LOCUS       NZ_AP021982             2760 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella sp. WP3-W18-ESBL-02 plasmid pWP3-W18-ESBL-02_10, complete
            sequence.
ACCESSION   NZ_AP021982
VERSION     NZ_AP021982.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194408
            Sequence Read Archive: DRR199244
            Assembly: GCF_014168815.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella sp. WP3-W18-ESBL-02
  ORGANISM  Klebsiella sp. WP3-W18-ESBL-02
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Klebsiella sp. WP3-W18-ESBL-02 isolated
            from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 193x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 08:52:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,417
            Genes (coding)                    :: 5,295
            CDSs (with protein)               :: 5,295
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 28 of 122
            Pseudo Genes (incomplete)         :: 91 of 122
            Pseudo Genes (internal stop)      :: 14 of 122
            Pseudo Genes (multiple problems)  :: 10 of 122
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP021982.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Klebsiella sp. WP3-W18-ESBL-02"
                     /mol_type="genomic DNA"
                     /strain="WP3-W18-ESBL-02"
                     /isolation_source="wastewater"
                     /db_xref="taxon:2675710"
                     /plasmid="pWP3-W18-ESBL-02_10"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6684 N 139.4278 E"
                     /collection_date="2018-02-13"
     gene            complement(101..301)
                     /locus_tag="H7R56_RS27540"
                     /old_locus_tag="WP3W18E02_P100010"
     CDS             complement(101..301)
                     /locus_tag="H7R56_RS27540"
                     /old_locus_tag="WP3W18E02_P100010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_182928891.1"
                     /translation="MTDDRILQLRLIGFRKAETSLRLEGMDPSGKSLYESVKARILSGE
                     LTFDEGRAEIRAHYQKREKSN"
                     /name="antitoxin VbhA family protein"
     gene            complement(1133..1471)
                     /locus_tag="H7R56_RS27545"
     CDS             complement(1133..1471)
                     /locus_tag="H7R56_RS27545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011977836.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="WP_182928892.1"
                     /translation="MTQYCPARGDVILLDFNPQSGHEQAGKRPALVISDDMFNQVTGFA
                     VVCPITNQSKNYPFEVPVVGAKKTTGVILADQFKSLDWKSRQAHTVDAVSAATVESVVS
                     LVSKIIKA"
                     /name="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            complement(1468..1698)
                     /locus_tag="H7R56_RS27550"
                     /old_locus_tag="WP3W18E02_P100020"
     CDS             complement(1468..1698)
                     /locus_tag="H7R56_RS27550"
                     /old_locus_tag="WP3W18E02_P100020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011977835.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="WP_064138467.1"
                     /translation="MTTAIGKWGNGAGLRIPQPFMKQLGLSIGDEVKFHVTEKKLVITK
                     SGPTLEELLGQCNPENRHQEYFCESQGKEML"
                     /name="AbrB/MazE/SpoVT family DNA-binding domain-containing
                     protein"
     gene            1898..2320
                     /locus_tag="H7R56_RS27555"
     CDS             1898..2320
                     /locus_tag="H7R56_RS27555"
                     /inference="COORDINATES: protein motif:HMM:NF025107.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_227674764.1"
                     /translation="MISNDKKIRRESIPMILAGRWGFTALENYGWTTIPNSLLYNQKKL
                     GLDNTELLLLIHLISFIHYGDMPAFPAISTLADRINQHPRTVQRTINKLLKKKIITRKI
                     RSKSSGDKGLSNVYGIEPLIDILANLQDAPSSTNLP"
                     /name="helix-turn-helix domain-containing protein"
     Typing          353..947
                     /name="rep_cluster_2324"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.14"
                     /coverage="92.0"
                     /evalue="0.0"
ORIGIN
        1 gcaacaaaag ggtgtcggcg gtactccaaa aacacatctt gtcccgctgt tttgtggcgc
       61 cgacgcagcg gcggtgacca caaaacattt cctgtccacg tcagttactt ttctcacgct
      121 tctggtagtg cgcccgtatc tcagcacgac cttcgtcgaa ggtcagctca ccggaaagaa
      181 tccgcgcctt gacggactca taaagcgact ttcctgacgg gtccatccct tcaagtctca
      241 gagaagtctc agccttgcgg aaaccgatga ggcgaagttg cagtatccga tcatcagtca
      301 tggattttcc tttgagcagt catgcgaata aggcaggcgt aaagttagct aactttacat
      361 gtcctgcccg gatgttgccg gtgtatagct gtgcaggcaa aagtggaaaa cgacaggaaa
      421 ccgtggcagc agagtgtgta atcagggagg aaacaggcgt accgagcgat acaagtcctg
      481 ttcagcatta cagacattac gcttcctcgg tcattcgctc acttcgttcg gtcatgactc
      541 tgcggcgagc gttactagct catacagaaa gccatgtaga aagtctgggg gattgccggt
      601 tttccaaagg cttcgccctc ctgacaagct tcacagaaac caggtgattt ccggtgctca
      661 aatcagaagt ggcgaaaccc gacaggactt aaagatcccc accgtttccg atgagtcgct
      721 ccctcgtgcg ctctcctgtt cccaccgtga ggtttacccg ttgcagaccg ccgtaaggtg
      781 gcgttttctc attccttccc gtgcaactgg gctcctttta ggtggttcgc tccaagctgg
      841 gctgtatgca cgaactcccc gttcaacccg actaccacgc cttcagagca actactctct
      901 tgaatccaat ctctgaagac acgacgaatc gccattggca gtagccaacg gtaaaaactc
      961 tacaagggag gcgataaaat aatcgcaagc gaatatcttg atgcgcatag cgataaaacg
     1021 ctaaactaac atcacaagtt cgggtgactg ttcccttgta gagcagttac cttaaagtgt
     1081 tctgttacca gcagatcact ttagaaaggc cggcatctag ccggcctttt ttttaagctt
     1141 tgattatctt tgaaacaaga gaaactacgc tctcaacagt agcagccgag acagcatcaa
     1201 cggtatgcgc ttgtcttgat ttccagtcca aagacttgaa ctgatctgcc aaaatgacac
     1261 ctgtagtttt tttagcacca actacaggca cctcgaatgg ataatttttt gactgattag
     1321 tgattgggca aacgactgca aagccagtca cctgattaaa catgtcatca gaaatgacaa
     1381 gagcaggcct tttacctgcc tgctcatgac ccgattgagg attgaaatcc aacaaaatca
     1441 catcacctct agccggacaa tattgtgtca cagcatctcc ttaccctgag actcgcaaaa
     1501 atattcctga tggcgatttt ccggattgca ctgtcccagc agttcctcga gcgttgggcc
     1561 agacttagtt attactaatt ttttctccgt gacgtggaat ttaacctcat caccaatact
     1621 aagcccaagc tgcttcatga acggctgagg aatgcgcaaa cccgcaccat taccccactt
     1681 accgatagcc gtagtcataa tacctccaaa gcagatggat aaacaatgtg taaactatgg
     1741 ttgtatttta taggccaacc cacctacatt ataagggtgg cgtacacgcc accctaaaaa
     1801 gggtcactac tagatactaa taagtataat tacttattag tatctagtag tgacgatgac
     1861 tttttctcaa aaaaaaatta aataaacact tatctatatg atatcaaacg ataaaaaaat
     1921 tcgccgtgaa agcataccaa tgattttagc ggggcgctgg ggttttacgg ccttagaaaa
     1981 ctacggatgg acgacaatac ctaactcctt gttatataat caaaaaaaat taggcttgga
     2041 taatactgag ctgcttctac ttatacatct gatttcgttc atccactacg gtgatatgcc
     2101 agcatttcca gctatatcaa cccttgcaga cagaattaac caacatccaa gaacagtaca
     2161 aagaacaata aataagctat tgaaaaaaaa gataataaca agaaagataa ggtcgaagtc
     2221 gagcggtgat aaggggttaa gcaatgtcta tggtatagaa ccactcatcg acattttagc
     2281 aaacctgcaa gatgcgccct caagtactaa tttaccataa catctcttgg ccgcaccccc
     2341 gcagaggccc tcagcgcgtt tctgccgcga gtgactcgtt tccggtactt tcccccggct
     2401 caaaccctga aaaacgcccc tgatgatttc tgcgcatctg catggctatg cacatgaaca
     2461 ggcacaactc catttcatga caaaagcagg ccgaatgttt caaaaaagaa gttgttcaga
     2521 tgcagccagt cccacagatc cgcggctgtc tttaggcagt tgagtatcag ctcgagcaga
     2581 tttagatatt tcatagtttt ctcctaaaca gtcgatgaat tatttcatca cgacgtattt
     2641 agtccgaaaa ttttgagccc ccagaaacga tgaacgggtg cacagtttac tgtgtggccg
     2701 tgagacgttg ggcaagccgc aggcgcggca gaaataccgc aaaggggtcg gatttaacga
//
