LOCUS       NZ_AP019766             6674 bp    DNA     circular CON 20-JUN-2024
DEFINITION  Escherichia coli O111:H- strain 110512 plasmid pO111-110512_5,
            complete sequence.
ACCESSION   NZ_AP019766
VERSION     NZ_AP019766.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00172295
            Sequence Read Archive: DRR179471, DRR179472
            Assembly: GCF_007990655.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O111:H-
  ORGANISM  Escherichia coli O111:H-
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Sekizuka,T., Lee,K., Kimata,K., Isobe,J., Kuroda,M., Iyoda,S.,
            Ohnishi,M., Sata,T. and Watahiki,M.
  TITLE     Complete Genome Sequence of an Enterohemorrhagic Escherichia coli
            O111:H8 Strain Recovered from a Large Outbreak in Japan Associated
            with Consumption of Raw Beef
  JOURNAL   Microbiol Resour Announc 8 (40), e00882-19 (2019)
   PUBMED   31582455
  REMARK    DOI:10.1128/MRA.00882-19 Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6674)
  AUTHORS   Sekizuka,T., Iyoda,S., Isobe,J., Mitobe,J., Lee,K., Sata,T.,
            Kuroda,M., Ohnishi,M. and Watahiki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUN-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: HGAP Assembly v. 3.0; Pilon v. 1.18; A5 MiSeq v.
            20140513
            Genome Coverage                   :: 200x
            Sequencing Technology             :: MiSeq; PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_007990655.1-RS_2024_06_20
            Annotation Date                   :: 06/20/2024 03:15:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,667
            CDSs (total)                      :: 5,529
            Genes (coding)                    :: 5,172
            CDSs (with protein)               :: 5,172
            Genes (RNA)                       :: 138
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 104
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 135 of 357
            Pseudo Genes (incomplete)         :: 213 of 357
            Pseudo Genes (internal stop)      :: 76 of 357
            Pseudo Genes (multiple problems)  :: 57 of 357
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP019766.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6674
                     /organism="Escherichia coli O111:H-"
                     /mol_type="genomic DNA"
                     /strain="110512"
                     /serotype="O111:H-"
                     /isolation_source="bloody diarrhea"
                     /host="Homo sapiens"
                     /db_xref="taxon:168927"
                     /plasmid="pO111-110512_5"
                     /geo_loc_name="Japan"
                     /collection_date="2011-05"
                     /note="Enterohemorrhagic Escherichia coli; EHEC; Inc type:
                     untype"
     gene            complement(4..195)
                     /locus_tag="EC110512_RS28545"
     CDS             complement(4..195)
                     /locus_tag="EC110512_RS28545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165980.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            263..586
                     /locus_tag="EC110512_RS28550"
                     /old_locus_tag="EC110512_P50020"
     CDS             263..586
                     /locus_tag="EC110512_RS28550"
                     /old_locus_tag="EC110512_P50020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            795..>1229
                     /locus_tag="EC110512_RS29520"
                     /pseudo=""
     CDS             795..>1229
                     /locus_tag="EC110512_RS29520"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1282..1824
                     /locus_tag="EC110512_RS28560"
     CDS             1282..1824
                     /locus_tag="EC110512_RS28560"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_100029810.1"
                     /translation="MGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNGQKIRDA
                     ISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTALCVTVG
                     IFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVLPSGVKG
                     ENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            1831..2064
                     /locus_tag="EC110512_RS28565"
     CDS             1831..2064
                     /locus_tag="EC110512_RS28565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137429.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2553..2798)
                     /locus_tag="EC110512_RS28575"
                     /old_locus_tag="EC110512_P50040"
     CDS             complement(2553..2798)
                     /locus_tag="EC110512_RS28575"
                     /old_locus_tag="EC110512_P50040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000820629.1"
                     /translation="MKRHRLTEAEERRLAEAIAAEARAMGLTKKDKMPQETKRRLRARI
                     LKAILDERTASKNREATLTAGQDPDDTFTWQPLRRR"
                     /name="hypothetical protein"
     gene            3062..5155
                     /gene="cda"
                     /locus_tag="EC110512_RS28580"
                     /old_locus_tag="EC110512_P50050"
     CDS             3062..5155
                     /gene="cda"
                     /locus_tag="EC110512_RS28580"
                     /old_locus_tag="EC110512_P50050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
                     /protein_id="WP_047085707.1"
                     /translation="MSDYEGSGPTEGIDYGHSMVVWPSTGLISGGDVKPRGSSGIAPSM
                     PPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAART
                     VLENGLRGGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPASQLPAAGN
                     NVPVKYRLMDLMQNGTQYMAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPVSPNL
                     PDLVLSVGQMNTPVLSNPEIQEEGVIAETGNYVEAGYTMSSNNHDVIVRFPEGSGVSPL
                     YISTVEILDSNGLSQRQEAENKAKDDFRVKKEEAVARAEAEKAKAELFSKAGVNQPPVY
                     TQEMMERANSVMNEQGALVLNNTASSVQLAMTGTGVWTAAGDIAGNISKFFSNALEKVT
                     IPEVSPLLMRISLGALWFHSEEAGAGSDIVPGRNLEAMFSLSAQMLAGQGVVIEPGATS
                     VNLPVRGQLINSNGQLALDLLKTGNESIPAAVPVLNAVRDTATGLDKITLPAVVGAPSR
                     TILVNPVPQPSVPTDTGNHQPVPVTPVHTGTEVKPVEMPVTTITPVSDVGGLRDFIYWR
                     PDAAGTGVEAVYVMLNDPLDSGRFSRKQLDKKYKHAGDFGISDTKKNRETLTKFRDAIE
                     EHLSDKDTVEKGTYRREKGSKVYFNPNTMNVVIIKSNGEFLSGWKINPDADNGRIYLET
                     GEL"
                     /name="cda"
     gene            5152..5415
                     /locus_tag="EC110512_RS28585"
                     /old_locus_tag="EC110512_P50060"
     CDS             5152..5415
                     /locus_tag="EC110512_RS28585"
                     /old_locus_tag="EC110512_P50060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488334.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity domain-containing protein"
                     /protein_id="WP_001038407.1"
                     /translation="MNKMAMIDLAKLFLASKITAIEFSERICVERRRLYGVKDLSPNIL
                     NCGEELFMAAERFEPDADRADYEIDDNGLKVEVRSILEKFKL"
                     /name="colicin immunity domain-containing protein"
     gene            5485..5631
                     /locus_tag="EC110512_RS28590"
                     /old_locus_tag="EC110512_P50070"
     CDS             5485..5631
                     /locus_tag="EC110512_RS28590"
                     /old_locus_tag="EC110512_P50070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711480.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_000726755.1"
                     /translation="MKKKIGGSMILVLAVLCLTACQANYVRDVQGGTIAPSSSSKLIGV
                     AVQ"
                     /name="colicin release lysis protein"
     gene            6338..6526
                     /locus_tag="EC110512_RS28605"
                     /old_locus_tag="EC110512_P50090"
     CDS             6338..6526
                     /locus_tag="EC110512_RS28605"
                     /old_locus_tag="EC110512_P50090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024177148.1"
                     /translation="MDNTITAFEWADTARRSRTTERSESVSEEAEKRLDEHFLLTHLCG
                     ISHPAWCTFHTILTDAT"
                     /name="hypothetical protein"
     BGC             1..6674
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          6507..6593
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.727"
                     /coverage="99.0"
                     /evalue="5.34e-35"
     Typing          5861..6045
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.774"
                     /coverage="98.0"
                     /evalue="2.18e-82"
     Typing          577..2171
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.624"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtgtcagagg ttttcaccgt catccccgaa gcgtgcgagg cagctgcggt aaagctcgtc
       61 agcgtggtcg tgaagcgctt cgcagatgtc agcctggtcg tcggcgtcca gttcattcag
      121 tttttccagc agggtcagcg tctggcttct gatgaagcgg gccatgttaa gggcggtttt
      181 ttcctgttta gtcacagttc tgtttctcca tgtgtgggga gtctgtcgga ggtttttaat
      241 gataccggtg aaacgcgaaa ggatgctcac gatacgggtc acggatgagg aacatgcgcg
      301 gctgctggca cgttgtgagg gaaaacaact tgcgtcatgg atgcgtaagg tctgcctggg
      361 ggcaccgcca tcgaaaacgt caggtcttcc gacactggca ccgccgctgt tgcgccagtt
      421 cgcttcggtc ggcaataacc tgaatcagat agcccggaag ataaacagcg gacagtggtc
      481 ggggcatgac cgggttcatg tggtggctgc actgatggcc atcggacgtg aactgagtga
      541 gctgagggat gaggtccgta aacagggaga acgggatgat agttaagttt catgccaggg
      601 gaaaaggcgg tggcagtggt ccggttgatt acctgctggg ccgggagcgt agccgggaag
      661 gggcgagagt tctgcggggt gctcccgaag aggtgcggga actcatcgat gccacgccgt
      721 ttgcgaaaaa atacacctcg ggggttctgt cttttgcaga gcagacactg ccaccgggag
      781 agcgtgaaag ggtgatggag agctttgagc gggtactgat gcccggtctt gaaaaaaatc
      841 agtacagcat cctgtgggtg gagcaccagg acaaggggcg tcttgagctg aattttgtca
      901 tcccgaatat ggagctggcg agcggaaaac ggctccagcc ctattacgac cgggcggacc
      961 gtccgcgaat caatgcctgg cagacactgg tgaatcacca ttacgggctg cacgacccga
     1021 acgcaccgga gaacaggcgg acacttgtca ccccgaataa tctgccgaaa gcgaaacagg
     1081 aggccgcaga agcgattacg aggggactgg aggctcttta ccatgccgga gcgctaaaaa
     1141 cgcgtcagga tgtcactgag gcgcttacag cggcaggttt tgaggtggtg aggacaacca
     1201 ggagcagtat cagtatcgcc gacccggagg gcgggcgtaa tctcaggctg aaaggggcgc
     1261 tgtatgagca atcttttaca aatggggaca gacttcgaga agaaactgaa agagcgtgcc
     1321 gtctctacag agaacatgct gaacagcgag ttcaggaagc tcgaagcgtc tgtagacagg
     1381 gaactgaact taaacgggca gaaaatcaga gacgctatca gcgcacacac agcagcggtg
     1441 aaagaacaac tggagacgct gaacagtgcc gtgaacgcgc agttcagcgc cacagagaac
     1501 gaacttgccc ggcggcagga agagctgctc tggaagctgg tcaaagggcg gatactgtac
     1561 ccgtcgctga cggcgctttg cgtcacggtc gggatttttc tggcgtcatg ggggctgatt
     1621 cagtggcagg aaagccgtat tgcggcgaac atcctggcta tcagggacca ggaagagacg
     1681 ctggcgaaac tccgggaaaa aacatggggt gtgacgtatc acgagggcag gaacgggaaa
     1741 tttctggtgt tgccgtccgg ggtgaagggg gaaaacaact ggacggtgga gaagaagaac
     1801 gcagtaagac tggtcaggga gtaagtgagt atgacagagc tggaaatgca cttactgaac
     1861 gccttagagc aattgcagca ggactatacg cagcggctga aagactggga gagcgccttc
     1921 acggatttgc agcatatgtt cgagctgaca cgacaggaga acgggcaact gagggaacag
     1981 tgcgacgact tgagtcagca ggtgcagaga ttagccgggc aggtggaccg cttgagtcgg
     2041 ttatttatca cgaacagcag ataagggatg aacaggaaag gcaggcacgc ctggaacggg
     2101 agcgtgcgcg gtcctggcat gaagagcagg aacgggaagc acgggtatgg cgtggcccgt
     2161 caatggggat gtgacggtga gttgttccgg gaggttgcca gatggtaccg gaacgcgtta
     2221 aaaacgcatc cgggcgcgat tctggcgtgt cgtttttgtt gctgtgtttt gcctctttgc
     2281 cagatcgcgc tcattcctca gcctcatccc gcgatgtctg cgtatgtcat caactgaaaa
     2341 gatggaaaaa aacagtgtca tgaggtttac gcaggcaaaa ccagcgttat tcacatggct
     2401 gaatagctat gcatacggtt aaaatttatc aggtgcgatc gcggcagctt ttcgggtggt
     2461 ttgttgccat ttttacctgt cagcagccgt gatcgcgctg aacgcatttc agcggtgcgt
     2521 acaataaggg gttatgttaa ataaccctta gcttagcggc ggcgcagtgg ttgccaggtg
     2581 aaggtgtcat ccgggtcctg tccggcagtc agtgtggctt ctcgattttt gctggccgtt
     2641 cgttcatcaa gaatggcttt aagtatccgt gctcggaggc gtcgctttgt ttcctgcggc
     2701 attttgtcct ttttcgtcag ccccatggca cgagcttcag cggcaatggc ttctgccagt
     2761 cggcgctctt cggcttcggt cagtctatgg cgtttcatgg tggtctccgg gtgtttctct
     2821 ggtgtcataa cagtttaact gttggctctc tccttatgct aatcagcctt ctttcatgcc
     2881 tgttgccatt gagtttaaga atgattggtt gtttttcttg agcgttttca caaaaacgta
     2941 aatccagtgg gttgacagga tttacgaggt gacgtaactt tttactgtac ataaaaccag
     3001 tggttttatg tacagtttgt tgtttttaac gcattgtttt aaaagtcaaa gaggtgtttt
     3061 tatgagtgat tacgaaggta gtggtccgac agaaggtatt gattatgggc actcgatggt
     3121 cgtgtggccg tcaacaggac tgatttcggg tggtgatgtg aaaccaagag gctcatcagg
     3181 tatcgctcca tccatgcctc cgggatgggg ggattacagc ccacagggta tcgcacttgt
     3241 acaaagtgtt ctttttccgg gaattattcg ccggattatt ctggataagg aacttgaaga
     3301 gggagactgg tcgggatggt ctgtcagtgt gcatagccct tggggaaacg agaaagtttc
     3361 cgctgcacga acagttcttg agaatggttt acgtggtggt ttgccagaac cgtctcgccc
     3421 ggctgctgtt tcttttgccc gtctggagcc tgcttccgga aatgagcaaa aaattattcg
     3481 tcttatggtt acacagcaac tggaacaggt aacggatatt ccggccagcc agttaccagc
     3541 agcgggtaat aatgtaccgg tgaaatatcg tctgatggac cttatgcaga acggtacgca
     3601 atatatggct attatcggag gtattccgat gacagtgccg gtagttgatg ccgttccagt
     3661 tccggaccgg agtcgtccgg gaacaaatat taaagatgtt tacagtgccc ctgtatcccc
     3721 aaatctaccg gacctggtat taagtgtggg tcagatgaat actccggttc tgtctaatcc
     3781 cgaaattcag gaagaaggag ttattgctga gacaggtaat tatgttgagg ctggttatac
     3841 gatgtccagt aataatcatg atgtcattgt tcgttttcct gaaggcagtg gtgtttctcc
     3901 gttatatatt tcaactgtag agattctgga cagtaatggt ctgagtcagc gtcaggaagc
     3961 cgaaaataaa gcaaaggatg atttcagagt caagaaagaa gaagcggtag cccgggctga
     4021 agctgaaaaa gcaaaagccg agttatttag taaagcaggt gtgaaccagc ctcctgtata
     4081 tacacaggaa atgatggaaa gggctaattc agtaatgaat gagcagggag ctcttgttct
     4141 gaataacact gccagttctg tacaactggc gatgaccgga actggtgtct ggactgctgc
     4201 tggtgatatt gcaggcaaca taagcaagtt tttcagtaat gctctggaga aagtaaccat
     4261 acccgaagtg agtcccctgc ttatgcggat ttctcttggt gctctgtggt ttcattcaga
     4321 agaagctgga gcaggaagtg atatcgtgcc ggggcgaaat ctggaggcga tgttttcgct
     4381 gagtgctcag atgttggctg gacagggcgt ggtcattgaa cctggtgcga cgagtgtaaa
     4441 tctgcctgtt cgtggacaat tgataaacag taacgggcaa ttagctctgg atttactgaa
     4501 aacagggaat gaaagtatcc cagctgcggt tcctgttctt aatgctgtcc gtgatacagc
     4561 aacaggactg gataaaatca cgttaccagc agtagtaggc gcaccttccc ggacgattct
     4621 ggttaatccg gtaccacaac cttcggtgcc aacagataca ggtaatcatc aacccgttcc
     4681 ggttacacca gtgcatacag gaacggaagt aaaaccggtc gaaatgccag taacgacgat
     4741 tactcctgtt tctgatgttg gtggactacg agattttatt tactggcgtc ctgatgctgc
     4801 tgggactggt gttgaagctg tttatgtgat gcttaatgat cctctggatt cagggcgatt
     4861 ttcgcgtaaa caacttgata aaaaatataa acatgctggt gattttggta ttagtgatac
     4921 aaaaaagaat cgtgaaactc ttactaaatt cagggatgct attgaggagc atttatcgga
     4981 taaggataca gtagagaaag gaacataccg aagagaaaaa ggttcaaaag tttattttaa
     5041 tcctaatacg atgaatgtgg ttataattaa gtcaaatggt gagttcttat ctgggtggaa
     5101 aataaatcca gatgcggata atggtcgaat ttatttagag acaggtgaac tatgaacaag
     5161 atggcaatga tcgatttggc gaaattattt ttagcttcga aaattacagc aattgagttt
     5221 tcagagcgaa tttgtgttga acggagaaga ttgtatggtg ttaaggattt gtctccgaat
     5281 atattaaatt gtggggaaga gttgtttatg gctgctgagc gatttgagcc tgatgcagat
     5341 agggctgatt atgaaattga tgataatgga cttaaggttg aggtccgatc tatcttggaa
     5401 aaatttaaat tataatcatg agtgatttta atatctggca tttaagtata aatgtcagat
     5461 attcactcat gacaaggaga tgtcatgaaa aaaaagatag ggggatctat gattctcgtt
     5521 cttgctgttt tgtgtctgac tgcttgccaa gctaactatg taagggatgt tcagggtgga
     5581 acaattgctc catcatcatc ttcgaaacta atcggggtgg cggttcagtg aaaaatatta
     5641 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcctgtaa acgaaaaaac
     5701 ccgccttgtg gcgggtcctt ccgtccgatc agaagtcttc gacctcttct aatcggcggt
     5761 ggtaactggc ttggaggagc acagtcacca aaactgtctt ttcagtttag ccttaactgg
     5821 ttcataactt caagagcaac tcctctaaat cagttaccaa tggctgctgc cagtggcgtt
     5881 aagtcgtgtc ttttccgggt tggccccaag agaacagtta ccggagaagg tgcagcagtc
     5941 gggctgaacg gggggttcct gcatacagcc cagcccggag cgaactgcct aagtcgcccc
     6001 gagataccag aagacgtgag ctatgagaaa gcgccacgtg tcccgcaagg gagaaaggcg
     6061 gaaaaggtat ccggtaatcg atatggcggg aacgtggggg cgcaggaggg agcgattcac
     6121 cggaaacggt gatgatcttt aagccacgtc gggtttcgcc accgctggcg gatttcatgg
     6181 ttgagcctca cggctcccac agatgcaccg aaaaagtgtc tgttttgtga agtccgtcag
     6241 gggggcggag cctatggaaa aagcgcctcc ggcgcggcct ttttcggggt tttcgtttct
     6301 tgctcacatg ttctttccgg cgttatcccc tgattctgtg gataacacta tcaccgcttt
     6361 tgagtgggca gataccgctc gccgcagccg aacgaccgag cgtagcgagt cagtgagcga
     6421 ggaagcggaa aagcgcctgg acgagcattt tctccttacg catctgtgcg gcatttcaca
     6481 cccggcatgg tgcacttttc ataccatcct cactgatgcc acatgattaa gccagtatac
     6541 actccgctat cgctatgtga ctgggtcatg gctgcgcccc gacacccgct aaaacccgct
     6601 gacgcgccct gacgggcttg tctgctcccg gcatccgctc acaggccagc tgtgaccgtc
     6661 tccgggagct gcat
//
