LOCUS       NZ_AP022012             2924 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella quasipneumoniae strain WP3-S18-CRE-02 plasmid
            pWP3-S18-CRE-02_8, complete sequence.
ACCESSION   NZ_AP022012
VERSION     NZ_AP022012.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194418
            Sequence Read Archive: DRR199254
            Assembly: GCF_014168875.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Klebsiella quasipneumoniae str.
            WP3-S18-CRE-02 isolated from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 347x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 08:52:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,876
            CDSs (total)                      :: 5,753
            Genes (coding)                    :: 5,554
            CDSs (with protein)               :: 5,554
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 71 of 199
            Pseudo Genes (incomplete)         :: 121 of 199
            Pseudo Genes (internal stop)      :: 37 of 199
            Pseudo Genes (multiple problems)  :: 26 of 199
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022012.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WP3-S18-CRE-02"
                     /isolation_source="wastewater"
                     /db_xref="taxon:1463165"
                     /plasmid="pWP3-S18-CRE-02_8"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6684 N 139.4278 E"
                     /collection_date="2018-08-06"
     gene            complement(101..592)
                     /locus_tag="H7R71_RS29220"
                     /old_locus_tag="WP3S18C02_P70010"
     CDS             complement(101..592)
                     /locus_tag="H7R71_RS29220"
                     /old_locus_tag="WP3S18C02_P70010"
                     /inference="COORDINATES: protein motif:HMM:NF017542.3"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_063660750.1"
                     /translation="MLKEHAKIDNVLDFDLSLNTVEEINKMQEKGDVKVTTIVQNEKKV
                     SLKDQFVMAFTSNLRALAELNISQNELKVITYILEVMEFGNLISINQTSIAKELGLHKS
                     NVSYTFKRLVEKNILIKKDGHTFMNSNLFAKGLNHSMNEERRANLRSAQVENEHFKRSF
                     "
                     /name="replication/maintenance protein RepL"
     gene            1589..2740
                     /gene="mobV"
                     /locus_tag="H7R71_RS29225"
                     /old_locus_tag="WP3S18C02_P70020"
     CDS             1589..2740
                     /gene="mobV"
                     /locus_tag="H7R71_RS29225"
                     /old_locus_tag="WP3S18C02_P70020"
                     /inference="COORDINATES: protein motif:HMM:NF013258.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_096216837.1"
                     /translation="MYAICRVGKIKSQSQLSQAQSHNLRQRETFNADPLKTSNNEILYG
                     SPFVRDDVNKRLEESGVKIRKDSVLCAEMILTASPEFFNTDNSFKEWKNENLNFLKNKY
                     GENLINVIIHNDEKTPHLHAIITPIISDKNNNRLSMKEFLGGKKLLSELQTDYAKSMEK
                     FGLSRGIEKSVAKHQDIKTFYSSIEDAKEIGQETKEEFKKDFKVAINIKTPTPTGRIFK
                     TITPEEAKKSTIENLQEYKKKVNEKIHPMHDGIVNLSIENKRLQTENTQLREVAARLNR
                     DNEISRKRDQKFDHMRKLCPDEYNQLQKAYKNRLNEIEQKKKQDEELKLKQKLELEQKR
                     KEELAKRPKGESSLDLDEQRKNKMKPEPEPQQQIEYSRPRPRF"
                     /name="mobV"
ORIGIN
        1 aagctccgta gatcggagct tttaatttat ataaattaaa agggatttgc aaaatccctt
       61 tttttattgt tataatcaaa ttgcgtattg agttttattt ttaaaagctt cttttgaaat
      121 gttcattttc aacttgagcg gatcgtagat tcgctcttct ttcttcgttc atactatgat
      181 ttaacccctt tgcaaataag ttagagttca taaatgtatg accatctttt tttattagaa
      241 tatttttttc tacaagtctc ttaaaagtat atgaaacatt ggatttatgt aatccaagct
      301 ctttagctat tgatgtttga ttaatgctaa tgaggtttcc aaactccata acttcaagaa
      361 tataagttat tactttaagc tcattttgac taatatttaa ctcagctaat gctcttaagt
      421 tagaagtaaa agccataacg aactgatcct ttaaagaaac ctttttttcg ttttgcacta
      481 ttgtagtgac ttttacgtca cctttttctt gcatcttgtt tatttcttca actgtattta
      541 gagacaaatc gaaatctaaa acgttatcta tttttgcatg ttcttttaac atataacctc
      601 cttatttaat cgtaatatat aaaagacata ttatttaaat aaccgatcat aaatatttgt
      661 caataactaa aacaaaaaaa ctataacaaa gttatagatt tttgaacata tgactaaacc
      721 aaaaaagtta ttgataccta taactggagt tattgatgcc tataacaatg agttattgat
      781 acctataaca atagttattg atacctataa ctttttaagg ctcaaagcct tgtgggacaa
      841 gggctagagg ggtggttaag aagtaagatt ttagtaagaa acataagaaa gggtaaaacc
      901 gccctcgccg ggcaggccaa cccctcgaac cacagtagca agctactgta gttctcgcgg
      961 ttctcgattg gttcttctaa ctctggctaa tactcttttg gccataggcc atactgccgc
     1021 ctaaatgatg tctcaaacat catcaaaagc ggcctgcagg tttaccggac taaacattac
     1081 agacccgtca gagggtagtt tttcaggctc ttatgaggtc atagccctaa aactcatcac
     1141 agaccgttac agagcgtttt agagggggtt atggtatcta tccagaaaga cccgtcagag
     1201 gggtaggatc aattggtttt aaattggtaa tcgtgaacaa gtgaaaaatt tatttttcgc
     1261 ttgtgaggga ttgatctttt gactttatat tttcagcttt aactaatttg caatgttgct
     1321 attttcctat tctgtgttaa ttaaaaagta taacggtaat ctgtaaaggt acactcacta
     1381 caccttactt cgtaaggttc cgttcgtgca cagctctaca tggagaattt aaaatggtaa
     1441 tagttatagt tttggaaatg ttatttggtt ttatgttgat gggttggatt atttctagct
     1501 gtatttccac taaagataaa agccaagaaa ttagaaatag ttatagtgaa ataagtaaat
     1561 taaaaaagaa aataaaaggt aggtaaatat gtatgcaatt tgtagagtag ggaaaattaa
     1621 atcacaatct cagctttcac aagctcaaag ccataattta agacagcgtg aaacattcaa
     1681 tgcagatcca ttaaaaacaa gtaataatga aattttatat ggttctccgt tcgttcgtga
     1741 tgatgtaaat aaaagactcg aagagtcggg agtgaaaata agaaaggatt ctgtattatg
     1801 tgctgagatg attttaactg catctcctga attctttaat acagataata gttttaaaga
     1861 gtggaaaaat gaaaatttaa atttcctgaa aaataaatac ggtgaaaatt taattaatgt
     1921 cattattcat aatgatgaaa aaacaccaca ccttcatgca ataattactc ctattatttc
     1981 agataaaaac aataatagat tatcaatgaa agaattctta ggtggtaaaa agttattaag
     2041 tgaacttcaa acagattatg caaaatctat ggaaaaattc gggttaagca gaggtataga
     2101 aaaatcagtt gctaaacatc aagatattaa gactttttat tcttcaattg aagatgcaaa
     2161 agaaataggg caagaaacaa aagaagaatt caagaaagat tttaaagtag caattaatat
     2221 taaaacccca acacctacag gtagaatttt taaaacaata actcctgaag aagctaaaaa
     2281 aagtacaatt gaaaatcttc aagaatataa gaaaaaagta aatgaaaaaa ttcaccccat
     2341 gcatgatggt atagtaaatt tatcaattga aaacaagagg ttacaaactg agaacacgca
     2401 gttacgtgaa gtggctgcaa gactaaatag agacaatgaa atatcaagaa aacgagatca
     2461 aaaatttgat cacatgagaa aactttgtcc tgatgaatat aatcaattac aaaaagcgta
     2521 taaaaatagg ctcaatgaaa ttgagcaaaa aaagaaacaa gatgaagagt taaaattaaa
     2581 acaaaaatta gagttagagc aaaaaagaaa agaagaatta gcaaaaagac caaaaggtga
     2641 aagtagtctt gatcttgatg aacaacgtaa aaacaaaatg aagccagaac ctgaaccaca
     2701 gcaacaaata gaatattcaa gaccaagacc cagattttaa gggggaaccc ctaaacccgt
     2761 tccccctcgc actatctgcg taatctcgca gatagattgc tcacccccac ccccacttag
     2821 acaagaagaa cttgtctaag cgacccgaac cataataata aatataaagg gcgtagccgc
     2881 ctgctgcgga gctgctggca atgatggatt tttcaaaaat aatt
//
