LOCUS       NZ_AP019765             6795 bp    DNA     circular CON 20-JUN-2024
DEFINITION  Escherichia coli O111:H- strain 110512 plasmid pO111-110512_4,
            complete sequence.
ACCESSION   NZ_AP019765
VERSION     NZ_AP019765.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00172295
            Sequence Read Archive: DRR179471, DRR179472
            Assembly: GCF_007990655.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O111:H-
  ORGANISM  Escherichia coli O111:H-
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Sekizuka,T., Lee,K., Kimata,K., Isobe,J., Kuroda,M., Iyoda,S.,
            Ohnishi,M., Sata,T. and Watahiki,M.
  TITLE     Complete Genome Sequence of an Enterohemorrhagic Escherichia coli
            O111:H8 Strain Recovered from a Large Outbreak in Japan Associated
            with Consumption of Raw Beef
  JOURNAL   Microbiol Resour Announc 8 (40), e00882-19 (2019)
   PUBMED   31582455
  REMARK    DOI:10.1128/MRA.00882-19 Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6795)
  AUTHORS   Sekizuka,T., Iyoda,S., Isobe,J., Mitobe,J., Lee,K., Sata,T.,
            Kuroda,M., Ohnishi,M. and Watahiki,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUN-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: HGAP Assembly v. 3.0; Pilon v. 1.18; A5 MiSeq v.
            20140513
            Genome Coverage                   :: 200x
            Sequencing Technology             :: MiSeq; PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_007990655.1-RS_2024_06_20
            Annotation Date                   :: 06/20/2024 03:15:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,667
            CDSs (total)                      :: 5,529
            Genes (coding)                    :: 5,172
            CDSs (with protein)               :: 5,172
            Genes (RNA)                       :: 138
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 104
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 357
            CDSs (without protein)            :: 357
            Pseudo Genes (ambiguous residues) :: 0 of 357
            Pseudo Genes (frameshifted)       :: 135 of 357
            Pseudo Genes (incomplete)         :: 213 of 357
            Pseudo Genes (internal stop)      :: 76 of 357
            Pseudo Genes (multiple problems)  :: 57 of 357
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP019765.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Escherichia coli O111:H-"
                     /mol_type="genomic DNA"
                     /strain="110512"
                     /serotype="O111:H-"
                     /isolation_source="bloody diarrhea"
                     /host="Homo sapiens"
                     /db_xref="taxon:168927"
                     /plasmid="pO111-110512_4"
                     /geo_loc_name="Japan"
                     /collection_date="2011-05"
                     /note="Enterohemorrhagic Escherichia coli; EHEC; Inc type:
                     untype"
     gene            complement(80..346)
                     /locus_tag="EC110512_RS28510"
                     /old_locus_tag="EC110512_P40010"
     CDS             complement(80..346)
                     /locus_tag="EC110512_RS28510"
                     /old_locus_tag="EC110512_P40010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000091750.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRRP
                     EEAQLRAFLLQFLRKEVTRQADVNRIQPLINELEKLPKPPAKP"
                     /name="hypothetical protein"
     gene            525..2744
                     /gene="mobQ"
                     /locus_tag="EC110512_RS28515"
                     /old_locus_tag="EC110512_P40020"
     CDS             525..2744
                     /gene="mobQ"
                     /locus_tag="EC110512_RS28515"
                     /old_locus_tag="EC110512_P40020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001557694.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLTHDYTRK
                     RGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAARET
                     VLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRALAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETERGEQ
                     NRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAANASADTLRE
                     FIATLPHSCGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKARPVASL
                     LSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKERDIPARRQAFETQWNTWIA
                     PGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETALVGVITELG
                     RAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGAVMVSDIREG
                     MPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNFYFSDDRRPLA
                     SPLPSAHRVDITADAAPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVGFVRREGGFCPV
                     LVLPSPDYSHLMVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQFRLKGHHDRLLAA
                     VHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
                     /name="mobQ"
     gene            2977..4647
                     /locus_tag="EC110512_RS28520"
                     /old_locus_tag="EC110512_P40030"
     CDS             2977..4647
                     /locus_tag="EC110512_RS28520"
                     /old_locus_tag="EC110512_P40030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_000012962.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNP
                     WGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA
                     VSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLPADD
                     ITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPGVFTA
                     SIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPKDSGHN
                     AVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQANEDVARN
                     QERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFERFAHDPMAGGHRMWQMAGLKAQR
                     AQTDVNNKQAAFDAAAKEKADADAALSTAMESRKKKEDNKRDAEGKLNDELAKNKGKIP
                     GLKIDQKIRGQMPERGWTEDDIKNTVSNGATGTSFDKRSPKKTPPDYLGRNDPATVYGS
                     PGKYVVVNDRTGEVTQISDKTDPGWVDDSRIQWGNKNDQ"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            4637..4966
                     /locus_tag="EC110512_RS28525"
                     /old_locus_tag="EC110512_P40040"
     CDS             4637..4966
                     /locus_tag="EC110512_RS28525"
                     /old_locus_tag="EC110512_P40040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="WP_000182536.1"
                     /translation="MTNKLFEHTVLYDSGDAFFELKGNASMKLSPKAAIEVCNEAAKKG
                     LWILGIDGGHWLNPGFRIDSSASWTYDMPEEYKSKIPENNRLAIENIKDDIENGYTAFI
                     ITLKM"
                     /name="colicin transporter"
     gene            5064..5207
                     /locus_tag="EC110512_RS28530"
                     /old_locus_tag="EC110512_P40050"
     CDS             5064..5207
                     /locus_tag="EC110512_RS28530"
                     /old_locus_tag="EC110512_P40050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_000724569.1"
                     /translation="MKKITWIILLLLAAIILAACQANYIRDVQGGTVSPSSSAELTGLA
                     TQ"
                     /name="colicin release lysis protein"
     gene            5736..6650
                     /locus_tag="EC110512_RS28540"
                     /old_locus_tag="EC110512_P40060"
     CDS             5736..6650
                     /locus_tag="EC110512_RS28540"
                     /old_locus_tag="EC110512_P40060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271351.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDIDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDSSVKA
                     LKYAAAIERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSQ
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQATRGRKGGSKSKRGTVATSARTL
                     KPWEKLGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     BGC             1..6795
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(351..524)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.571"
                     /coverage="100.0"
                     /evalue="2.6e-76"
     Typing          complement(525..883)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.444"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5737..6650
                     /name="rep_cluster_2131"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.272"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          526..2741
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.376"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agcgtagcga gcgaatgagc gaggaagcgc aaaggtgtcg gtggtgcatg tggcacttac
       61 gcgccggggt ttctccgttc tacggtttcg caggtggttt gggtagcttc tccagctcgt
      121 taatcagcgg ttgtatccgg ttcacatcgg cctgtcttgt gacttccttt cgcaggaact
      181 ggagcaggaa cgcacgcagt tgcgcctctt ccggcctccg taccctcgcc agcatggcag
      241 cccccacaat gactttttgc gccgtgtcca ggctccggct cttcgccttc aggcgctgta
      301 atctggcctc agcttcggca atcttctgtt cgagtgttct gctcattttg tgactccgtg
      361 cgcggtgaaa aatcgcattt tagcgcgtca ctggtagttg caaaaccgaa ctggcataat
      421 gcacggcaca tcacgaagtg cgcacttata caatctccac ttcgtttcga ttgtgtgcgc
      481 cctgcggggc tgaaagaaaa cggcaaaaag gcattacggc agaaatggcg atttatcatc
      541 tcagcatgaa aatcatttcg cgaaaaaacg gctacagtgc cgttgcttct gctgcctacc
      601 gttccggctc tgtcataccc gatgaccgta ccggattaac ccacgattac acccgtaaac
      661 gcggcgttga tgatgcggtc attctcaccc ctgcgaatgc accgtcctgg tgtggtgacc
      721 gttccgttct ctggaatgcg gtcgagaaag ccgaacagcg ccggaactcc cagctggcaa
      781 gggagattga actcgccatt ccccgtgaga tttcccgcga ggccgcacgg gagaccgttc
      841 tcgcttttgt ccgggaaaac tttgtcagtc ggggcatgat tgccgatgtg gcgttccatc
      901 acatggaccg gaccaatccc cacgcgcaca tcatgctgac cacgagagct gtcggggaaa
      961 cgggatttgc aggaaaggtc agggactgga acgaccgggc actcgccgag acgtggcgcg
     1021 catcatgggc tgaccatgcg aacagagcgc ttgcgaacgc cggctaccag gaagagatag
     1081 accatcgttc atacgagcgt cagggactgg agaaagcgcc cggccttcac ctcggaaagg
     1141 ctgcctgtgc gatggaaaaa cggggaatgg aaacagaacg cggtgagcag aaccgtctga
     1201 ttaacagcct taacctggaa atacaggttt cccgcacaag gctggcagtc atccaggtac
     1261 aggagaacgt gcggaaacgg gaactcagcg atgccgcacg tcgtgcagcg gaagccctta
     1321 acctgaccat tcccgctgcg aatgcctcag cggataccct gcgggaattc attgccacgt
     1381 tgccgcattc ctgcggcaac gcgtgggaga tgaccccgga gttcttggcg atgagcggga
     1441 aggtgaacga catcgaacgt gaggggaatg cgctgctgaa agagcaggcc attctcgaaa
     1501 aggagatgac cggactcaaa aaagcacgcc ctgtcgcgtc ccttctgtca gagattcccc
     1561 tgatgacatg ggctgaaccg gaataccgca aaagacaact ccgtttctgg aaactcggga
     1621 aacagattga atctcttcgc cgcacctaca gggccgtgaa agaacgggac attcccgccc
     1681 gtcgtcaggc ctttgaaacg cagtggaata cgtggattgc gccgggaatg gcagagctga
     1741 aagaaaaact gtcagcacgg gaagcggagc ggcgcaggga ggaagccgaa gcggaagcgc
     1801 gccggaagga acaggagcat gaggcgcggc tgaaacgtca tgataaccac cgtctgagcc
     1861 gtgaaacggc attagtcggg gttattacgg agctggggcg tgccagagag ccgggaacgg
     1921 gcaggataac ccgctacatg atgctgagta acagagccgg agaattcacg gtatggggtg
     1981 atgagctggc gcattacccc cagagtgttc atgacccggt gaatgtttac ctgtcgccag
     2041 gcggggctgt gatggtctcg gatatacgtg agggaatgcc agaatctcat gagacgatgg
     2101 cgcggcctga gcgtgtgaga atgtattccg gtgcgacggt ccggcatgta ctggaacaga
     2161 tgcgccaggg gtggccctct tacggttttc cggcgctgcc gcatcactgg ccggataatt
     2221 tttatttcag cgacgaccgt aggcccttag cctctccgct gccgtcagcg caccgggtgg
     2281 atatcaccgc tgatgcggca ccggaacaac tcatgcctgt tgtattttcg agagagggaa
     2341 acagcaggac gctgaatctg ttgttgtgca aagggccgga ggaaatgctt gtcgggtttg
     2401 ttcgccggga aggagggttt tgccccgttc ttgtgcttcc gtcgccggat tacagtcatc
     2461 tgatggtcag caccatcacg gagaacgggg tatgcctggc aggttacgga gaagctataa
     2521 accatgatga ggatactccg tacccaccgg aaccgcacct gatgcagttc cggctcaaag
     2581 gccatcatga caggcttttg gctgctgtcc acaaaccgga agagatgccg gattatctct
     2641 tccgtcaact cggttttaat cagacctggc atgagtggaa gcgggacgaa cagcacaggc
     2701 aacaacaacg ccgccccggg cacttccggg gcatgagtat gtgatatccg gggctgcacc
     2761 ccggaccccg ccaacacatc acgggccaca aaattttttg tggcccgctc tgcgttttct
     2821 aagtgttatc cctcctgatt tctaaaaaat tttccacctg aacttgacag aaaaaacgat
     2881 gacgggtact ttttgatctg tacataaaac cagtggtttt atgtacagta ttgatcatgt
     2941 aattaattgt tttaacgctt aaaagaggga atttttatga gcggtggcga tggacgcggc
     3001 cataacacgg gcgcgcatag cacaagtggt aacattaatg gtggcccgac cgggcttggt
     3061 gtaggtggtg gtgcttctga tggctccgga tggagttcgg aaaataaccc gtggggtggt
     3121 ggttccggta gcggcattca ctggggtggt ggttccggtc atggtaatgg cggggggaat
     3181 ggtaattccg gtggtggttc gggaacaggc ggtaatctgt cagcagtagc tgcgccagtg
     3241 gcatttggtt ttccggcact ttccactcca ggagctggcg gtctggcggt cagtatttca
     3301 gcgggagcat tatcggcagc tattgctgat attatggctg ccctgaaagg accgtttaaa
     3361 tttggtcttt ggggggtggc tttatatggt gtattgccat cacaaatagc gaaagatgac
     3421 cccaatatga tgtcaaagat tgtgacgtca ttacccgcag atgatattac tgaatcacct
     3481 gtcagttcat tacctctcga taaggcaaca gtaaacgtaa atgttcgtgt tgttgatgat
     3541 gtaaaagacg aacgacagaa tatttcggtt gtttcaggtg ttccgatgag tgttccggtg
     3601 gttgatgcaa aacctaccga acgtccaggt gtttttacgg catcaattcc aggtgcacct
     3661 gttctgaata tttcagttaa taacagtacg ccagcagtac agacattaag cccaggtgtt
     3721 acaaataata ctgataagga tgttcgcccg gcagggttta ctcagggggg taataccagg
     3781 gatgcagtta ttcgattccc gaaggacagc ggtcataatg ccgtatatgt ttcagtgagt
     3841 gatgttctta gccctgacca agtaaaacaa cgtcaggatg aagaaaatcg ccgtcagcag
     3901 gaatgggatg ctacgcatcc ggttgaagcg gctgagcgaa attatgaacg cgcgcgtgca
     3961 gagctgaatc aggcaaatga agatgttgcc agaaatcagg agcgacaggc taaagctgtt
     4021 caggtttata attcgcgtaa aagcgaactt gatgcagcga ataaaactct tgctgatgca
     4081 atagctgaaa taaaacaatt tgaacgattt gcacatgacc caatggctgg cggtcacaga
     4141 atgtggcaaa tggccgggct taaagctcag cgggcgcaga cggatgtaaa taataagcag
     4201 gctgcatttg atgctgctgc aaaagagaag gcagatgctg atgctgcatt aagtactgcg
     4261 atggaaagca ggaagaagaa agaggataat aaaagagatg ctgaaggcaa actaaatgat
     4321 gagttagcaa aaaataaagg aaagatacca ggtctaaaaa tagatcagaa aattagggga
     4381 caaatgccag aaagaggatg gacagaggat gatatcaaaa ataccgtatc taacggtgca
     4441 actggtacat cttttgataa gcgtagtccg aagaaaacac ctccagacta tttaggtcga
     4501 aatgatccag caacagtgta tggttctcct ggtaaatatg ttgttgtcaa tgatcgtact
     4561 ggtgaggtta ctcagattag tgataagaca gatccgggtt gggtggacga ttcgagaatt
     4621 caatggggaa ataaaaatga ccaataaatt atttgaacat acggtgttat atgatagtgg
     4681 tgatgccttt tttgaattaa aaggaaatgc ttctatgaag ttatcaccaa aagctgcaat
     4741 agaagtttgt aatgaagcag cgaaaaaagg cttatggatt ttgggcattg atggtgggca
     4801 ttggctgaat cctggattca ggatagatag ttcagcatca tggacatatg atatgccgga
     4861 ggaatacaaa tcaaaaatcc ctgaaaataa tagattggct attgaaaata ttaaagatga
     4921 tattgagaat ggatacactg ctttcattat cacgttaaag atgtaaatag tgttatagaa
     4981 ttttatgttt catggatgat ttcaaccttt ggatttcagg tttttatgga tgaatgcctg
     5041 agtccatata tacaaggaac agtatgaaaa aaataacatg gattatttta ttgcttcttg
     5101 cagccattat tcttgctgca tgtcaggcaa actatatccg cgatgttcag ggcgggactg
     5161 tatccccgtc atcctcagct gaactgaccg gattagcaac gcagtaaccc gaaatcctct
     5221 ttgacaagaa caaagcgtgt caggctgatt ctgacgcgct tttttttgaa gtgtcacaaa
     5281 aattccatgt gggagatggg atctaaaatc cccgtgcaga actttccatc cagggggaga
     5341 aaacttgtcg ttttgagccg ttcggtgttc agaacgcacg aaaccgatcg tgcatcgctt
     5401 tcgtgcatag ttatgcaacg cgctaaaaac gattctgacg cattttttat gtttaccctg
     5461 gtgtttcctt gtctttttgt tttttttgcg tcagaaagcg attgagggtg ttttaagggg
     5521 tacgtacaac gggagttatg gtaaatggat cgggttttgg ggaataatcg acagggtttg
     5581 ccgttgggtg tagtgtaagc gactgaaaaa caaacgcccc gtaaatcgtg ctctcaccgc
     5641 caagattgat cacgaaatta cagggcgccg ggttccgcgt ctcccgatgg gaaagcgcgg
     5701 ttagttaaac tgtgtaccga gaggaatcgt aacacatgag cgccgcgctt caatacttcg
     5761 aagaaaattt acctcatcgt ccctatcaca cggatgatct cgctttcggc cttcgcatct
     5821 ccggcaaagg gcgcgctctt cttgcgcgct acatccagca gaaccagcca catgcgcagt
     5881 tctggctggt ttttgacatt gaccgcgagg gggctgcgat tgactggagc gaccggaacg
     5941 ctcccgcgcc gaacatcacc gtaaaaaatc ctgtgaacgg tcatgctcac ctgctctacg
     6001 cgctcaatat cgccgtgaga accgcgccgg acagctcggt taaggcgctg aaatacgccg
     6061 ctgcgattga acgttcgctg tgtgagaaac tttgtgctga tgtgaactac agcggactga
     6121 tttgcaaaaa cccgttccac ctggaatggc aggtgatgga gtggcgagag gaagcctata
     6181 ccctcgatga actggctgat tatctcgatt tgagtgcctc agcgcgccgt agcatcgata
     6241 aacattacgg gatggggcga aactgccacc tgttcgaaat gacgcgcaaa tgggcttaca
     6301 gggcgattcg tcagggctgg ccagcattct cacagtggct tgatgctgtg attcagcgtg
     6361 tcgaaatgta caacgcatcg cttcccgttc cgctttctca ggctgaatgt cgggctattg
     6421 gcaagagtat tgcgaaatac acgcacagga acttcacgcc ggaaactttc gcacagtatg
     6481 tggctgatac gcacacgcca gaaattcagg ctacacgcgg tcgcaagggc ggttctaagt
     6541 ctaagcgcgg cacagtagct acatcagcac gcacgctgaa accttgggag aaattaggga
     6601 tcagtcgcgc ctggtattac caactgaaaa aacgaggtct cgtagagtag accaaataag
     6661 cctatatcag ataacagcgc ctttttggcg tctttttgag cagcttggtt tgttgctatt
     6721 tccctcgttg aatcccgcaa tggcgcggct ttccgcatga ttgaggtggt agcgctcgcc
     6781 gcagtctcat gaccg
//
