LOCUS       NZ_AP021956            10902 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella quasipneumoniae strain WP3-W18-CRE-02 plasmid
            pWP3-W18-CRE-02_6, complete sequence.
ACCESSION   NZ_AP021956
VERSION     NZ_AP021956.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194404
            Sequence Read Archive: DRR199240
            Assembly: GCF_014170655.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Klebsiella quasipneumoniae str.
            WP3-W18-CRE-02 isolated from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10902)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 300x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 07:15:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,226
            CDSs (total)                      :: 6,101
            Genes (coding)                    :: 5,865
            CDSs (with protein)               :: 5,865
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 236
            CDSs (without protein)            :: 236
            Pseudo Genes (ambiguous residues) :: 0 of 236
            Pseudo Genes (frameshifted)       :: 76 of 236
            Pseudo Genes (incomplete)         :: 151 of 236
            Pseudo Genes (internal stop)      :: 39 of 236
            Pseudo Genes (multiple problems)  :: 28 of 236
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP021956.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10902
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WP3-W18-CRE-02"
                     /isolation_source="wastewater"
                     /db_xref="taxon:1463165"
                     /plasmid="pWP3-W18-CRE-02_6"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6684 N 139.4278 E"
                     /collection_date="2018-02-13"
     gene            complement(101..1105)
                     /locus_tag="H7R86_RS30720"
                     /old_locus_tag="WP3W18C02_P60010"
     CDS             complement(101..1105)
                     /locus_tag="H7R86_RS30720"
                     /old_locus_tag="WP3W18C02_P60010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229637.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS4321 family transposase"
                     /protein_id="WP_000427620.1"
                     /translation="MENIALIGIDLGKNSFHIHCQDRRGKAVYRKKFTRPKLIEFLATC
                     PATTIAMEACGGSHFMARKLEELGHFPKLISPQFVRPFVKSNKNDFVDAEAICEAASRP
                     SMRFVQPRTESQQAMRALHRVRESLVQDKVKTTNQMHAFLLEFGISVPRGAAVISRLST
                     LLEDNSLPLYLSQLLLKLQQHYHYLVEQIKDLESQLKRKLDEDEIGQRLLSIPCVGTLT
                     ASTISTEIGDGKQYASSRDFAAATGLVPRQYSTGGRTTLLGISKRGNKKIRTLLVQCAR
                     VFIQKLEHQSGKLADWVRDLLCRKSNFVVTCALANKLARIAWALTARQQTYVA"
                     /name="IS110-like element IS4321 family transposase"
     gene            complement(<1173..3593)
                     /locus_tag="H7R86_RS30725"
                     /old_locus_tag="WP3W18C02_P60020"
                     /pseudo=""
     CDS             complement(<1173..3593)
                     /locus_tag="H7R86_RS30725"
                     /old_locus_tag="WP3W18C02_P60020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612398.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /name="Tn3-like element Tn5403 family transposase"
     gene            3688..4293
                     /locus_tag="H7R86_RS30730"
                     /old_locus_tag="WP3W18C02_P60030"
     CDS             3688..4293
                     /locus_tag="H7R86_RS30730"
                     /old_locus_tag="WP3W18C02_P60030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000509966.1"
                     /translation="MGHRAAIYCRVSTADQSCERQEFDLRAFAGRAGYDVVGIFKETGS
                     GTKLDRAERKKVLALAQSRQIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMNGM
                     AFDLSSPYGRMLATFLSGIAEFERDLISERVKSGLAVAKARGKRLGRQAGVRPKSDRLL
                     PKVVAMRAEGRSYRWIARELGISKNTVADIVQRHRANA"
                     /name="recombinase family protein"
     gene            4362..4487
                     /locus_tag="H7R86_RS31345"
     CDS             4362..4487
                     /locus_tag="H7R86_RS31345"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_266097102.1"
                     /translation="MTIYSDDTRRNTYLVVLPKQAHGVETYTSTTDNTVVKYTAK"
                     /name="hypothetical protein"
     gene            4548..4781
                     /locus_tag="H7R86_RS30740"
                     /old_locus_tag="WP3W18C02_P60040"
     CDS             4548..4781
                     /locus_tag="H7R86_RS30740"
                     /old_locus_tag="WP3W18C02_P60040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_023207133.1"
                     /translation="MTEMEKQLLSALESLQTSYEQQQQAWQDSYSSLQHMFEATSQALT
                     HSDRVCQHLSSQVDSLRAQVESLSRNVSRLMR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            4943..5275
                     /locus_tag="H7R86_RS30745"
                     /old_locus_tag="WP3W18C02_P60050"
     CDS             4943..5275
                     /locus_tag="H7R86_RS30745"
                     /old_locus_tag="WP3W18C02_P60050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_023207134.1"
                     /translation="MSHAIEFIETPMFTRQIKQIATDEELRELQKTLIESPDKGDIIQK
                     TGGLRKVRMATGNQGKSGSARVIYFLATAEVIYLVMAYPKSTKDSLTDAEKAALKTLTQ
                     QLKDEV"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            5277..5561
                     /gene="nadS"
                     /locus_tag="H7R86_RS30750"
                     /old_locus_tag="WP3W18C02_P60060"
     CDS             5277..5561
                     /gene="nadS"
                     /locus_tag="H7R86_RS30750"
                     /old_locus_tag="WP3W18C02_P60060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_001697687.1"
                     /translation="MSFFDELKASLEEAVEIKNGVREPARVTRYELADVKAIRAQLNVS
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFQELAAH"
                     /name="nadS"
     gene            5753..6676
                     /locus_tag="H7R86_RS30755"
                     /old_locus_tag="WP3W18C02_P60070"
     CDS             5753..6676
                     /locus_tag="H7R86_RS30755"
                     /old_locus_tag="WP3W18C02_P60070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_052909835.1"
                     /translation="MHNAALELFNERLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRAGAAIDWSDRNAPAPTLTITNPDNGHAHLLYALKTSIRTAPDGKMK
                     PLRYAAAVENALRKKLEADAGYSGLICKNPNHGHWKIAVWQPELYTLDWLADFLDLNAA
                     NDKEIVADYGLGRNCTLFDRTRKWAYRAIRQGWPEYDQWLQACYERACVYNLQFAAPLD
                     ENEVSAIAKSIAKWTGKNFSKETFSSYVKKTHSSSIQSLRGRIGGSKSRGGGRYIDDFS
                     ERTLEPWLDMGISRATYYRRKKKSGI"
                     /name="replication initiation protein"
     gene            6666..8444
                     /locus_tag="H7R86_RS30760"
                     /old_locus_tag="WP3W18C02_P60080"
     CDS             6666..8444
                     /locus_tag="H7R86_RS30760"
                     /old_locus_tag="WP3W18C02_P60080"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0003677 - DNA binding [Evidence IEA]; GO:0016787 -
                     hydrolase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="WP_024262774.1"
                     /translation="MAFEKKDITAKHKLRRPQTEAFGKIREHYEKKELKEVGLILPVGC
                     GKSGLISITPYATESSRVLIIAPGKKIRDQLAKDMKFNEPDNFYNKCEFFDLVDDYPEV
                     CIIEAGGKTNIHDIRSSDIVVSNIQQIAGDENKWLEQLDADFFDLIIVDEAHHNKASTW
                     VKVKEKFPNAKVINYSATPMRSDGQLMSGEIIYSFPVVEAIREGYIKRLHAKMISPSKL
                     VYVDSDGNEITLTNPEDIKRLGEEEASFRRGVLMSDETLSTLVDLSIKELNRLREESGE
                     KRLKIIASALNYGHCIQIKEAFLARNLRADYVHSNEDGAVNDRVFSKLENHELDVIVQS
                     KMLGEGFDHKFLSVAMVGSVFSNLSPFVQFVGRVMRVVKQNSPGDLVNRGVVVFHVGAN
                     IANRWSDFRNFSQADQDFFADLLPEVEDVDFGSDGTSDRDPDETGGSNQLIPVEVTSTD
                     GVTASELDPIGDFQELKKELASLGMTTNEAIEILRRSRLSKQDQRRAGRQAINDLVNHH
                     AGLLINQLGFKPKGRNFNKSQDNYSWVVRELHNRVNEHIGCNSGDRENLSLEQVDEAKS
                     AVPNLAEDIRQEWSNG"
                     /name="DEAD/DEAH box helicase family protein"
     gene            8437..8670
                     /locus_tag="H7R86_RS30765"
                     /old_locus_tag="WP3W18C02_P60090"
     CDS             8437..8670
                     /locus_tag="H7R86_RS30765"
                     /old_locus_tag="WP3W18C02_P60090"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023207131.1"
                     /translation="MAKFKSLIVGAKFEVASRKTTCKHDKKHVITKGEFRLSVKNSTQG
                     ESYYCLSCAKTMVTESQAKLEGLKSELDSLSL"
                     /name="hypothetical protein"
     gene            8904..9230
                     /locus_tag="H7R86_RS30770"
                     /old_locus_tag="WP3W18C02_P60100"
     CDS             8904..9230
                     /locus_tag="H7R86_RS30770"
                     /old_locus_tag="WP3W18C02_P60100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000415578.1"
                     /translation="MEKRTKEIKIRLTETEHQRLLDRCDRTHLAEWLRQLGLGEHTSRK
                     RRVPDVAPELLRQVSGIGNNLNQIARRLNQTDSLTPSERASLLVVLTSLDRQLGDLLEQ
                     NRDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            9439..>9861
                     /locus_tag="H7R86_RS31275"
                     /pseudo=""
     CDS             9439..>9861
                     /locus_tag="H7R86_RS31275"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            9908..10255
                     /locus_tag="H7R86_RS31280"
     CDS             9908..10255
                     /locus_tag="H7R86_RS31280"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_228288703.1"
                     /translation="MSEILNLAKGFEEKSKQQAQDTEQSVKREFVRLETSISAELQSSS
                     QSISNAIREQNGRLTGMLRKSVSTAMAWIFFCFALLIGIMGGIIWFQGTIITSRLSEMD
                     SYSQQLGVGSG"
                     /name="MbeB family mobilization protein"
     gene            complement(10271..>10735)
                     /locus_tag="H7R86_RS30780"
                     /old_locus_tag="WP3W18C02_P60120"
                     /pseudo=""
     CDS             complement(10271..>10735)
                     /locus_tag="H7R86_RS30780"
                     /old_locus_tag="WP3W18C02_P60120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /name="Tn3 family transposase"
     Typing          4329..4879
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.391"
                     /coverage="100.0"
                     /evalue="1.59e-79"
     Typing          9221..10239
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.176"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttttacaggt tcctcaacag gccggtgggc cgttagtatc atcaatatca gtattcgcaa
       61 aaccagatca gtgattcttt aaaccggtgt atttctgccg ttatgctaca taagtttgct
      121 gtcgtgccgt tagggcccag gctattctgg ccagcttgtt tgccagagca caagtgacga
      181 caaagttgct tttcctacac agtagatccc tgacccaatc ggccaatttg ccagactggt
      241 gttccagttt ttgtatgaat accctggcac attgaaccaa caaagttcgg atctttttgt
      301 tacctcgctt actaattccc agcaatgtcg tccgacctcc cgtgctgtac tgtcgaggca
      361 ctagccctgt tgccgccgca aagtcacgac tgctggcgta ctgcttcccg tcgccaatct
      421 cagttgaaat agtactcgct gtcagtgttc cgacgcaggg aatgctcagc aagcgctgtc
      481 caatctcatc ttcgtccaac tttcgtttca actgggattc caaatcttta atctgctcaa
      541 caagatagtg ataatgctgt tgtaatttca gcaataactg gctgaggtat agaggcaaac
      601 tattgtcctc aagaagggta ctcagtcggc taataacggc agctcctcgt ggaacgctga
      661 tgccaaattc cagcagaaaa gcatgcatct gattggttgt ttttacctta tcctgaacca
      721 gggattcacg gacacgatgc agcgcacgca ttgcctgctg agattcagtt ctgggctgta
      781 caaaacgcat agacggacgc gatgcagctt cacaaatagc ttcggcgtcg acaaagtcgt
      841 ttttgttact tttaacgaat ggacggacaa attgtggtga tatcagctta ggaaaatgcc
      901 ccaactcttc caacttgcgt gccataaagt gagagccacc acaggcttcc attgcgatgg
      961 ttgtagcggg gcatgtcgcc aaaaattcga ttaactttgg ccgtgtaaat tttttacggt
     1021 aaacagcctt gccgcgacga tcctggcaat gaatatggaa agagttttta cccagatcga
     1081 taccaatgag cgcaatgttt tccatgatag ttctccgaat gaaagcctat cctcagcata
     1141 gtaccgggaa ggagggagtg accatctcat taaatatcag gccagttcgc aacgataaga
     1201 tcctcccggg cttttccacc taccaacttg cgtaactccc tgggggtcgt atcgggatta
     1261 aatacgtaca accgcttcga tggcagatcc ctgattcgca gaacgagatt gtagccgagc
     1321 aggctactgg ctccgaacaa atggtcggtg aatcctgctg tatcggcata ctgttcgcga
     1381 acatggcgac cgacctcgtt catcagtagt ccatcgagaa tatacggtgc ctcgctcacg
     1441 gtcgccggga tcgactgaca agcgaatggc gcgaactggt cgcttacgtg agtatacgct
     1501 ttgaggccgg gaacagaacc atatttggca ttgaccatgt tcatgacttc gccatgccgc
     1561 gctgtcggga aaaactgacc atcgctcgat gctgacgtgc ccatccccca gacgcgtgac
     1621 atcggcagtt taccctgcgc ggccaccaca attgccaatg cctggttcat ggcttcgctt
     1681 tcaacatgcc agcgggcaag gcgtgagagc tgccagtaat catgcgtgtt tgtagcttcc
     1741 gccatcttac gcaggcccag attgagccct tcagcgagca ggacgttgag cagaccgatc
     1801 cggtcgcgac atggagcccc ggttctcaga tgggtaaacg catctgtgaa accaagggct
     1861 gcatcaactt caagcagcat gtcggtaatc cgaacggacg gcattcggcg atacagatcc
     1921 agtatgagtg cctcggcacc atccggcacg tctgctgtca acctgtcgat ccgcaacgtt
     1981 ccaacttcta tgctaccgtg cggaatagtg ccgttacggg cagcccgggc cagccgctta
     2041 agagcgatcg tgagtcgcgc ctttctgtct gccagccaat cctgtgggtt ggaaggcacg
     2101 gccagttttg cattttcctg cgccgcgatc atcggcacca gtacctgctt gaggtcacca
     2161 tagcggcgcg aatgagcgag ccagacatct ccggaacgaa aagcatcccg gaggtgaaag
     2221 agtaccgcca cttcccaaag acgggtatct cctttttcct gagctcgtaa atgacggttc
     2281 catttggagc tgggccgcag gaaacgcctt tctggcgatg caacaccttt catctctccg
     2341 atcgacaaag ctgctgctac caatggtccg gcgaccggcg cggcttcgag cttcagacag
     2401 cgcaacatgc ggggcgcata acgacgaaag cgatggtatc cctgcccgac atatgcaaga
     2461 ggctcatcgg ctagcgtgtt gctgagttga gtccctgtcg ctaccagttg agcgagccgg
     2521 tcccatgcaa ccgaactggc gacagccatc tccagcgggg ttccgtcact gcgggcctca
     2581 agcaacgaag ctcccagcgc ggtgaaggta cggatcgtat ccgtgagtgt ggctttagag
     2641 ccggaaattg tttcgtcatg ctggcgcttc gcttcccgcc aggtttttcc tacgatcctg
     2701 tcatgggttt cgactatggc atcagcaatc gccgcttccc actccacaac acagacggca
     2761 aggatcgccc agcggcggtc cgaagtgatg tcacgcaaac cgtcggtgaa gtagcgttca
     2821 ccctgccgac gcagccgggc aatgcgatgg gcaggtatgc tggccaaagc actatgattg
     2881 atattcaggg tacgcagaaa ttcgagcctg tcgagcaaac ggttagcagc agccgagttg
     2941 ttaccaacct cgaagttgcg aagccagatg aaacgactga tattgccggc gagcatttca
     3001 ctcagaagtt tgtccaggtg atcgcgaaca tccgctgtta aattttccgc aatccgcgtt
     3061 tcaatccgcc gctcagcggc gaccagagca tccgcgcaca agcgctcgat tgtcgatact
     3121 gcgggcagaa tggtggaagt ttcccgacac cgcacaataa aacgatgagc aagatcctcg
     3181 tttgatctgg catcttcggc ctggccgaaa gtccactccc gcagatcacg ggcaccacgg
     3241 cccgtgaagg tcttgtagcc gtaaatttcg cgcagcgtgt ccatgtgctg ctgacgggtt
     3301 tggcgccgtg tggcataagt gagaagcgca tcagccggaa ctccaagctg agcaccgacg
     3361 aaggaaagga cttcacgcgg gatcatctca ccaggagcca gtgcacggcc cggatatcgt
     3421 aaggcacaaa gttgcagggc aaagccaatc ctgttttccg gtctgcggcg ctgcctaatg
     3481 ttttccaggt catcatcgcc cagcgtgtag aacttcagta gcgacagttc gtccgtgggc
     3541 agatcgaaca gcgctgctcg ctgccgttcg gtgaaaatat ggcgtcgtga catacaaatt
     3601 cgtccctttt gaagtatagt ctgttttgga caacagccag cccatataaa tcagggcgtt
     3661 ccgatacaaa aatccaggag ggttcaattg ggacatcgtg ccgccattta ctgccgggtt
     3721 tcaacagcgg atcagtcttg tgaacgccag gaatttgatc tgcgagcctt cgccggccgt
     3781 gccggctacg acgtggtggg aatatttaag gaaacaggtt caggaactaa actcgaccgg
     3841 gccgagcgaa agaaagtcct ggcgcttgcc cagtccagac aaattgatgc aatcctggtc
     3901 actgagcttt cccggtgggg gcgctcgacg ctcgatctgc tcaatacgct acgtgaactg
     3961 gagaactgga aggtttccgt gatagccatg aatggaatgg cgttcgatct ttcgtcgccg
     4021 tatggacgaa tgctggcgac gtttctttcc ggcattgcgg agtttgagcg ggatctcatc
     4081 agcgagcggg tcaagtcagg ccttgctgtt gcgaaggcac gtggtaagag gcttggtcgt
     4141 caggccggag tgcgaccaaa atcagaccga cttttgccta aggtggttgc gatgagggcc
     4201 gagggacgca gctatcgctg gatcgcacgc gagctcggta tcagcaagaa taccgtcgct
     4261 gacatcgtgc aacgacacag agctaacgct tagggtgtca tttcgccctc agccggaacc
     4321 gaccccagct ggcgaacctg aaagcgaaaa gcggcgcggg cgtgacgatt tacagcgacg
     4381 acacgcgccg gaacacctac ctggtggtgc tgccgaagca ggcgcacggc gtggaaacct
     4441 acacgtcgac gaccgacaac accgtggtga aatacacagc gaaataaccc ctccagagcg
     4501 ccctgaaacg ccacagcgcg tttttaacac tgaggacgac caaaaccatg accgagatgg
     4561 aaaaacagct tctgagcgcc ttagagagct tacagacaag ctacgagcaa cagcagcagg
     4621 cgtggcagga cagctacagc agtttgcagc acatgttcga ggctacctca caggcgctca
     4681 cgcacagcga ccgggtgtgt cagcacttga gcagtcaggt ggacagcttg cgcgcgcagg
     4741 tcgagagctt gagcaggaac gtcagccgct tgatgcgctg atcaggcagc acgaccgcgc
     4801 ctacgccgag aagctgcgtg agcagcagcc agagccggag ccgcagaaaa aaccgacccg
     4861 ctactatggc ccctcgatgt gaccgctttt ccggaatcag aattaggtgt actatgtact
     4921 ccattaaaaa caggagacag ccgtgagtca cgcgattgag tttatcgaga ccccgatgtt
     4981 tacccgtcag ataaaacaga tcgccacgga tgaagagctc agggagctgc aaaagacgct
     5041 gattgagtcg ccggacaaag gcgatatcat ccagaaaacc ggcgggctgc gaaaagtcag
     5101 gatggcgacg ggcaatcagg gaaaaagcgg cagtgcgagg gtaatttatt ttctggccac
     5161 agccgaggtg atttatctgg tgatggccta cccgaaaagc accaaagaca gcctgacgga
     5221 cgcggagaaa gccgcgctga agacgctgac ccaacaactg aaagacgagg tgtgacatga
     5281 gtttttttga cgagctgaaa gcatcgctgg aagaagccgt tgaaatcaaa aacggcgtca
     5341 gagagcctgc ccgcgtcacc cgttacgagc tggcagacgt gaaggccatc agggcacagc
     5401 tgaacgtttc acagagcgag atggcgaaag tgctgggaac cagcctcgac actatcaaga
     5461 gctgggaaac cggacgccgt aatccgaccg gactggcggc gaaggtgctg gcaaccatcc
     5521 aggcgaatcc gaaattcttc caggaactgg cggcgcacta agccgttgtg acagaaggat
     5581 tcgacatgcg aggccgttcg cgtgtaattt aacggcaaca gaaaaaacaa agccccggaa
     5641 atcatgctca ttcttggcgg agcgcatgat aaccagggcc aggtcgaaac cttgaaagga
     5701 tattagcaca tgaaccgtgc cagacaacag accgccataa gcgcagggcg cattgcataa
     5761 cgctgcactc gaactcttca acgagcgctt accgcacaag ccgtactttt cggacgatct
     5821 gcattttggt gtacggattg ccggtaagga acgcgccatt ctggcgaaat atatccagtt
     5881 caatcagcct catgccatgt tctggctggg attcgacgtt gatcgcgccg gtgccgccat
     5941 cgactggagc gaccgcaacg cgcccgcgcc cacgctgacc attaccaacc ccgacaacgg
     6001 acatgcgcac ctgctgtatg cgctgaaaac ctcgatacgc accgcgccgg acggaaaaat
     6061 gaagcctctg cgctacgctg ccgccgtgga gaacgcgcta cgcaaaaaat tagaggctga
     6121 cgcggggtat tcaggtctaa tctgcaaaaa tccgaaccac ggccactgga aaattgccgt
     6181 gtggcagccg gaactctaca cgctggactg gctggccgac tttctcgact taaacgcagc
     6241 gaatgacaaa gaaatcgtcg ccgactacgg cttaggccgt aactgcaccc tgttcgacag
     6301 aacccggaag tgggcatatc gcgccatccg tcagggctgg cctgagtacg accagtggct
     6361 acaagcctgc tatgaacgtg cctgcgttta taacctgcaa tttgcagcgc ctctggatga
     6421 aaatgaggtt agtgcgatag ctaaaagtat tgcgaagtgg actggtaaaa acttttcaaa
     6481 agaaaccttt tcttcttatg ttaagaaaac tcactcatct tccattcaat cattgagggg
     6541 gcgaattgga ggaagtaaga gtagaggcgg aggaagatat atagatgatt tcagtgaaag
     6601 aactctggaa ccatggttag acatggggat cagccgagct acctactaca gaaggaagaa
     6661 aaaaagtggc atttgaaaaa aaagacatca cagctaaaca caagttaaga agaccacaga
     6721 ccgaggcatt cggtaagata agagaacact acgaaaaaaa agaattgaaa gaagttgggc
     6781 tcatactgcc ggttggttgc gggaagtctg gtcttatatc aataactcca tatgcaacag
     6841 aatctagccg tgttcttata attgcgcctg gtaaaaaaat aagagaccag ttagctaaag
     6901 acatgaagtt taatgagcct gataattttt acaacaagtg cgaatttttt gatttggtag
     6961 atgactatcc agaagtttgc ataattgagg caggtggtaa gactaatata cacgacatta
     7021 ggtcaagtga tattgtcgtt tccaatatcc aacaaatagc tggagatgag aataaatggc
     7081 ttgagcagtt ggatgctgac ttctttgatt tgattattgt tgatgaagcc caccacaaca
     7141 aggcaagcac atgggttaag gttaaagaaa agttcccaaa tgcgaaggtg ataaactata
     7201 gcgccactcc aatgaggagt gatggccagt tgatgagcgg tgaaattata tatagtttcc
     7261 ctgtggttga agcaattagg gaagggtata ttaaaaggct tcacgccaag atgattagtc
     7321 catcaaagtt ggtatatgtt gatagtgatg gtaatgaaat aaccttaaca aaccctgagg
     7381 atattaaaag attaggtgag gaagaagcat catttaggcg cggcgttctc atgtctgatg
     7441 aaacacttag tacattggta gatctttcta ttaaggaact aaatagactt cgagaagagt
     7501 ctggggaaaa aaggttaaaa atcattgcat ctgcactgaa ttacggtcat tgcattcaga
     7561 taaaagaagc atttctggct agaaacctac gagctgatta tgttcactct aacgaagatg
     7621 gggctgttaa tgatagggtt ttctctaaac tagagaatca tgagctagac gttattgttc
     7681 aatcgaaaat gcttggtgaa gggtttgacc acaaattttt atctgtagct atggttggta
     7741 gcgttttttc taatttgagc ccatttgttc agtttgttgg ccgagtaatg cgagtagtaa
     7801 aacaaaattc ccccggcgat ttagttaaca gaggagttgt tgttttccat gttggtgcaa
     7861 acattgccaa tcggtggagt gactttagga atttcagtca ggccgatcag gacttcttcg
     7921 ctgacttgtt accagaagtt gaagatgttg attttggctc cgatggcaca agtgacaggg
     7981 atcctgatga gacagggggt agtaaccagc ttatacctgt cgaggttact agcactgatg
     8041 gtgtaacggc ttccgagtta gatccaatcg gagatttcca agagcttaaa aaagaactgg
     8101 catctttagg tatgacaact aatgaggcga tagagatttt gcgtaggtct aggttgagca
     8161 aacaagatca gagaagagct ggaagacagg ctattaacga tcttgttaat catcatgcgg
     8221 gcttgctcat taatcaactt gggttcaaac cgaaaggccg aaatttcaac aagtctcaag
     8281 ataactactc atgggttgta agagagttgc ataatcgagt taacgaacat attgggtgta
     8341 acagcggtga tagagaaaat cttagtttag agcaggttga tgaagctaaa tcagctgtgc
     8401 ctaaccttgc agaagacatc cgacaggaat ggtctaatgg ctaaattcaa gtctcttatt
     8461 gttggcgcca agtttgaggt tgcctctagg aaaaccacct gtaaacatga caaaaagcat
     8521 gtgattacaa aaggtgagtt caggctaagc gtaaagaatt cgacgcaagg tgaatcttac
     8581 tattgcctct cctgtgccaa aacgatggtt actgaaagcc aggccaaact agaaggtctc
     8641 aaaagcgagc tcgattccct ctcactttag gtgagaccat aacaagccta tatcagataa
     8701 cagccctctc tagcagaggg cttttttttt tgcagccagt atataatctg acatactgca
     8761 tcatactcta agccagtaca cacagcgtcc cctctatcga ggtgcctccg tgactggttc
     8821 agggggctcg ccgccccccg aaacccctgg catccactgc gaaaaattct cacggtggtg
     8881 cgaattttct ctcagcggag cctatggaaa agcgaacgaa agagatcaaa atccggctca
     8941 ccgaaaccga gcatcagcga ctgcttgatc gctgcgaccg gactcacctt gcggagtggc
     9001 tgcgtcagct aggtctgggt gaacacacct cacgcaagcg ccgtgtgcct gatgtggcgc
     9061 ccgaactgct tcgacaggtc agcgggattg gcaacaacct gaaccagatt gcccgccgcc
     9121 tgaaccagac tgattccctg acgccttctg aacgtgcttc gctgctcgtc gtactgacca
     9181 gccttgaccg tcagcttggc gacctgctgg agcagaaccg tgatcgttaa aattcactca
     9241 cgcggtgcag gttccggcag tggtcctgtg gattacctgc ttggaaaaga ccgtcagcgc
     9301 gaacaggcca gcgtactgcg cggcaacccc gaacatgtgc gcgagctgat agacggctgc
     9361 gactttgccc gcgcctatac ctccggcgtg ctgtcgtttc aggagccgga cattgcagat
     9421 gccgagaaat cacgcctgat ggacgaatgg gagcacacgc tgctgcctgg cctcgaccgt
     9481 gaccagtacg cctgcctgtg ggtggagcac cgcgacaagg gacggcttga gctgaatttt
     9541 gttatcccga acatcgaatt acagagcggc aagcgcctgc aaccctactt tgaccgcgcc
     9601 gaccgccccc gcgtcaacgc atggcagacc ctgaccaacg accggctggg gctgcgcgac
     9661 ccgaacgacc cgacttaccg ccgcccgctg acgcaggcca gcgatctgcc ccgcgacaaa
     9721 cagcaggcgg cagagaaaat caccgccggc ctgatgaacc tgatgcagca gggtgtgatc
     9781 cgcagccggc aggacgtagt gacgcagctg gaaagctacg gcctgaccgt ggcgcgggaa
     9841 acgaaaagca gcatcagcat tgccgatccg gacggcggcc gaaatatccg gctgaaagga
     9901 atgatttatg agcgagattt taaatttggc gaagggcttc gaggagaaat cgaagcagca
     9961 ggcgcaggat accgagcaga gcgtgaagcg cgagttcgtg aggctggaga cgtctatcag
    10021 cgcggaactg caatcaagct cgcagagcat cagcaacgct atccgcgagc agaacggcag
    10081 gctgacgggc atgctcagga agtcagtctc aacagcgatg gcgtggatat tcttctgctt
    10141 cgcactgctg atcggcatca tgggcgggat aatctggttt cagggcacga tcatcacctc
    10201 gcgcctgagc gagatggaca gctacagcca gcagctgggg gtcggttccg gctgagggcg
    10261 aaatgacacc ctaagctttc ggttccttgg gccaaagata ttcgccagtc agtagaatgt
    10321 gcgcccagcc caatggggat atgtggggaa gaaattcagg gggaacatcc aacccttcgt
    10381 tccgccgctc cgtgacggca tgaccaagat ggacggtatt ccagtaaatg atcaccgcag
    10441 tcaataaatt gagcccagcg attcggtagt gctgcccctc tgtcgtgcga tcgcgaattt
    10501 ccccctgcct cccgatacgg agcgcatttt tgagcgcatg gtgggcctct cccttgttaa
    10561 gaccgatctg agcacgccgc tgcatgtccg tatccaggat ccactcaata atgaaaaggg
    10621 tccgttcaat acgaccaact tcacgaagcg caactgcaag gttgttttgt cgtgggtaag
    10681 aagcgagctt gcgcaggagt tggctgggcc tgattttgcc agcggtcatc gtcgcggcac
    10741 aacggaatct atggtcactc ccgtttttgc aacaccgatt ttgacgataa attggcttgc
    10801 ttgaatctat ccggcgtctg aatgggattt tattcccgcg cctcgatgag ttccgcgcct
    10861 gatgaacctc cagaaaatat acggcttcaa tgagcctttc cg
//
