LOCUS       NZ_AP021979             4667 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella sp. WP3-W18-ESBL-02 plasmid pWP3-W18-ESBL-02_7, complete
            sequence.
ACCESSION   NZ_AP021979
VERSION     NZ_AP021979.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194408
            Sequence Read Archive: DRR199244
            Assembly: GCF_014168815.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella sp. WP3-W18-ESBL-02
  ORGANISM  Klebsiella sp. WP3-W18-ESBL-02
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Klebsiella sp. WP3-W18-ESBL-02 isolated
            from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 193x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 08:52:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,417
            Genes (coding)                    :: 5,295
            CDSs (with protein)               :: 5,295
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 28 of 122
            Pseudo Genes (incomplete)         :: 91 of 122
            Pseudo Genes (internal stop)      :: 14 of 122
            Pseudo Genes (multiple problems)  :: 10 of 122
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP021979.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Klebsiella sp. WP3-W18-ESBL-02"
                     /mol_type="genomic DNA"
                     /strain="WP3-W18-ESBL-02"
                     /isolation_source="wastewater"
                     /db_xref="taxon:2675710"
                     /plasmid="pWP3-W18-ESBL-02_7"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6684 N 139.4278 E"
                     /collection_date="2018-02-13"
     gene            complement(208..423)
                     /locus_tag="H7R56_RS27470"
                     /old_locus_tag="WP3W18E02_P70010"
     CDS             complement(208..423)
                     /locus_tag="H7R56_RS27470"
                     /old_locus_tag="WP3W18E02_P70010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015589619.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSSD"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(427..912)
                     /locus_tag="H7R56_RS27475"
                     /old_locus_tag="WP3W18E02_P70020"
     CDS             complement(427..912)
                     /locus_tag="H7R56_RS27475"
                     /old_locus_tag="WP3W18E02_P70020"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_045326458.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVKAELNESA
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVKVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<947..1651)
                     /locus_tag="H7R56_RS27980"
                     /pseudo=""
     CDS             complement(<947..1651)
                     /locus_tag="H7R56_RS27980"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1590..1913)
                     /locus_tag="H7R56_RS27485"
                     /old_locus_tag="WP3W18E02_P70030"
     CDS             complement(1590..1913)
                     /locus_tag="H7R56_RS27485"
                     /old_locus_tag="WP3W18E02_P70030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_007772081.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(2973..4529)
                     /locus_tag="H7R56_RS27490"
                     /old_locus_tag="WP3W18E02_P70040"
     CDS             complement(2973..4529)
                     /locus_tag="H7R56_RS27490"
                     /old_locus_tag="WP3W18E02_P70040"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013199864.1"
                     /GO_function="GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]; GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="WP_045326453.1"
                     /translation="MRIKKPHLRPIVITLSVGGILLTSIFLIAVIMVFQRENIEKDLLD
                     ANSSYAMKMSDVMSGYIEMAQGELAYGAKKIKSTNDIDSLKDEADRLRLQSGMFNSVFV
                     VSNDAVVLATSPESLDLVGTRLNSSASKLAIEEKKPFISSPFKSVTGNLIILLSHPIYD
                     KNGNYIGHVGGSIYLKKHSLFSDVLSRHFFKSDTEISIVSDDGTVIFNNDNSAVGRPMV
                     MSDDLKSKLTESDRGEGEFFFSGNRYLLGYAHMKNTAWNVLVYSHADSATTILITYAKS
                     VLVLLIVIIITFSLLSYFIASRISLPLEKLAISTNAKDIGASLVYIKAINAWYAEADRL
                     KKALFTHVKVMMERVDSLNEVALKDPLTGVNNRLGFSTKIQNNKYGAGASVIAIDVDFF
                     KKINDSFGHGVGDEVLVSLAQIIKSCCRSEDIICRFGGEEFVVFLPNTSVVTAECVAER
                     IRSVIERTVFPNNLRVTVSAGVATQDDPLGGIDFLLKNADDALYQAKREGRNKVIVYSA
                     I"
                     /name="sensor domain-containing diguanylate cyclase"
     Typing          complement(2027..2199)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.126"
                     /coverage="100.0"
                     /evalue="1.99e-77"
     Typing          2124..2676
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.639"
                     /coverage="91.0"
                     /evalue="0.0"
     Typing          complement(105..1600)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.182"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1597..1913)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.396"
                     /coverage="99.0"
                     /evalue="2.47e-61"
ORIGIN
        1 gcgccagata aatttcaccc ggctgcatgg ctatgcagtc gggtgaatgg ctccggcttt
       61 cagcgcagat gtccaccgaa agccccgttt tatcaggtta tcacagtgaa gggctgttat
      121 acgtccgctg tcgctgagat cgctccagct ccagcgcttt ctgatgctgc accacgcgct
      181 cctgctcccg gtgcagctcg acggcattca gtcgctcgac aaccggcgca gctgctcgct
      241 caaactctgc acctgcctgc tcaagcccgt gacgcgctcg ctcagcgctg cgttctcccg
      301 ttgcataaga ccagacatcg tccgccattc cgcgaaggcg ctctcccact cgtccagcct
      361 tttcgagtag tcctgctgta gctgctctaa tgcgctcagc aactgttttt ccagctccgt
      421 catgtgctat ttccccgcca gtatcatcca gctcgaatcc tctccgaacc gtcccgcttc
      481 cgggttcacc ttcacgcagc ggcgtctctg ctcgccgcag gtcgagagct gcacgccgct
      541 gttcctctct gacagcatgg cctgcgtgct cttctgctcc cggatggtcg tgtagttgtc
      601 gagtatctgc tgcccctgcc accacagaat gcccgcgctc gaggcgatca gcagcgcgga
      661 gaccaggacg atggtcagcc acgtctggct gaccatgcgc accatgcctt tcgtgcgctg
      721 gctcatggct gaggacagtt tccggtcgtg gtcgaggatg gcggcgctga ttctcttcgc
      781 gctttcgttc agttccgctt ttacagattt ctcgtgctcg ctgaatgcgg ctttcagcat
      841 ctcgccggta ctctgctgct gcgctttcga tttctgctct aagtccttcg ccagcgttaa
      901 aagactgttc ataaatggct cccttcagtc ggatgtttcg ccccccgtcc gggtcggcaa
      961 tgctgatact gctttttgtg gtgcggacca cctcaaaacc tgcgctttcc agcgcctcag
     1021 tgacatcctg acgggttttc agctccccgg acgaggcaag agcaagcaga ccgcgtgtaa
     1081 tcgcttctgc agcctcctgc ttcgctttag gcagtgcaga cggtgtgacc agtgcccgcc
     1141 ggttctccgg tgcgttcggg tcgtgcagcc ccagtctgcc gttcacgatg gtctgccagg
     1201 catcgatgcg tggacggtcg gcgcgatcgt aatacggctg gagccgtctg ccggtcagca
     1261 gctccgtgtt cgggatcaga aaattcagct caagccgccc cttgtcacga tgctccaccc
     1321 acaggatgct gtactggtct ttatcaagtc cgggcatcag aacccgctca aagcttgcca
     1381 tcagcttttc ccgctggccg gagggtaaat ccgcttccgc aaacgacagt accccggagg
     1441 tgtatttctt aacgtaaggc gacgcgtcaa tcagctcccg gacttcttcc ggcttcccct
     1501 gcaggacggt ggcgccttca cgctgccggt ctttacccag cagatagtcc accggcccgg
     1561 cgccaccgcc gcgaccgcgt ggatgaaatt taacgatcat cgtccgttcc tttctcaaga
     1621 acggcgtgcc gcagccgctc aagtccggca tcgatggcca tcagcgccgc aacaatctgc
     1681 accctgtcat ggccggtacc gccgccggcg ttaacacggc gggctatctg gttcaggtta
     1741 ttgcccatgc cggcaagctg gcgcagcagc gccggcgaga ttgagggaag ctttccggaa
     1801 cgtgccggct tttcgtccag gcaggtctgc cgcatccacg ctgccagctg cctgccgtcg
     1861 cagcgctcaa gcagccgccg gtgctcgtcc tccgtcaccc acatggtgag catcttgttc
     1921 cgtttctctg acaaaccggc ctcctgataa cgccgggacg ggcgggaaag cctttcccgg
     1981 tcggcgcggg gttggacaag ccgcaggcgc gtcaggcttt tggcgggttt cggggcgcag
     2041 ccctgaacca gtcacgtagc gctagcggag tgtatactgg cttactatgt tggcacggat
     2101 gcgagtgtca gtgaaatgct tcatgtggca ggagaaaaat gcagcagcgg agcgtcagca
     2161 gaatatgtga tacaagatat attccgcttc ctcgctcact gactcgctgc gctcggtcgt
     2221 tcggctgcgg cgagcggtac cggcttacag gcggggcgga aatttcctgg gagatgccag
     2281 gaagaagctt aacagggaag cgataaggcc acggcgaagc cgtttttcca taggctccgc
     2341 ccccctgaca agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga
     2401 ctataaagat accaggcgtt tccccctggt ggctccctcg tgcgctctcc tgttcctgcc
     2461 tttcggttta ccggtgtcaa tccgctgtta tggccgcgtt tatctcattc cacgcctgac
     2521 actcggttcc gggaaggcag ttcgctccaa gctggactgt atgcacgaac cccccgttca
     2581 gcccgactac cacgttgtcc cggtaactat catcttgagt ccaacccgga acagacacga
     2641 aaaatcgcca gtggcggtag ccattagtaa gaggattcac ggagcggagt agtagagtgt
     2701 tctacagagt tcttgaagtg gtggcctgac tgcggctaca ctggaaggac agttttggta
     2761 cctgcgctcc gtgaaagcca gttaccttcg gaagaaagag ttggtagctc ttgattccgg
     2821 caaacgaacc accgttggta gcggtggttt ttttgtttgc agggcatgag attacgacga
     2881 catcgaaggg ataacaagag gatccgcttc ttgaaccccc ctccccacaa aaaaaaacgg
     2941 cacactagcg cgtaaagcgt catcaaaaaa actcaaatag cgctatatac aatcacttta
     3001 ttacgcccct ctctcttagc ttgataaaga gcatcgtcag catttttcag caaaaaatcg
     3061 atcccaccaa gaggatcatc ttgtgttgcc acaccagcag atacggtgac acgcaagtta
     3121 ttagggaaaa cagtacgttc aataacactc ctaatacgct ccgcaacaca ctccgctgtt
     3181 accacagatg tattcggcag aaaaacaaca aactcttctc caccaaaccg acaaataata
     3241 tcttcactac ggcaacaaga cttaataatc tgagccaaag aaacaagcac ctcatctccg
     3301 accccatgcc cgaaactatc atttattttt ttaaaaaaat caacatcaat agcaataact
     3361 gaagctcctg ccccatactt gttattctgt attttagttg aaaaccccaa tctattgttc
     3421 acgccggtaa ggggatcttt aagcgccacc tcatttaaag aatcaaccct ttccatcatt
     3481 accttaacat gcgtaaaaag tgccttttta agtcgatcag cttcggcata ccaagcattg
     3541 atagctttaa tataaactaa cgaagcccct atatctttag cattggtaga tatagcaagc
     3601 ttttctaaag gaagtgaaat acgggaggca atgaaatatg aaagaagtga aaatgtaata
     3661 attattacaa ttagcagaac aagtacactt ttagcataag taataagaat agtagtcgcg
     3721 ctgtcagcat ggctataaac caacacattc caagcagtat ttttcatatg agcataaccc
     3781 agaagatatc tattccccga aaaaaaaaat tccccctcac ctctatcaga ctccgttaac
     3841 tttgatttca aatcatcaga cataaccatg ggccgaccaa cagcactgtt atcgttatta
     3901 aagatcacag ttccatcatc actaacaatg ctaatttcag tgtcgctctt gaaaaaatgc
     3961 ctgctcaaaa catcgctaaa taaactatgt ttttttagat atatagaccc accgacatgc
     4021 cctatgtaat tgccattctt atcataaata ggatgtgaca acaaaataat caaattccca
     4081 gtaactgatt tgaacggtga agatatgaat ggtttctttt cctcaatcgc caatttgctg
     4141 gcacttgaat tcaagcgggt accaactaaa tcaagagatt ctggcgaagt cgccagaaca
     4201 acagcatcat tactaacaac aaaaactgaa ttaaacatac cagactgcaa tcttaaccta
     4261 tccgcctcat ctttaagtga gtcaatatca ttagttgatt ttattttttt tgctccatat
     4321 gctaattcac cttgagccat ttcaatataa ccactcatta catctgacat tttcatagcg
     4381 tacgatgaat tagcatcaag caaatctttt tctatatttt cgcgctgaaa aaccataata
     4441 acggcaatca gaaaaataga agttaacaaa atcccaccaa cggacaaggt tatgactatc
     4501 ggacgaaggt gaggcttttt aattctcata aaaatacact ccatataata aaaaatgccg
     4561 catttaccat aacatccctt atcagcaccg ccccggacgg gttcggaaca aatgcggcgt
     4621 cagcccggta aaaccggcag caaactctcc gctaaaccgc gcattct
//
