LOCUS       NZ_AP022026             4616 bp    DNA     circular CON 02-DEC-2023
DEFINITION  Klebsiella sp. WP3-S18-ESBL-05 plasmid pWP3-S18-ESBL-05_6, complete
            sequence.
ACCESSION   NZ_AP022026
VERSION     NZ_AP022026.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194424
            Sequence Read Archive: DRR199260
            Assembly: GCF_014168915.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella sp. WP3-S18-ESBL-05
  ORGANISM  Klebsiella sp. WP3-S18-ESBL-05
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Klebsiella sp. WP3-S18-ESBL-05 isolated
            from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4616)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 334x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/02/2023 12:00:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,520
            CDSs (total)                      :: 5,397
            Genes (coding)                    :: 5,271
            CDSs (with protein)               :: 5,271
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 38 of 126
            Pseudo Genes (incomplete)         :: 94 of 126
            Pseudo Genes (internal stop)      :: 12 of 126
            Pseudo Genes (multiple problems)  :: 16 of 126
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022026.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Klebsiella sp. WP3-S18-ESBL-05"
                     /mol_type="genomic DNA"
                     /strain="WP3-S18-ESBL-05"
                     /isolation_source="wastewater"
                     /db_xref="taxon:2675711"
                     /plasmid="pWP3-S18-ESBL-05_6"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6684 N 139.4278 E"
                     /collection_date="2018-08-06"
     gene            complement(101..940)
                     /locus_tag="H7R64_RS27435"
                     /old_locus_tag="WP3S18E05_P60010"
     CDS             complement(101..940)
                     /locus_tag="H7R64_RS27435"
                     /old_locus_tag="WP3S18E05_P60010"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_182945348.1"
                     /translation="MINFIFISKDDRLLSFAGSEPSLPINESLISNGEKGVISKVKIKN
                     NTKHSDLMGCVLSELGDFYSAYSGVVLFCDSDYYPYLCKHAGLFFMVFDLSSCSADLAD
                     GQLKQKIESLMSLSVKKFMQIKRELLISNGILMRLPYRNFINPLLRGFFDSLKRVHEID
                     IGNLAADIKKIKNDCYKKVPDVNPGRIFVDVNLNNFVFGHEYHSKQGTSSANGHVLLCD
                     VSSKYRFSHRIDEFRHFNVQKRGKKPISGSFYNCHDTRESINKETHINMFTSDFMEY"
                     /name="hypothetical protein"
     gene            complement(947..1342)
                     /locus_tag="H7R64_RS27440"
                     /old_locus_tag="WP3S18E05_P60020"
     CDS             complement(947..1342)
                     /locus_tag="H7R64_RS27440"
                     /old_locus_tag="WP3S18E05_P60020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_182928887.1"
                     /translation="MSDIKKKLSVEVRNYWNKKHKPLLFSTIGDKFKEIKSEEGFVNIG
                     GWISENINDLDAFIYSDPNKPEYIGLVPNGEEYEPEEPKLFSTKISKKSQNNERLVLDF
                     LSILNDLSDDDLKRVIIPTDILVRLMK"
                     /name="hypothetical protein"
     gene            complement(1678..1893)
                     /locus_tag="H7R64_RS27445"
                     /old_locus_tag="WP3S18E05_P60030"
     CDS             complement(1678..1893)
                     /locus_tag="H7R64_RS27445"
                     /old_locus_tag="WP3S18E05_P60030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137421.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSQG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1897..2382)
                     /locus_tag="H7R64_RS27450"
                     /old_locus_tag="WP3S18E05_P60040"
     CDS             complement(1897..2382)
                     /locus_tag="H7R64_RS27450"
                     /old_locus_tag="WP3S18E05_P60040"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_023139312.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLTASGASILWWQGQQIL
                     DNYTTIREQKRTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<2417..3121)
                     /locus_tag="H7R64_RS27895"
                     /pseudo=""
     CDS             complement(<2417..3121)
                     /locus_tag="H7R64_RS27895"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3060..3383)
                     /locus_tag="H7R64_RS27460"
                     /old_locus_tag="WP3S18E05_P60050"
     CDS             complement(3060..3383)
                     /locus_tag="H7R64_RS27460"
                     /old_locus_tag="WP3S18E05_P60050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_181421366.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSL
                     SPALLRQLAGMGNNLNQIARQVNGGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKEAG
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(3592..3795)
                     /locus_tag="H7R64_RS27465"
                     /old_locus_tag="WP3S18E05_P60060"
     CDS             complement(3592..3795)
                     /locus_tag="H7R64_RS27465"
                     /old_locus_tag="WP3S18E05_P60060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_227675422.1"
                     /translation="MEKRQRRILFPLFLISTQSLYPAIREPVTPAAVSRQERSDRVSEE
                     ASYHPYLHIALTPHSHIFLLLH"
                     /name="hypothetical protein"
     gene            4024..4107
                     /locus_tag="H7R64_RS27900"
     CDS             4024..4107
                     /locus_tag="H7R64_RS27900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058655973.1"
                     /translation="MHEPPVQSDRCALSGNYRLESNPERHD"
                     /name="hypothetical protein"
     Typing          complement(3493..3672)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="89.56"
                     /coverage="83.0"
                     /evalue="7.43e-58"
     Typing          3652..4133
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.123"
                     /coverage="93.0"
                     /evalue="2.1e-176"
     Typing          complement(1575..3070)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.984"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tctttgtgct atagtaagac gttcatttta agtactgacc aaaagtaata gggtattttt
       61 acccaaaaaa aggccaagag aaatcttggc cttttttact tcaatactcc atgaaatcac
      121 tcgtaaacat gttaatatga gtctccttat ttatactttc ccttgtgtca tggcagttat
      181 aaaatgatcc agatatgggt ttcttacccc ttttctgcac attaaaatgc ctaaactcat
      241 cgatcctatg actaaaccta tacttagagc ttacatcgca aagtaaaaca tgcccatttg
      301 cagacgacgt accttgcttt gaatgatatt cgtgaccaaa aacgaaatta ttcaagttta
      361 catctacaaa tattctaccg ggatttacat ctggcacttt tttatagcaa tcgtttttta
      421 ttttctttat atcagcagca agattaccta tatcaatctc atgaaccctc tttagagaat
      481 caaagaatcc ccttagcaac ggattgataa aattacgata tggcagcctc atcaaaatac
      541 cattactaat aagaagctct cttttaatct gcatgaattt cttcactgac aaactcatta
      601 aagattcaat tttttgtttt agctgcccgt cagctagatc tgctgaacaa ctactaagat
      661 caaaaaccat gaaaaaaagc ccagcatgct tacaaaggta agggtagtag tctgagtcac
      721 agaacagcac cactccgctg taagctgagt aaaaatcacc caactcagat agtacacacc
      781 ccattagatc actatgtttc gtattatttt ttatttttac tttcgagata actccttttt
      841 ctccgtttga aatcaaagat tcatttatag gcaatgatgg ttcactaccg gcaaatgaca
      901 aaagccggtc atcctttgaa ataaaaataa aattaatcat gagcgattac ttcataagtc
      961 taaccaaaat atccgttggg atgatcactc gcttcaaatc atcatcactt agatcgttaa
     1021 ggattgaaag aaaatccaaa accagccttt cattattctg acttttctta cttatctttg
     1081 tagaaaatag tttaggctct tccggctcgt actcttcccc attaggaact aaaccgatgt
     1141 attctggttt gttggggtca ctataaataa atgcgtcaag atcatttatg ttttcagaaa
     1201 tccaaccacc tatgttaaca aaaccttctt ctgactttat ttctttgaac ttatcgccaa
     1261 ttgtactaaa cagcaaaggt ttgtgttttt tattccaata atttctaact tctacactta
     1321 acttcttctt aatatcactc ataagtcatc cttaaataaa attcatttac cataacatct
     1381 cttggctgca cctgccgatc cgggttgggt gcgtttccgg cgcgaaacgg gcataagtgg
     1441 cgacagacac accggaaaga cgccggaaac gcacgggata aatttacccg ttttgcatag
     1501 ccatgcaaca caatgattac acgcaggttt ggtgctgatt gggtgctata agcccgattt
     1561 tcaccagtta tcacagcgac gggccgtcat aagtacgctg gcgctgcgat cgttccagct
     1621 ccagcgattt ctggtgctgc accgcgcgca cctgctcccg gtgcgcctcg ataccgttca
     1681 gccttgcgac aaccggcgca gctgctcgct caaactctgc acctgcctgc tcaagcccgt
     1741 gacgcgctcg ctcagcgccg cgttctcccg ttgcataaga ccagacatcg tccgccattc
     1801 cgcgaaggcg ttctcccact cgtccagcct tttcgagtag tcctgctgta gctgctctaa
     1861 tgcgctcagc aactgttttt ccagctccgt catgtgctat ttccccgcca gtatcatcca
     1921 gctcgaatcc tctccgaacc gtcccgcttc cgggttcacc ctcacgcagc ggcgtctctg
     1981 ctctccgcag gtcgagagct gcacgccgct gttcctctcg gacagcatgg cctgcgtgcg
     2041 cttctgctcc cggatggtcg tgtaattgtc gagtatctgc tgcccctgcc accacagaat
     2101 gcttgcgccc gatgctgtca gcagtgcaga caccaggacg atggtcagcc acgtctggct
     2161 gaccatacgc accatgcctt tcgtgcgctg gctcatggct gaggacagct tccggtcgtg
     2221 gtcgaggatg gcggcgctga ttctcttctc gctttcgctc agttccgctc tgacagactt
     2281 ctcgtgctcg ctgaatgcgg ctttcagcat ctcgccggta ctctgctgct gcgctttcga
     2341 tttctgctct aagtccttcg ccagcgttaa aagactgttc atagatggct cccttcagtc
     2401 ggatgtttcg ccccccgtcc gggtcggcaa tgctgatgct gcttttcgtg gtgcgcacca
     2461 cctcaaaacc tgcgctttcc agcgcctcag tgacgtcctg acgggttttc agctccccgg
     2521 acgaggctaa ggccagtaaa ccccgcgtaa tcgcctgagc ggcttcctgc ttcgtttccg
     2581 gcagcgcgga cggcgtgacc agggcccgcc ggttttccgg cgcgttcggg tcatgcagcc
     2641 ccagtctgcc gttcactatg gtctgccagg catcgatgcg cggacggtcg gcgcggtcgt
     2701 aatacggctg gagacgcctg ccggtcagca gctccgtgtt cgggatcagg aagttcagct
     2761 ccagccgccc cttgtcctgg tgctcaaccc aaagcacgct gtactggtct ttatcgagtc
     2821 cgggcatcag aacccgctcg aagctcgcca tcagcttttc acgctggccg ggcggtaagt
     2881 cctcttcggc aaaggacagg acgccggagg tgtatttttt ggcgtagggc gaggcgtcga
     2941 taagctcccg cacctcttcc ggcttccctt gcaggacagt ggcgccttca cgctgccggt
     3001 ctttgcccag cagataatcc accggaccgg cgccgccgcc gcgcccacgc gggtgaaact
     3061 taacgatcat cgcctgcctc cttttccagt acggcatgcc gcaatcgctc gagtccggca
     3121 tcgatggcca tcagcgcggc gaccacctgc acgcggtcgt gcccgctgcc accaccgccg
     3181 ttaacctgcc gggcaatctg gttaaggttg ttccccatgc cggcaagctg acgaagcagc
     3241 gccggcgaga gcgacggaag tttgccggcg cgggcaggct tctcgtccag acacgtctgc
     3301 cgcatccagg ctgcgagctg cttaccgtcg cagcgttcca gcagacgccg gtgctcatct
     3361 tccgttaccc acatcgtgag cattttgctg cgtttgtctg ccaatccggc ctcctgataa
     3421 cgccgggacg ggcgggaaat cttttcccgg tcggcgcggt gttggacaag ccgcaggcgc
     3481 gtcagcggct tttagcgggt ttcggggcgc agccctgaac cagtcacgta gcgctagcgg
     3541 agtgtatact ggcttaatat gttagatcgg agtggtaact caggaaagtg cttaatgtag
     3601 caggagaaaa atgtggctat ggggcgtaag tgcaatatgc aggtaaggat gatatgacgc
     3661 ctcctcgctc actcggtcgc tgcgctcctg ccgtgagact gcggcgggcg ttaccggctc
     3721 acgaatggcg ggataaagag actgagtgga aatcaggaag agcgggaaaa ggatgcggcg
     3781 ttgccgtttt tccataggct ccgcccccct gacaagcatc acgaaatctg acgctcaaat
     3841 cagtggcggc gaaacccgac aggactataa agatcccagg cgtttccccc tggtagctcc
     3901 ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg tcattccgct gttatggccg
     3961 cgttttctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
     4021 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tgtccggtaa ctatcgtctt
     4081 gagtccaacc cggaaagaca cgactaatcg ccattggcaa cggccactgg taaacaggat
     4141 gtgcgagaga tgtatcgaga gttcttgaag tagtggcctg tctgaggcta cactggaagg
     4201 acagatttgg tgacatgtct cgcacaagac tgttaccacg gttcgaagag ttggtagctc
     4261 taagaacctt gaaaaaccac cgttggtagc ggtggttttt tcttttacag aatcagatac
     4321 tacgtctaac gctagatagc ggtcaatacg cgcaggattg gtcgcagcct aaaaagcatg
     4381 gtattatcac ttcactaaag atttatgttt ggcaagtaca agaactctcc gccttgtatt
     4441 ttgaaggtag gatgttcacg aggtgacgga ttttgtctgg caatttagtt agccgaaaga
     4501 agcacctgaa gactgatgtg tagtcagctc acaacattcg ttaacacttg tgttgtgctg
     4561 agggcaagca tccataagtc gatgataaga tcgagctgta gcattttgag aaaata
//
