LOCUS       NZ_AP021957             8604 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella quasipneumoniae strain WP3-W18-CRE-02 plasmid
            pWP3-W18-CRE-02_7, complete sequence.
ACCESSION   NZ_AP021957
VERSION     NZ_AP021957.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194404
            Sequence Read Archive: DRR199240
            Assembly: GCF_014170655.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Klebsiella quasipneumoniae str.
            WP3-W18-CRE-02 isolated from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8604)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 300x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 07:15:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,226
            CDSs (total)                      :: 6,101
            Genes (coding)                    :: 5,865
            CDSs (with protein)               :: 5,865
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 76 of 236
            Pseudo Genes (incomplete)         :: 151 of 236
            Pseudo Genes (internal stop)      :: 39 of 236
            Pseudo Genes (multiple problems)  :: 28 of 236
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP021957.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8604
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WP3-W18-CRE-02"
                     /isolation_source="wastewater"
                     /db_xref="taxon:1463165"
                     /plasmid="pWP3-W18-CRE-02_7"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6684 N 139.4278 E"
                     /collection_date="2018-02-13"
     gene            complement(681..836)
                     /locus_tag="H7R86_RS30785"
                     /old_locus_tag="WP3W18C02_P70010"
     CDS             complement(681..836)
                     /locus_tag="H7R86_RS30785"
                     /old_locus_tag="WP3W18C02_P70010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1508 domain-containing protein"
                     /protein_id="WP_064761103.1"
                     /translation="MNDTWDFYTDGKGEWRWRRTASNGRIVGASSQGYVNRADCIANAR
                     RNGYTG"
                     /name="DUF1508 domain-containing protein"
     gene            1312..2079
                     /locus_tag="H7R86_RS30790"
                     /old_locus_tag="WP3W18C02_P70020"
     CDS             1312..2079
                     /locus_tag="H7R86_RS30790"
                     /old_locus_tag="WP3W18C02_P70020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_053890738.1"
                     /translation="MRFLWRPLPYSRLRECDFLNCIDENDSSSWIAALKIFICIVIKSD
                     LDENGEYCAMITYPQIQKMTGMSRQLICCGFDKLRFYKIISSEGVRKKKHSLLTCEKGS
                     TRQKPLTKRFDGLSTGRWCKLPLKGLIDDDGVIPSFLAMSNRNINDLHALRLFIYLLSV
                     RRHGDVFIVVKAETLMKKLRLKRVQLTLALAHMSALGLIVKERYREGDFLSLNKTPAYA
                     FLLCGWDVLEWNHNRIKESNWRNDYLESFDNWA"
                     /name="hypothetical protein"
     gene            complement(2100..2273)
                     /locus_tag="H7R86_RS30795"
                     /old_locus_tag="WP3W18C02_P70030"
     CDS             complement(2100..2273)
                     /locus_tag="H7R86_RS30795"
                     /old_locus_tag="WP3W18C02_P70030"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_155765196.1"
                     /translation="MDIKTKLKRSLSSVESAISSLKRARGQTTDAYHEISKAIRELEDA
                     EYNIRKSIREME"
                     /name="hypothetical protein"
     gene            3162..3353
                     /locus_tag="H7R86_RS30800"
                     /old_locus_tag="WP3W18C02_P70040"
     CDS             3162..3353
                     /locus_tag="H7R86_RS30800"
                     /old_locus_tag="WP3W18C02_P70040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3353..3775
                     /locus_tag="H7R86_RS30805"
                     /old_locus_tag="WP3W18C02_P70050"
     CDS             3353..3775
                     /locus_tag="H7R86_RS30805"
                     /old_locus_tag="WP3W18C02_P70050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_117097825.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVTDTVKYDVKSDAESTSINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKREPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(4575..>4847)
                     /locus_tag="H7R86_RS30810"
                     /pseudo=""
     CDS             complement(4575..>4847)
                     /locus_tag="H7R86_RS30810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676030.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /name="PH domain-containing protein"
     gene            4888..5358
                     /locus_tag="H7R86_RS30815"
                     /old_locus_tag="WP3W18C02_P70060"
     CDS             4888..5358
                     /locus_tag="H7R86_RS30815"
                     /old_locus_tag="WP3W18C02_P70060"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_136048842.1"
                     /translation="MRMKYFLLATVLASASAIASPETDLKHCVAEANRNSNVNAARDCF
                     ITFTNRFSANTPIANGIVLRNFDHDDGYIIYNFQLTNVDYNTGSEQSLQLLEEIRNWLD
                     SEDATKSQRKGYCKTLGKARDVFEDGAIMRFYDKKGLFITLKVIEASECESH"
                     /name="hypothetical protein"
     gene            <5685..6149
                     /locus_tag="H7R86_RS30820"
                     /old_locus_tag="WP3W18C02_P70070"
                     /pseudo=""
     CDS             <5685..6149
                     /locus_tag="H7R86_RS30820"
                     /old_locus_tag="WP3W18C02_P70070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891053.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA modification GTPase"
                     /name="tRNA modification GTPase"
     gene            complement(6248..6925)
                     /locus_tag="H7R86_RS30825"
                     /old_locus_tag="WP3W18C02_P70080"
     CDS             complement(6248..6925)
                     /locus_tag="H7R86_RS30825"
                     /old_locus_tag="WP3W18C02_P70080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017220146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="WP_182980896.1"
                     /translation="MIKIVSGNIFNTECDYIVNTVNCMGVMGAGIAYEFKLRYPEMYKK
                     YKKICEDNLFDIGMLWIYKDPDKNILCFPTKKHWKYPSKIEYLELGLQKFVSIYKEKGI
                     KSIAFPILGASLGGINIKDSLCIMEKHLSLCDIYIEIWMYDPKSIDDVFINYKKNLLSK
                     EDSYLSEKTGIKINIIKKIKTALMSNDIYSLSGLLSIDGIGEKTLEKSYSFAKKKPDQY
                     DLF"
                     /name="macro domain-containing protein"
     gene            complement(6927..7721)
                     /locus_tag="H7R86_RS30830"
                     /old_locus_tag="WP3W18C02_P70090"
     CDS             complement(6927..7721)
                     /locus_tag="H7R86_RS30830"
                     /old_locus_tag="WP3W18C02_P70090"
                     /inference="COORDINATES: protein motif:HMM:NF025840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4433 domain-containing protein"
                     /protein_id="WP_182980897.1"
                     /translation="MYLIIIFIIIILFFVFPIKNKEQINLKLKDTSDFTKKLFYEINNK
                     NTNSIEETEKTISETVNFGLGNIELNQKKCSCDEIDFDFFGIKYIYHMTHKKNIPNILK
                     YGILSHNEAHYRKIVSNDIADNDVNKRRAKIHDYVPFYFNPKNPMLYKRKDIQDDIVIL
                     CIDRNVLKKECIFTDGNAASSSTIFYDDTRYLNKLNWEIINSRYWGEFSDGKRIRCAEA
                     LIRNKVSQDNILEIYCRNENTCIDLKAETKKIKITINKGIYF"
                     /name="DUF4433 domain-containing protein"
     gene            complement(7732..8103)
                     /locus_tag="H7R86_RS30835"
     CDS             complement(7732..8103)
                     /locus_tag="H7R86_RS30835"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_166488533.1"
                     /translation="MSIFDFFKSKKPKNLLEEFESTALPLVVHGYREIAKRGNIAPTSK
                     TSDQEIINIYQKVGSAFFEAAKKRNERIPALYLNGIVLKFIQIHEMSEEFFYEHLKYEI
                     NNYLANGLRDDYKRELKLF"
                     /name="hypothetical protein"
     gene            8249..>8604
                     /locus_tag="H7R86_RS30840"
                     /pseudo=""
     CDS             8249..>8604
                     /locus_tag="H7R86_RS30840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558268.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     Typing          complement(2649..3114)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.958"
                     /coverage="91.0"
                     /evalue="9.83e-170"
ORIGIN
        1 tctgtaaggt caatccaagt cgtcggtgat tcgtgtacga cttgccgacg actcgtcata
       61 gagtcatcga cgactcactc cacactatca gtaacttatg acgacttgac tctagagtcg
      121 aatcgtcatt ttggtgtttt tttgaccttg gcgactctag agtcaagtca cgagtcgaat
      181 cgccggtgaa tcgtgtggtg agtcgtccgg cgacttgcca taacgtcatt gccggccgtc
      241 gacgaacccg attcctgaac gtgctgaaaa ccggttttcc ggctgcgccg gcaacgcttc
      301 acggccacgc aataaattgc gtccccgttc accgttttcg gatgggaaga acgccggaaa
      361 caggacttac cttggctgag tggcgtgagt tttcgctcta tgtacttgcg tgcatactca
      421 tgcatgccgt aaaaacaaat cctgcgcgtt tctggcgggt tttcgggtgg tttgttgcct
      481 gttttgccgg tttcccgtca gaaacgccct gaggccgttt tagcggtgcg tacaatcagg
      541 gggttatgtt aaatgacaga agtaaaaaaa gcccccgaag aggcttaagg tttttctgta
      601 gggaggaaaa actggtgacc tgtttgacag tacccgttac cgactgacag ccacattcca
      661 gtcgtatacc tgatccaaga ctagccggtg taaccattgc ggcgggcatt agcgatacaa
      721 tccgctcggt ttacatagcc ctgactggat gcaccaacaa tacgaccatt gctggccgtt
      781 ctgcgccagc gccattcgcc tttaccatca gtataaaaat cccatgtatc gttcattctg
      841 tttttatcct gtgctgtgtg gggatcccca agattgatat aaagcatttc tcctcttttt
      901 caatcgataa gagtgtgttt tgcgatgcga gtacgatagg aatttctgtt acatgttacc
      961 catgcaatgc cgtctcatta ggtctaaagg cttcttgtga tgcacataga ctatcttggt
     1021 atcttaatag actatcttta attgttattt tgttgatttt aaagcttttt atatttttgt
     1081 ttattctaga aagattataa taaaaagact tataagtctt tttattatgt gtcatgattt
     1141 tttcaagatt tgagcagagg tgttttcggg aggagagtgt catgggtcta gaaaagtatg
     1201 tgaagttgct cgagagagtt gcagcgattt ctggtgagtt aacccagctt gtaatctctg
     1261 tgacgttatt aataatttct tggtatcttt ttaaataatt aaggtgaagc tatgcggttt
     1321 ttatggaggc ctttgcctta ctctaggtta agagaatgtg attttttaaa ctgtattgat
     1381 gaaaatgatt cgtcttcatg gatagctgca ttgaaaatat tcatttgcat tgttattaaa
     1441 tctgatctgg atgagaatgg agagtattgt gcaatgatta cgtatccaca aatccagaaa
     1501 atgacaggga tgagtagaca gttaatatgt tgtggttttg ataaattgag gttttataaa
     1561 ataatatcta gcgaaggtgt taggaaaaaa aagcattcat tattaacttg tgaaaaaggc
     1621 tctacaagac aaaaaccttt gacgaaaagg tttgatgggc tgtcaacggg gcgatggtgt
     1681 aaactcccgt taaaaggatt gattgatgat gatggtgtaa tcccttcgtt tctggcaatg
     1741 agcaatagaa atattaacga ccttcatgca cttcggttgt ttatctattt gttgtcagtg
     1801 agacggcatg gtgatgtttt tattgttgta aaagcagaaa cgctgatgaa aaaattacga
     1861 cttaaaagag ttcagctaac attggctttg gctcatatgt cagctttggg gttgatagtt
     1921 aaagagcgat atagggaggg cgattttttg agtttaaata aaacacctgc atatgctttt
     1981 ttattgtgtg ggtgggatgt tcttgaatgg aatcacaata gaataaaaga gagcaactgg
     2041 agaaatgatt atttagagtc gtttgataac tgggcttgaa gcccagttat ctatataact
     2101 cactccatct cacgaatgga tttccttatg ttgtactcgg catcctcaag ttcacgaatt
     2161 gctttgctta tttcatggta tgcatccgta gtttgccctc ttgcacgctt taaagatgaa
     2221 attgcacttt ccacagaact taaagaacgt ttaagctttg tcttgatatc catattgaac
     2281 ctctgttttt atctgctgct agggtgattg tgtaacaccc taatggatgg attttattcc
     2341 aataaaatca atgaaataag tctttttctg aggtctattg gtgttgttaa gtggtgctgg
     2401 tttgggtttg atttatgtct atgtatctaa cggatttgct tgtgatcctt tcctcggcgt
     2461 cgtagtctct tgctctgtaa acgaaaaaac cacccggtaa ggtggttttt cgaaggttca
     2521 ttaaccggca agtttatgaa ccgtggtaac agtcttgtgc gagacgtgtc accaaatccg
     2581 tccttccagt gtagcctcag ttaggtcttc acttcaagaa ctcaaataca tctctcgcac
     2641 atcctgttta ccagtggctg ctgccagtgg cgctttgtcg tgtctttccg ggttggactc
     2701 aagatgatag ttaccggata agacgcagca gtcggactga acggggggtt cgtgcataca
     2761 gtccagcttg gagcgaactg cctacccgga actgagtgtc aggcgtggaa tgagacaaac
     2821 gcggccataa cagcggaatg acaccggtaa accgaatggc aggaacagga gagcgcacga
     2881 gggagccatc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccaccact
     2941 gatttgagcg tcaaattctg tgatgcttgt caggggggcg gagcctatgg aaaaacggcc
     3001 gctgtgctgc cttctccctc tccgagctgt ctgctctatg tcgttatgtg cgcgttgcga
     3061 ccgctcgccg cagccgaacg gcaggagcgt agcgactgag tgagcgagga agcggaaaag
     3121 atatctgtct gacattgagc actgacgctc tgaggccctt tatgacgaaa caacagaaaa
     3181 cagcgctgaa catggctaaa ttcatccaga atcagtctct gctcctgctg gagaagctta
     3241 acgagcttga tctcgatgtt gaggccgacc tttgtgagaa actccacgat gatgcggagc
     3301 atcttttccg tacactgtcc tcgagactgg atgaattaca ggatggtaat tgatggccag
     3361 agtgtctatt agtgaagcgg cacggcttgt taaggttagc cgtcccacaa tttacaagat
     3421 gattaacagt gggaagttaa gttacacctc tgttgtaaag cacgggaagg ctattaaagt
     3481 tattgatact tcagagctta ttcgagtgtt tggttctctt gatggtgtaa ccgatactgt
     3541 aaaatatgat gtaaaaagtg acgcagaatc tacaagtatt aacagcgttg gtttacatga
     3601 tttacaacat agaatcgctt tacttgaagc tgaaaacgat ggtcttaaag gggctgttaa
     3661 ggctcgagat gagcatattg attcccttcg tcaggctatg cagcttctgg agcacaagcg
     3721 cgagccttct tcaccacccc attccccgtg gtggaaattc tggaaaaaat cctgacaccg
     3781 tcatgcttca gttttcctct ccggccattc cggggagttc ctgaaccttc ccaaaaatcg
     3841 tcagatctct gaacgcacct caccacttaa aatacccaca caccacgcaa aattaaaatt
     3901 ttgcagataa ctcattaata tcagttagtt ggttgttatt aaattacgca gtattacctt
     3961 ctgtaaggtc aatccaagtc gtcggtgatt cgtgtacgac ttgccgacga ctcgtcatag
     4021 agtcatcgac gactcactcc acactatcag taacttatga cgacttgact ctagagtcga
     4081 atcgtcgctt tggcgttttt atgaccgtgg tgactctaga gtcaagtcac gagtcgaatc
     4141 gccggtgaat cgtgtggtga gtcgtccggc gacttgccat aacgtgattg ccggccgtcg
     4201 acgaacccga ttcctgaacg tgctgacagc gggttttccg gctgcgccgg caacgcttca
     4261 cggccacgca ataaattgcg tccccgttca ccgttttcgg gtaagaagaa catcggaaac
     4321 aggacttacc ccggctgaat ggcgtgaatt ttcgcgctat gcacttgcgt gcatactcat
     4381 gcatgccgta aaaacagagt ctgcgcgttt ctggcgggtt ttcgggtggt ttgttgcctg
     4441 ttttaccggt ttcccgtcag aaacgccctg agggcctctg aggcggtgct gataacggat
     4501 gttatggtaa atccagctga ttggcaggta caaaaaaagc cccgtagggc tttttttgta
     4561 atgctaagag gaagctactg ctgcttagaa ttcatttcct cgatatgatt gttcacaatc
     4621 gatctgaatt gcattggcgc cttgatgttt ggaattgggg tgttggttcc gccagtgcct
     4681 ttaatcacga tagacccata gttaaagatt cgtcccagta tcccttgatg tatgccaagg
     4741 ctttcaactt tatcaatacg gagttccacc gtattgcgtc ggatcaaacc cacttttgcg
     4801 atgactcttt ggttagttaa tgcaagctcc gttgaccaaa ctctaagggc aggtcacagc
     4861 actatgatag ttagaagggg gcttagaatg agaatgaagt attttttact agcaacagta
     4921 ttagcatcgg ccagtgctat tgcttcaccc gaaacagatc tcaagcattg tgtggcagag
     4981 gccaatagaa actcaaatgt caatgcagca agagattgct ttatcacatt cacaaatcgt
     5041 ttttccgcca acacaccaat agctaatggt atcgtcttac gcaactttga ccatgacgat
     5101 ggctacatca tctacaattt ccagttgact aacgtggact acaatactgg gtcggaacaa
     5161 tcattacagc tcttggaaga gataagaaat tggcttgatt ctgaagatgc gaccaaatca
     5221 caaagaaagg gttattgcaa aacacttggg aaagctagag atgtttttga ggatggtgca
     5281 ataatgcgtt tttacgataa aaaaggatta ttcatcactt taaaagtaat tgaggcaagt
     5341 gagtgtgaat cacactagac aaacgggtag tgatgtagcc ccctgaaaca ccggactgta
     5401 gctgtatctc cagataagag ataggcttgg ggtcagtttg gagatcggaa atttttgtac
     5461 gttaagcgca ttatttaacc aatcttggtt cgtaaggggt tgatattgcg tcagtggcta
     5521 tcgcttttaa gttgtcgctc ttgcagtatc gcatttaggt tatccgatta aggtcaaacc
     5581 tctgaaaatg ccgtatagcg cgggagtaca ccctattgct ataggtaagt ctgttcaaaa
     5641 aacaggctta ccgtacaata attctctata tccaaactgc ccccagcaag gtcattcggg
     5701 agctggacag tgcgtccggt aatatgaccg agacggtcag aaagtccgtc agctctgtcc
     5761 tgaatcaggt tgcgctggag ttaaaacaaa ccggcgttga gcagcagaag cccgctgtga
     5821 atgcccttaa tcaggtagtg aaaacggcta atgaatctgt ttacgccatg cgcagtgaaa
     5881 tgtcccgcta tacgtggaaa tctgcgattt atcttgttct gacgatattc ttcgtcatgg
     5941 cttgctgtat gactgcactg acgtggttta tgaatgacgg gtacagccgg atttctgaaa
     6001 tgcagcgtat ggaagcaatc tggcagaaaa aagcgccgct ggctgatatc tcgacatgtg
     6061 acggaaagcc ctgtgttaag gtggacaccc gcactacgta tggtgataaa ggggataaag
     6121 agaatacgtg gatgattatc aaaaaataaa ataacggagc attatgctcc gttatttccg
     6181 cagagacaat attcatttat acatacctat aattaataaa tcttaaccat gcgcatatat
     6241 attttattta aaacagatcg tattgatcag gctttttttt tgcaaatgaa tatgattttt
     6301 ctaatgtttt ttctcctatt ccatcaattg atagtaaacc acttaaactg taaatatcat
     6361 tgctcataag ggctgttttt atttttttta ttatatttat ttttattcct gttttttctg
     6421 aaagatagga gtcttctttt gataagaggt tttttttata attaataaaa acatcatcaa
     6481 ttgattttgg gtcgtacatc caaatctcta tatatatatc acataatgaa aggtgttttt
     6541 ccattatgca cagagagtct ttaatattaa tacctccgag gctggcgcca agaattggga
     6601 atgctataga ttttattccc ttctctttat aaattgatac gaatttttgt aggcctagtt
     6661 ctaaatattc tattttagat gggtatttcc aatgtttctt tgttgggaag cataatatgt
     6721 ttttgtctgg atctttataa atccaaagca tcccaatatc gaaaagatta tcttcgcata
     6781 tttttttgta ctttttatac atctcagggt atcgtagttt gaattcataa gcaattcctg
     6841 cgcccattac acccatgcag ttaactgtat taacaatgta atcacattct gtgttgaata
     6901 tattcccgct gactattttt atcattctaa aaataaattc ctttgttaat agttatcttt
     6961 attttttttg tctcagcttt tagatctatg caagtgtttt catttctgca atatatttcc
     7021 aagatgttat cttgtgatac cttatttctt attagagctt cagcgcatct gattcgtttt
     7081 ccatcagaaa actcccccca gtatcttgag tttattattt cccagtttaa tttatttaaa
     7141 tatcgggtgt catcgtaaaa aattgttgaa gaagatgctg catttccatc cgtaaaaata
     7201 cattcctttt ttaaaacatt tctgtctata catagtatta ctatatcatc ttgaatatct
     7261 ttccttttgt atagcattgg gtttttcggg ttaaaataaa aaggaacata gtcatgtatt
     7321 ttagcccttc ttttattaac atcgttatcg gctatgtcat tacttactat ttttctataa
     7381 tgcgcctcgt tgtgtgaaag tattccatat ttcaatatgt tgggtatgtt ttttttatgt
     7441 gtcatgtgat atatgtactt gatcccaaaa aaatcaaagt cgatttcgtc acatgaacat
     7501 tttttttgat tcagttcaat attgcctaaa ccaaaattga ctgtctcact tattgttttt
     7561 tctgtttctt ctattgagtt cgtgttctta ttatttattt cgtaaaataa tttttttgta
     7621 aaatcacttg tatctttcag ttttaaattg atctgttctt tgttttttat tggaaaaaca
     7681 aagaacagga tgattattat aaaaatgatt attaggtaca tgttaatcca tttaaaaaag
     7741 ttttaattct cttttgtaat catctcttag tccattggct aggtagttat ttatttcata
     7801 ttttaaatgc tcataaaaaa actcttcact catttcatgg atttgtataa atttaagaac
     7861 aattccattt aagtacagag cgggtatcct ttcatttctt ttttttgcag cttcaaaaaa
     7921 tgcagaacct actttttggt atatatttat tatttcttgg tctgatgttt ttgaagtagg
     7981 tgctatattt cctctttttg ctatttctct atatccatga acgacaaggg gcaatgcagt
     8041 tgattcaaat tcttccagta aattttttgg ttttttgctt ttaaaaaaat cgaatatgct
     8101 catgttgttt tccttaatag attgatttgg tagttattat aatcattatg tatttttttt
     8161 catatggttg aagtttattt tttcggtaat gctgccaact tactgattta gtgtatgatg
     8221 gtgattttaa ggtgcttgcg aggcttccat ttccatcaga tgtccttcct gctccgctac
     8281 tgaaggcgtg gtgcgtaacg gcaaaagcac tgccggacat cagcgctatc tctgctctca
     8341 ctgccgtaaa acatggcaga tacagttcac ttacaccgct tctcaacccg gtacgcatca
     8401 gaaaatcatt gatatggcca tgaatggtgt tggatgccgg gcaaccgccc gcattatggg
     8461 cgttggcctc aacacgattt tacgtcactt aaaaaactca ggccgcagtc ggtaacctcg
     8521 cgcatacaac cgggcagtga tgtgattgtc tgcgctgaaa tggacgaaca gtggggctac
     8581 gtcggtgcta aatcacgtca gcgc
//
