LOCUS       NZ_AP021980             4550 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella sp. WP3-W18-ESBL-02 plasmid pWP3-W18-ESBL-02_8, complete
            sequence.
ACCESSION   NZ_AP021980
VERSION     NZ_AP021980.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194408
            Sequence Read Archive: DRR199244
            Assembly: GCF_014168815.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella sp. WP3-W18-ESBL-02
  ORGANISM  Klebsiella sp. WP3-W18-ESBL-02
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Klebsiella sp. WP3-W18-ESBL-02 isolated
            from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4550)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 193x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 08:52:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,417
            Genes (coding)                    :: 5,295
            CDSs (with protein)               :: 5,295
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 28 of 122
            Pseudo Genes (incomplete)         :: 91 of 122
            Pseudo Genes (internal stop)      :: 14 of 122
            Pseudo Genes (multiple problems)  :: 10 of 122
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP021980.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Klebsiella sp. WP3-W18-ESBL-02"
                     /mol_type="genomic DNA"
                     /strain="WP3-W18-ESBL-02"
                     /isolation_source="wastewater"
                     /db_xref="taxon:2675710"
                     /plasmid="pWP3-W18-ESBL-02_8"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6684 N 139.4278 E"
                     /collection_date="2018-02-13"
     gene            complement(101..943)
                     /locus_tag="H7R56_RS27495"
                     /old_locus_tag="WP3W18E02_P80010"
     CDS             complement(101..943)
                     /locus_tag="H7R56_RS27495"
                     /old_locus_tag="WP3W18E02_P80010"
                     /inference="COORDINATES: protein motif:HMM:NF024098.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="WP_182928888.1"
                     /translation="MHIMYIDEAGCPGALPSPTSPVQPIFLISALIIDSSNLYKITKEI
                     LNLKRNIYKPTLKFEERLKISKDELKGSDLKKDLRYSGGKYKYKQAKRRAKRFIDELLK
                     ILEKYNVRILSRIYIKPPGGIFDGLSVYTSAVQCLYADFNNFLSEKDSQGFVIADNRTL
                     PLNEALSHSLFTEKNKLSGDKYPLVCEMPTFGQSHNHIMLQMTDIVSSALVMPISSHSY
                     CTGHVFSDHVNADNLTIKNDYANKIKSLFYRYMKDGRMKGGLTVNDKIMKKSSSHFFK"
                     /name="DUF3800 domain-containing protein"
     gene            complement(1333..1416)
                     /locus_tag="H7R56_RS27985"
     CDS             complement(1333..1416)
                     /locus_tag="H7R56_RS27985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_320108824.1"
                     /translation="MHEPPVQSDRCVLSGNYRLESNPERHD"
                     /name="replication initiation protein"
     gene            2050..2373
                     /locus_tag="H7R56_RS27500"
                     /old_locus_tag="WP3W18E02_P80020"
     CDS             2050..2373
                     /locus_tag="H7R56_RS27500"
                     /old_locus_tag="WP3W18E02_P80020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_024191642.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQICLDEKPSRAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGTGHDRVQVVAVLMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2582..>3016
                     /locus_tag="H7R56_RS27905"
                     /pseudo=""
     CDS             2582..>3016
                     /locus_tag="H7R56_RS27905"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3051..3536
                     /locus_tag="H7R56_RS27510"
                     /old_locus_tag="WP3W18E02_P80030"
     CDS             3051..3536
                     /locus_tag="H7R56_RS27510"
                     /old_locus_tag="WP3W18E02_P80030"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_061390563.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMISQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTTIQEQKSTQAMLSEKNNGVQLTTCGEERRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            3540..3755
                     /locus_tag="H7R56_RS27515"
                     /old_locus_tag="WP3W18E02_P80040"
     CDS             3540..3755
                     /locus_tag="H7R56_RS27515"
                     /old_locus_tag="WP3W18E02_P80040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_072048928.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSQG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            3884..4411
                     /locus_tag="H7R56_RS27520"
                     /old_locus_tag="WP3W18E02_P80050"
     CDS             3884..4411
                     /locus_tag="H7R56_RS27520"
                     /old_locus_tag="WP3W18E02_P80050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004883515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016246513.1"
                     /translation="MKCFWACSVSVVLAMSFTVAAAENNDNVTYTKPSAGRDLYLTCKG
                     YQVKGASVINVEKPSYLEGTGEQVEFAIITPVGASDGLKVFFDSWGFSSFGLIESHVMH
                     EHWSLVHVGGRASVKGEKIDYSFVDNQPQEGGSYKPKTGVFLYGCMNSDKRPSWVEKIY
                     TRTGVFRGGIED"
                     /name="hypothetical protein"
     Typing          1760..1937
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.222"
                     /coverage="83.0"
                     /evalue="4.5e-50"
     Typing          complement(1308..1777)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.565"
                     /coverage="92.0"
                     /evalue="2.77e-160"
     Typing          2364..3847
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.919"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gcagaacggt gctgaggctg tctgcggcgg tgcgaacaag agatgttatg gtaaatattt
       61 tataagagca cacctattgg agtttaggcg tgctcttatt ctatttaaag aagtgggatg
      121 agcttttttt cattattttg tcattcactg ttagcccgcc tttcattctt ccatctttca
      181 tatatctata aaaaagactt ttaattttat ttgcgtagtc gttttttatt gttaggttat
      241 ctgcattaac atgatcactg aaaacatgac ctgtgcagta actatgagat gaaattggca
      301 taaccaatgc tgaagaaact atatctgtca tctgaagcat tatgtggttg tggctttgac
      361 cgaatgtagg catctcacag actaatggat atttatcccc ggatagttta ttcttctctg
      421 tgaacaaaga gtgtgaaaga gcctcgttaa gcggcagggt tctgttgtca gctatgacaa
      481 agccctggct gtctttttca gatagaaaat tattaaagtc agcgtataag cactgaacgg
      541 ctgatgtata gacggatagg ccatcgaaaa tgccacctgg cggttttata tatatgcgag
      601 acaaaatcct tacattatat ttttctagga tctttaaaag ttcatcaata aatctttttg
      661 cccttctttt tgcttgtttg tatttgtatt tgcctcctga gtatctcagg tcttttttta
      721 aatcactgcc ctttaattca tcctttgata tctttagcct ttcttcaaac ttaagcgttg
      781 gcttgtaaat attccttttt aggttgagaa tttctttggt tattttataa aggtttgatg
      841 agtcaatgat tagtgcggat attaaaaaga ttggttggac ggggcttgtt ggactgggta
      901 gtgcacccgg gcatccagcc tcatctatgt acatgatgtg cattctattc tccataaaca
      961 actaaagccg cataggcggc ttcaggaaac gtcgccttga agttgccccc aagttcgact
     1021 agaaactaac gtttctgatt aaattatata tgacatgagg attgatgcaa gcatttcagt
     1081 ctcattaaaa ttatattgag ttcattggct tcagcgcgta atctcatgct ctgtaaacga
     1141 aaaaaccacc ctggcaggtg gtttttcgaa ggttagttaa tcctggcaga ttttctaacc
     1201 gtggtaacag tcttgtgcga gacatgtcac caaatctgtc cttccagtgt agccgtagta
     1261 aggccaccac ttcaagaact ctcgatacat ctctcgcaca ccctgtttac caatggccgt
     1321 tgccaatggc gattagtcgt gtctttccgg gttggactca agacgatagt taccggacaa
     1381 gacgcagcgg tcggactgaa cggggggttc gtgcatacag tccagcttgg agcgaactgc
     1441 ctacccggaa ctgagtgtca ggcgtggaat gagacaaacg cggccataac agcggaatga
     1501 caccggcaaa ccgaaaggca ggaacaggag agcgcacgag ggagctacca gggggaaacg
     1561 cctgggatct ttatagtcct gtcgggtttc gccgccactg atttgagcgt cagatttcgt
     1621 gatgcttgtc aggggggcgg agcctatgga aaaacggcaa cgccgcttcc tttcttgatt
     1681 tgattgctac tagccaactc ctttacttct gagctggtaa cgcccgccgc agtctcacgg
     1741 caggagcata gcgaccgagt gagcgaggag gcgtcatttt atcaatacag gcattttgca
     1801 cttacgcccc gcagccgcat ttcccccttg ccgtatgaag cactttcctg aacccccact
     1861 ccgatctatc atattaagcc agtatacact ccgctagcgc tacgtgactg gttcagggct
     1921 gcgccccgaa acccgctgaa aagtactgcc gcgccctgcg ggcttgccca acaccgctcc
     1981 ggacaggaaa aaatttcccg cccgaaccgg tgttatccgg aggatggttt ggcagaaaag
     2041 cgcaacaaaa tgctcactat gtgggtgaca gaagacgagc accgccgact tctggagcgc
     2101 tgtgagggta aacaactcgc ggcctggatg cgccagatat gcctggacga aaagccctcg
     2161 cgtgctggca aacttccatc catctcgccg gcgctgcttc gtcagcttgc cggcatgggt
     2221 aacaacctca accagattgc ccggcaggtt aacgccggtg gcggtactgg gcacgatcgc
     2281 gtgcaggttg tcgccgtgct gatggccatt gatgccggac tcgagcggct gcggcacgcc
     2341 gtgctggaaa agggggcaga cgatgatcgt taagtttcat cccagaggcc gcggcggtgg
     2401 cggcggtccg gtggattatc tgctggggaa agaccggcag cgcgacggcg ccagcgttct
     2461 gcagggtaag ccggaggaag tgcgggagct tattgacgcc tcgccttacg ccaagaaata
     2521 cacctccggc gtgctgtctt ttgccgaaca ggatttgccg cccggccagc gtgaaaagct
     2581 gatggcgagc tttgagcggg ttctgatgcc cggactcgat aaagaccagt acagcgtact
     2641 gtgggttgag caccgggaca aagggcggct ggagctgaac ttcctgatcc cgaacacgga
     2701 gctgctgacc ggcagacgtc tccagccgta ttacgaccgc gccgaccgtc cgcgcattga
     2761 tgcctggcag accatagtga acggcagact ggggctgcac gacccgaacg cgccggaaaa
     2821 ccggcgggcg ctggttacac cgtccgcgct gccggaagcg aagcaggaag ccgcccaggc
     2881 gattacgcgc ggcctacttt cccttgcctc atccggggag ctgaaaaccc gtcaggacgt
     2941 cactgaggcg ctggaaagtg cgggttttga ggtggtgcgc accacgaaaa gcagcatcag
     3001 cattgccgac cctgacgggg ggcgaaacat ccgactgaag ggagccatct atgaacagtc
     3061 ttttaacgct ggcgaaggac ttagagcaga aatcgaaagc gcagcagcag actaccggcg
     3121 agatgctgaa agccgcattc agcgagcacg agaagtctgt cagagcggaa ctgagcgaaa
     3181 gcgagaagag aatcagcgcc gccatcctcg accacgaccg gaagctgtcc tcagccatga
     3241 gccaacgcac gaaagggatg ctgcgtatga tcagccagac gtggctgacc atagtcctgg
     3301 tctccgcgct actgatcgcc tcgagcgcgg gcattctgtg gtggcagggg caacagatac
     3361 tcgacaacta cacgaccatc caggaacaga agagcacgca ggccatgctt tcagagaaga
     3421 acaacggcgt ccagctcacg acctgcgggg aggagagacg ccgctgcgtg agggtgaacc
     3481 cggaagcggg aagattcgga gaggattcga gctggatgat actggcgggg aaatagcaca
     3541 tgacggaact ggaaaaacag ttgctgagcg cattagagca gctacagcag gactactcga
     3601 aaaggctgga cgagtgggag aacgccttcg cggaatggcg gacgatgtct gggcttatgc
     3661 aacgggagaa cgcggcgctg agcgagcgcg tcacgggctt gagcaggcag gtgcagagtt
     3721 tgagcgatca gctgcgccgg ttgtcgcaag gctgaatggc atcgaggcgc accgggagca
     3781 ggagcgcgcg gcgcagcacc agaaagcgct ggagctggaa cgatcgcagc ggcagaaagg
     3841 ttttgagctt ggaatgtagt taatcaatga aaagggatgc gttatgaaat gtttctgggc
     3901 ttgctcagtc tcggtggttt tggctatgtc atttacagtc gctgctgcgg agaataacga
     3961 caacgttaca tatacaaaac cgtcagccgg gagggactta tatcttacct gtaaaggtta
     4021 tcaggtgaag ggggcatctg taattaacgt cgagaaacct tcttaccttg aaggtactgg
     4081 tgagcaggtt gaatttgcaa tcattactcc tgttggtgcg tctgacggac tcaaggtctt
     4141 ttttgattca tggggttttt cttcatttgg attgattgaa agccatgtga tgcatgagca
     4201 ctggtcttta gttcatgttg gggggcgtgc ttcagtcaaa ggtgaaaaga ttgattattc
     4261 atttgtagat aaccagccac aggagggggg gagctataaa cccaaaacag gtgtgttcct
     4321 gtacggctgc atgaactcgg ataaaagacc atcttgggtc gaaaagatct acacaagaac
     4381 aggtgtgttt cgagggggca tagaagacta aaatactgct tacagcatga aaatccattg
     4441 aacagagtgt cagttcattt ccgtgcataa ctatgcatac cgtaagaact tacggggggc
     4501 atttatcggc gtttttacgt ggtaagaggt gctcttttgc ggttcatggc
//
