LOCUS       NZ_AP021939             2726 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Acinetobacter pittii strain WP2-W18-ESBL-11 plasmid
            pWP2-W18-ESBL-11_3, complete sequence.
ACCESSION   NZ_AP021939
VERSION     NZ_AP021939.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194360
            Sequence Read Archive: DRR199196
            Assembly: GCF_014168695.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter pittii
  ORGANISM  Acinetobacter pittii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Acinetobacter pittii str.
            WP2-W18-ESBL-11 isolated from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 228x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 06:48:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,040
            CDSs (total)                      :: 3,944
            Genes (coding)                    :: 3,842
            CDSs (with protein)               :: 3,842
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 43 of 102
            Pseudo Genes (incomplete)         :: 61 of 102
            Pseudo Genes (internal stop)      :: 26 of 102
            Pseudo Genes (multiple problems)  :: 23 of 102
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP021939.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Acinetobacter pittii"
                     /mol_type="genomic DNA"
                     /strain="WP2-W18-ESBL-11"
                     /isolation_source="wastewater"
                     /db_xref="taxon:48296"
                     /plasmid="pWP2-W18-ESBL-11_3"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6640 N 139.4378 E"
                     /collection_date="2018-02-13"
     gene            131..448
                     /locus_tag="H7R23_RS20080"
                     /old_locus_tag="WP2W18E11_P30010"
     CDS             131..448
                     /locus_tag="H7R23_RS20080"
                     /old_locus_tag="WP2W18E11_P30010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000070380.1"
                     /translation="MSNPSKLDTLVAEISGTAESEYIGLYKQIPFRVRLPLFARLSALH
                     TIQNSHNKTPRNALLNDLLEIAMDQVMFKLDEETLEALGHLEAQYYEELSQYDSGELSD
                     D"
                     /name="hypothetical protein"
     gene            611..901
                     /locus_tag="H7R23_RS20085"
                     /old_locus_tag="WP2W18E11_P30030"
     CDS             611..901
                     /locus_tag="H7R23_RS20085"
                     /old_locus_tag="WP2W18E11_P30030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000593064.1"
                     /translation="MIKLEGTVLNTYHLDGGTNKKGEEYEARDKVQLLGSLELPNGQIK
                     NELIDLTVEDSRIYDDFKNKLISISCGAMAVGRNVIFYVRKGAKPVLADHL"
                     /name="hypothetical protein"
     gene            986..2236
                     /locus_tag="H7R23_RS20090"
                     /old_locus_tag="WP2W18E11_P30040"
     CDS             986..2236
                     /locus_tag="H7R23_RS20090"
                     /old_locus_tag="WP2W18E11_P30040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180320.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001180320.1"
                     /translation="MQNLDKKKPLLSDSLATGDNKGFASPKGDQHRDRITRFGILKHRS
                     KQQENYLFSLAKIKENYHADVKNDESIRAMKTAQKLNGCGNFLLFKNFYTIDQIKLAKF
                     QACSEHLLCPFCAGIRASKAIQKYSERVDQVLSENPRLKPVMITFTVKNGVDLRERFTH
                     LIKSFRTLIERRRDYIKKGRGFNEFCKINGAMYSYENTYNEKTNEWHPHIHVFALLDDW
                     IDQDELSQYWQSITGDSMVVDIRRAKKQKDLGYSGAAAEVCKYALKFGDLSVEKTWEAF
                     KVLKGKRLSGAFGSLWGVKIPESLIDDLPDDSDLPYLEMIYKFVFSKKSYYDLQLTRHV
                     EPTGKDAADELRGVEGRNLLVSMDGRGVSDAGRARTGALAPQHGRKKQHWQIPPVTRVR
                     VRKRIRRWDGYLCVLHL"
                     /name="protein rep"
     gene            2333..2548
                     /locus_tag="H7R23_RS20095"
                     /old_locus_tag="WP2W18E11_P30050"
     CDS             2333..2548
                     /locus_tag="H7R23_RS20095"
                     /old_locus_tag="WP2W18E11_P30050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001262268.1"
                     /translation="MANANTTVLELLGKQVSFVYVLKSDSDEYSFTCSGVVTDVIISLN
                     SELQLSVDNGDFYIYSDLKDFSIKSE"
                     /name="hypothetical protein"
     Typing          987..2236
                     /name="rep_cluster_420"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.762"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgattacaag ctccctttag cctaagaacg ggagttaccg agctttgctc gccgtgatat
       61 tgaatagatt ttaattttga tatataaata gaatcacaaa atgattcctt ttatatataa
      121 ggtaagattt atgagcaatc catcaaaatt agatacactt gttgcagaaa ttagtggtac
      181 tgctgaatct gaatatattg gtctttataa gcagattcct tttcgtgtaa gacttcccct
      241 ttttgcacgt ttatcagcac ttcataccat tcaaaattcc cataataaaa ctccgcgtaa
      301 tgcgttgttg aatgacttac ttgaaatcgc catggatcaa gttatgttta aattagatga
      361 ggaaactttg gaagctctgg gacatttaga agctcaatat tatgaagagc tttctcaata
      421 tgattctgga gaactttcag atgattagaa aaccacgaat tataagtaac acctctatgg
      481 aaagcctgga tgaagcatgt acttacttta gacaggttct ccaggaacat ttagataaaa
      541 agagacaaat tacatttttt accattgcta tggtcgatgg tcgtttgaag ttaattgagg
      601 ggataccaga atgattaagc ttgaaggtac agttttaaat acataccact tagatggtgg
      661 gactaataaa aagggcgaag aatatgaagc tcgagataag gtccagcttc taggttcgtt
      721 ggagcttccc aacggacaaa ttaaaaatga attaatcgac cttactgttg aagattctcg
      781 tatttatgat gatttcaaaa ataagcttat tagcatcagt tgtggcgcta tggctgttgg
      841 tcgtaacgtt attttttatg ttcgaaaagg tgcgaaacct gttttagcag atcacctatg
      901 attttcccta aaaaacatcg agttagcgaa gcgtctcgat gttttttctt ttacttgata
      961 ctattgatta aagcggggac aaaatttgca gaatttagat aagaaaaaac ccctgttatc
     1021 ggacagtttg gcgaccggtg ataacaaggg ttttgcatct cccaaaggag atcaacatag
     1081 ggatagaata acacgttttg gcattttgaa acatagatcg aagcaacaag aaaactattt
     1141 attttcgtta gctaagatta aagaaaatta tcatgccgat gtaaaaaacg atgaatctat
     1201 tcgcgccatg aaaactgccc aaaaattaaa tgggtgcggt aattttcttc tattcaaaaa
     1261 tttttacacc attgaccaaa ttaaactcgc caagttccaa gcttgtagtg agcatttgtt
     1321 atgcccgttt tgtgcaggta ttagagcttc taaggcaatt caaaaatact ctgagcgtgt
     1381 tgatcaagtc ttatctgaaa atcctcgttt aaagcccgtt atgatcacgt ttacggttaa
     1441 aaatggggta gatctaaggg aacggttcac tcatcttata aaatcgttta gaacgcttat
     1501 agagcgtcgt agggactata ttaaaaaagg gcgtggcttt aatgaatttt gcaaaattaa
     1561 cggtgcaatg tattcatatg agaatactta caatgaaaaa actaatgagt ggcatcccca
     1621 tattcatgtg tttgcacttt tggatgattg gatagatcag gatgaattgt ctcaatattg
     1681 gcaatctatt actggggact ctatggtcgt tgatatccgt agagccaaaa aacaaaaaga
     1741 cttaggctat tcaggtgctg ctgctgaagt ctgtaaatat gctctcaaat ttggtgatct
     1801 ttctgtagaa aagacttggg aagctttcaa agttttaaaa ggtaagcgat taagtggtgc
     1861 ttttggatct ctttggggcg tgaaaattcc tgaatcatta attgatgatt taccagatga
     1921 ttctgattta ccttatctag aaatgattta taagttcgtc ttttctaaga agtcttatta
     1981 cgatttacaa cttactcgtc atgtcgaacc tacaggtaag gacgccgccg acgagcttcg
     2041 aggagtagaa ggacgcaacc tgttggtgag catggacggg cgaggagtga gcgacgctgg
     2101 gagggcccgc actggcgcgc ttgccccgca gcacggacga aaaaaacaac actggcaaat
     2161 cccaccagtt actcgtgttc gggttcggaa gcgaatccga agatgggacg gatatttatg
     2221 tgttttacat ttatagtttt taactagtta acaatactag taagtcggtg aaatcaggga
     2281 gtgccatccc cgatttcacc taaccacaac gtactgtcaa ggagtactta tcatggctaa
     2341 cgcgaatact actgttttag aacttttagg caaacaagtt tcttttgttt atgttcttaa
     2401 gtctgattca gatgaatatt ctttcacctg ttctggtgtt gtgactgatg taattattag
     2461 tcttaactct gaactacagc tctctgttga taatggggat ttttatattt attcggattt
     2521 aaaggatttc tctatcaagt ctgaataagg ggctcttggt atatttacga aatctcatgc
     2581 cacgcttgaa actcaagggt ttcaggcgtg ttttttttat agtttcttga gaaacgcgta
     2641 agcgtgcatt actgaaaaag cacctaaatc ttagagtggg tgtctaccca cgaactgaca
     2701 taaaacgatt tcaaaccttc cctctc
//
