Assembly: GCF_014168915.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella sp. WP3-S18-ESBL-05
  ORGANISM  Klebsiella sp. WP3-S18-ESBL-05
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Klebsiella sp. WP3-S18-ESBL-05 isolated
            from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 334x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/02/2023 12:00:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,520
            CDSs (total)                      :: 5,397
            Genes (coding)                    :: 5,271
            CDSs (with protein)               :: 5,271
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 38 of 126
            Pseudo Genes (incomplete)         :: 94 of 126
            Pseudo Genes (internal stop)      :: 12 of 126
            Pseudo Genes (multiple problems)  :: 16 of 126
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022027.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Klebsiella sp. WP3-S18-ESBL-05"
                     /mol_type="genomic DNA"
                     /strain="WP3-S18-ESBL-05"
                     /isolation_source="wastewater"
                     /db_xref="taxon:2675711"
                     /plasmid="pWP3-S18-ESBL-05_7"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6684 N 139.4278 E"
                     /collection_date="2018-08-06"
     gene            complement(101..487)
                     /locus_tag="H7R64_RS27470"
                     /old_locus_tag="WP3S18E05_P70010"
     CDS             complement(101..487)
                     /locus_tag="H7R64_RS27470"
                     /old_locus_tag="WP3S18E05_P70010"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045282373.1"
                     /translation="MRISNVEWLKKRIAFIRKLGEKTARQRQIIDLLDDEASLSETERR
                     LLHVLATAEKNALQERDAAQKKAVQARISGQKNRRERNHRLFLAAGLMIDAGLVDTKTG
                     ELNFDHEKLLTALKRVRYDLETSA"
                     /name="hypothetical protein"
     gene            654..1589
                     /locus_tag="H7R64_RS27475"
                     /old_locus_tag="WP3S18E05_P70020"
     CDS             654..1589
                     /locus_tag="H7R64_RS27475"
                     /old_locus_tag="WP3S18E05_P70020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_045626452.1"
                     /translation="MATYHLSVKFGGKGKALAHASYITREDKFSQRQDLEHTEHGNMPE
                     WARDEPAHFWQAADAFERANGSTYREIEIALPRELNEAQRLALVRDFVKQETGDKHAWT
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERSPEQYFRRYNAKFPARGGARKDSGHNL
                     TPTEQKQALKALRERWERTHNAHMKRYGFEQGFIDSRSLKTQGIDRKPEQHLGFEAAGR
                     LDDSQRQDIKDSRTFSVVDVPEQAKHIDHSDRSWMNASGIEVNRKELVRSLTHDISQQY
                     GREMAARVTKQGNVEIERVKSLSRGFSISR"
                     /name="MobA/MobL family protein"
     gene            1592..2122
                     /locus_tag="H7R64_RS27480"
                     /old_locus_tag="WP3S18E05_P70030"
     CDS             1592..2122
                     /locus_tag="H7R64_RS27480"
                     /old_locus_tag="WP3S18E05_P70030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012634455.1"
                     /translation="MASTEEKLLTLLLSAEHLKEAAKEQQKAVADSLAVARETLKGYRE
                     ISDSVAQQVRDEVRQEVQKMDVAGLIGERISADFQRLEKSVGNMKINADALDKEISIIR
                     SNIIAEYNTLRGYSWKWFAGVAVAFVVLIFATSWFVKTELQDNYNASAAVYQKVADLEK
                     LLTTEQKPEKKKN"
                     /name="hypothetical protein"
     Typing          655..1586
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.177"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cctatccgaa aatccctacc gctcgccgca gccgaacgac cgagcgacgc gagtcagtga
       61 gcgaggaagc ggcatttctc cggccttgca gaatgcacgt tcacgccgat gtttccagat
      121 cgtagcgtac ccgtttcagc gcagtcagta atttttcgtg gtcaaaattc aactcacccg
      181 tttttgtatc aaccagtccc gcgtcaatca tcagaccagc cgccagaaac agtctgtgat
      241 tgcgttccct gcggtttttc tgccctgaaa tccgcgcctg aaccgccttt ttctgtgcgg
      301 catcccgttc ctgcaacgcg tttttttcag cggttgccag aacgtggagc agtcgtcttt
      361 cagtctcact gagcgaagct tcatcatcca gcagatcgat aatctgccgt tgccgtgccg
      421 ttttttctcc cagctttctg atgaaggcta tgcgcttttt aagccattcg acgttactga
      481 tccgcatcgt ttaacttccc catcccgata tttccgacgg ggaaagtata taacttgcgg
      541 tcagaagtcg catgacttat ctttactcat aattctttaa attagagtaa agcgcactta
      601 tatgtttctc cctgcgggca gaaacagtgc gccctgccgg gggctgacac aacatggcaa
      661 cgtatcatct cagcgtgaaa tttggcggca agggtaaagc ccttgctcat gccagttaca
      721 ttacgcggga agataagttc agccagcgcc aggatctcga gcacaccgaa cacggcaata
      781 tgccggaatg ggcgagagat gaacccgccc atttctggca ggctgcggat gcgttcgaac
      841 gtgccaacgg ttcaacgtac agggaaattg agatcgctct accccgcgag ctgaatgaag
      901 cgcagcggct ggcgcttgtc cgggattttg tgaagcagga aacgggcgac aaacatgcat
      961 ggacgtttgc gatccacaat ccgaaagcca gcattgacgg cggcgaacag ccgcacgccc
     1021 atatcatgat gagccagcgc gtcaatgacg gtattgagcg cagtcccgaa cagtatttca
     1081 ggcgctataa cgccaaattt ccggcgcgtg gcggcgcgcg caaggactca gggcataatt
     1141 taacccctac agaacaaaaa caggcactga aagccctgcg ggagcgctgg gagcgtacgc
     1201 acaacgcgca tatgaaacga tatggctttg agcagggttt tattgatagc cggtcactga
     1261 aaacacaggg tatcgaccgg aaaccggaac agcatctcgg ttttgaagca gccggacgac
     1321 tggacgacag ccagaggcag gatattaaag acagccggac gttttcagtc gtggatgtgc
     1381 ctgaacaggc gaaacatatc gaccacagcg accgtagctg gatgaatgcc agcggtatcg
     1441 aggtaaaccg gaaggaactg gtcagatctc tgacgcatga tatctcgcag cagtacgggc
     1501 gggagatggc tgcacgggtc acaaaacagg gaaatgttga aatagagcgc gtgaaatccc
     1561 tgtcacgcgg attttccata agcaggtaac tatggcgagc accgaagaaa aacttctcac
     1621 actcctgctg tcggcggagc atctgaaaga ggccgcaaaa gagcagcaga aagccgtggc
     1681 ggattcgctg gcggtggcaa gggaaaccct gaaaggctac cgggaaatat cggacagtgt
     1741 ggcacagcag gtaagggatg aagtccggca ggaagtgcag aaaatggatg ttgcggggct
     1801 gattggcgag cgtatcagcg ctgattttca gcgtctggaa aaatccgtcg ggaacatgaa
     1861 aatcaatgcg gacgcgctgg acaaggaaat ttccattatc cgcagtaaca tcatcgcgga
     1921 gtacaacacg ctgcggggtt acagctggaa gtggtttgct ggggttgcgg ttgcctttgt
     1981 cgtactgata tttgccacaa gctggtttgt gaaaacggaa ctacaggaca attacaacgc
     2041 gtctgctgcc gtctatcaga aagtggcaga ccttgaaaag ctgctgacaa cggaacagaa
     2101 accggaaaag aaaaagaact gacgcgctgc gcttgtccaa cgcctcacgt tcgcacaata
     2161 aattgcgcac ccgttcaccg ttttcagccg agtttggggc gtcttttgag acttccggcc
     2221 tgaattcagg ccaaaatcgc ccgcgagtca gtccggtgca tagtcatgca tagggctgaa
     2281 aatctctcag cgcgtttctg gcgggttttc gggtggtttg ttgcctgttt taccggtttc
     2341 ccgtcagaaa cgccctgaga gcctctcagg cggtgcgggc aacggatgtt atggtaaatc
     2401 cggtggcttt gacgtgcctg ccgttgtgta ccgtcgtgag tgaaaataat tatattgttc
     2461 gaagaacagc gctttttggc cggagaaaag cagttctgca aacgatggta tatcgatatg
     2521 agttcttttg gcattaatcc aggcaagacg ccgggcaata agcgtaatat tccggatgtt
     2581 acttatttcc atttctgtca acagcatccc cccccacgcc ggagaataaa aaagccgcct
     2641 gttccatcgg caagccctac atcaagggaa actacgtctt acgattcgtt ttgaaccggg
     2701 acgctttgtt gctgcgaatc actaaccaga taatccacgt tttgtgagtg ggtttaggtt
     2761 aaggcttcaa tagaacaata atagcgttaa gtgattaggg caacctgtga ttgctaaaat
     2821 gatgaagtct tcacatcatt tcggtgaagg cgatatgtgt tacaccacgt attgagagac
     2881 ctgcatgccc tttacaggtg aacgggaacg ggaatagagc ggcgatttag ccagagatgg
     2941 gtttcataac actgtaattc atatcacaat tattataact ggcggtattt ttttaacctt
     3001 tgcaattcgt tttatctgat gtttatgata attttcaaaa agttggcccc ctgacatcac
     3061 cgggagccaa aaaaaataca ccagttatta ttgtggctat attatttcat gagtaaacaa
     3121 atatgtcagg cgtatatttg tttactcaaa ttgcttgttt tttagtcatc gtgatgatga
     3181 gcgagagaaa atcgatgttg ataggttttt tatgcttttg atttttttat atttttgtat
     3241 tatttaaact gtttttctgt attgtgtagc attcccttta gatttttatg ccatcgtcgc
     3301 cgatggcttt tttattccgt taaggtaatt tttgagttca tttgcatgaa cgactgatgt
     3361 tctccatgtt cgttttcact attctttttc tcccgcgaat ttgatttatg tggttgtgac
     3421 attagttatg agatagagcc accagtgtga tgaactcaca ctacaaattc gttcagcact
     3481 aaatcagaat tcgatgcaaa tctcggacgc tggatcatca cagtgaatcc ttctataacc
     3541 cggatttgag agtcgcaaac ccatattagg gcaaagaaag cctaatgccc ccgtggtggg
     3601 tacaccagaa aacggggagc gaagcgacca gcatcaggcc gttcgtatgc tgcttcgatt
     3661 ttgtgggtgt tgcgccagac cttggcaatc acgcttcacc gcaaccgctg ccttcactct
     3721 cactttatca ggtacgttat tggttaacgt gagaccgggt agagccagct tctttcccga
     3781 gctggtcagg ttcccaccgc ttgcctgttt atcctgccgg agtccggggc gtagacctga
     3841 gagtcaaggt gcagatatgg gtttaatgtc gttgaatggt attaagctgg tagtgacatt
     3901 cctagccagc cctgctagga tttgattgtg acgtctgatc ctggcaggta tctgcaagaa
     3961 ttagagtact caagccccgc gattctgtca cggggctttt gtacatctgg agcaagaaaa
     4021 ttcatgct
//
