LOCUS       NZ_AP019768             1546 bp    DNA     circular CON 20-JUN-2024
DEFINITION  Escherichia coli O111:H- strain 110512 plasmid pO111-110512_7,
            complete sequence.
ACCESSION   NZ_AP019768
VERSION     NZ_AP019768.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00172295
            Sequence Read Archive: DRR179471, DRR179472
            Assembly: GCF_007990655.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O111:H-
  ORGANISM  Escherichia coli O111:H-
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Sekizuka,T., Lee,K., Kimata,K., Isobe,J., Kuroda,M., Iyoda,S.,
            Ohnishi,M., Sata,T. and Watahiki,M.
  TITLE     Complete Genome Sequence of an Enterohemorrhagic Escherichia coli
            O111:H8 Strain Recovered from a Large Outbreak in Japan Associated
            with Consumption of Raw Beef
  JOURNAL   Microbiol Resour Announc 8 (40), e00882-19 (2019)
   PUBMED   31582455
  REMARK    DOI:10.1128/MRA.00882-19 Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Sekizuka,T., Iyoda,S., Isobe,J., Mitobe,J., Lee,K., Sata,T.,
            Kuroda,M., Ohnishi,M. and Watahiki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUN-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: HGAP Assembly v. 3.0; Pilon v. 1.18; A5 MiSeq v.
            20140513
            Genome Coverage                   :: 200x
            Sequencing Technology             :: MiSeq; PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_007990655.1-RS_2024_06_20
            Annotation Date                   :: 06/20/2024 03:15:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,667
            CDSs (total)                      :: 5,529
            Genes (coding)                    :: 5,172
            CDSs (with protein)               :: 5,172
            Genes (RNA)                       :: 138
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 104
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 135 of 357
            Pseudo Genes (incomplete)         :: 213 of 357
            Pseudo Genes (internal stop)      :: 76 of 357
            Pseudo Genes (multiple problems)  :: 57 of 357
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP019768.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Escherichia coli O111:H-"
                     /mol_type="genomic DNA"
                     /strain="110512"
                     /serotype="O111:H-"
                     /isolation_source="bloody diarrhea"
                     /host="Homo sapiens"
                     /db_xref="taxon:168927"
                     /plasmid="pO111-110512_7"
                     /geo_loc_name="Japan"
                     /collection_date="2011-05"
                     /note="Enterohemorrhagic Escherichia coli; EHEC; Inc type:
                     Col(MG828)"
     gene            complement(687..1163)
                     /locus_tag="EC110512_RS28675"
                     /old_locus_tag="EC110512_P70010"
     CDS             complement(687..1163)
                     /locus_tag="EC110512_RS28675"
                     /old_locus_tag="EC110512_P70010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000469298.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVRE
                     LLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKNAL
                     VRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(2..83)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          1243..1351
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.95e-50"
     Typing          198..455
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.385"
                     /coverage="99.0"
                     /evalue="8.44e-113"
ORIGIN
        1 aaaacgcctc agaaatcggg acaggatgtg ggaatgtcgg accgcgcacg cgcactcaga
       61 cattccccac ctggtcaggg cttcaccccg acaccccgca tccgtgcgta agtgctcaat
      121 gtaacttccg gttctcttcc gcttcctcgt cgattcgctc gctacactcg gtcatcgact
      181 tgcggcgagc gggatcacct ctcgcatgtt gtcggtgatc ccgttgttgt tctttcgggg
      241 atgagtcgtc agatagccct tcaaacacgc tcagaagctc tcagacgcac gaaacgggtt
      301 taaatgcccg ccagggcatg gaaggcgcga aaaacgctta cagaggctct caggaggtca
      361 ggcgcgccga tacccctaac caggggcttt caggtcgcta ggaaccgcgt cagctccctg
      421 caaagcctat ttgcacggca caggcagcta ttcggctggt tggtggtcgt agaccgtcgg
      481 tgttacagcg atgggggtct ggggttctcc ccagacggtt tccggttgcg ctcaaggcgc
      541 tgccggaacc gtggcggcgc ggtcgttctg gggatcgggg agcaaaaaca cgcaagggat
      601 tttgaggcgg caaaagagca atgcaggata gacaatcatg gtcaaaacgt taacacgtag
      661 cccctgattg tccgcctgcc gctggctcaa tttgaaaaca tggccatgat tcggcgctga
      721 atatcaccgt ctttgttgac cagtgtttta gtgacaaacg ctgcatggtt tttcgtagtg
      781 ttgtatccct tccagacttc atgtggatcg agggcatacg cacaaacctt accagcaacg
      841 gggattctaa ctaatgcatt ttttgattct aagtaattga gccaccgaat aattgtgctt
      901 ctgctaactc caagtttttc agctaggaat tgctgatccg caacgacagc gccacacgta
      961 ggatcgatat gctccgctaa aaatgagtac agcttcagtg ctgatatgcc ttggctatcc
     1021 ttcagcagct ctcgaacgcg ttcccaacct tttggggata cctgtgtaaa ccttgaattc
     1081 ttctttgctt cttcgagatc ctcggtgttt ttctttgcct catctgaaag ttgtttcagt
     1141 cgtttttctg aatcaataaa catgattttt tccctctgaa aatcccatgc actaacgttg
     1201 gttcaaatct aagccatcat gacacatctg tgcaacacct tgttgcgtgt tgtcgcatcc
     1261 aggatgcgac aacgttgtgt caccctggat gcgacaggta aacaaaaaac acactaaaaa
     1321 aacaacaact tacaaatcgg cgtaaaagat ctattctagg caagggtgtt gcgcgcaacg
     1381 caacgccctt ttggggtgtc ggggcgaagc cctgacccag tcacgtagcg atagcggagt
     1441 gtatactggc ttactcctct gcaagcctcg tcagccgtga ttttgcccga tttcgcgctc
     1501 tccacgttca acagctcgat catgtttttg ccgtccgttc accgtt
//
