LOCUS       NZ_AP021959             3447 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella quasipneumoniae strain WP3-W18-CRE-02 plasmid
            pWP3-W18-CRE-02_9, complete sequence.
ACCESSION   NZ_AP021959
VERSION     NZ_AP021959.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194404
            Sequence Read Archive: DRR199240
            Assembly: GCF_014170655.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Klebsiella quasipneumoniae str.
            WP3-W18-CRE-02 isolated from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 300x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 07:15:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,226
            CDSs (total)                      :: 6,101
            Genes (coding)                    :: 5,865
            CDSs (with protein)               :: 5,865
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 76 of 236
            Pseudo Genes (incomplete)         :: 151 of 236
            Pseudo Genes (internal stop)      :: 39 of 236
            Pseudo Genes (multiple problems)  :: 28 of 236
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP021959.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WP3-W18-CRE-02"
                     /isolation_source="wastewater"
                     /db_xref="taxon:1463165"
                     /plasmid="pWP3-W18-CRE-02_9"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6684 N 139.4278 E"
                     /collection_date="2018-02-13"
     gene            517..858
                     /locus_tag="H7R86_RS30870"
                     /old_locus_tag="WP3W18C02_P90010"
     CDS             517..858
                     /locus_tag="H7R86_RS30870"
                     /old_locus_tag="WP3W18C02_P90010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_182980898.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAELMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1067..>1495
                     /locus_tag="H7R86_RS31350"
                     /pseudo=""
     CDS             1067..>1495
                     /locus_tag="H7R86_RS31350"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1536..2033
                     /locus_tag="H7R86_RS30880"
                     /old_locus_tag="WP3W18C02_P90020"
     CDS             1536..2033
                     /locus_tag="H7R86_RS30880"
                     /old_locus_tag="WP3W18C02_P90020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328238.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            2040..2255
                     /locus_tag="H7R86_RS30885"
                     /old_locus_tag="WP3W18C02_P90030"
     CDS             2040..2255
                     /locus_tag="H7R86_RS30885"
                     /old_locus_tag="WP3W18C02_P90030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          259..433
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="83.708"
                     /coverage="81.0"
                     /evalue="2.13e-38"
     Typing          complement(2..152)
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.737"
                     /coverage="84.0"
                     /evalue="3.4e-59"
     Typing          complement(3043..3447)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="82.938"
                     /coverage="94.0"
                     /evalue="1.85e-97"
     Typing          849..2371
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gaacaggaga gcgcacgagg gagctaccag ggggaaacgc ctgggatctt tatagtcctg
       61 tcgggtttcg ccaccactga tttgagcgtc agattttgtg atgcttgtca ggggaggcgg
      121 agcctatgga aaaacggcct cggcgtggcc ttatcgcttc tccgtaaagc ttcttcttgg
      181 catctaccag gaaatttccg ccccgcctgt aagctggtac cgctcgccgc agccgaacga
      241 ccgagcgcag cgagtcagtg agcgaggaag cggcatatca cctgaaccag catcttctcc
      301 tgacgccgga aagcctcatt tcactcctgc cacatgaagc actttggcaa atcaccactt
      361 cggtttaaca tatttagcca gtatacactc cgctagcgct acgtgactgg ttcagggctg
      421 cgccccgaaa ccccactgac gcgcctgcgg cttgtccaac cccgcgccga ccgggaaagg
      481 ctttcccgtc cgtcacggcg ttatcaggag gcgattctgg cagataaacg caacaagatg
      541 ctcacgatgt gggtgacgga agacgaacac cggcggttgc ttgagcggtg cgacggaaaa
      601 caactggcgg cgtggatgcg ccagacctgc cttgatgaga aaccgacacg cgccggcagg
      661 cttccctcga tttcgcccgc gctgctgcgt cagcttgccg gcatgggcaa taacctgaac
      721 cagatcgccc ggaaagtgaa cgccggtggt gccggtcatg accgggtgca gatcgtggca
      781 gagctgatgg cgatcgatgc cggtctggaa cggttgcggc atgccgtact ggaaaaaggg
      841 ccggacgatg attgttaaat tccatccccg gggtcgcggc gggggcgccg gtcctgtgga
      901 ttatcttctg ggtaaagacc ggcagcgtga aggtgccagc gtcctgcagg ggaagccgga
      961 agaagtccgg gagctgattg atgcgtcgcc gtatgtgaaa aaatatacct ccggggtgct
     1021 gtcattcgcg gaggcggacc tgccgccggg tcagcgtgaa aaactgatgg ccagctttga
     1081 gcgggttctg atgcccggac tcgataaaga ccagtacagc attctgtggg tggaacacgc
     1141 agacaagggt cgtcttgagc tgaattttct gatccccaat accgaattac tgacggggaa
     1201 acgcctccag ccgtattacg atcgtgccga ccggccgcgc atcgatgcgt ggcagacagt
     1261 ggtcaacggc aggctggggc tacatgatcc gaacgacccg gagaaccggc gggcgctggt
     1321 cacgccttca ggactgccag aaacgaagca ggaagccgca gagacgatta cacggggttt
     1381 gctgatgtta gcctcatcag gagagctgaa aacgcgtgat gacgtcacag gggcgctcac
     1441 agcggccggt tttgaggtgg tcagaaccac aaaaaacagt atcagcattg ccgacccgga
     1501 cggggggcga aatatccgac tgaggggagc catttatgag cagtctttta acggcctcga
     1561 gggacttcga ggagaaatcg aaagcacagc agcagcttac cgaagagagg ctgaaagccg
     1621 cattcagcaa gcacgagaac gttgtcaaat cggaactgaa cgcaagcgcg aagagaatca
     1681 acgacgccat cagcgcccac gaacagggga tgaacgcggc gatgcagtcg aaccggctga
     1741 gcgtgctgcg aatggtcggg cggacgtggc tgaccatcac catggtgtca gggctgctgt
     1801 tcgccagcct gagcggggtg ctgtggtacc aggggaaccg gatagcggcg aacctggcgg
     1861 aaatcaggca gcagagagat acgctgtcga aactgcacat gcagacgtgg ggcgtgacct
     1921 atctggagaa ccgcaacgga cggtttctgg tgctgccgga ggggatgaaa gccgaaacgg
     1981 gccggacggt ggacaacaag acgcggaacg cggtgaagct ggtacgggag tagagcagaa
     2041 tgacggaact ggaaaaacaa ttgctgagcg cgttagagca gctacagcag gactactcga
     2101 gaaggctgga cgagtgggag agcgccttcg aggaatggcg gaagatgtct ggtcttatgc
     2161 aacgggagaa cgcggcgctg agcgagcgcg tgtcgggctt gagcaggcag gtgcagagtt
     2221 tgagcgagca gctgcgccgg ttatcgacag gctgaatgag attgagcaac agcgatacgg
     2281 gcaaacaacg gaaaaggaac cagagaagac agcagatctg gtaccggaac ggcaacgcac
     2341 attatctcat ggtgacaggg agtatggaga ctgataaacg cccgtctcag gtggcctgag
     2401 cgtgacgggg atcatggcca ttttctgcga tttcagcaca cagagagtct tttttaaccg
     2461 aaatcaccgg ctatgcagtg atgtgaataa gtgatgcata tgcgtaaaat aaatcaggtg
     2521 cgtttctggt ggttttcggt gtacttgcca ccaccttttg cccgtcagcc atcagaaacg
     2581 ccctgagtgc ctccggcgcg gtgcgggtaa gaagggatta tggtaaatag ggcgctatcg
     2641 cctaaatgta cgactgattg ttttttttct ttgtagtatg agattcctga gtagacagct
     2701 acaacaagta gagcaatggc gatagtaagt aaaatccagc ccataaatgc ctgattaatt
     2761 taataaaaac agcctgttat gatttcataa agataatatc atcttttttt agcatgatac
     2821 tgtcgttact tattgtggtg agatgaaaca taaccgctgt ttttgtatgt gtttgttaag
     2881 gcggctaaga ttattcctaa ccactgattt tgtacatatc atcaaaaaaa aataatgccc
     2941 tgtggtgggt atgtaacaaa tcacggggag caggtcaacg ccaaaacttt gaatgatcgg
     3001 gtggcaggct aaatgaaatc ttgagattca ttcagtctcg tcgtaatctc ttgctctgaa
     3061 aacgaaaaaa ccaccttggg aggtggtttt tcgaaggttc agtaagttgg ggaacttctg
     3121 aaccgtggta acagggtgta caagaccgct gccaccaaat acgtcctttc agtttagccg
     3181 tagttaggct tcaacttcaa gaactctgca ccagtaatct cttgtacacc cctttaccag
     3241 tggctaccgc caatggcgat ttgacgtgtc tgtaagggtt ggactcaaga cgatagttac
     3301 cttacatggc gcggtagtcg gactgaacgg gggggtcgtg catacagtcc agcttggagc
     3361 gaactgccta cccggaactg agtgtcaggc gtggaatgag aaaacgcggc cataacagcg
     3421 gaatgacacc ggtaaaccga aaggcag
//
