LOCUS       NZ_AP021938            10416 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Acinetobacter pittii strain WP2-W18-ESBL-11 plasmid
            pWP2-W18-ESBL-11_2, complete sequence.
ACCESSION   NZ_AP021938
VERSION     NZ_AP021938.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194360
            Sequence Read Archive: DRR199196
            Assembly: GCF_014168695.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter pittii
  ORGANISM  Acinetobacter pittii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Acinetobacter pittii str.
            WP2-W18-ESBL-11 isolated from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10416)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 228x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 06:48:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,040
            CDSs (total)                      :: 3,944
            Genes (coding)                    :: 3,842
            CDSs (with protein)               :: 3,842
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 43 of 102
            Pseudo Genes (incomplete)         :: 61 of 102
            Pseudo Genes (internal stop)      :: 26 of 102
            Pseudo Genes (multiple problems)  :: 23 of 102
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP021938.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10416
                     /organism="Acinetobacter pittii"
                     /mol_type="genomic DNA"
                     /strain="WP2-W18-ESBL-11"
                     /isolation_source="wastewater"
                     /db_xref="taxon:48296"
                     /plasmid="pWP2-W18-ESBL-11_2"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6640 N 139.4378 E"
                     /collection_date="2018-02-13"
     gene            101..1030
                     /gene="repM"
                     /locus_tag="H7R23_RS20015"
                     /old_locus_tag="WP2W18E11_P20010"
     CDS             101..1030
                     /gene="repM"
                     /locus_tag="H7R23_RS20015"
                     /old_locus_tag="WP2W18E11_P20010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065049.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_182918118.1"
                     /translation="MSELIVKDNALIQASYTLDTVEQRLILLAIAEARETGHGITENSL
                     LEVHASSYINTFNVEKHTAYTVLRDASKSLFDRYVTYHDINPKTGKDRSFHCRWVDKIG
                     YEPQSGIVFLRFTQDIVPLITRLEENFTKYELEQVSRLTSSYAIRLYELLIQWRSAGKT
                     PIFDLSIFRQQLGVEAHQYKTMSNFKTYVLDFALKQVNELTDITAKYEQHKKGRSISSF
                     SFSFKQKKTNSDKVIKGTDTLALFTKMSDKQRHLFANKLSELPEMGQYSEGTESFQQFA
                     IRIAEMLQDYQKFQELFPYLQKVGFAPA"
                     /name="repM"
     gene            1109..1639
                     /locus_tag="H7R23_RS20020"
                     /old_locus_tag="WP2W18E11_P20020"
     CDS             1109..1639
                     /locus_tag="H7R23_RS20020"
                     /old_locus_tag="WP2W18E11_P20020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017397202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_017482483.1"
                     /translation="MKSLTVLELSKLYNINRQTIYNNIKKGILSKNSQNKIDLAEAIRV
                     FGEPVKKQDVKEPVKIDSPNSAEVLLLRQQIDMLKNQLDDAKDRESFYQNQIETMQRLL
                     EGPKPNMTTFTDQELVQPSIAKPDPEPDTAEAKHDGLTTPERRENKWIQVPEHIEPEPE
                     KRGFWSRFFRPYD"
                     /name="plasmid replication DNA-binding protein"
     gene            1988..2305
                     /locus_tag="H7R23_RS20025"
                     /old_locus_tag="WP2W18E11_P20030"
     CDS             1988..2305
                     /locus_tag="H7R23_RS20025"
                     /old_locus_tag="WP2W18E11_P20030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005065053.1"
                     /translation="MLGENHHFLKKFSISDAAIAYKKPRSTILKYIKSGDFELDYNKLI
                     EYSQLVRKFGELTYPIQDEREQQIKKLKDEVMHLKKQLNFFKYTSISNKNRLLISHKHS
                     I"
                     /name="hypothetical protein"
     gene            2830..3351
                     /locus_tag="H7R23_RS20030"
                     /old_locus_tag="WP2W18E11_P20040"
     CDS             2830..3351
                     /locus_tag="H7R23_RS20030"
                     /old_locus_tag="WP2W18E11_P20040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000999915.1"
                     /translation="MNDSDDRNHKVILEYLQKVLERERDRFYKLDDKVSKLFTTVSTVI
                     TGFLIIVNILLDHNKISIFCSSEITILSLLSLTTFCFLCMSWNFLFKVFNLKKTAHLQH
                     QGFWKYYKNREEIDFYERIIKDHETVIDTYIESNDQKVLLLKKSLSNIYLSAIMFVILV
                     IIYLIFKYLK"
                     /name="hypothetical protein"
     gene            3348..3494
                     /locus_tag="H7R23_RS20035"
                     /old_locus_tag="WP2W18E11_P20050"
     CDS             3348..3494
                     /locus_tag="H7R23_RS20035"
                     /old_locus_tag="WP2W18E11_P20050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000192351.1"
                     /translation="MTQKNSNTSTNPANQQPHKDIKSEVPPPPVRYGLDEKIKAEVPKS
                     NKK"
                     /name="hypothetical protein"
     gene            complement(3542..3832)
                     /locus_tag="H7R23_RS20040"
                     /old_locus_tag="WP2W18E11_P20060"
     CDS             complement(3542..3832)
                     /locus_tag="H7R23_RS20040"
                     /old_locus_tag="WP2W18E11_P20060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family toxin"
                     /protein_id="WP_000176412.1"
                     /translation="MTLTIRYKKQFKKDFKRVSSNPKLKKGLLILKDLIDEHLVKTGTV
                     PDEYLPHPLIGEWKPHMECHILPDLLLIWDVDEDNNELILARIGSHSELFG"
                     /name="type II toxin-antitoxin system YafQ family toxin"
     gene            complement(3816..4091)
                     /locus_tag="H7R23_RS20045"
                     /old_locus_tag="WP2W18E11_P20070"
     CDS             complement(3816..4091)
                     /locus_tag="H7R23_RS20045"
                     /old_locus_tag="WP2W18E11_P20070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140357.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="WP_005140357.1"
                     /translation="MNTLAKKDEYVRARVPNELKVQAERILKEVGLNTTDAIRLLLTQV
                     VNRGGFPLELRTPNRTTIEAMNSVAEPKKFSSAQELFTDLEDDTDD"
                     /name="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
     gene            complement(4279..4518)
                     /locus_tag="H7R23_RS20050"
                     /old_locus_tag="WP2W18E11_P20080"
     CDS             complement(4279..4518)
                     /locus_tag="H7R23_RS20050"
                     /old_locus_tag="WP2W18E11_P20080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008486742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_017482479.1"
                     /translation="MSEQQIYERIRLAMNEAPRNRQTAELHLQMIKYADDLKNITSKEF
                     CEGVGLPLSYGTEFSKMRNITERLKAAGLKVNMI"
                     /name="hypothetical protein"
     gene            complement(4755..5222)
                     /locus_tag="H7R23_RS20055"
                     /old_locus_tag="WP2W18E11_P20090"
     CDS             complement(4755..5222)
                     /locus_tag="H7R23_RS20055"
                     /old_locus_tag="WP2W18E11_P20090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004727917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="WP_017399702.1"
                     /translation="MIEKGAIISDCNKYRYSLWRIWDKEKPIFTFVGLNPSTADHEQDD
                     RTIRRCINFAKSWGGGGIYMANLFAYRATDPQEMMSQEDPIGPDNDAHLKQLAAESHKL
                     IACWGNDGVFKARSTFVKQLLTDKLYYLKLNKSGEPGHPLYIHSSTQIQPF"
                     /name="DUF1643 domain-containing protein"
     gene            complement(5274..6365)
                     /locus_tag="H7R23_RS20060"
                     /old_locus_tag="WP2W18E11_P20100"
     CDS             complement(5274..6365)
                     /locus_tag="H7R23_RS20060"
                     /old_locus_tag="WP2W18E11_P20100"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpaII family restriction endonuclease"
                     /protein_id="WP_017482473.1"
                     /translation="MITANKGEWSEVYALFKLLSEGKLYAGDKDLNKIPNLIYPIISIL
                     RQESQDLLTYSPEPNHGVVHIQNGTSTFTISQSDFKCITEFLLTEIKKKQPTASFSIPE
                     VETFISQYNSKKIKAKSSAKSDIRIIIYDQKIGTTPELGFSIKSKLGKASTLLNASQAT
                     NFVYKVKNLTLSPQEIDDFNQLEFTVPIIQGRIHHLESLGGSIEFSHITNDVFNNNLIL
                     LDSLLPNIISEALYKYYTSPITSVKEIFEQVASANPMHYSLQHQHPFYEYKVKKLLCEI
                     AIGMMPSTVWTGNNIDATGGYLVIKEDGDVICYHLYHRHEFEEYLYNNTRFEAASKSRH
                     NYGNLFIENGELYMTLNLQIRFK"
                     /name="HpaII family restriction endonuclease"
     gene            complement(6365..7447)
                     /locus_tag="H7R23_RS20065"
                     /old_locus_tag="WP2W18E11_P20110"
     CDS             complement(6365..7447)
                     /locus_tag="H7R23_RS20065"
                     /old_locus_tag="WP2W18E11_P20110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017482472.1"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WP_017482472.1"
                     /translation="MNSSNSHFNEVDPTLINETIPLFEKVPFNPIYPSKFKFIDLFAGI
                     GGFRLAFQNLGGRCVFSSEIDLAAQKTYTANFGDTPHGDITLESTKDAIPDNFDILCGG
                     FPCQAFSIAGKRSGFEDTRGTLFFDVADIIKRKQPKAFFLENVKGLVSHDKGKTLKTIL
                     QVLREDLGYYVPEPKIINAKDFGVPQNRERIFIVGFHKNTGVQDFEYPTPTGKKVCFAD
                     VKEENVVPTKYYLSNQYLQTLERHKAHHESKGNGFGYAIIPDNSIANAIVVGGMGRERN
                     LVIDHRITDYTPTTRIKGEVNRQGIRKMTPREWARLQGFPDNFKIIVSDAAAYKQFGNS
                     VAVPAIQATANLILEKLKMK"
                     /name="DNA cytosine methyltransferase"
     gene            complement(7764..8648)
                     /locus_tag="H7R23_RS20070"
                     /old_locus_tag="WP2W18E11_P20120"
     CDS             complement(7764..8648)
                     /locus_tag="H7R23_RS20070"
                     /old_locus_tag="WP2W18E11_P20120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_017482471.1"
                     /translation="MFYLNLKHNIKKEQSSLNAFHYLTRTAHFENQKDNEKLEFIRYGN
                     MPKWAENKPKLFWKSADQFEISRGRTSSTLTIALPKELIREQRAELVQKLIDQFAGQYQ
                     FPYTAVIHNHPSEITGEDQPHLHLMYSERTISDDIERPPEQFFKQYRPKNPTQGGAQKL
                     TADALGFGRNQVKVFREKTQELMNLFLEKYAPTKKVMVYGVEIEVKNQVSCLSNDDFNQ
                     KFGTKLQDVPQIPRWKLYSADPIIQLDVEAQKNTIKKIRNQNNAELYGKEYDLEISRRH
                     VLTQKKDPGFSLD"
                     /name="MobA/MobL family protein"
     gene            9104..9817
                     /locus_tag="H7R23_RS20075"
                     /old_locus_tag="WP2W18E11_P20130"
     CDS             9104..9817
                     /locus_tag="H7R23_RS20075"
                     /old_locus_tag="WP2W18E11_P20130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006581718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_006581718.1"
                     /translation="MAGRKTFALTQQEIIDLDKHIRALPFKRRMQDAFLAMISERESMK
                     QLNEEDLKLIQRCRYERNTYNKRIVQLQLIQKTEPQKLKKLELNILELSKKTDIDAYFA
                     MHDALNEVLKIKRNQTSEKNITAKVERVLNPEKYNQAPLSKSLRKQQDHIKYFIGSLYL
                     NLLEQSGLERSNSNQDLDRMLDLFKQAQSGAEKNNAFSLDQNRKTDILSAKSLYEMEKL
                     IKQAKEDPRNPFNKA"
                     /name="hypothetical protein"
     Typing          102..1017
                     /name="rep_cluster_1226"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.776"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(7768..8648)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.98"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccttaaaact aggtttatcg accccaaatt aagtcgattt acaattcaat taaaatagtc
       61 tattgtacaa cctagtttaa ttataaattt attattagaa atgagtgaat taatcgtaaa
      121 ggataatgct ttaattcaag ctagctatac cctagataca gttgaacaaa ggctgatcct
      181 attagccatt gctgaagctc gggaaacagg gcacgggata actgaaaata gccttttaga
      241 agtacatgca agtagttata tcaatacctt taatgtcgag aaacacactg cttataccgt
      301 gcttcgggat gcatctaaaa gcttgtttga tcgctatgtc acgtatcatg acattaatcc
      361 taagactggt aaggatcgta gctttcactg tcgttgggtc gataaaattg gatatgaacc
      421 tcaatccggg atcgttttcc tacgtttcac gcaagacatt gttccactca taactcgact
      481 ggaagaaaac tttactaaat atgaattgga gcaggtttcg agattaacta gctcttatgc
      541 tattcggtta tacgagttat taattcaatg gagatctgct ggtaaaacgc caatttttga
      601 tctatcgatc tttagacaac aacttggtgt cgaagctcat caatacaaaa caatgagtaa
      661 ttttaaaacg tatgttttag attttgccct caagcaagta aatgagttaa ccgatataac
      721 ggctaaatat gaacaacata aaaaaggacg ttcaatttcc agtttttctt tcagttttaa
      781 gcagaaaaaa accaactctg acaaagtaat taagggaacg gatacattag cccttttcac
      841 caaaatgagt gataaacaac gtcatctctt tgccaacaaa ctttctgaat tacctgagat
      901 ggggcaatat tcagaaggca ccgaaagctt ccaacaattt gctatacgca ttgcagaaat
      961 gctccaagat tatcaaaaat tccaagaatt attcccttat cttcaaaaag ttggctttgc
     1021 acctgcctaa aatttaaact tgatcatttg tctattagac agtctaatag acaacaactt
     1081 ttgacaggtt taggcataga tttacaacat gaaaagcctt acagttctcg agctttctaa
     1141 gctttacaat atcaatagac aaacgattta caacaacata aagaaaggaa ttttaagtaa
     1201 aaattcccag aataagatcg atctagccga ggctatccgt gttttcgggg aacctgtcaa
     1261 aaaacaggat gtaaaagaac ctgtaaaaat agacagtcca aattcggcag aagttttact
     1321 acttagacag cagatagaca tgctgaaaaa ccagcttgat gatgccaaag atcgggaatc
     1381 tttctatcag aaccaaattg aaacgatgca acgcctatta gaaggcccaa aacctaatat
     1441 gactacgttt accgatcaag aattagtgca acccagcata gccaaaccag atcccgaacc
     1501 tgacacagca gaagctaaac atgacggatt gactactcca gagcggaggg aaaataagtg
     1561 gattcaagtg ccagagcaca ttgaaccaga gcctgagaaa agaggctttt ggagccgttt
     1621 ttttagaccc tatgattaac cacgattgtt taattaatat gaataaagtc gtgaaacttt
     1681 gattaaataa taagtgtgga acgtgaaaaa tcataaaaaa aagcccacct tggggaggtt
     1741 gggcaaaaaa gaaactacag cgttgaattt tcaagagaat tataacgcat ttcgtataag
     1801 gtgtattatg ttaataatat aaatttttaa aagcatattg atttaataat agaaaaatta
     1861 tcagagtagg gcatgtgtaa agtaaacgtt cgattattaa ccaataatga ttaaagattt
     1921 atatcttgta tgtgttaatt ttattaacat atatatacga aattaataat gccttaagtg
     1981 aaatagtatg ctaggtgaaa atcatcattt tttaaaaaaa ttttcgatca gtgatgctgc
     2041 tatagcgtat aaaaaacctc gctcgactat acttaaatat ataaaaagcg gagatttcga
     2101 acttgactac aataaattga ttgaatacag tcagttagtt agaaaatttg gtgaacttac
     2161 ttaccctata caggacgaaa gagagcaaca aatcaaaaaa ctaaaagatg aagttatgca
     2221 tttaaaaaaa caacttaact tttttaaata tacttcaatc tcaaacaaaa atagactatt
     2281 aatttctcat aaacattcca tttaacataa tgggcgttat ccgaaatcgt cttttataag
     2341 ttaaataaaa tcatgtactt agctatttta cataacccga aactaccgca aaaatccgct
     2401 ttgtttccaa agcggatttt ttttgagcaa ggttgatact tcttgccaat aaaattggtt
     2461 aaataatgat caaaaatagc aattccatag agtttttaag ccttttttac ccaaaagaat
     2521 ccacaattta tgcggataac cattgggata aattttgatt attttttgta cgttttggct
     2581 tacagcgttt tacgaaattg tctgtatcta tcccccgatg aagtccttat gatcaatcta
     2641 tatgaacaag atgttcaatg acttaaaaca acaataataa caataataat cagtcaggca
     2701 ccatgaccat caggtagtca gccctaccta ggcgctccac accgaaaaat cccgacatga
     2761 tgtcgggatt ttttattgtt aaaatttaaa ttaaataaat tttgttattt gaaataaagg
     2821 ggattgagta tgaatgactc tgatgatcgt aatcataagg taattcttga gtatctacag
     2881 aaagtactag aaagagagcg agatcgattt tataaattag atgacaaagt atctaagtta
     2941 tttacgacag tatctactgt gattacagga tttctaatta tagttaatat ccttttagat
     3001 cataataaaa ttagtatttt ttgttcttcc gagattacta ttctaagttt attgtcactt
     3061 acaacattct gcttcttgtg catgtcttgg aacttccttt ttaaagtttt taatttaaaa
     3121 aaaacagctc accttcaaca tcaagggttt tggaaatatt ataaaaatag agaagaaata
     3181 gatttttatg aaagaatcat taaggatcat gaaactgtta tagatacata catagaatct
     3241 aatgaccaaa aagttttact tttaaagaag tctttaagca acatctattt atcagcaata
     3301 atgtttgtta tattagttat tatctatttg atttttaagt atctaaaatg acccaaaaaa
     3361 attccaatac aagtaccaac ccagctaatc aacaacctca caaagatatc aaatcagaag
     3421 ttccacctcc tcctgttaga tatggtttag atgaaaaaat taaagctgaa gttccaaaaa
     3481 gtaataaaaa ataatctcta attattgaca ataaaaagcc cgaatatatc gggcttttta
     3541 cttatccaaa aagttcagaa tgagatccaa tccgagcaag tattagctca ttattatctt
     3601 catccacatc ccaaattaat aataaatcag gtaaaatatg gcattccata tggggtttcc
     3661 actcaccaat caaaggatga ggtaaatact catctggaac agtccctgtt tttaccaaat
     3721 gctcatcaat aagatccttc aaaattaaga gtcctttctt aagctttggg tttgagctta
     3781 cccgtttaaa atctttttta aattgtttct tgtacctaat cgtcagtgtc atcttctaaa
     3841 tccgtaaaca attcctgagc agaactaaat tttttaggtt ctgcaacaga gttcatagcc
     3901 tcaattgttg tacggttagg ggttcttaac tctaaaggaa atccacctcg atttactact
     3961 tgcgttaata ataagcgaat agcatctgta gtatttaaac ccacttcttt tagaattcgc
     4021 tccgcttgaa cctttaattc atttggaacc ctagcacgta catactcatc tttttttgcc
     4081 aaagtattca tatgtttatc cagtagtcag ttcagtagtc agttcagtag tcagttcagt
     4141 agtcagttca gtagtcagtt cattgatttg gctaaagcca ttgtagcaca catgtgctac
     4201 acaacaacta acttaacata aaatctctta tgcgaaattt aaaagggctt agtcatgact
     4261 aagccctttt attagctttt aaatcatatt taccttaagg cctgctgctt ttaagcgttc
     4321 agtaatgttt ctcattttac taaattcagt accgtaacta agaggtagtc cgacaccttc
     4381 acagaactct ttagatgtta tattttttaa atcatctgca tatttaatca tttgcaaatg
     4441 tagctctgcc gtttgtctgt ttctaggagc ttcattcata gccaatcgaa tacgctcata
     4501 tatctgttgc tcactcatat ctaatttatc caattaatca aatttatata aaaaatataa
     4561 tttatataaa tttgattgtc aattttttgt tcaatcttat tggcgaaaga tgacaaatat
     4621 gattaaaaaa aagccgacat gtgtctaagt cggcattttg gtgtttttta gggtggcgaa
     4681 aaatttttaa ctatatgaaa aacaacaatt ttataaaaac aagttcgtat aacgcccatt
     4741 atgttaaatg ggatctaaaa aggctgtatc tgagtggaag aatgtatata taacggatgt
     4801 ccaggttctc cacttttatt taatttaaga taatagagtt tgtccgtaag caattgtttt
     4861 acaaaagttg atctcgcttt aaaaacacca tcatttcccc aacaagcaat aagtttgtgg
     4921 gattctgctg ctaattgctt taagtgggca tcgttatcag gaccaatagg gtcttcttgg
     4981 gacatcattt cttgtggatc agtagctcta tatgcaaata gattagccat ataaatgcca
     5041 ccacctcccc aacttttagc aaaattaatg catcgtctta tggttcggtc atcttgctca
     5101 tggtctgcag ttgatggatt taagccgaca aaggtaaata ttggtttttc tttatcccaa
     5161 atacgccaaa gtgaataacg atatttgtta cagtcggaaa tgattgcccc tttttctatc
     5221 actacattgt ccttcttgat ctatttcgaa taagagtttt tatgttaaat atcttactta
     5281 aacctaatct gtaaatttaa ggtcatatat aactctccat tttcaatgaa tagatttcca
     5341 tagttgtgtc tcgatttgct agccgcttca aatcttgtgt tgttatataa atattcttcg
     5401 aattcatgtc tatgatatag gtgataacaa ataacatcgc catcttcttt aatcactaaa
     5461 taaccaccag ttgcatcaat attattgcca gtccaaactg tgctaggcat cattcctata
     5521 gctatttcac ataaaagttt tttaactttg tattcataaa aagggtgttg atgttgtagt
     5581 gaataatgca ttggatttgc agatgcaact tgttcaaata tttcttttac ggatgtaatt
     5641 ggagaggtgt agtacttgta aagtgcttca gaaattatgt tcggaagtag gctatctaaa
     5701 agaattaaat tattattaaa aacatcattt gttatatggg aaaattcaat acttcccccc
     5761 aacgattcta aatggtgtat acgaccttga atgatgggaa cagtaaattc taattgattg
     5821 aagtcatcaa tttcttgtgg tgacaaagta agattcttaa ctttataaac gaaattggtg
     5881 gcttgactag cattaagtag cgttgaagcc ttgcctaatt tggatttaat actaaaacct
     5941 agctctggag ttgttccgat cttttgatca taaatgataa ttctaatatc actttttgcg
     6001 ctggatttag ctttgatttt ctttgaatta tattgagata taaaggtttc aacttcagga
     6061 atagaaaagg atgctgttgg ttgttttttc tttatttcag ttaaaagaaa ttctgtgatg
     6121 catttaaaat cagattggct aattgtgaag gtagaagtac cattttgaat atgtactacc
     6181 ccgtgattag gctctgggga atatgtaaga agatcctgcg attcctgccg taaaatacta
     6241 ataattggat aaattaagtt aggaatttta tttaagtctt tatcccctgc atatagtttt
     6301 ccctcactaa gcagtttgaa tagagcgtag acttcgctcc attcaccttt atttgctgta
     6361 atcattattt catcttcagt ttttctagaa tcaaattagc tgtagcttgt atcgctggaa
     6421 cagcaacaga attaccaaac tgcttatatg cagcggcatc agacacaata attttaaaat
     6481 tatcagggaa tccttgtaag cgtgcccatt ctcggggagt cattttccgg ataccttgtc
     6541 tatttacttc acctttaatt ctcgttgtag gcgtatagtc cgtaattcga tgatctataa
     6601 caagattacg ttcacgcccc atacctccga ctacaatagc attagctata gagttatctg
     6661 ggatgattgc ataaccaaag ccatttcctt tgctttcatg atgggcttta tgtcgttcta
     6721 aagtctgtaa atattgatta gaaagataat attttgtagg aacaacgttt tcctctttca
     6781 catcagcaaa gcaaactttc ttacctgttg gtgttgggta ttcaaaatct tgaacaccag
     6841 tatttttatg aaatcccaca atgaaaatac gttcacggtt ttgaggaaca ccgaaatctt
     6901 tagcattaat aattttaggt tcaggaacat agtagccaag atcttctctc agtacttgta
     6961 agatagtttt taaagttttt cctttatcgt ggctaactaa accttttaca ttttctagga
     7021 aaaaggcttt aggctgtttt ctttttataa tatctgctac atcaaagaat agagttcctc
     7081 gagtatcttc aaaaccagat cttttaccag ctatagagaa agcttggcag ggaaatccac
     7141 cacataagat gtcaaaatta tctgggatag catctttggt tgattctaat gttatgtctc
     7201 catgaggagt atcaccaaaa tttgccgtat aagttttctg cgctgctaaa tcaatttcag
     7261 atgaaaatac acatcggcca ccaagatttt ggaaagctaa tctgaatcca ccaattcctg
     7321 caaagagatc aataaactta aatttgctcg gataaatagg attaaatggc actttctcaa
     7381 aaagaggaat tgtctcatta attagagtcg gatcaacttc attaaagtga ctattactcg
     7441 aattcattac aggccttaag tacaaatatc aaaagaagaa caatagcaaa gttaggcaat
     7501 aatttctttg gtcataggac atatattgtc gtgttgttca tatatggatc ataaagcgaa
     7561 taatttaatt tttctaaaat taacataata cgccttatgc gaaaaaccta gattttacat
     7621 ttatgggcaa aaatctaggt tttccttatg gatctgaatc aaaaacacaa attttccatg
     7681 ttgtggctaa aaatcactca caatatggat aagttttttc aagatttcat gttcaaaaaa
     7741 cacaaatttt ccacttataa aaatcaatct aagctaaagc caggatcttt tttctgagta
     7801 agcacatggc gtctagatat ttctaaatca tattccttgc cataaagttc agcattgttc
     7861 tgatttctaa tttttttaat tgtgtttttt tgagcttcaa catcaagctg aataattgga
     7921 tcagcgctat aaagtttcca tcgagggatt tgtggtacat cctgtaattt ggtcccgaat
     7981 ttttgattga aatcgtcatt agataggcaa ctgacctgat ttttcacttc aatttctaca
     8041 ccataaacca ttactttttt agttggtgca tatttttcta aaaacaaatt cattaattct
     8101 tgagtttttt cacggaatac tttgacttga tttcgcccga aacctaatgc atcagctgtt
     8161 aacttttgcg caccaccttg agttggattt tttggccggt actgtttaaa aaactgctct
     8221 ggtggtcgtt caatatcatc tgaaattgtt ctttctgaat acatcaaatg taaatgtggt
     8281 tgatcctctc cagtaatttc actgggatga ttatgaatga ccgcggtata aggaaattga
     8341 tattgtccgg caaactgatc tattaacttc tgaaccaatt ctgctcgttg ttctcgtatt
     8401 aactcttttg gtaaagcaat ggttaaagta gagcttgttc ttccccggga aatttcaaat
     8461 tgatcagcac ttttccaaaa aagttttggt ttattttctg cccatttggg catatttcca
     8521 taccgtataa actctaattt ttcattgtct ttttgatttt caaaatgtgc cgtccgagtt
     8581 aaataatgga atgcattcag agaactctgc tcttttttaa tattatgctt tagatttaaa
     8641 taaaacatca gttcacctca aacaaattac gtgtaacgaa acagcaaatt ttcttgtaac
     8701 aaggggctaa ttcttgttgc agagagcagt cttttcttgt tgcaaacatc gatatttcat
     8761 caaaaaagtt tgttgcactc gtctaaaatt cgctgttttt cttgttgcac ccctttccca
     8821 tttcttgtaa cggcaggcat tgcttacttt ttcaccatta aaaaaaatac agttaaaacc
     8881 cgaagaaaaa ctcataattt aataattaaa tcagtgggtt atgtagcctc cgcaggagcg
     8941 agtagaagtg tcttttttta acaatgattg ctaaagcaat cattgttaaa aaaagacacg
     9001 gaaacgagat aaacaatatt ttatgtataa cataaaatat tgtttcaaca ccatcagaat
     9061 tccgctaaca tcaatttaga tacccaaaat caggaaatca gctttggccg gtagaaaaac
     9121 atttgcctta acacaacaag aaatcatcga tttggataaa catattcgag cgctgccgtt
     9181 taaaagacga atgcaagatg cttttttagc aatgatttct gaacgagaat caatgaaaca
     9241 actgaatgaa gaagatttaa aacttattca acgatgtcgt tatgagcgca atacttataa
     9301 taaacgtata gttcaattac aactgatcca aaaaaccgag ccacagaaat taaaaaaatt
     9361 agaattgaat attctagaat taagtaaaaa aacagatatt gatgcttact ttgcaatgca
     9421 tgatgcatta aatgaagttt taaaaattaa aagaaatcag acttctgaaa agaatattac
     9481 agcgaaggtc gaacgagttt taaaccctga gaaatataac caggcccccc ttagtaaatc
     9541 tttaagaaaa cagcaagacc acattaaata ttttatcgga tcactatatt taaacttatt
     9601 ggaacaaagc ggtttagagc gatcaaattc aaaccaagat ttagatcgaa tgctcgattt
     9661 atttaagcaa gcacaatcag gagcagaaaa gaacaatgca tttagcctgg atcaaaatag
     9721 aaaaacagat attttatcgg ctaaatcgct atatgaaatg gagaaattga ttaagcaagc
     9781 caaagaagat ccaaggaatc ctttcaataa agcttagatt gactcaaata atagggttta
     9841 aatacactgt tgaagccgta tatgcctttt tctagcctaa acctatattc cccatcaata
     9901 aaaaaattag agtgctctaa acgcaaatta gagtatttta gagatcattt gattttaccg
     9961 ataagaacaa agcgagtgtc tacgagcgat ccttggaaaa ttttgcctag actccctgaa
    10021 aaaccgcttt ttaggctgtg aaacataaat aagcatggaa aagttgtaga aatttgagtc
    10081 tagatcgagc gtagcgagta aaaaagtttt atgagcgtag cgaattccga gttgcttttg
    10141 ctttttttaa agtcacgtaa gtgctcacca aaaaattgcc ccgacgaact gagcgaaagc
    10201 gaagttcaat agagtttgag cgaagcgaaa accaagggca atttttcttt tgctaggctt
    10261 ttaattattt ttaatctttt aaatgctttt aagtaagctg taacccttta ttcataatag
    10321 ttttagagtt aataaaacta ggtttatcga cccataaaac taggtttatc gacccataaa
    10381 actaggttta tcgacccata aaactaggtt tatcga
//
