LOCUS       NZ_AP022023             9350 bp    DNA     circular CON 02-DEC-2023
DEFINITION  Klebsiella sp. WP3-S18-ESBL-05 plasmid pWP3-S18-ESBL-05_3, complete
            sequence.
ACCESSION   NZ_AP022023
VERSION     NZ_AP022023.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194424
            Sequence Read Archive: DRR199260
            Assembly: GCF_014168915.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella sp. WP3-S18-ESBL-05
  ORGANISM  Klebsiella sp. WP3-S18-ESBL-05
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Klebsiella sp. WP3-S18-ESBL-05 isolated
            from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9350)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 334x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/02/2023 12:00:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,520
            CDSs (total)                      :: 5,397
            Genes (coding)                    :: 5,271
            CDSs (with protein)               :: 5,271
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 38 of 126
            Pseudo Genes (incomplete)         :: 94 of 126
            Pseudo Genes (internal stop)      :: 12 of 126
            Pseudo Genes (multiple problems)  :: 16 of 126
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022023.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9350
                     /organism="Klebsiella sp. WP3-S18-ESBL-05"
                     /mol_type="genomic DNA"
                     /strain="WP3-S18-ESBL-05"
                     /isolation_source="wastewater"
                     /db_xref="taxon:2675711"
                     /plasmid="pWP3-S18-ESBL-05_3"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6684 N 139.4278 E"
                     /collection_date="2018-08-06"
     gene            101..2176
                     /locus_tag="H7R64_RS27325"
                     /old_locus_tag="WP3S18E05_P30010"
     CDS             101..2176
                     /locus_tag="H7R64_RS27325"
                     /old_locus_tag="WP3S18E05_P30010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443153.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0008168 - methyltransferase activity [Evidence IEA];
                     GO:1904047 - S-adenosyl-L-methionine binding [Evidence
                     IEA]"
                     /GO_process="GO:0006306 - DNA methylation [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="WP_182945346.1"
                     /translation="MQTELIKELNKVLKVFPQFWSGENLHRSMVSDAISQKQPDLIKAL
                     VANKKIRSVYGTDINGTLIFDFDKLCSLLKYKEYWENSFTKYRNKIGLTSSGKYIADST
                     DIVLDFPFKDCLLEGGMTKEEQGKDEVYYNEIIARDEIDNLLSPKALVNVSRHTKDGEV
                     KNIHSFHEKDNLIIKGNNLIALHSLLGKFTGKVKLIYIDPPYNTGSDSFKYNDNFNHSS
                     WLTFIKNRLEVAKELLSEDGVIFVQSDDNEQAYLKVLMDGIFDRENFINMISVNMKNVA
                     GASGGGEDKRLKKNCEFILIYSKNYASLSAFKNVFEYTEIHEVVEQYRKEGKNWHYSSV
                     LVNAGEKIYVTSTVDGSGNEIKIYKHPNAVIKSVRQLMAQENISEKDVFYNFGTKIFEA
                     KDAQSSIRTRVAKAKKEHHVNDDILSIEYTPKSGKNKSQVYEQFYKGDKCRLFAWLRDI
                     TENIDGILYKKDKQGTYWDFTSHMNNLMKEGEISFQNGKKPEALIQRIIDISTNKDDIV
                     LDFHLGSGTTAATAHKMERRYIGIEQMDYINDITVPRLKNVIAAENNGISRDVSWQGGG
                     SFVYAELMELNAYFVHEIQKAQSNEELEKLFAVMKTEAHLNYQVELENVLSAEYEMDGI
                     PRKVAFRELELHEQKQLLIEILDKNQLYVNASDMDDSDLNISESDKAFTRSFYGME"
                     /name="site-specific DNA-methyltransferase"
     gene            2181..4817
                     /locus_tag="H7R64_RS27330"
                     /old_locus_tag="WP3S18E05_P30020"
     CDS             2181..4817
                     /locus_tag="H7R64_RS27330"
                     /old_locus_tag="WP3S18E05_P30020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443154.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0003677 - DNA binding [Evidence IEA]; GO:0016787 -
                     hydrolase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="WP_113382833.1"
                     /translation="MAKKPKSAGSEKLLFNKLRQFDEPGLFQDSYQTPDYIQANLKDPL
                     RPYQHGALRYLHYTQRKREEALLHYRHLLFHMATGAGKTMVMAGTILYLFKEYGYQNFI
                     FFVHTDAIIQKTRENLLNPQSPKYLFSQELEIDGEKITIAPVETFPSAPESNTIYLKLS
                     TIHKMHDELNSYRENSITYEDLKEIPLVLLGDEAHHFNAGTKAKGKSKTSPENEEQTWE
                     RTIENVLALRPDNRLFEFTATIDLANKDIGNKYRDRVVYQYDLKQFMSDGYSKKVMLLE
                     ANQNDGDKMLDAVLLSQYRKLVAADNGITGFKPVILFKSNKIAISKAKQEAFSQLIAAM
                     TPESVRRHLANKKVQLSSETSIWHKVIQRYAGSDLVTVIGQIQEDFNDFNLLNVNKSDL
                     LEENPVLLNTLEEVDNPVRAVFAVAKVNEGWDVLNLYDIVRISEQASNSKTGTDSEAQL
                     IGRGARYFPFGYEGKRSFTRRFDNSAKDLSVLEQLHYHTINEPAYIKTLHASLEQADID
                     VHQDGGGTIEHARLKEDFKKSAVYQSGKLYFNEVEEIESSSRNWETYSLETRFEIPYQT
                     AGEESLDNLTGATAGITKPEPLVLDARFYRKAMQRISFYALDNLQRFFPKLTGIREFIH
                     SDAYLGRLKITVTVPQSLDFATVPAKEKLHLLETVLLRIAENVRRNDQKVKGTYRFISQ
                     PVKEVIKDYSLHIDPSVIINQKITAAPTIGKRWYVYDNAILNQLEHRLVKTLEGFMPKL
                     KARYDDIYVLRNDEQSTRFKLTEFGGVRGFMPDFIMILTHHADNAYWQVFLEPKGDDRL
                     LDDAWKERMLETLNDRERIVIDENEDVRLVGIKFFANSQMDAFVSDMQNRLNEGESLET
                     ASLSLPL"
                     /name="DEAD/DEAH box helicase family protein"
     gene            complement(4856..5440)
                     /locus_tag="H7R64_RS27335"
                     /old_locus_tag="WP3S18E05_P30030"
     CDS             complement(4856..5440)
                     /locus_tag="H7R64_RS27335"
                     /old_locus_tag="WP3S18E05_P30030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobX"
                     /protein_id="WP_113382832.1"
                     /translation="MSDDIKQIAALIGHHQALEKRVTSLTEQFQDASAQLQQQSDTLSR
                     LIRELDSASGNMTETVRQSVHAALIQVEKELKQAGLAQQKPATEALNQATDTAKAMIHE
                     MRREMSRYTWKSAIYLVLTIFFVMAACVTAFTWFMNDGYSQIAEMQRMEAVWQKKAPLA
                     DISRCDGKPCVKVDTRATYGDKENTWMIIKK"
                     /name="mobilization protein MobX"
     gene            complement(5453..6550)
                     /gene="mobA"
                     /locus_tag="H7R64_RS27340"
                     /old_locus_tag="WP3S18E05_P30040"
     CDS             complement(5453..6550)
                     /gene="mobA"
                     /locus_tag="H7R64_RS27340"
                     /old_locus_tag="WP3S18E05_P30040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobA"
                     /protein_id="WP_040113349.1"
                     /translation="MASYHLSVKTGGKGSASPHADYISREGKYAREKDSDLEHKESGNM
                     PAWAAHKPTEFWKAADTFERANGCTYREIEIALPRELKPEQRLELVRDFVRQEIGDRHA
                     YQFAIHNPKAAIAGGEQPHAHIMFSERMNDGIDRDPQQYFKRANSKAPERGGAKKARFG
                     ETPTERKEHLVAQRERWADLQNKHLERYQHTDRVDARSLKAQGIEREPERHLGAGQVQR
                     FDTAQLQAILERRDAERQAEQSRDERDSVIDVTTSLREAISERDNLTIKQEQKPEPAQE
                     DTSGRVFDFEKEPEKLNALVSDALKDVQDEIDLQSLVNETMAEFQGIHQEMERLAEKQR
                     QQEKEQQRLAEQTRQKPDKGWSFSR"
                     /name="mobA"
     gene            6715..7182
                     /gene="traD"
                     /locus_tag="H7R64_RS27345"
                     /old_locus_tag="WP3S18E05_P30050"
     CDS             6715..7182
                     /gene="traD"
                     /locus_tag="H7R64_RS27345"
                     /old_locus_tag="WP3S18E05_P30050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_064645523.1"
                     /translation="MNKSEKQEKWAADRVQYIRGLKSPNEQQKLMLILTDKADKTAQDI
                     KTLSLLMKAEQAAEKAQEARAKVMNLIQAEKRAEARAARKARDHALYQSAGLLILAGLV
                     DSQTGKPVDDTAALLGALASLNDLSRDNPKWSDWKIRGQELLNSKKSDSSA"
                     /name="traD"
     gene            7779..8732
                     /locus_tag="H7R64_RS27350"
                     /old_locus_tag="WP3S18E05_P30060"
     CDS             7779..8732
                     /locus_tag="H7R64_RS27350"
                     /old_locus_tag="WP3S18E05_P30060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946646.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_113382830.1"
                     /translation="MSELVVFKANELAMSRYDLTEHETKLILCCVALLNPTVENPTRAD
                     RTVSFTYQQYAKMMGLTADNAYHRLNSATSELMTRTVEIIYPAGDVSKRIFQWVNLAEF
                     NRRTQSLSLVFSEDILPYLFQIKRFIKYNLEHVKSFENKYSMRIYEWLLKELTQRKTHK
                     ANIEISLKDFKFMLMLDKNYPEFKELNRWVLKAAMKDLNNHSNMKLSIEKRGRPADNLI
                     FQVELDKQIDLVSELAKESIPRSTKSAATTPESSLHSGLGKILHDALTAKIQLTNFEAK
                     FLSDMQSKYDLNGSFSWLSSKQQTTLEKILAKYGRL"
                     /name="replication initiation protein"
     gene            8740..9165
                     /locus_tag="H7R64_RS27355"
                     /old_locus_tag="WP3S18E05_P30070"
     CDS             8740..9165
                     /locus_tag="H7R64_RS27355"
                     /old_locus_tag="WP3S18E05_P30070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443152.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_113382829.1"
                     /translation="MALVSVTEAARLTGKNRATIHRYIKQGKLSQSTDAAGSKKIETSE
                     LLRVFGAISASPATALQCDVMQHDTTVENELPVAALQQKIAMLEQLLESKDKEIQRQDD
                     HIDSLKKAMLLIESKLPATQEPATPSMVKKSWQFWKK"
                     /name="helix-turn-helix domain-containing protein"
     Typing          7780..8732
                     /name="IncP"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.275"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5457..6550)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 attttttccg catctgcatc agcagtatcg ctgccaggtt tctgctcagg acgcgatgcc
       61 ctacaatcat ttcaatctct aacagtcata aggtaatgac atgcagaccg aattaataaa
      121 agaactgaac aaggttttaa aagtgtttcc gcaattctgg agcggagaaa atttacaccg
      181 ttcaatggtg agtgacgcca tcagtcaaaa gcagccagat ctaatcaaag cactggtcgc
      241 taataaaaaa atacgttctg tctacggcac agatattaac ggaactttaa ttttcgattt
      301 tgacaaactt tgcagcctgt taaaatataa agaatactgg gaaaacagct ttactaaata
      361 ccgaaataag attggattaa ccagctcagg taaatacatt gccgatagta ctgatatagt
      421 gcttgatttt cctttcaagg attgccttct tgaaggcgga atgacaaaag aagaacaagg
      481 gaaagacgag gtttattata acgagattat tgctcgcgat gaaatagata atcttctatc
      541 cccaaaagca ttggttaatg tatctcgcca tacaaaggat ggggaagtta aaaacattca
      601 tagcttccat gaaaaagata acttgataat aaagggaaat aatttaattg cacttcactc
      661 actattgggt aagtttactg gaaaagttaa attaatatat atagacccac catacaatac
      721 aggatctgac agttttaaat acaatgacaa tttcaatcat tcatcatggc taacatttat
      781 aaaaaataga ctagaagtag caaaagaact tcttagtgaa gatggagtca tcttcgtaca
      841 atcagacgac aatgaacaag cgtatttaaa agttttaatg gatggcattt ttgataggga
      901 aaactttatc aatatgatat cggttaacat gaagaatgtt gccggagcct ctggtggagg
      961 tgaggataaa agacttaaaa aaaactgtga gtttatttta atttactcaa aaaactatgc
     1021 ctccctttct gcatttaaaa atgtttttga atacacagaa attcatgagg tagttgagca
     1081 atataggaag gaaggtaaaa attggcacta ttcctccgta ctggtaaatg caggggaaaa
     1141 aatatacgta acctctactg ttgatggcag tgggaacgaa ataaagatat acaaacaccc
     1201 taacgcagta attaaatcag ttaggcaatt gatggcgcag gaaaatattt cagaaaagga
     1261 tgttttttat aactttggaa caaaaatatt tgaagcgaaa gatgctcaat catccattag
     1321 aacgcgagta gcaaaagcaa aaaaagagca tcatgttaat gatgatatat tatcaataga
     1381 atacacacct aaatcaggaa aaaataaaag tcaagtatat gaacaattct acaaagggga
     1441 taaatgtaga ttgtttgcat ggcttagaga tataactgaa aatatagatg gcattttata
     1501 caaaaaagat aaacaaggaa cgtattggga tttcacatca cacatgaaca atctaatgaa
     1561 agaaggtgaa atatcatttc aaaatggaaa aaaaccagag gcattaatac aaagaattat
     1621 agatatttca acaaataaag atgatattgt actagatttc catctcggaa gcgggacaac
     1681 agctgcaaca gcacataaaa tggaacgtcg ttacattggc attgaacaaa tggattacat
     1741 caatgacata acggttcctc gactaaaaaa tgtaatcgca gcggaaaaca atgggatttc
     1801 aagagacgtg agctggcagg gcggtggaag ctttgtatac gccgaactga tggagctgaa
     1861 cgcgtacttc gtgcacgaaa tccagaaagc acagtctaat gaagaactcg aaaaactctt
     1921 tgccgtgatg aaaacggagg cgcatctcaa ctatcaggtg gagctggaga acgtactgtc
     1981 cgctgaatac gagatggacg gtattcctcg taaagtcgcg ttcagagaac tggaactgca
     2041 tgagcagaag cagctactga ttgaaatcct cgataaaaat cagctctacg tgaacgcttc
     2101 cgatatggat gacagcgacc tgaacatctc agagagcgat aaagccttta cccgcagctt
     2161 ctacggtatg gagtaacggt atggcgaaga aaccaaagtc agccggcagc gagaagctgc
     2221 tgtttaacaa gctcagacaa ttcgatgaac ctgggctttt ccaggatagc taccagacgc
     2281 cggactacat tcaggcgaac ctgaaagatc ccctgcgccc ataccagcac ggcgcactcc
     2341 gttacctgca ttacacccaa cgcaaacggg aagaggcgct actgcattac cgtcatctgc
     2401 tgtttcacat ggcaaccggc gcaggaaaaa cgatggtgat ggcgggtacg atactctatc
     2461 tgtttaaaga gtacgggtat cagaacttca tcttcttcgt gcataccgac gcgattatcc
     2521 agaaaacccg cgagaacctg ctgaacccgc agtcgccgaa gtacctgttc agtcaggaac
     2581 tggaaatcga cggggagaaa atcaccatcg caccggtgga gacgttccca tctgcgcctg
     2641 agagcaacac gatttacctc aagctctcaa ccatccataa gatgcacgac gagctgaaca
     2701 gctatcgtga gaacagcatc acttacgagg atttgaaaga aatcccgtta gtccttctcg
     2761 gtgacgaggc gcatcacttc aacgccggca ccaaagcgaa aggcaagtcg aagacctcac
     2821 cggaaaacga ggagcagacc tgggagcgca cgattgagaa cgttctcgct ctgcggccgg
     2881 ataaccgcct gttcgagttt accgccacca ttgacctggc gaataaagat atcgggaata
     2941 aataccgtga cagagtggtg tatcagtatg acctgaaaca gttcatgtca gacggttact
     3001 cgaaaaaggt catgctgctg gaagcgaatc agaacgacgg cgacaagatg ctcgatgccg
     3061 tgctgttaag ccagtaccgc aaactggtgg cggcggataa cggcatcacc ggcttcaaac
     3121 cggttattct gttcaagtcg aacaagattg cgatatcgaa ggcaaagcag gaggcgtttt
     3181 cgcaactgat tgcggcaatg acgcctgaga gcgtcagacg ccatctggcg aataaaaaag
     3241 ttcagctcag ctctgaaacc agtatctggc ataaagtgat tcagcgttac gctggcagcg
     3301 atttagtgac ggttatcggg cagatccagg aggacttcaa cgacttcaac ctgcttaatg
     3361 tgaataagtc agacctgctg gaagaaaacc ccgttctgct gaacacgctg gaagaagtcg
     3421 ataatccagt gagagccgtc ttcgccgtgg cgaaggtcaa cgaaggttgg gacgtcctca
     3481 acctctacga tatcgtccgc atcagcgagc aggcgagcaa cagcaaaacc ggcacggaca
     3541 gcgaggcaca gcttatcggt cgcggtgcac gttacttccc gttcgggtat gaaggtaagc
     3601 ggagcttcac ccgccggttt gataacagcg cgaaggattt gagcgtcttg gaacaactgc
     3661 actaccacac catcaatgag ccggcgtaca tcaagacgct gcacgcgtct cttgagcagg
     3721 cggacattga cgtacatcag gacggcggcg gtacgattga acacgccaga ctgaaagagg
     3781 actttaaaaa gtccgccgtg tatcagagcg ggaagctgta cttcaacgaa gtggaagaaa
     3841 ttgagagcag cagccgcaac tgggaaacct actcactgga aacgcgcttt gagataccgt
     3901 atcagaccgc cggtgaggag tcgctggaca acctgacggg ggcaacggct ggcataacaa
     3961 aaccggaacc cctggtgctg gacgcgcgtt tttaccgcaa ggcgatgcag aggatcagct
     4021 tctacgcgct ggataacctg caacgctttt tcccaaaact gaccggcatc cgtgagttca
     4081 tccacagtga cgcctacctt ggcaggctga aaatcaccgt caccgtaccg cagtcactgg
     4141 atttcgccac cgtaccggca aaagagaaac tgcacctgct ggaaaccgtt ctgctgcgta
     4201 tcgcagagaa cgtgcgccgc aatgaccaga aagtaaaggg aacataccgc tttatcagtc
     4261 agccggtgaa agaggtgatt aaagactaca gcctgcatat tgacccgtcg gtcattatca
     4321 accagaaaat cacagcagca cccaccatcg gtaaaagatg gtacgtgtat gacaatgcga
     4381 tactgaacca gcttgagcat cgtctggtga aaacgctgga aggctttatg ccgaagctca
     4441 aggcacgtta cgacgatatt tacgtgctgc gtaatgacga gcagtcaacg cgctttaagc
     4501 tcacggagtt cggcggcgta cggggcttta tgccggattt catcatgata ctgacgcacc
     4561 atgcggacaa tgcttactgg caggtattcc ttgagccgaa aggggatgac cgtctgctgg
     4621 atgacgcatg gaaagaaagg atgctcgaaa ccctgaatga cagggaacgc attgtgattg
     4681 atgaaaatga ggacgtccgg ctggtcggga ttaaattctt tgccaacagt cagatggacg
     4741 ccttcgtcag cgacatgcaa aaccggctga atgagggcga atcgctggaa accgcttcgc
     4801 tctccctgcc gctgtaattc acgacaacaa taacggcgca atgtgcgccg ttatgttatt
     4861 ttttgataat catccaggta ttctctttat caccatacgt agctcgggtg tccaccttaa
     4921 cgcagggctt tccgtcacaa cgcgatatat cagccagcgg ggctttcttc tgccagaccg
     4981 cttccatccg ttgcatttca gcaatctggc tgtacccgtc attcataaac cacgtaaatg
     5041 ccgtcacgca ggcagccatg acgaaaaata tcgtcagcac gagataaatc gcggatttcc
     5101 acgtatagcg cgacatctcg cgtcgcattt catggatcat cgccttcgcc gtatcggtag
     5161 cctgattaag cgcttctgtc gccggtttct gctgtgccaa tccggcttgt ttcagctctt
     5221 tctcaacctg aatgagcgcg gcatgaacag attgcctgac ggtttcagtc atattaccgg
     5281 acgcgctgtc cagctcacga atgagcctgc tcagcgtgtc actctgctgc tgtaattgtg
     5341 ctgaggcgtc ctgaaactgc tcagtcagag aggtaacacg cttttccagc gcctgatgat
     5401 gcccgatgag ggcagcgatt tgtttaatgt cgtcgctcat gaaaaatcct gtctatcgtg
     5461 aaaatgacca gcctttatcc ggcttctgtc gtgtctgttc ggcgagtctc tgctgttctt
     5521 tctcctgctg acgctgtttt tctgccagtc gctccatttc ctgatgtatc ccctgaaact
     5581 ccgccattgt ctcgttcaca agggactgaa ggtcgatttc gtcctgtacg tccttcagcg
     5641 catcactgac cagtgcgttc agcttctccg gctctttctc aaaatcgaaa accctgccgg
     5701 aagtgtcttc ctgcgcaggt tcaggcttct gctcttgttt tatcgtcagg ttatcccgct
     5761 cgctgatggc ttcccgtagc gaggtggtca cgtcaatcac actgtcacgt tcatcacgag
     5821 actgctccgc ctgcctttca gcgtccctgc gctcaaggat ggcctgtaac tgcgcagtat
     5881 cgaaccgctg aacctgtccc gcgccaagat gccgctcagg ctcacgctca atgccctgag
     5941 ctttgagtga gcgtgcatca acgcggtcgg tatgctgata ccgttcaagg tgcttattct
     6001 gtaggtcagc ccagcgttcc cgctgggcga cgaggtgctc tttgcgttct gtcggcgttt
     6061 ccccgaaacg tgcctttttc gcaccgccgc gctcaggggc tttgctgtta gctcgcttaa
     6121 aatactgctg cgggtcacgg tcgataccgt cattcatccg ctcagagaac atgatatgcg
     6181 cgtgcggctg ctcgccgccg gctatcgccg ctttcggatt atgaatggcg aactgatacg
     6241 catgacggtc gccgatttcc tgccggacaa aatcgcggac cagttcaagg cgttgttcgg
     6301 gtttcagctc acgcggcagg gcaatctcga tttcacggta ggtacagccg ttggcacgtt
     6361 caaacgtgtc agcggctttc cagaactccg tcggtttatg cgctgcccag gcaggcatat
     6421 tgccggactc cttgtgctca aggtcggagt ctttttcacg ggcatacttt ccctcacgcg
     6481 aaatataatc ggcatgggga gaggcactgc ctttcccgcc ggtttttacg ctgaggtgat
     6541 aggaggccat cgtgttttta tcccgctgaa ggcgcgcacc gtttctgaac gaagtgaaga
     6601 aacgtctaag tgcgcactga taataaaaag cttatcagtg attctacctg catttgacct
     6661 cggccgccat tcctgaccat aatttccaca tcaacattca gaggataaat gggcatgaat
     6721 aaaagcgaaa aacaggaaaa atgggcagca gacagagtgc agtacattcg cggcttaaag
     6781 tcgccgaatg aacagcagaa actcatgctg atactgacgg ataaagcgga taaaacagcg
     6841 caggatatca aaacgctgtc cctgctgatg aaggctgaac aggcagcaga aaaagcgcag
     6901 gaagccagag cgaaagtcat gaacctgata caggcagaaa agcgagccga agccagagcc
     6961 gcccgtaaag cccgtgacca tgccctgtac cagtcagccg gattgctcat ccttgcgggt
     7021 ctggttgaca gtcagacggg taagcctgtt gatgataccg ctgccttgct gggtgcatta
     7081 gccagtctga atgacctgtc gagggataat ccgaagtggt cagactggaa aatcagaggt
     7141 caggaactgc tgaacagcaa gaagtctgac agctctgcat agcagacctg ccataaaacg
     7201 ccctgagaag cccgtgacgg gcttttcttg tgtcatgggt agatttcctc gtgtgagtcc
     7261 atgaaaagcg cctgtagcat catttacccc tattccctgc cagagctgtg agcgaagcga
     7321 actgattttc acagagataa cagcgattca ggtgctggat ggtcggagac aaaagaaatg
     7381 ttcagcgaat tgcccgcgct agcgcgggtg ctatctcagt atttttgggg tttaagatct
     7441 gttttgaagc ggagcaaact gcgtttgcga catcctttta tcgtatcgtt caactgactc
     7501 aggcgtgcag ttatccacaa ggcggggatc tattcttttt tatctttttt attctttctt
     7561 tattctagtg tttttaatta gttgatttat ttaaaaaaat aaacagaaag gtgtagtaaa
     7621 aatcatggca ggtgtagtaa aaatcataag taatctcaga aaggtgtagt aaaaatcata
     7681 atatacacag cacgacaaca aaggtgtagt tgacaaaaac cgctacacca ttactatcag
     7741 gtgtaggttt ttatcttacc tacaccaagt gagggtcgat gtctgagctg gtagttttta
     7801 aagctaacga attagctatg agtcgctacg atttaaccga gcatgaaaca aaactgattt
     7861 tgtgttgtgt ggcattacta aatccaacag tggaaaaccc tacaagagca gaccgtactg
     7921 tctcattcac ctatcagcag tatgcaaaaa tgatggggtt aacagctgat aacgcttatc
     7981 atcgattaaa cagtgcaacc agtgagttaa tgactcgaac cgtagagatc atttatccgg
     8041 ctggagacgt ctctaagaga atattccaat gggttaatct ggctgagttt aatcgtcgaa
     8101 cacaatcatt aagcttagtt tttagcgaag atatattgcc ctatctattt caaataaaga
     8161 gatttattaa gtataacctt gagcatgtta agtcttttga aaataaatac tctatgcgca
     8221 tatatgaatg gcttttaaag gagctaacac aaaggaaaac gcataaggca aatatagaaa
     8281 taagtcttaa agattttaag ttcatgttaa tgcttgataa aaattaccct gagtttaaag
     8341 agcttaatcg ctgggttttg aaggctgcta tgaaagatct aaacaatcat agcaatatga
     8401 agttatccat tgaaaaacga ggccggcccg cagacaacct gatatttcag gttgagttag
     8461 ataaacagat cgaccttgtt tcagagcttg ctaaggaatc tattcctaga tcgacaaaat
     8521 ccgcagctac tacaccagaa tctagcctac atagtgggct aggaaaaata ttacacgatg
     8581 cgctcacagc aaaaattcag ttaaccaatt tcgaagctaa gtttttgagt gacatgcaaa
     8641 gcaaatacga cctgaatggc tctttctcat ggctttcctc aaaacaacag acaacccttg
     8701 agaaaatttt agccaagtac ggacgactct gaggtatcta tggcgctggt atctgtaaca
     8761 gaagcagcta gattgactgg caaaaacaga gcaacaatac atcgctacat taagcaagga
     8821 aaactgtcgc aatctacaga tgcagcagga tcaaaaaaga tagaaacgtc agagctttta
     8881 cgtgtttttg gtgctatctc tgcatcacct gcaacagcgt tgcaatgcga cgtaatgcag
     8941 cacgatacaa cagtagaaaa tgagttacct gttgcagcac tgcaacaaaa aatcgccatg
     9001 ttagaacagc tactggagag caaagataag gaaatacagc gtcaggatga ccatatcgac
     9061 agccttaaaa aagccatgct gctaatcgaa tccaagctgc cagcaacaca ggaacctgcc
     9121 acgccgtcga tggtaaaaaa atcatggcaa ttctggaaga aatagcggtt tcatccggtt
     9181 taccggctga aaccagcctc gccattctga taacaaacta gcaacacaaa aaacgcccct
     9241 ttttgggcag gaaaactccc gcttttggac gttccggcgg gttttggcag tgaatggtgt
     9301 tcgggtggtg cgggtaagat cccttatgtt aaataccacc tgttttggtg
//
