LOCUS       NZ_AP022000             6669 bp    DNA     circular CON 14-JUN-2023
DEFINITION  Escherichia coli strain WP3-W18-ESBL-07 plasmid pWP3-W18-ESBL-07_2,
            complete sequence.
ACCESSION   NZ_AP022000
VERSION     NZ_AP022000.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194413
            Sequence Read Archive: DRR199249
            Assembly: GCF_014168835.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Escherichia coli str. WP3-W18-ESBL-07
            isolated from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6669)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 176x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/14/2023 05:23:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,032
            CDSs (total)                      :: 4,919
            Genes (coding)                    :: 4,710
            CDSs (with protein)               :: 4,710
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 83 of 209
            Pseudo Genes (incomplete)         :: 146 of 209
            Pseudo Genes (internal stop)      :: 41 of 209
            Pseudo Genes (multiple problems)  :: 54 of 209
            Pseudo Genes (short protein)      :: 3 of 209
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP022000.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="WP3-W18-ESBL-07"
                     /isolation_source="wastewater"
                     /db_xref="taxon:562"
                     /plasmid="pWP3-W18-ESBL-07_2"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6684 N 139.4278 E"
                     /collection_date="2018-02-13"
     gene            complement(101..292)
                     /locus_tag="H7Q99_RS24810"
                     /old_locus_tag="WP3W18E07_P20010"
     CDS             complement(101..292)
                     /locus_tag="H7Q99_RS24810"
                     /old_locus_tag="WP3W18E07_P20010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            357..680
                     /locus_tag="H7Q99_RS24815"
                     /old_locus_tag="WP3W18E07_P20020"
     CDS             357..680
                     /locus_tag="H7Q99_RS24815"
                     /old_locus_tag="WP3W18E07_P20020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            889..>1320
                     /locus_tag="H7Q99_RS25285"
                     /pseudo=""
     CDS             889..>1320
                     /locus_tag="H7Q99_RS25285"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1358..1876
                     /locus_tag="H7Q99_RS24825"
     CDS             1358..1876
                     /locus_tag="H7Q99_RS24825"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            1883..2137
                     /locus_tag="H7Q99_RS24830"
     CDS             1883..2137
                     /locus_tag="H7Q99_RS24830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015059032.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2455..2874)
                     /locus_tag="H7Q99_RS24835"
                     /old_locus_tag="WP3W18E07_P20040"
     CDS             complement(2455..2874)
                     /locus_tag="H7Q99_RS24835"
                     /old_locus_tag="WP3W18E07_P20040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(2955..3392)
                     /locus_tag="H7Q99_RS24840"
                     /old_locus_tag="WP3W18E07_P20050"
     CDS             complement(2955..3392)
                     /locus_tag="H7Q99_RS24840"
                     /old_locus_tag="WP3W18E07_P20050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            3652..5220
                     /gene="cea"
                     /locus_tag="H7Q99_RS24845"
                     /old_locus_tag="WP3W18E07_P20060"
     CDS             3652..5220
                     /gene="cea"
                     /locus_tag="H7Q99_RS24845"
                     /old_locus_tag="WP3W18E07_P20060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            complement(5217..5444)
                     /locus_tag="H7Q99_RS24850"
                     /old_locus_tag="WP3W18E07_P20070"
     CDS             complement(5217..5444)
                     /locus_tag="H7Q99_RS24850"
                     /old_locus_tag="WP3W18E07_P20070"
                     /inference="COORDINATES: protein motif:HMM:NF015489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_229471606.1"
                     /translation="MLYAIVISTILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            5606..5743
                     /locus_tag="H7Q99_RS24855"
                     /old_locus_tag="WP3W18E07_P20080"
     CDS             5606..5743
                     /locus_tag="H7Q99_RS24855"
                     /old_locus_tag="WP3W18E07_P20080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     Typing          complement(6029..6584)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.52"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          671..2241
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.618"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cccgacaccc gctaaaacct gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
       61 ctcacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
      121 ccccgaaacg tgcgaggcag ctgcggtaaa gctcatcggc gtggtcgtga agcgattcac
      181 agatgtctgc ctgttcatct gcgtccagtt cgttgagctt ctccagcagc gttaatgtct
      241 ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttagtc actgatgcct
      301 ccgtgtaagg gggtatttct gttcatgggg taatgatacc gatgaaacgc gagaggatgc
      361 tcacgatacg ggttactgat gatgaacatg ccaggttact tgaacgctgt gagggtaaac
      421 aactggcagt atggatgcgg cgggtctgcc tgggtgagcc ggttgcccgt tccgggaagc
      481 tgccgacact ggcaccgccg ttactgcgtc agctggccgc catcgggaat aacctgaatc
      541 agacagcgag gaaggtgaac agcgggcagt ggtcttccgg tgaccgggtt caggtggtgg
      601 ccgcactgat ggccatcggg gatgagctgc gccggctgcg tctggccgtc agggaacagg
      661 gggcgcggga tgatagttaa atttcatgcc aggggaaaag gtggtggcag tggtccggtt
      721 gattacctgc tggggaggga gcgtaaccgc gaaggcgcaa cggtgcttca gggtaatccg
      781 gaagaagtcc gggaactcat cgatgccacg ccatttgcga agaaatacac gtccggtgtt
      841 ctgtcgttcg cggagaagga gctgccgccg ggaggacgtg aaaaagtgat ggcgagcttt
      901 gagcgtgtac tgatgcccgg tctcgaaaaa aatcagtaca gcatcctgtg ggtggagcac
      961 caggacaagg gacggcttga gctgaatttt gtcattccga acatggagct acagaccgga
     1021 aaacgcctcc agccgtacta cgaccgcgca gacaggccta gaattgatgc ctggcagacg
     1081 ctggtgaatc accattacgg gctgcatgac ccgaacgccc cggagaaccg caggacgctg
     1141 acactccctg ataacctgcc tgaaacgaaa caggcgcttg ctgagggcgt cacgcgaggt
     1201 atagatgcac tttaccatgc cggagagata aaaggccgtc aggatgtgat tcaggcgctc
     1261 actgaggcgg ggctggaagt ggtcagggtg acgcgaagca gtatcagcat tgcagatccg
     1321 aacggcggga agaatatcag gctgaaagga gcattttatg agcaatcttt tgcagacggg
     1381 cgcggagttc gagaaaaagc tgaaagagag agccgaatct acagagaaaa tgctgaacaa
     1441 cgagttcagg aggctcggcg aatctgtaag cgaggctgtg acatcaaacg agacgaaaat
     1501 cagagacgct atagccctgt tcacagcctc gacagaggaa tcgctggaaa aacaccggga
     1561 aggggtgaaa gaggcgatga tgcagcacag gagggacgtg ttaaagctgg cagggaatac
     1621 gggcatgatg ttactgggga tagtctttct cctgtttacc gcgagtggcg ggacgctctg
     1681 gtatcttgga gggaggatac aggcgaacct ggaagaaatc aggaagcagg aagagacatt
     1741 gcagaaactg aacgcgaaga catggggcgt ggagtttgtg caggacggga acaggaaatt
     1801 ccttgtcctt ccgtacggga aatcagcgga ggtgattccc tttcagggga aagagtgggt
     1861 acatctgaag gagtgacaca gagtgacaga gctggaaaca catttgctga acgccttaga
     1921 gcagctgcaa caggactata tgcagcggct gagcgaatgg gagagcgcct tcgtggaatt
     1981 gcagaagatg ttttcgctta cgcaacggga caacgcgatg ctgaacgagc gggtcatgca
     2041 gttgagtcag caggtgcagc acttgagcga gcagacagaa cgcttgagcg agcagacaga
     2101 acgcttgagc cagttataca gcgagaactg gagataagag aggaacggct gatacaggag
     2161 cgcgaacatg tgttatccct ggaacgggag cgtcagctgg aaatacagga acgcacgctg
     2221 gatggccctt cgctgggatg gtgaaaccat gaaaaatggc agcttcagtg gattaagtgg
     2281 gggtaatgtg gcctgtaccc tctggttgca taggtattca tacggttaaa atttatcagg
     2341 cgcgatcgcg gcagtttttc gggtggtttg ttgccatttt tacctgtctg ctgccgtgat
     2401 cgcgatgaac gcgttttagc ggtgcgtaca attaagggat tatggtaaat ccacttactg
     2461 tctgccctcg tagccatcga gataaaccgc agaaatcgtg tcagccagca gcctggattt
     2521 tttcggggta aggtctttgc tgcggtaatc agtacccgca aattgagttg tgccccggag
     2581 tgagtttaaa aattcctggc tgtgaaactg ctgaatgcgt tgttgcacat cactccgtga
     2641 ttttcctgtc gctttatctt tagctcccat atcccagaac tgccgaaaaa aaggcaccat
     2701 cattcgtgtt gtgtcggctt tttgggtgga aaagttggga tcaaaaccgt catcagaagc
     2761 atatacgtaa tgccgggtat gttgttccac gctgcccttt ggggtacagg ctgtgagagc
     2821 tactgttgct gttatcatgg cgataagaca aagtttgttt ccggtacgtc tcatgagtgt
     2881 ctccctacct taaagtattt attctcggtg agttattatc cgaagactat tttatcatca
     2941 gtctctgaca gcgttcagcg accgaacaac ctggaccaga atccccgttt cttttccagt
     3001 tccagtgcct gcctgagctg ggctatctca ttttgtagct gttcccgttc tgcttcctgg
     3061 cgtctgcgat ccatatcctg tgcctgttta tcctcaagca tcagcgtcag gcattgtttc
     3121 agctcattca gctcccggag aatgcgttct gtctgctgat catgtggatt ttctgcatgt
     3181 ccctcactgt gcctttctgg tgtctcattc tgctttaatt cgccgtatgc ccggatcagt
     3241 tcactggttt caaactcccg tcgtccgtcc ctgccggtac ggtaactgac aaggcctgcg
     3301 cgcatatcac ggtaaaactg tgaacgcgat ctgcctgtca tttttagtgc gtcccggagt
     3361 gtgtgccatg ccataaagtg acagtgtccc atagatgtct catctcatag tttcagtaaa
     3421 acataatgag gtctgagaac ggtaatgttt gtgctggttt ttgtggcatc gggcgagaat
     3481 agcgcgtggt gtgaaagact gtttttttga tcgttttcac aaaaatggaa gtccacagtc
     3541 ttgacaggga aaatgcagcg gcgtagcttt tatgctgtat ataaaaccag tggttatatg
     3601 tacagtattt atttttaact tattgtttta aaagtcaaag aggattttat aatggaaacc
     3661 gcggtagcgt actataaaga tggtgttcct tatgatgata agggacaggt aattattact
     3721 cttttgaatg gtactcctga cgggagtggc tctggcggcg gaggtggaaa aggaggcagt
     3781 aaaagtgaaa gttctgcagc tattcatgca actgctaaat ggtctactgc tcaattaaag
     3841 aaaacacagg cagagcaggc tgcccgggca aaagctgcag cggaagcaca ggcgaaagca
     3901 aaggcaaaca gggatgcgct gactcagcgc ctgaaggata tcgtgaatga ggctcttcgt
     3961 cacaatgcct cacgtacgcc ttcagcaaca gagcttgctc atgctaataa tgcagctatg
     4021 caggcggaag cagagcgttt gcgccttgcg aaagcagaag aaaaagcccg taaagaagcg
     4081 gaagcagcag aaaaggcttt tcaggaagca gaacaacgac gtaaagagat tgaacgggag
     4141 aaggctgaaa cagaacgcca gttgaaactg gctgaagctg aagagaaacg actggctgca
     4201 ttgagtgaag aagctaaagc tgttgagatc gcccaaaaaa aactttctgc tgcacaatct
     4261 gaagtggtga aaatggatgg agagattaag actctcaatt ctcgtttaag ctccagtatc
     4321 catgcccgtg atgcagaaat gaaaacgctc gctggaaaac gaaatgaact ggctcaggca
     4381 tccgctaaat ataaagaact ggatgagctg gtcaaaaaac tatcaccaag agccaatgat
     4441 ccgcttcaga accgtccttt ttttgaagca accagacgac gggttggggc cggtaagatt
     4501 agagaagaaa aacaaaaaca ggtaacagca tcagaaacac gtattaaccg gataaatgct
     4561 gatataactc agatccagaa ggctatttct caggtcagta ataatcgtaa tgccggtatc
     4621 gctcgtgttc atgaagctga agaaaatttg aaaaaagcac agaataatct ccttaattca
     4681 cagattaagg atgctgttga tgcaacagtt agcttttatc aaacgctgac tgaaaaatat
     4741 ggtgaaaaat attcgaaaat ggcacaggaa cttgctgata agtctaaagg taagaaaatc
     4801 ggcaatgtga atgaagctct cgctgctttt gaaaaataca aggatgtttt aaataagaaa
     4861 ttcagcaaag ccgatcgtga tgctattttt aatgcgttgg catcggtgaa gtatgatgac
     4921 tgggctaaac atttagatca gtttgccaag tacttgaaga ttacggggca tgtttctttt
     4981 ggatatgatg tggtatctga tatcctaaaa attaaggata caggtgactg gaagccacta
     5041 tttcttacat tagagaagaa agctgcagat gcaggggtga gttatgttgt tgctttgctt
     5101 tttagcttgc ttgctggaac tacattaggt atttggggta ttgctattgt tacaggaatt
     5161 ctatgctcct atattgataa gaataaactt aatactataa atgaggtgtt agggatttaa
     5221 ttattcttta ttgatataaa aagtcctagc aatccaaatg ggattgctag gaccaaacaa
     5281 agtagattat atagcataaa taggtttaat tttgctacgg gggcgttatt taggtttttt
     5341 cttctttcga aaaaatcttt ctttatgaag ttaaaagcta tgtattcaat agcatatttt
     5401 gaatatggac atagaatagt gcttatcact attgcatata gcatcttatc tgacacaagg
     5461 aaataatacc cttcgctgtt ttttgttata aggtatatat atataagtgt gcagtacagg
     5521 ccaaataaaa tattttttat gtagtatctt aagctcataa attaaacctc gccatatatt
     5581 cttttcattt tataaggatc gagttatgag gaaaagattt tttgtgggaa tattcgcgat
     5641 aaacctcctt gttggatgtc aggctaacta tatacgtgat gttcagggag ggaccatcgc
     5701 accatcctcc tcttctaaac tgacggggat cgcggttcag tagaaaagat caaaggatct
     5761 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     5821 ccaacggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     5881 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtc gggccactac
     5941 ttcaagaact ctgtagcacc gtttgtgcca tcatcgctct gctaatccgg ttaccagtgg
     6001 ctgctgccag tggcgttaag gcgtgcctta ccgggttgga ctcaagacga tagttaccgg
     6061 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     6121 cgacctacac cgaactgaga taccaacagc gtgagctatg agaaagcgcc acgcttcccg
     6181 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     6241 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     6301 gacttgagcg tctatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcct
     6361 gctacgtggc cttcttcctg ttcttggtct tttgctcaca tgttctttcc ggccttatcc
     6421 cctgattctg tggataactg tgttaccgtt tttgtgtgag tcagtaccgc tcgccgcagt
     6481 cgaacgaccg agcgtagcga gtcagtgagc gaggaagcgg aaaagcgcct ggacgtgcat
     6541 tttctcctta cgcatctgtg cggcatttca cacccggcat ggcgtacttt tcatacaatc
     6601 cgcactgatg ccgcatggtt aagccagtat acactccgct atcgctacgt gactgggtca
     6661 gggctgcgc
//
