LOCUS       NZ_AP021976             8285 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella sp. WP3-W18-ESBL-02 plasmid pWP3-W18-ESBL-02_4, complete
            sequence.
ACCESSION   NZ_AP021976
VERSION     NZ_AP021976.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194408
            Sequence Read Archive: DRR199244
            Assembly: GCF_014168815.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella sp. WP3-W18-ESBL-02
  ORGANISM  Klebsiella sp. WP3-W18-ESBL-02
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Klebsiella sp. WP3-W18-ESBL-02 isolated
            from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8285)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 193x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 08:52:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,537
            CDSs (total)                      :: 5,417
            Genes (coding)                    :: 5,295
            CDSs (with protein)               :: 5,295
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 28 of 122
            Pseudo Genes (incomplete)         :: 91 of 122
            Pseudo Genes (internal stop)      :: 14 of 122
            Pseudo Genes (multiple problems)  :: 10 of 122
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP021976.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8285
                     /organism="Klebsiella sp. WP3-W18-ESBL-02"
                     /mol_type="genomic DNA"
                     /strain="WP3-W18-ESBL-02"
                     /isolation_source="wastewater"
                     /db_xref="taxon:2675710"
                     /plasmid="pWP3-W18-ESBL-02_4"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6684 N 139.4278 E"
                     /collection_date="2018-02-13"
     gene            complement(101..940)
                     /locus_tag="H7R56_RS27365"
                     /old_locus_tag="WP3W18E02_P40010"
     CDS             complement(101..940)
                     /locus_tag="H7R56_RS27365"
                     /old_locus_tag="WP3W18E02_P40010"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_182928886.1"
                     /translation="MINFIFISKDDRLLSFAGSEPSLPINESLISNGEKGVISKVKIKN
                     NTKHSDLMGCVLSELGDFYSAYSGVVLFCDSDYYPYLCKHAGLFFMVFDLSSCSADLAD
                     GQLKQKIESLMSLSVKKFMQIKREFLISNGILMRLPYRNFINPLLRGFFDSLKRVHEID
                     IGNLAADIKKIKNDCYKKVPDVNPGRIFVDVNLNNFVFGHEYHSKQGTSSANGHVLLCD
                     VSSKYRFSHRIDEFRHFNVQKRGKKPISGSFYNCHDTRESINKETHINMFTSDFMEY"
                     /name="hypothetical protein"
     gene            complement(947..1342)
                     /locus_tag="H7R56_RS27370"
                     /old_locus_tag="WP3W18E02_P40020"
     CDS             complement(947..1342)
                     /locus_tag="H7R56_RS27370"
                     /old_locus_tag="WP3W18E02_P40020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_182928887.1"
                     /translation="MSDIKKKLSVEVRNYWNKKHKPLLFSTIGDKFKEIKSEEGFVNIG
                     GWISENINDLDAFIYSDPNKPEYIGLVPNGEEYEPEEPKLFSTKISKKSQNNERLVLDF
                     LSILNDLSDDDLKRVIIPTDILVRLMK"
                     /name="hypothetical protein"
     gene            complement(1678..1893)
                     /locus_tag="H7R56_RS27375"
                     /old_locus_tag="WP3W18E02_P40030"
     CDS             complement(1678..1893)
                     /locus_tag="H7R56_RS27375"
                     /old_locus_tag="WP3W18E02_P40030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137421.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSQG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1897..2382)
                     /locus_tag="H7R56_RS27380"
                     /old_locus_tag="WP3W18E02_P40040"
     CDS             complement(1897..2382)
                     /locus_tag="H7R56_RS27380"
                     /old_locus_tag="WP3W18E02_P40040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_110606109.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASGAAILWWQSQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<2417..2806)
                     /locus_tag="H7R56_RS27385"
                     /pseudo=""
     CDS             complement(<2417..2806)
                     /locus_tag="H7R56_RS27385"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2782..5679)
                     /locus_tag="H7R56_RS27390"
                     /old_locus_tag="WP3W18E02_P40050"
     CDS             complement(2782..5679)
                     /locus_tag="H7R56_RS27390"
                     /old_locus_tag="WP3W18E02_P40050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /protein_id="WP_001553819.1"
                     /translation="MSRRHIFTERQRAALFDLPTDELSLLKFYTLGDDDLENIRQRRRP
                     ENRIGFALQLCALRYPGRALAPGEMIPREVLSFVGAQLGVPADALLTYATRRQTRQQHM
                     DTLREIYGYKTFTGRGARDLREWTFGQAEDARSNEDLAHRFIVRCRETSTILPAVSTIE
                     RLCADALVAAERRIETRIAENLTADVRDHLDKLLSEMLAGNISRFIWLRNFEVGNNSAA
                     ANRLLDRLEFLRTLNINHSALASIPAHRIARLRRQGERYFTDGLRDITSDRRWAILAVC
                     VVEWEAAIADAIVETHDRIVGKTWREAKRQHDETISGSKATLTDTIRTFTALGASLLEA
                     RSDGTPLEMAVASSVAWDRLAQLVATGTQLSNTLADEPLAYVGQGYHRFRRYAPRMLRC
                     LKLEAAPVAGPLVAAALSIGEMKGVASPERRFLRPSSKWNRHLRAQEKGDTRLWEVAVL
                     FHLRDAFRSGDVWLAHSRRYGDLKQVLVPMIAAQENAKLAVPSNPQDWLADRKARLTIA
                     LKRLARAARNGTIPHGSIEDGTLRIDRLTADVPDGAEALILDLYRRMPSVRITDMLLEV
                     DAALGFTDAFTHLRTGAPCRDRIGLLNVLLAEGLNLGLRKMAEATNTHDYWQLSRLARW
                     HVESEAMNQALAIVVAAQGKLPMSRVWGMGTSASSDGQFFPTARHGEAMNMVNAKYGSV
                     PGLKAYTHVSDQFAPFACQSIPATVSEAPYILDGLLMNEVGRHVREQYADTAGFTDHLF
                     GASSLLGYNLVLRIRDLPSKRLYVFNPDTTPRELRKLVGGKAREDLIVANWPDIFRCAA
                     TMTAGKIRPSQLLRKLASYPRQNNLAVALREVGRIERTLFIIEWILDTDMQRRAQIGLN
                     KGEAHHALKNALRIGRQGEIRDRTTEGQHYRIAGLNLLTAVIIYWNTVHLGHAVTERRN
                     EGLDVPPEFLPHISPLGWAHILLTGEYLWPKEPKA"
                     /name="Tn3-like element Tn5403 family transposase"
     gene            5774..6379
                     /locus_tag="H7R56_RS27395"
                     /old_locus_tag="WP3W18E02_P40060"
     CDS             5774..6379
                     /locus_tag="H7R56_RS27395"
                     /old_locus_tag="WP3W18E02_P40060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000509966.1"
                     /translation="MGHRAAIYCRVSTADQSCERQEFDLRAFAGRAGYDVVGIFKETGS
                     GTKLDRAERKKVLALAQSRQIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMNGM
                     AFDLSSPYGRMLATFLSGIAEFERDLISERVKSGLAVAKARGKRLGRQAGVRPKSDRLL
                     PKVVAMRAEGRSYRWIARELGISKNTVADIVQRHRANA"
                     /name="recombinase family protein"
     gene            complement(<6413..6739)
                     /locus_tag="H7R56_RS27400"
                     /old_locus_tag="WP3W18E02_P40070"
                     /pseudo=""
     CDS             complement(<6413..6739)
                     /locus_tag="H7R56_RS27400"
                     /old_locus_tag="WP3W18E02_P40070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /name="nuclease"
     gene            complement(6729..7052)
                     /locus_tag="H7R56_RS27405"
                     /old_locus_tag="WP3W18E02_P40080"
     CDS             complement(6729..7052)
                     /locus_tag="H7R56_RS27405"
                     /old_locus_tag="WP3W18E02_P40080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_181421366.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSL
                     SPALLRQLAGMGNNLNQIARQVNGGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKEAG
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            7693..7776
                     /locus_tag="H7R56_RS27970"
     CDS             7693..7776
                     /locus_tag="H7R56_RS27970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058655973.1"
                     /translation="MHEPPVQSDRCALSGNYRLESNPERHD"
                     /name="hypothetical protein"
     Typing          complement(7162..7341)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="89.56"
                     /coverage="83.0"
                     /evalue="7.43e-58"
     Typing          7321..7802
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.123"
                     /coverage="93.0"
                     /evalue="2.1e-176"
     Typing          complement(1575..2749)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.602"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6414..6739)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.33"
                     /coverage="97.0"
                     /evalue="5.27e-63"
ORIGIN
        1 tctttgtgct atagtaagac gttcatttta agtactgacc aaaagtaata gggtattttt
       61 acccaaaaaa aggccaagag aaatcttggc cttttttact tcaatactcc atgaaatcac
      121 tcgtaaacat gttaatatga gtctccttat ttatactttc ccttgtgtca tggcagttat
      181 aaaatgatcc agatatgggt ttcttacccc ttttctgcac attaaaatgc ctaaactcat
      241 cgatcctatg actaaaccta tacttagagc ttacatcgca aagtaaaaca tgcccatttg
      301 cagacgacgt accttgcttt gaatgatatt cgtgaccaaa aacgaaatta ttcaagttta
      361 catctacaaa tattctaccg ggatttacat ctggcacttt tttatagcaa tcgtttttta
      421 ttttctttat atcagcagca agattaccta tatcaatctc atgaaccctc tttagagaat
      481 caaagaatcc ccttagcaac ggattgataa aattacgata tggcagcctc atcaaaatac
      541 cattactaat aagaaactct cttttaatct gcatgaattt cttcactgac aaactcatta
      601 aagattcaat tttttgtttt agctgcccgt cagctagatc tgctgaacaa ctactaagat
      661 caaaaaccat gaaaaaaagc ccagcatgct tacaaaggta agggtagtag tctgagtcac
      721 agaacagcac cactccgctg taagctgagt aaaaatcacc caactcagat agtacacacc
      781 ccattagatc actatgtttc gtattatttt ttatttttac tttcgagata actccttttt
      841 ctccgtttga aatcaaagat tcatttatag gcaatgatgg ttcactaccg gcaaatgaca
      901 aaagccggtc atcctttgaa ataaaaataa aattaatcat gagcgattac ttcataagtc
      961 taaccaaaat atccgttggg atgatcactc gcttcaaatc atcatcactt agatcgttaa
     1021 ggattgaaag aaaatccaaa accagccttt cattattctg acttttctta cttatctttg
     1081 tagaaaatag tttaggctct tccggctcgt actcttcccc attaggaact aaaccgatgt
     1141 attctggttt gttggggtca ctataaataa atgcgtcaag atcatttatg ttttcagaaa
     1201 tccaaccacc tatgttaaca aaaccttctt ctgactttat ttctttgaac ttatcgccaa
     1261 ttgtactaaa cagcaaaggt ttgtgttttt tattccaata atttctaact tctacactta
     1321 acttcttctt aatatcactc ataagtcatc cttaaataaa attcatttac cataacatct
     1381 cttggctgca cctgccgatc cgggttgggt gcgtttccgg cgcgaaacgg gcataagtgg
     1441 cgacagacac accggaaaga cgccggaaac gcacgggata aatttacccg ttttgcatag
     1501 ccatgcaaca caatgattac acgcaggttt ggtgctgatt gggtgctata agcccgattt
     1561 tcaccagtta tcacagcgac gggccgtcat aagtacgctg gcgctgcgat cgttccagct
     1621 ccagcgattt ctggtgctgc accgcgcgca cctgctcccg gtgcgcctcg ataccgttca
     1681 gccttgcgac aaccggcgca gctgctcgct caaactctgc acctgcctgc tcaagcccgt
     1741 gacgcgctcg ctcagcgccg cgttctcccg ttgcataaga ccagacatcg tccgccattc
     1801 cgcgaaggcg ttctcccact cgtccagcct tttcgagtag tcctgctgta gctgctctaa
     1861 tgcgctcagc aactgttttt ccagctccgt catgtgctat ttccccgcca gtatcatcca
     1921 gctcgaatcc tctccgaacc gtcccgcttc cgggttcacc ctcacgcagc ggcgtctctg
     1981 ctcgccgcag gtcgagagct gcacgccgct gttccgctct gacagcatgg cctgcgtgct
     2041 cttctgctcc cggatggtcg tgtaattgtc gagtatctgc tgactctgcc accacagaat
     2101 ggccgcgccc gaggcgatca gcagcgcgga caccaggacg atggtcagcc acgtctggct
     2161 gaccatgcgc agcatccctt tcgtgcgctg gctcatggct gaggacagct tccggtcgtg
     2221 gtcgaggatg gcggcgctga ttctcttctc gctttcgctc agttccgctc tgacagactt
     2281 ctcgtgctcg ctgaatgcgg ctttcagcat ctcgccggta ctctgctgct gcgctttcga
     2341 tttctgctct aagtccttcg ccagcgttaa aagactgttc atagatggct cccttcagtc
     2401 ggatgtttcg ccccccgtcc gggtcggcaa tgctgatgct gcttttcgtg gtgcgcacca
     2461 cctcaaaacc tgcgctttcc agcgcctcag tgacgtcctg acgggttttc agctccccgg
     2521 acgaggctaa ggccagtaaa ccccgcgtaa tcgcctgagc ggcttcctgc ttcgtttccg
     2581 gcagcgcgga cggcgtgacc agggcccgcc ggttttccgg cgcgttcggg tcatgcagcc
     2641 ccagtctgcc gttcactatg gtctgccagg catcgatgcg cggacggtcg gcgcggtcgt
     2701 aatacggctg gagacgcctg ccggtcagca gctccgtgtt cgggatcagg ggtcggttcc
     2761 ggctgagggc gaaatgacac cctaagcttt cggttccttg ggccaaagat attcgccagt
     2821 cagtagaatg tgcgcccagc ccaatgggga tatgtgggga agaaattcag ggggaacatc
     2881 caacccttcg ttccgccgct ccgtgacggc atgaccaaga tggacggtat tccagtaaat
     2941 gatcaccgca gtcaataaat tgagcccagc gattcggtag tgctgcccct ctgtcgtgcg
     3001 atcgcgaatt tccccctgcc tcccgatacg gagcgcattt ttgagcgcat ggtgggcctc
     3061 tcccttgtta agaccgatct gagcacgccg ctgcatgtcc gtatccagga tccactcaat
     3121 aatgaaaagg gtccgttcaa tacgaccaac ttcacgaagc gcaactgcaa ggttgttttg
     3181 tcgtgggtaa gaagcgagct tgcgcaggag ttggctgggc ctgattttgc cagcggtcat
     3241 cgtcgcggca caacggaaaa tatcaggcca gttcgcaacg ataagatcct cccgggcttt
     3301 tccacctacc aacttgcgta actccctggg ggtcgtatcg ggattaaata cgtacaaccg
     3361 cttcgatggc agatccctga ttcgcagaac gagattgtag ccgagcaggc tactggctcc
     3421 gaacaaatgg tcggtgaatc ctgctgtatc ggcatactgt tcgcgaacat ggcgaccgac
     3481 ctcgttcatc agtagtccat cgagaatata cggtgcctcg ctcacggtcg ccgggatcga
     3541 ctgacaagcg aatggcgcga actggtcgct tacgtgagta tacgctttga ggccgggaac
     3601 agaaccatat ttggcattga ccatgttcat ggcttcgcca tgccgcgctg tcgggaaaaa
     3661 ctgaccatcg ctcgatgctg acgtgcccat cccccagacg cgtgacatcg gcagtttacc
     3721 ctgcgcggcc accacaattg ccaatgcctg gttcatggct tcgctttcaa catgccagcg
     3781 ggcaaggcgt gagagctgcc agtaatcatg cgtgtttgta gcttccgcca tcttacgcag
     3841 gcccagattg agcccttcag cgagcaggac gttgagcaga ccgatccggt cgcgacatgg
     3901 agccccggtt ctcagatggg taaacgcatc tgtgaaacca agggctgcat caacttcaag
     3961 cagcatgtcg gtaatccgaa cggacggcat tcggcgatac agatccagta tgagtgcctc
     4021 ggcaccatcc ggcacgtctg ctgtcaacct gtcgatccgc aacgttccat cttctatgct
     4081 accgtgcgga atagtgccgt tacgggcagc ccgggccagc cgcttaagag cgatcgtgag
     4141 tcgcgccttt ctgtctgcca gccaatcctg tgggttggaa ggcacggcca gttttgcatt
     4201 ttcctgcgcc gcgatcatcg gcaccagtac ctgcttgagg tcaccatagc ggcgcgaatg
     4261 agcgagccag acatctccgg aacgaaaagc atcccggagg tgaaagagta ccgccacttc
     4321 ccaaagacgg gtatctcctt tttcctgagc tcgtaaatga cggttccatt tggagctggg
     4381 ccgcaggaaa cgcctttctg gcgatgcaac acctttcatc tctccgatcg acaaagctgc
     4441 tgctaccaat ggtccggcga ccggcgcggc ttcgagcttc agacagcgca acatgcgggg
     4501 cgcataacga cgaaagcgat ggtatccctg cccgacatat gcaagaggct catcggctag
     4561 cgtgttgctg agttgagtcc ctgtcgctac cagttgagcg agccggtccc atgcaaccga
     4621 actggcgaca gccatctcca gcggggttcc gtcactgcgg gcctcaagca acgaagctcc
     4681 cagcgcggtg aaggtacgga tcgtatccgt gagtgtggct ttagagccgg aaattgtttc
     4741 gtcatgctgg cgcttcgctt cccgccaggt ttttcctacg atcctgtcat gggtttcgac
     4801 tatggcatca gcaatcgccg cttcccactc cacaacacag acggcaagga tcgcccagcg
     4861 gcggtccgaa gtgatgtcac gcaaaccgtc ggtgaagtag cgttcaccct gccgacgcag
     4921 ccgggcaatg cgatgggcag gtatgctggc caaagcacta tgattgatat tcagggtacg
     4981 cagaaattcg agcctgtcga gcaaacggtt agcagcagcc gagttgttac caacctcgaa
     5041 gttgcgaagc cagatgaaac gactgatatt gccggcgagc atttcactca gaagtttgtc
     5101 caggtgatcg cgaacatccg ctgttaaatt ttccgcaatc cgcgtttcaa tccgccgctc
     5161 agcggcgacc agagcatccg cgcacaagcg ctcgattgtc gatactgcgg gcagaatggt
     5221 ggaagtttcc cgacaccgca caataaaacg atgagcaaga tcctcgtttg atctggcatc
     5281 ttcggcctgg ccgaaagtcc actcacgcag atcacgggca ccacggcccg tgaaggtctt
     5341 gtagccgtaa atttcgcgca gcgtgtccat gtgctgctga cgggtttggc gccgtgtggc
     5401 ataagtgaga agcgcatcag ccggaactcc aagctgagca ccgacgaagg aaaggacttc
     5461 acgcgggatc atctcaccag gagccagtgc acggcccgga tatcgtaagg cacaaagttg
     5521 cagggcaaag ccaatcctgt tttccggtct gcggcgctgc ctaatgtttt ccaggtcatc
     5581 atcgcccagc gtgtagaact tcagtagcga cagttcgtcc gtgggcagat cgaacagcgc
     5641 tgctcgctgc cgttcggtga aaatatggcg tcgtgacata caaattcgtc ccttttgaag
     5701 tatagtctgt tttggacaac agccagccca tataaatcag ggcgttccga tacaaaaatc
     5761 caggagggtt caattgggac atcgtgccgc catttactgc cgggtttcaa cagcggatca
     5821 gtcttgtgaa cgccaggaat ttgatctgcg agccttcgcc ggccgtgccg gctacgacgt
     5881 ggtgggaata tttaaggaaa caggttcagg aactaaactc gaccgggccg agcgaaagaa
     5941 agtcctggcg cttgcccagt ccagacaaat tgatgcaatc ctggtcactg agctttcccg
     6001 gtgggggcgc tcgacgctcg atctgctcaa tacgctacgt gaactggaga actggaaggt
     6061 ttccgtgata gccatgaatg gaatggcgtt cgatctttcg tcgccgtatg gacgaatgct
     6121 ggcgacgttt ctttccggca ttgcggagtt tgagcgggat ctcatcagcg agcgggtcaa
     6181 gtcaggcctt gctgttgcga aggcacgtgg taagaggctt ggtcgtcagg ccggagtgcg
     6241 accaaaatca gaccgacttt tgcctaaggt ggttgcgatg agggccgagg gacgcagcta
     6301 tcgctggatc gcacgcgagc tcggtatcag caagaatacc gtcgctgaca tcgtgcaacg
     6361 acacagagct aacgcttagg gtgtcatttc gccctcagcc ggaaccgacc ccgatcagga
     6421 agttcagctc cagccgcccc ttgtcctggt gctcaaccca aagcacgctg tactggtctt
     6481 tatcgagtcc gggcatcaga acccgctcga agctcgccat cagcttttca cgctggccgg
     6541 gcggtaagtc ctcttcggca aaggacagga cgccggaggt gtattttttg gcgtagggcg
     6601 aggcgtcgat aagctcccgc acctcttccg gcttcccttg caggacagtg gcgccttcac
     6661 gctgccggtc tttgcccagc agataatcca ccggaccggc gccgccgccg cgcccacgcg
     6721 ggtgaaactt aacgatcatc gcctgcctcc ttttccagta cggcatgccg caatcgctcg
     6781 agtccggcat cgatggccat cagcgcggcg accacctgca cgcggtcgtg cccgctgcca
     6841 ccaccgccgt taacctgccg ggcaatctgg ttaaggttgt tccccatgcc ggcaagctga
     6901 cgaagcagcg ccggcgagag cgacggaagt ttgccggcgc gggcaggctt ctcgtccaga
     6961 cacgtctgcc gcatccaggc tgcgagctgc ttaccgtcgc agcgttccag cagacgccgg
     7021 tgctcatctt ccgttaccca catcgtgagc attttgctgc gtttgtctgc caatccggcc
     7081 tcctgataac gccgggacgg gcgggaaatc ttttcccggt cggcgcggtg ttggacaagc
     7141 cgcaggcgcg tcagcggctt ttagcgggtt tcggggcgca gccctgaacc agtcacgtag
     7201 cgctagcgga gtgtatactg gcttaatatg ttagatcgga gtggtaactc aggaaagtgc
     7261 ttaatgtagc aggagaaaaa tgtggctatg gggcgtaagt gcaatatgca ggtaaggatg
     7321 atatgacgcc tcctcgctca ctcggtcgct gcgctcctgc cgtgagactg cggcgggcgt
     7381 taccggctca cgaatggcgg gataaagaga ctgagtggaa atcaggaaga gcgggaaaag
     7441 gatgcggcgt tgccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga
     7501 cgctcaaatc agtggcggcg aaacccgaca ggactataaa gatcccaggc gtttccccct
     7561 ggtagctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg
     7621 ttatggccgc gttttctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc
     7681 aagctggact gtatgcacga accccccgtt cagtccgacc gctgcgcctt gtccggtaac
     7741 tatcgtcttg agtccaaccc ggaaagacac gactaatcgc cattggcaac ggccactggt
     7801 aaacaggatg tgcgagagat gtatcgagag ttcttgaagt agtggcctgt ctgaggctac
     7861 actggaagga cagatttggt gacatgtctc gcacaagact gttaccacgg ttcgaagagt
     7921 tggtagctct aagaaccttg aaaaaccacc gttggtagcg gtggtttttt cttttacaga
     7981 atcagatact acgtctaacg ctagatagcg gtcaatacgc gcaggattgg tcgcagccta
     8041 aaaagcatgg tattatcact tcactaaaga tttatgtttg gcaagtacaa gaactctccg
     8101 ccttgtattt tgaaggtagg atgttcacga ggtgacggat tttgtctggc aatttagtta
     8161 gccgaaagaa gcacctgaag actgatgtgt agtcagctca caacattcgt taacacttgt
     8221 gttgtgctga gggcaagcat ccataagtcg atgataagat cgagctgtag cattttgaga
     8281 aaata
//
