LOCUS       NZ_AP021960             3292 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella quasipneumoniae strain WP3-W18-CRE-02 plasmid
            pWP3-W18-CRE-02_10, complete sequence.
ACCESSION   NZ_AP021960
VERSION     NZ_AP021960.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194404
            Sequence Read Archive: DRR199240
            Assembly: GCF_014170655.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Klebsiella quasipneumoniae str.
            WP3-W18-CRE-02 isolated from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 300x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 07:15:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,226
            CDSs (total)                      :: 6,101
            Genes (coding)                    :: 5,865
            CDSs (with protein)               :: 5,865
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 76 of 236
            Pseudo Genes (incomplete)         :: 151 of 236
            Pseudo Genes (internal stop)      :: 39 of 236
            Pseudo Genes (multiple problems)  :: 28 of 236
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP021960.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WP3-W18-CRE-02"
                     /isolation_source="wastewater"
                     /db_xref="taxon:1463165"
                     /plasmid="pWP3-W18-CRE-02_10"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6684 N 139.4278 E"
                     /collection_date="2018-02-13"
     gene            complement(join(3206..3292,1..171))
                     /locus_tag="H7R86_RS30895"
     CDS             complement(join(3206..3292,1..171))
                     /locus_tag="H7R86_RS30895"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_097734894.1"
                     /translation="MEQAAFLFSGVGGMLLTEMEISNIRNITLIARRLAWINAKRTHID
                     IPSFAELLFSGQKALFFEQYNYFHSRRYTTAGTSKPPDLP"
                     /name="hypothetical protein"
     gene            complement(919..1305)
                     /locus_tag="H7R86_RS30900"
                     /old_locus_tag="WP3W18C02_P100010"
     CDS             complement(919..1305)
                     /locus_tag="H7R86_RS30900"
                     /old_locus_tag="WP3W18C02_P100010"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045282373.1"
                     /translation="MRISNVEWLKKRIAFIRKLGEKTARQRQIIDLLDDEASLSETERR
                     LLHVLATAEKNALQERDAAQKKAVQARISGQKNRRERNHRLFLAAGLMIDAGLVDTKTG
                     ELNFDHEKLLTALKRVRYDLETSA"
                     /name="hypothetical protein"
     gene            1472..2407
                     /locus_tag="H7R86_RS30905"
                     /old_locus_tag="WP3W18C02_P100020"
     CDS             1472..2407
                     /locus_tag="H7R86_RS30905"
                     /old_locus_tag="WP3W18C02_P100020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_045282372.1"
                     /translation="MATYHLSVKFGGKGKALAHASYITREDKFSQRQDLEHTEHGNMPE
                     WARDEPAHFWQAADAFERANGSTYREIEIALPRELNEAQRLALVRDFVKQETGDKHSWT
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERSPEQYFRRYNAKFPARGGARKDSGHNL
                     TPTEQKQALKALRERWERTHNAHMKRYGFEQGFIDSRSLKTQGIDRKPEQHLGFEAAGR
                     LDDSQRQDIKDSRTFSVVDVPEQAKHIDHSDRSWMNASGIEVNRKELVRSLTHDISQQY
                     GREMAARVTKQGNVEIERVKSLSRGFSISR"
                     /name="MobA/MobL family protein"
     gene            2410..2940
                     /locus_tag="H7R86_RS30910"
                     /old_locus_tag="WP3W18C02_P100030"
     CDS             2410..2940
                     /locus_tag="H7R86_RS30910"
                     /old_locus_tag="WP3W18C02_P100030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012634455.1"
                     /translation="MASTEEKLLTLLLSAEHLKEAAKEQQKAVADSLAVARETLKGYRE
                     ISDSVAQQVRDEVRQEVQKMDVAGLIGERISADFQRLEKSVGNMKINADALDKEISIIR
                     SNIIAEYNTLRGYSWKWFAGVAVAFVVLIFATSWFVKTELQDNYNASAAVYQKVADLEK
                     LLTTEQKPEKKKN"
                     /name="hypothetical protein"
     Typing          1473..2404
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.177"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttggccggag aaaagcagtt ctgcaaacga tggtatatcg atatgagttc ttttggcatt
       61 aatccaggca agacgccggg caataagcgt aatattccgg atgttactta tttccatttc
      121 tgtcaacagc atccccccca cgccggagaa taaaaaagcc gcctgttcca tcggcaagcc
      181 ctacatcaag ggaaactacg tcttacgatt cgttttgaac cgggacgctt tgttgctgcg
      241 aatcactaac cagataatcc acgttttgtg agtgggttta ggttaaggct tcaatagaac
      301 aataatagcg ttaagtgatt agggcaacct gtatttgaga gtcgcaaacc catattaggg
      361 caaagaaagc ctaatgcccc cgtggtgggt acaccagaaa acggggagcg aagcgaccag
      421 catcaggccg ttcgtatgct gcttcgattt tgtgggtgtt gcgccagacc ttggcaatca
      481 cgcttcaccg caaccgctgc cttcactctc actttatcag gtacgttatt ggttaacgtg
      541 agaccgggta gagccagctt ctttcccgag ctggtcaggt tcccaccgct tgcctgttta
      601 tcctgccgga gtccggggcg tagacctgag agtcaaggtg cagatatggg tttaatgtcg
      661 ttgaatggta ttaagctggt agtgacattc ctagccagcc ctgctaggat ttgattgtga
      721 cgtctgatcc tggcaggtat ctgcgaagaa ttagagtact caagccccgt gattctgtca
      781 cggggctttt gtacatctgg agcaagaaaa ttcatgctcc tatccgaaaa tccctaccgc
      841 tcgccgcagc cgaacgaccg agcgacgcga gtcagtgagc gaggaagcgg catttctccg
      901 gccttgcaga atgcacgttc acgccgatgt ttccagatcg tagcgtaccc gtttcagcgc
      961 agtcagtaat ttttcgtggt caaaattcaa ctcacccgtt tttgtatcaa ccagtcccgc
     1021 gtcaatcatc agaccagccg ccagaaacag tctgtgattg cgttccctgc ggtttttctg
     1081 ccctgaaatc cgcgcctgaa ccgccttttt ctgtgcggca tcccgttcct gcaacgcgtt
     1141 tttttcagcg gttgccagaa cgtggagcag tcgtctttca gtctcactga gcgaagcttc
     1201 atcatccagc agatcgataa tctgccgttg ccgtgccgtt ttttctccca gctttctgat
     1261 gaaggctatg cgctttttaa gccattcgac gttactgatc cgcatcgttt aacttcccca
     1321 tcccgatatt tccgacgggg aaagtatata acttgcggtc agaagtcgca tgacttatct
     1381 ttactcataa ttctttaaat tagagtaaag cgcacttata tgtttctccc tgcgggcaga
     1441 aacagtgcgc cctgccgggg gctgacacaa catggcaacg tatcatctca gcgtgaaatt
     1501 tggcggcaag ggtaaagccc ttgctcatgc cagttacatt acgcgggaag ataagttcag
     1561 ccagcgccag gatctcgagc acaccgaaca cggcaatatg ccggaatggg cgagagatga
     1621 acccgcccat ttctggcagg ctgcggatgc gttcgaacgt gccaacggtt caacgtacag
     1681 ggaaattgag atcgctctac cccgcgagct gaatgaagcg cagcggctgg cgcttgtccg
     1741 ggattttgtg aagcaggaaa cgggcgacaa acattcatgg acgtttgcga tccacaatcc
     1801 gaaagccagc attgacggcg gcgaacagcc gcacgcccat atcatgatga gccagcgcgt
     1861 caatgacggt attgagcgca gtcccgaaca gtatttcagg cgctataacg ccaaatttcc
     1921 ggcgcgtggc ggcgcgcgca aggactcagg gcataattta acccctacag aacaaaaaca
     1981 ggcactgaaa gccctgcggg agcgctggga gcgtacgcac aacgcgcata tgaaacgata
     2041 tggctttgag cagggtttta ttgatagccg gtcactgaaa acacagggta tcgaccggaa
     2101 accggaacag catctcggtt ttgaagcagc cggacgactg gacgacagcc agaggcagga
     2161 tattaaagac agccggacgt tttcagtcgt ggatgtgcct gaacaggcga aacatatcga
     2221 ccacagcgac cgtagctgga tgaatgccag cggtatcgag gtaaaccgga aggaactggt
     2281 cagatctctg acgcatgata tctcgcagca gtacgggcgg gagatggctg cacgggtcac
     2341 aaaacaggga aatgttgaaa tagagcgcgt gaaatccctg tcacgcggat tttccataag
     2401 caggtaacta tggcgagcac cgaagaaaaa cttctcacac tcctgctgtc ggcggagcat
     2461 ctgaaagagg ccgcaaaaga gcagcagaaa gccgtggcgg attcgctggc ggtggcaagg
     2521 gaaaccctga aaggctaccg ggaaatatcg gacagtgtgg cacagcaggt aagggatgaa
     2581 gtccggcagg aagtgcagaa aatggatgtt gcggggctga ttggcgagcg tatcagcgct
     2641 gattttcagc gtctggaaaa atccgtcggg aacatgaaaa tcaatgcgga cgcgctggac
     2701 aaggaaattt ccattatccg cagtaacatc atcgcggagt acaacacgct gcggggttac
     2761 agctggaagt ggtttgctgg ggttgcggtt gcctttgtcg tactgatatt tgccacaagc
     2821 tggtttgtga aaacggaact acaggacaat tacaacgcgt ctgctgccgt ctatcagaaa
     2881 gtggcagacc ttgaaaagct gctgacaacg gaacagaaac cggaaaagaa aaagaactga
     2941 cgcgctgcgc ttgtccaacg cctcacgttc gcacaataaa ttgcgcaccc gttcaccgtt
     3001 ttcagccgag tttggggcgt cttttgagac ttccggcctg aattcaggcc ggaagttctc
     3061 gccagtcagt caggtgcatg gttatgcatg gggctgaaaa tcacggtatg cgattctgag
     3121 cggttttcgg tgagaagccg ggggttttta tggctcagga cgtaaaaccg ccctgagcgc
     3181 gtttcagcgg tgcgggcaag agatgttatg gtaaatccgg tggctttgac gtgcctgccg
     3241 ttgtgtaccg tcgtgagtga aaataattat attgttcgaa gaacagcgct tt
//
