LOCUS       NZ_AP019821             2495 bp    DNA     circular CON 04-APR-2024
DEFINITION  Enterobacter hormaechei subsp. hoffmannii strain OIPH-N069 plasmid
            pN069_4, complete sequence.
ACCESSION   NZ_AP019821
VERSION     NZ_AP019821.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00167169
            Assembly: GCF_010319625.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei subsp. hoffmannii
  ORGANISM  Enterobacter hormaechei subsp. hoffmannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Umeda,K., Nakamura,H., Fukuda,A., Yamaguchi,T., Matsumoto,Y.,
            Motooka,D., Nakamura,S. and Kawahara,R.
  TITLE     Molecular characterization of blaKHM-1 encoding plasmid in an
            Enterobacter hormaechei subsp. hoffmannii isolate from blood culture
  JOURNAL   PLoS One 15 (1), e0227605 (2020)
   PUBMED   31929575
  REMARK    DOI:10.1371/journal.pone.0227605 Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Umeda,K., Nakamura,H., Fukuda,A., Yamaguchi,T., Matsumoto,Y.,
            Motooka,D., Nakamura,S. and Kawahara,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JUL-2019) Contact:Kaoru Umeda Osaka Institute of
            Public Health, Microbiology; Tojo-cho 8-34, Tennoji-ku, Osaka, Osaka
            543-0026, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Current Finishing Status          :: Finished
            Assembly Method                   :: Unicycler v. 0.4.4
            Coverage                          :: 594.8x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore
            Technologies MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_010319625.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 04:42:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,828
            CDSs (total)                      :: 4,711
            Genes (coding)                    :: 4,617
            CDSs (with protein)               :: 4,617
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 35 of 94
            Pseudo Genes (incomplete)         :: 66 of 94
            Pseudo Genes (internal stop)      :: 11 of 94
            Pseudo Genes (multiple problems)  :: 17 of 94
            Pseudo Genes (short protein)      :: 1 of 94
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP019821.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterobacter hormaechei subsp. hoffmannii"
                     /mol_type="genomic DNA"
                     /strain="OIPH-N069"
                     /isolation_source="human blood"
                     /host="Homo sapiens"
                     /sub_species="hoffmannii"
                     /db_xref="taxon:1812934"
                     /plasmid="pN069_4"
                     /geo_loc_name="Japan:Osaka"
                     /collection_date="2016"
     gene            445..663
                     /locus_tag="G7Y81_RS24035"
                     /old_locus_tag="OIPHN069_47270"
     CDS             445..663
                     /locus_tag="G7Y81_RS24035"
                     /old_locus_tag="OIPHN069_47270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198129632.1"
                     /translation="MFGFSSGALMTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQ
                     ILSETEPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     gene            complement(954..1139)
                     /locus_tag="G7Y81_RS24040"
                     /old_locus_tag="OIPHN069_47280"
     CDS             complement(954..1139)
                     /locus_tag="G7Y81_RS24040"
                     /old_locus_tag="OIPHN069_47280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1505..1732)
                     /locus_tag="G7Y81_RS24045"
                     /old_locus_tag="OIPHN069_47290"
     CDS             complement(1505..1732)
                     /locus_tag="G7Y81_RS24045"
                     /old_locus_tag="OIPHN069_47290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          820..907
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(2..193)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.144"
                     /coverage="98.0"
                     /evalue="1.96e-57"
     Typing          complement(1968..2474)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.774"
                     /coverage="99.0"
                     /evalue="6.33e-132"
ORIGIN
        1 tgatgcttgt caggggggcg gagcctatgg aaaaacggct ttggcgcggc cttatgcttt
       61 cttcgttaag tatcttcctg gcatccccca ggaaatttct gatccatccg taagcccgtc
      121 ccgctcgccg cagccgaacg accgagcgga gcgagtcagt gagcgaggaa gcggaatata
      181 ttctgtatca cattttctcc tgacgcgttt tctttcactt tctgcgcctg tcttatgtgg
      241 cattaatgct atgtgttact gccatgctac atcttaagcc agtatacact ccgctagtgc
      301 tccgtgactg gtccggcgct gcgcccggaa cccgcctgta ccggttcagc agccgttccg
      361 gcctgactgc aatttttttt ttttcatccc tgcccgctac cctgtaaacc tttcttctgc
      421 gttgccgtta acagtaggta agccatgttt ggtttttctt ccggagccct gatgactctt
      481 agtgatgatg agcgacacct gcttgtgtcc gttgtttcgg tctggctacg gcgtgccgga
      541 ggggatgccg gagccatgat gctggatgca taccggcaga ttttgtctga aaccgaaccg
      601 gccgtaagga ctgtcatgct cgaatttctt gaatccgttc gcattcatta catttcgtcc
      661 tgagcatttt ctgtatttct gtccatactt aaacgaggcg gccgtcacgg acggtggtcg
      721 tcgttccatg atgctgttat ggacgagtga gagtaatcta atctaacttg attgagttcc
      781 gggcgtgccg cccggttttt tttgctgttt cccggtgtcc gttccgggac aggatgtgca
      841 tttgtaatac cgcgcacgcg ccggcatcac aaataccctg gtcagggctg cgccccgaca
      901 ccccgccatc gcctcacggc cgcgcactga attgcgcacc cgttcaccga ttttcacgca
      961 ttttcctcgc ctatctgtgc gttcagcatg gcgtaaagcg cttcggcctg atcgtgcagg
     1021 tgctcacagc agccggcagc ctcatccaga tccatctgct cgagccgctc aaggaggaga
     1081 agtgtctgtg cgcggataaa tttggccata ttgagggcgg cggattcctg tctggtcact
     1141 ggtgattttc cccgtctggt gatgattttt gcgtgaaaag agcatggcgt cattctggtg
     1201 catagtcatg catgccgtta aaatttaaca ggcgcgtttc tgacgggttc cggggtggtt
     1261 tgttgtggtt tttgtcatgg tttcgtcagg aacgcgctga acgcgtctca ggcggtgcgg
     1321 gcaacggatg ttatggtaaa ttcttggggg cttgattata aaataccgca cttcctctta
     1381 tgtcttgtgc tttttagaca accctgaaag cttttagctt tggtgatgca atttgctggg
     1441 attttggcgg agaaccacag gtaaagaaaa aggccacatt agcggccttt ttcggagaag
     1501 atgctcagtg cggaatttcg gagacttctt taacgtcggt tttattgatc tgctgtttga
     1561 caccattcgc gtctgtatag ccaagcaggc ctgagtctga ttctttaggt ttgccgtcgc
     1621 tgacaatagt gcgaccgtca ttggtgtgta tggcatagct tgtgcgcgtg cagccggtga
     1681 gggctgaaag cgcaactgcc gctgcgaaca ctgaaacaaa aatctttttc aaagccagct
     1741 ccttttatcc atatcagttt tggtcactaa ccatctaact ataggtcatt ttttatgtga
     1801 aaagaggctt gatggtgggc tgtatatcga gttaggcgcg aggtgctatg gtcaaagtgt
     1861 ggtgtcaggc ggggaggagg cttatatgag atgtaatccg gccgatacat ataccaagat
     1921 gatttaagta ttcctctttg tgaggttgga tgtctaattt ataaaggatc ttcttgagat
     1981 cctttttttc cgcgcgtaat ctcttgccct gtaaacgaaa aaaccaccct ggcaggtggt
     2041 ttttcgaagg ttaggtaatc ctggcagatc ccctaaccgt ggtaacagtc ttgtgcgaga
     2101 catgtcacca aatttgtcct ttcagtgtag cctcactaag gccgccactt caagaactct
     2161 tgagacatct ctcgcacatc ctgtttgcca atggccgttg ccaatggcga ttagtcgtgt
     2221 ctttccgggt tggactcaag ttgatagtta ccggataagg cgcagcggtc ggactgaacg
     2281 gggggttcgt gcatacagtc catcctggag cgaactgcct tcccggaact gagtgtcagg
     2341 cgtggaatga gaaaccgcgg ccataacagc ggagtgacac cggtaaaccg aaaggcagga
     2401 atgcggggga gcacgaggga gccaccaggg ggaaacgcct ggtatcttta agccgcatcg
     2461 ggtttcgcca ccactgattt gagcgtcaga ttctg
//
