LOCUS       NZ_AP021962             2694 bp    DNA     linear   CON 05-JAN-2024
DEFINITION  Klebsiella quasipneumoniae strain WP3-W18-CRE-02 plasmid
            pWP3-W18-CRE-02_12 map unlocalized.
ACCESSION   NZ_AP021962
VERSION     NZ_AP021962.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194404
            Sequence Read Archive: DRR199240
            Assembly: GCF_014170655.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Klebsiella quasipneumoniae str.
            WP3-W18-CRE-02 isolated from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 300x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 07:15:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,226
            CDSs (total)                      :: 6,101
            Genes (coding)                    :: 5,865
            CDSs (with protein)               :: 5,865
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 76 of 236
            Pseudo Genes (incomplete)         :: 151 of 236
            Pseudo Genes (internal stop)      :: 39 of 236
            Pseudo Genes (multiple problems)  :: 28 of 236
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP021962.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WP3-W18-CRE-02"
                     /isolation_source="wastewater"
                     /db_xref="taxon:1463165"
                     /map="unlocalized"
                     /plasmid="pWP3-W18-CRE-02_12"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6684 N 139.4278 E"
                     /collection_date="2018-02-13"
     gene            101..706
                     /locus_tag="H7R86_RS30935"
                     /old_locus_tag="WP3W18C02_P120010"
     CDS             101..706
                     /locus_tag="H7R86_RS30935"
                     /old_locus_tag="WP3W18C02_P120010"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_047359292.1"
                     /translation="MSFIKSLNKKNQTDQIEVTINVRLTQEEDHLLTLATQQSGYKRNE
                     LFYLLAKEYIFDDESLKEMAEEPGAEFSEGLYQNCYVVNTNRVHHEEDDKFMISEGVAA
                     TFEDGYKEKMEQVKKGDLVFLYRSGEGIVAHGIASGDVLKKDHNGVPDKTYYQKLSGFV
                     RYEKPLTASEIQRILGRNVVFMHTLFSIRDGYKLLKKD"
                     /name="hypothetical protein"
     gene            1227..1553
                     /locus_tag="H7R86_RS30940"
                     /old_locus_tag="WP3W18C02_P120020"
     CDS             1227..1553
                     /locus_tag="H7R86_RS30940"
                     /old_locus_tag="WP3W18C02_P120020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_228288709.1"
                     /translation="MNQAALITIANELNHDQMRVLMALLAELDYENYIQVAQMDIAEAL
                     TMQKTNVSRAVKNLIDFGIILEGPKIGRSKTYRLNPQFGWKGTVSNHKKALKNGLSVIQ
                     GGRS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1576..2544)
                     /gene="mobV"
                     /locus_tag="H7R86_RS30945"
                     /old_locus_tag="WP3W18C02_P120030"
     CDS             complement(1576..2544)
                     /gene="mobV"
                     /locus_tag="H7R86_RS30945"
                     /old_locus_tag="WP3W18C02_P120030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_047359293.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNADAERTPENYCS
                     VSQSADEAMGKLRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFAHSEQWLE
                     KKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMRDDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYQSINRGTRSQVSISPDALEPRVLRKGIFTKDVEDQAA
                     IAKRLSHAVNDGLAGTIAMASQSAQNAKRVRELQKTMDSQQKRLQSVTEPFKGLSREQM
                     TEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMSR"
                     /name="mobV"
     Typing          complement(1580..2592)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 taaagtcatt gcttaaatca aagatgtgat caatgcttct gtttcaatgt agtgattcac
       61 tatcattgat tagtacatta actctggtga ggaaagtaag atgtcattca taaaatcgct
      121 aaacaaaaaa aatcaaacag accaaataga agtgacgatt aacgttaggt taacccaaga
      181 agaggaccat ttactgacat tagcaaccca gcaatctggt tataaaagga acgagctatt
      241 ctacctccta gcaaaggaat acatctttga tgatgaaagc ttaaaggaaa tggctgagga
      301 gcctggcgct gagttttcag aaggcttata ccaaaattgt tatgtggtta acactaacag
      361 agttcatcat gaagaagatg ataagttcat gatttcagag ggcgtagcgg caacatttga
      421 agatggttat aaggaaaaaa tggagcaggt taaaaaagga gatttagtct ttctttatcg
      481 ctccggcgaa ggaattgttg cacatggtat cgccagtggt gatgttttaa agaaagatca
      541 taatggcgtg ccagacaaga cctactacca gaagctatca ggctttgtta ggtacgaaaa
      601 accactaacc gcgtctgaga tccagagaat actggggaga aacgttgtgt ttatgcacac
      661 gctatttagc attagagatg gctataaact gcttaaaaaa gattaaaccc agtctacagg
      721 tggttggcat cactttgtga caccaaccac acttccggaa gtgtttttta cagtgcatcg
      781 tcaagggtaa atgaactcgc tacgctcgcc cttgacgaaa taaagctccg aatatcgttt
      841 taaaaaatca agaaccgctc gccgcagact catgaccgaa cgtagtgagc gaatgagcga
      901 ggaagcggaa tttccacgtc acttacatag gaaccctaac actcagaaca ggcataaaag
      961 cacctgattt tgccgatgtg tgattgtgga gagtaataag atacagaagg ggatggctgg
     1021 gaagccgcgc cacaagcgga aagtttttga caatagttat ctctagagat aacttagtta
     1081 tcttgagaga taactatttt ttcctgaatg gagattttta gatgcggaga atcacgcagg
     1141 tagaccaaac tacaggggag gaattaggtg gttttgtagc agtgattcgt cctaaacaaa
     1201 agtcatcatt ccagaggcac tttactatga accaagccgc gctaattacc attgccaatg
     1261 agctaaacca tgaccagatg cgtgtgctga tggccttgct cgcagaactg gattacgaga
     1321 actacatcca agttgcgcag atggatattg cggaagcgct gactatgcaa aaaacaaatg
     1381 tcagtcgtgc ggtaaaaaac ctgattgatt tcgggatcat actggaaggt cctaagatag
     1441 gacggagcaa aacttatcgc ttgaatcccc agttcggttg gaaaggtact gtcagcaacc
     1501 acaaaaaagc actaaaaaac ggcctcagcg ttatacaggg cggtagaagc tgatctctgg
     1561 aatgtcgcat caaaactaac gactcatacc acccatgcca cgcgatcgcg tctgtcgctg
     1621 tttttcacgt tcaatacgct gctgtttttc cttttcctgg ttttgctgct taaaccgctg
     1681 agccatcatc aggatttcgg tcatctgctc tctggaaagg cccttaaaag gctctgtaac
     1741 gctctgtagg cgtttctgct gcgagtccat agtcttctgt agctcgcgca ctctttttgc
     1801 attctgcgcg ctctgagagg ccatggcgat ggttcctgca aggccatcgt tcactgcgtg
     1861 acttagacgt ttggctatgg ctgcctgatc ttccacgtct ttggtgaaaa ttcctttccg
     1921 caatacgcgc ggctccagcg catctggcga gattgatacc tggctacggg tgccacggtt
     1981 tatcgattgg tagtagtgct gaactgtctg atgcgtggca cggcttccct cgatgccgcg
     2041 ctcaagaccc aattttttta ctgattcagc gtaggtgctc tgatcgtcac gcatctttga
     2101 ccgcccgcca ataaactctt tcgcggacag ccgtccatcc tgcgtcaatg gcaccacgaa
     2161 cgctgacaaa tgcggggtgg cctcgtcccg gtgaactacc gcagccacaa cacgatcctt
     2221 cccgtatttc ttctccagcc actgctcaga atgggcgaaa aactccgcct gctgccgcgg
     2281 tgtcgcttct ttccaccact cagggcttgc cgtcataacg tactcaacag ccagtaccgc
     2341 atctttacgg cgtttttccg gcaacagctc ccgcagcttt cccatcgcct catctgccga
     2401 ctgagacaca gagcagtagt tttctggtgt ccgttcagcg tcagcattcg gcgtctcacg
     2461 ctccctgtag cagtgctgca aagcgctggc cacactgccc attcccgtca gctttttgca
     2521 gcgcattatc gcgtaattcg ccatgcttac cccctcgttc tggcgtacat ccagccagtc
     2581 gggggatcgc attgcgatgc ctccggcggg ccactatcag gatgatagtg tactcactag
     2641 actttgctac gcaaaatcgt tcgttaaatt caacttaata aaaaatatat gaca
//
