LOCUS       NZ_AP021912             4572 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella pneumoniae strain WP2-S17-ESBL-02 plasmid
            pWP2-S17-ESBL-02_3, complete sequence.
ACCESSION   NZ_AP021912
VERSION     NZ_AP021912.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00194339
            Sequence Read Archive: DRR199175
            Assembly: GCF_014168575.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Klebsiella pneumoniae str.
            WP2-S17-ESBL-02 isolated from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4572)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage                   :: 276x
            Sequencing Technology             :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 08:49:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,350
            CDSs (total)                      :: 5,232
            Genes (coding)                    :: 5,115
            CDSs (with protein)               :: 5,115
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 52 of 117
            Pseudo Genes (incomplete)         :: 60 of 117
            Pseudo Genes (internal stop)      :: 24 of 117
            Pseudo Genes (multiple problems)  :: 15 of 117
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP021912.1.
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WP2-S17-ESBL-02"
                     /isolation_source="wastewater"
                     /db_xref="taxon:573"
                     /plasmid="pWP2-S17-ESBL-02_3"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6640 N 139.4378 E"
                     /collection_date="2017-07-10"
     gene            62..400
                     /locus_tag="H7R06_RS26555"
     CDS             62..400
                     /locus_tag="H7R06_RS26555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_155559896.1"
                     /translation="MAKVTRIQGGQYAVDDGRFIVKNSSGWLVVDANGSHDFGPVPTLT
                     AAKQYVESGTTPLGQHNFSTNYGRRQSKKEFNAYLASEAKNGNPLPAILWFIVLCIICG
                     VVLAVRGY"
                     /name="hypothetical protein"
     gene            complement(561..824)
                     /locus_tag="H7R06_RS26560"
     CDS             complement(561..824)
                     /locus_tag="H7R06_RS26560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein L"
                     /protein_id="WP_089597835.1"
                     /translation="MAVYTSETKQFLDSVSHDKTWWTSRYNPADDVPVSGIYRCTVCGK
                     EITSNKDDPFPPQNHHQHSQSQAIKWQLIVRTDTQGNGCGSK"
                     /name="protein L"
     gene            complement(988..1404)
                     /locus_tag="H7R06_RS26565"
     CDS             complement(988..1404)
                     /locus_tag="H7R06_RS26565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283459.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_057103209.1"
                     /translation="MSDQKNNLDLQKQTLESKWGKEALSMGWSAIPTSLFFLQGALNMT
                     PTTFNVLLHLIIHWWKPYDWPHPSQESIAVRMGVSSRTVQRSLQELENYGLLTKIRTSK
                     DNPKYKGRNIYDMSLLVKSLNILTPDLNAKLNEK"
                     /name="helix-turn-helix domain-containing protein"
     gene            1752..2426
                     /locus_tag="H7R06_RS26570"
                     /old_locus_tag="WP2S17E02_P30010"
     CDS             1752..2426
                     /locus_tag="H7R06_RS26570"
                     /old_locus_tag="WP2S17E02_P30010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="WP_096908159.1"
                     /translation="MRTIVYVDGFNFYHGLVKRSGYKWLDLYKLFEEQLLPIRARESQL
                     IQVKFFTAEIKAKFASQGELAHQAQQAYHRALVQIYGDKLQIIKGYYSERQECAMTFNE
                     GQPPSKTDRTTVWKLEEKQTDVRMALEMYRDAIRGEAEQLVLCTNDSDMIPPIEMIKAD
                     RPELKIGIVVPREQDTSRVISSLASLGDWSLPYVSYEALRLALLPDKVPTRRKPILKPN
                     HW"
                     /name="NYN domain-containing protein"
     gene            3298..3489
                     /locus_tag="H7R06_RS26575"
                     /old_locus_tag="WP2S17E02_P30020"
     CDS             3298..3489
                     /locus_tag="H7R06_RS26575"
                     /old_locus_tag="WP2S17E02_P30020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_127900087.1"
                     /translation="MKPSNNLSAIGYHPMPSEYDEQLGSTDTQLPCEYDLFDLMAQVTE
                     ENLHAPVDFGPPVGREAL"
                     /name="hypothetical protein"
     gene            3872..4240
                     /locus_tag="H7R06_RS26580"
     CDS             3872..4240
                     /locus_tag="H7R06_RS26580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001273382.1"
                     /translation="MANFNPGPRTAIAAVRDSKNDIETWKKNGIRREEIVTVLNERYGL
                     NISLVAFDKALYRLRKSTDEPSLADVDSVSAAETNFSEASGPENGFFLSENDFMRAEEE
                     SQRMVESFRDSLGRKKGF"
                     /name="hypothetical protein"
     Typing          3133..3347
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="88.532"
                     /coverage="100.0"
                     /evalue="7.33e-68"
     Typing          complement(2669..3157)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.129"
                     /coverage="94.0"
                     /evalue="0.0"
ORIGIN
        1 agatgttatg gtaaatacaa tgagctgctg aaagcggcct gatagacatt aggagaatgt
       61 attggcgaag gtcactagaa tccagggtgg acaatatgca gtggatgatg ggcgttttat
      121 tgtaaaaaat agctcaggct ggcttgtggt tgatgccaat ggttctcatg attttggacc
      181 tgttcctacg ctaaccgctg cgaagcaata tgttgagtct ggaaccactc cgttaggaca
      241 gcataatttt tccaccaatt acggtcgcag acagagcaaa aaagagttta atgcatatct
      301 tgcctctgaa gctaaaaatg gaaacccgct gccagccatc ctttggttca ttgttctttg
      361 cataatctgt ggcgttgtat tggcagttcg aggttattag gtaaggttga tatgcatatc
      421 ctttggtctc tatggactat cgacgtgaag cctacgactg aagcccgcaa agcgggcttt
      481 ttcatgcccg gcggacaacg gatgttatgg taaatccagc tgattggcag gtacaaaaaa
      541 agcccctaag ggcttttgag ttatttagaa ccacaaccat ttccttgcgt atctgtcctg
      601 acaattaatt gccatttgat tgcctgactt tggctgtgtt gatgatgatt ttgcggcgga
      661 aacgggtcat ctttattgct ggttatctct tttccgcaaa cagtacaacg atagatacct
      721 gatacaggta catcgtcagc agggttatat cgtgatgtcc accaagtttt atcgtgtgaa
      781 acactgtcta aaaattgttt agtctcagag gtgtatacag ccatttacct tgcctttcca
      841 aaatgtatag ggtgggggta tcctctttat aagtatggtt tttttagagt attttcaatc
      901 atcgggtgat gattgaaaat actctaaaat agagatgcga tggtaaatct gaattgtaat
      961 ggtttgattt ttaaaattat tccatggtta cttttcgttc agcttggcat tgaggtctgg
     1021 cgttagaatg tttagtgact taactaatag agacatatcg taaatgtttc gccctttgta
     1081 cttagggtta tccttgcttg tacgaatttt tgtaagcaat ccgtaatttt caagttcttg
     1141 gagtgagcgt tgaactgttc ttgatgaaac tcccattcta accgcgatgc tctcttgaga
     1201 tggatgtggc caatcatagg gtttccacca gtgaataatt agatgaagta gcacattgaa
     1261 tgttgtgggg gtcatattga gtgcgccctg caggaagaaa agggatgtag gaatggctga
     1321 ccaccccatg cttaatgctt ccttccccca tttagattca agtgtctgtt tttgaaggtc
     1381 taaattattt ttttgatctg acatatacca acctgctaag ctgaagtctt ctaagaagac
     1441 ttcagcttag caggttggta aattaattca tataattagt acgacaccag cgcgcttata
     1501 atatcattat atttaactat acttaatcta cactcacctc ttggtagcgc gattaagtta
     1561 cgcagctccc ttgaggctct aaacctccct ttaggtgggc agcgattaag ttacgcgtct
     1621 tccttcagga gcttgaaacc gccctcacgg ttagttttaa ccctgaggaa ctacaactta
     1681 aactgtttgc taacgttagt ttttgttgtt aaaaccgccc ttagaggcgg tttttttatg
     1741 cgcgaaataa aatgagaaca atagtttacg tagatgggtt caatttttat cacggattgg
     1801 tgaagcgttc gggatataag tggttagacc tttataagct ctttgaagaa caactgcttc
     1861 cgataagagc aagagaatca cagttgattc aggtgaaatt ctttactgct gagatcaaag
     1921 caaagtttgc ttctcagggg gaattggcgc accaggctca gcaagcctac catcgagcat
     1981 tagtacagat ctatggtgat aagctacaga taatcaaagg ttattactca gagcgtcagg
     2041 aatgtgcaat gacctttaat gaagggcagc cgccatcaaa aacggataga acaacggtct
     2101 ggaagttaga agaaaagcag accgacgtta gaatggcttt agaaatgtat cgcgatgcga
     2161 ttcgtggtga ggcagaacag ttagttctct gcactaacga ctcagatatg ataccgccaa
     2221 tagaaatgat aaaagccgat cggcctgagt taaaaatagg aatcgtagtg ccaagggaac
     2281 aagatactag tcgagtgatt agtagtctgg ctagtttagg ggattggagc ttgccatatg
     2341 tatcctatga agccttaagg ttagctttat tgcctgataa agtccccact aggcggaagc
     2401 caatactgaa gccaaatcac tggtaaacag ccggatcttc ttgtgatcct tttactctcg
     2461 tcgtaatctc atgtcctgca aacaaaaaaa ccaccgctac caacggtggt tcgtttgccg
     2521 gaatcaagag ctaccaactc ttttttccga aggtaactgg ctttcacgga gcgcagatac
     2581 caaaactgtc cttccagtgt agccgcagtc aggccaccac ttcaagaact ctgtagaagc
     2641 actctactac tctgctccgt gaatcctgtt accagtggct gctgccagtg gcgctttgtc
     2701 gtgtctttcc gggttggact caagatgata gttaccggac aaggcgcagc ggtcggactg
     2761 aacggggggt tcgtgcatac agtccagctt ggagcgaact gcctacccgg aactgagtgt
     2821 caggcgtgga atgagataaa cgcggccata acagcggaat gacaccggta aaccgaaagg
     2881 caggaacagg agagcgcacg agggagctac cagggggaaa cgcctgggat ctttatagtc
     2941 ctgtcgggtt tcgccgccac tgatttgagc gtcagatttt gtgatgcttg tcaggggggc
     3001 ggagcctatg gaaaaacggc agcgccgtat cctttttccg gcctttcctg acttgcgtgc
     3061 catcttttta tcttgtcatt cgtgagcagg taacgcccgc cgcagtctca cggcaggagc
     3121 gtagcgaccg agtgagcgag gaggcgtcat gtcaccgtta cctgcatttt gcacttacgt
     3181 cctatagccg cattttccct tgcaacatga ataactttcc tgaattacca ctccgaccta
     3241 acatattcag cctgtataca ctccgctagc gctacgtgac tggttcaggg ctgcgccttg
     3301 aaaccctcta acaacctctc agctattgga tatcacccca tgccatctga atatgatgaa
     3361 caactgggtt cgactgatac gcagcttccc tgcgaatatg acctttttga tttaatggcg
     3421 caggttactg aagagaacct gcacgccccg gtggattttg ggccacctgt cggccgtgaa
     3481 gcgttatagc tttttcgctt acccccgtac gtgtaatacg ccggtattac atcttatttc
     3541 tttattttca tgtgattaca ggtggttgta atacgttgcg tattacataa ataaacggga
     3601 aattcagttt ttacctttta attcatgcaa ttgcgatgaa aatcgcggtt gcgtatggtg
     3661 tatggccatt taagggataa tgtaacctga cttttaaaga atgaatctat ctttggcagc
     3721 gcatgagatc gataaaaagc gttccacatt aggcggttca tggatacatg aaccggaaag
     3781 gacaaaaata agacaattgt aagacatttt ttacgtttta ctcttatttt tggactatta
     3841 tgagacaagt gtaagacatt atgggcggta tatggcgaat tttaaccctg gtccaagaac
     3901 agccattgca gctgtcaggg attccaagaa cgatatagaa acctggaaga aaaatggtat
     3961 ccgtagggaa gagattgtga ctgtgctgaa tgaacgttac ggcctgaata tctcactggt
     4021 tgcgtttgat aaggctcttt acagattgag aaaaagtaca gatgaaccgt ctcttgctga
     4081 tgtggattct gtgtctgctg cagaaacgaa tttctctgaa gccagtgggc ctgaaaatgg
     4141 tttttttctg tccgagaatg acttcatgcg agcagaagaa gaatctcaaa gaatggttga
     4201 gtcattcaga gatagccttg gcaggaagaa aggattctga ttttgaattc taagtagtga
     4261 aatagtggat cctctactcg aaattgtcgg acacctgcaa ttacctaccg tgccttacgg
     4321 cttggtcaat gccttacggc cacgcaataa attgcgtccc cgttcaccgt tttcggataa
     4381 gaagaacatc ggaaacagga cttaccccgg ctgaatggtg tgaaattctg cgctatgcac
     4441 ttgcgcgcat actcatgcat gccgtaaaaa cagagcctgc gcgtttctgg cgggttttcg
     4501 ggtggtttgt tgcctgtttt accggtttcc cgtcagaaac gctctcagag cctctcaggc
     4561 ggtgcacgca ac
//
