LOCUS       NZ_AP018675            10060 bp    DNA     circular CON 26-NOV-2023
DEFINITION  Klebsiella pneumoniae strain GSU10-3 plasmid pGSU10-3-4, complete
            sequence.
ACCESSION   NZ_AP018675
VERSION     NZ_AP018675.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00116246
            Sequence Read Archive: DRR128837, DRR128838
            Assembly: GCF_003584585.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Sekizuka,T., Yatsu,K., Inamine,Y., Segawa,T., Nishio,M., Kishi,N.
            and Kuroda,M.
  TITLE     Complete Genome Sequence of a blaKPC-2-Positive Klebsiella
            pneumoniae Strain Isolated from the Effluent of an Urban Sewage
            Treatment Plant in Japan
  JOURNAL   mSphere 3 (5), e00314-18 (2018)
   PUBMED   30232165
  REMARK    DOI:10.1128/mSphere.00314-18 Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Sekizuka,T., Yatsu,K., Nishio,M., Kishi,N., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAY-2018) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Canu v. 1.4; minimap v. 0.2-r124; racon v. 1.1.0;
            circlator v. 1.5.3; Pilon v. 1.18; SPAdes v. 3.11.1; A5-miseq v.
            20140604
            Genome Coverage                   :: 200x
            Sequencing Technology             :: Sequel; MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/26/2023 02:27:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,881
            CDSs (total)                      :: 5,761
            Genes (coding)                    :: 5,609
            CDSs (with protein)               :: 5,609
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 152
            CDSs (without protein)            :: 152
            Pseudo Genes (ambiguous residues) :: 0 of 152
            Pseudo Genes (frameshifted)       :: 51 of 152
            Pseudo Genes (incomplete)         :: 101 of 152
            Pseudo Genes (internal stop)      :: 15 of 152
            Pseudo Genes (multiple problems)  :: 15 of 152
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP018675.1.
            Annotated by DFAST v.1.0.5 (https://dfast.nig.ac.jp).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="GSU10-3"
                     /isolation_source="wastewater"
                     /db_xref="taxon:573"
                     /plasmid="pGSU10-3-4"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.655196 N 139.833548 E"
                     /collection_date="2017-08"
                     /note="Inc type: ColRNAI"
     gene            complement(494..643)
                     /locus_tag="D6O76_RS30105"
                     /old_locus_tag="KPGSU103_P40010"
     CDS             complement(494..643)
                     /locus_tag="D6O76_RS30105"
                     /old_locus_tag="KPGSU103_P40010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(728..985)
                     /locus_tag="D6O76_RS30110"
                     /old_locus_tag="KPGSU103_P40020"
     CDS             complement(728..985)
                     /locus_tag="D6O76_RS30110"
                     /old_locus_tag="KPGSU103_P40020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(995..2680)
                     /locus_tag="D6O76_RS30115"
                     /old_locus_tag="KPGSU103_P40030"
     CDS             complement(995..2680)
                     /locus_tag="D6O76_RS30115"
                     /old_locus_tag="KPGSU103_P40030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            3007..3252
                     /locus_tag="D6O76_RS30120"
                     /old_locus_tag="KPGSU103_P40040"
     CDS             3007..3252
                     /locus_tag="D6O76_RS30120"
                     /old_locus_tag="KPGSU103_P40040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            3526..3897
                     /gene="tolA"
                     /locus_tag="D6O76_RS30125"
                     /old_locus_tag="KPGSU103_P40050"
     CDS             3526..3897
                     /gene="tolA"
                     /locus_tag="D6O76_RS30125"
                     /old_locus_tag="KPGSU103_P40050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            3894..4259
                     /locus_tag="D6O76_RS31330"
                     /old_locus_tag="KPGSU103_P40060"
     CDS             3894..4259
                     /locus_tag="D6O76_RS31330"
                     /old_locus_tag="KPGSU103_P40060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            4434..4712
                     /locus_tag="D6O76_RS30135"
                     /old_locus_tag="KPGSU103_P40070"
     CDS             4434..4712
                     /locus_tag="D6O76_RS30135"
                     /old_locus_tag="KPGSU103_P40070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            4700..5014
                     /locus_tag="D6O76_RS30140"
                     /old_locus_tag="KPGSU103_P40080"
     CDS             4700..5014
                     /locus_tag="D6O76_RS30140"
                     /old_locus_tag="KPGSU103_P40080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            5178..5606
                     /locus_tag="D6O76_RS30145"
                     /old_locus_tag="KPGSU103_P40090"
     CDS             5178..5606
                     /locus_tag="D6O76_RS30145"
                     /old_locus_tag="KPGSU103_P40090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            5632..5811
                     /locus_tag="D6O76_RS30150"
                     /old_locus_tag="KPGSU103_P40100"
     CDS             5632..5811
                     /locus_tag="D6O76_RS30150"
                     /old_locus_tag="KPGSU103_P40100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5838..6368)
                     /locus_tag="D6O76_RS30155"
                     /old_locus_tag="KPGSU103_P40110"
     CDS             complement(5838..6368)
                     /locus_tag="D6O76_RS30155"
                     /old_locus_tag="KPGSU103_P40110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(6375..7106)
                     /gene="mobC"
                     /locus_tag="D6O76_RS30160"
                     /old_locus_tag="KPGSU103_P40120"
     CDS             complement(6375..7106)
                     /gene="mobC"
                     /locus_tag="D6O76_RS30160"
                     /old_locus_tag="KPGSU103_P40120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(7106..9070)
                     /locus_tag="D6O76_RS30165"
                     /old_locus_tag="KPGSU103_P40130"
     CDS             complement(7106..9070)
                     /locus_tag="D6O76_RS30165"
                     /old_locus_tag="KPGSU103_P40130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     BGC             1..7680
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(31..228)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.557"
                     /coverage="99.0"
                     /evalue="3.12e-61"
     Typing          9873..10060
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.884"
                     /coverage="100.0"
                     /evalue="7.47e-87"
     Typing          complement(6379..7106)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 cttgcgctct cctgttccga ccctgccgtt taccggatac ctgtccgccg ttctcccttg
       61 cgggaagtgt ggcgctttct catagctcac acactggtat ctcggctcgg tgtaggtcgt
      121 tcgctccaag ctgggctgta agcaagaact ccccgttcag cccgactgct gcgccttatc
      181 cggtaactgt tcacttgagt ccaacccggg aaagcacgca aaacgccact ggcagcagcc
      241 attggtaact gggagttcgc agaggatttg tttagctaaa cacgcggttg ctcttgaagt
      301 gtgcgccaaa gtccggctac actggaagga cagatttggt tgctgtgctc tgcgaaagcc
      361 agttaccacg gttaagcagt tccccaactg acttaacctt cgatcaaacc acctccccag
      421 gtggtttttt cgtttacagg gcaaaagatt acgcgcagaa aaaaaggatc tcaagaagat
      481 cctttgatct tttctactga accgcgatcc ccgtcagttc agaggaggac gatggtgcca
      541 ccgttccgcc ctgaacatcc cggatataat ttgcctgaca tgcggccaga agaaagccag
      601 caacaatcaa tataaaaaga aaaatagtct ttaccttttt catatacttt ccctgtattt
      661 aaagactccg gcattcatcc ctgaacaccg gaagtcagta tttcaaatca tccttgataa
      721 ttttttgtta ccagttaccg tcccgataat caaaagctat ataacaatta tccacatcag
      781 gttttatttg atttttaaaa aatggctgta gtaaaggaac ccattctttc ttcaaatcaa
      841 actcaccatt attaatatta tcctttaaag gcattcccaa gctttcaatg acagaaccat
      901 cgtcaccaaa gtctttagaa tactcttccc ctttaaactc ttcagttttc ttatcaaacc
      961 atgtcaaacg taatttaagc cccatattta cctctcaaag atatttttta atgttccgtt
     1021 ttggatctgg ccctttaacc tgcttacccg ttttgggatc aaatgcgcct aggtgttcgc
     1081 catcactggc acgataccct tcaagctcac cgtgctggga atcccattca taaattttac
     1141 gttttttatc gccataccat cgggcccgct taccaccacc accttgttta ggtgttttag
     1201 gatcacctcg ctttaactct ccaagtccct ttatgtcttc tgttttaggt actggatgat
     1261 aatcatggcc gtagtcttta gtccccttgc gaggcttgtt cttttcctca tttaacttat
     1321 tttctgcatc cttcgctttt ttctcctttt gtttccggct ctccatcgcc ccggtaagtg
     1381 cagccttatc tctggataag gattcattca ttgcgtttaa agatgcctga gccgctttta
     1441 atttctcatt ctgattctga atatccttat caagatagcg aacctgatcc tgccacccgg
     1501 agccatattc atgaggcggg ttataagtca caaaatcatc tttatttgcc tctgcctctt
     1561 ttaccttttt atcggcttcc tgaacagcag cccttgcacc ggggagcgcc ttctctgtcg
     1621 atgcaatgcg gctgtttaag gtcgcaatat ttttatcttc ggcatccagc tcagctttcg
     1681 ctttatcata gtctcttttc agcccctctt ccggatgagc ggcgtcccat gcctgctggc
     1741 gacgcttttc ttcctcctga cgctgtttta cctgtgccgg ggtaagaaca tctgtcaccg
     1801 acacataaac cggcttctgt ccggtctctt tcgggaaacg aataacggcc tcacgggagt
     1861 taccaccggc tgtaaaacca gccggacgtg aatcaccttt ttcagcaaca atgccttttg
     1921 gcggggcttt cgctgtcgga acacctttcg gtacgctcac ctgcagagac gggagacccg
     1981 gaatcgacac actgaatacc cccggacgtt ttgtcggttt cgcatccacc acagggacac
     2041 tcattggccg acctgcgaca accgcaatat gctgccgctc atccttcacc acatccacaa
     2101 cccgttgtct gacacgaacc gtcgcctggt ccagtggtaa agtacttgcc ggagtctccg
     2161 tcaccgcatc ggccggtaat gacgtcataa tttttgacat cattttcgga tcatcttttg
     2221 caatctcaga aggcagcaca ccgtagatcg caatccccca cagaccaaac ttaaacggcc
     2281 ccttcagggc agccagcaca tcagcaacgg cggccgacaa cgcatcgccg gaaactgata
     2341 aagccagtcc ttcagccccc ggagtagcca gggcaggcag accgaaggcc atcgcagtgg
     2401 ccgaagactg tccgccacca gagctaccgc tatgattgcc gctgttacca ccattaccac
     2461 cgggtttact cccgccgtta ccaaactccc ccggattata gttatgaata tctccattcg
     2521 gcgtatgcgt cgtaccccag cccggcccgc tgtttggatc cgtaccacca cctgatgaag
     2581 ggccaccttt accagacgta ccgttcacat tcccactggc ctgaccacca ttatgtccca
     2641 gacctgaatt acccggacct cgaccgtctc caccactcat accgttttcc tctttgacat
     2701 ttaaaacaat aaattaaagc caacatactg tacatataca cagtataatt attaccttac
     2761 tcctcccgtt ttcaggtgtc aatatatgag attagttctc atcttcaggc acaaaaaagc
     2821 gcccggagcc acctgtaagc agatcgcgtc ttttcccgaa aatttctcat ggcaacaaat
     2881 tttattattt aagacattca tcacacttac atttcagatg gtatagacac atctgaaagc
     2941 taactggcat gaggagagga cctgcggtta aactgttcgg gaaacctgag acgcccggag
     3001 gccactatga cgcacctgaa actgactccc gctgaagaaa agcgcctggc ggacgccttt
     3061 gtcgctcagg cactggccat gggcctgaaa gggaacaaaa cgctgccaaa ggcggtaaaa
     3121 caacaggtgc gggaaagggt gcttcgtgcc attcaggacg aacgtgaagc caggcgggcg
     3181 caggaaatgc gtcgggagaa atacgaggct acggaaacca ccttcacctg gcagccacag
     3241 cgccgccggt aaggggtatt taccataacg tcgcattgcc cgcaccgctc agacggtctg
     3301 agggcgtttc cggcggctac gggataaaac aggcaacaaa ccacccggaa aaccgccgga
     3361 aacgcgcccg gtaaattgta acccgctgca tgcctatgca cgcaactgca tggcgggccg
     3421 tttggtgtaa aaacgatacg ataagccgtg tttatgtaaa tgaacgtggc cgtaaagagt
     3481 gacaaatata catgtaacat tttttactga caggtcatca acccgatgcg tttaataccc
     3541 tttatcctgc tggcattgtg cagcccctta ctcctgacgg cctgtaagca ttccaccacc
     3601 cagccgccga aaatttatac tctggataag tcatctaaaa tcagtagcta cgctgcggcc
     3661 atcaagaccg ctgttgaaat gcagatgttt gaaccagagg cctatgcggg aaaaatgtgc
     3721 accgtgcgga tatccataca acgtgatggc actctgaaca gcgccattgc tgaaggagga
     3781 gatccgaaac tctgtaaagc agcaatatca gccgttaccc acgccaaaat tccgccggca
     3841 ccggatgaaa atacctggca aatattcaaa aacgcccctc tggatttcag accatgaaag
     3901 cttttactct gacagtgctg gcggccatgt tcctcactcc ggtacatgcg ataccactaa
     3961 aacccgtaac cacactgcag ccaacctacc cggtacaggc cgcagcaaaa cgcatcgaag
     4021 gtcaggtgac cgccacgttt gatattctgg acgatggacg agtggacaac gtgtcgctta
     4081 ccggctgtca cctttttttt cgggaaacgc tgacggcaat gaggcactgg ctatatcccg
     4141 cagggcaccc ggcaatgcga caggttataa attttgaatt cagtctgaaa aatgacggct
     4201 tacaggatcc gcgaccagtc accacccaaa agctcaatga tgatggttgc cgccagtaac
     4261 aggtccttta acccgcaggc tgcaatagtc ctggatgatg agtgagcctg tagcagccct
     4321 gattagtgga agcgaagctg aaaaatgggc agcaaaagcc gtatttctgg caaatagcgc
     4381 cgccacggat taaagtagtc atgtgtaata catatgacta caaaggggct tttatgtcat
     4441 cagcaacatc catcaggctc gatgaagaac ttaaggaccg gctgaaaacg ctggccgatg
     4501 accggcaccg ctcagcgcac gcgctaatgc tggaggcaat caccgaatac atcgaccggg
     4561 aagagaagcg cagccagtac ctgcgggacg gacaggccgc atggcagcac tatcaggaaa
     4621 cgggtctgca ccttactgcg gaagaagcgg aagcctggat aagtacctgg ggaactgaga
     4681 acgagcagga tgctccgcca tgccacaggt aacgatttcc gcgctggcac agcgcgattt
     4741 acaacgcctt caggactttc tcaaaaccaa aaatcggctg gcagccagaa aaggaggtga
     4801 ggtgatcgtc cgggctatcc agcaactgaa aacattgcca gacattggcc gcccggtgcc
     4861 gtttctgccg ctggaatata aggaactggt gatcgggttt ggcgacagtg gctatgtgat
     4921 gctctaccgc cacgaccggg aaatggacca gattgtgatc gtcaccgtca ggcatcagaa
     4981 agaatccggg tatccggggg cagacagcct ctaataaccg atgacggggg cgcaattcat
     5041 tgcgtggccg tcgccggttt gacaagcgca gcgcgtcaga aaaccacatc ctgaacggaa
     5101 aaacgggtaa aattcagcga tacatttacc gtgagaaatt acacaccttt cacacccgat
     5161 aaaatcaggg gtatacagtg gcgaaaacat tgctcaacct ttcacaggca gcacaagccg
     5221 cgggcattac cagaagaacg ctatacaatc atgtaaaaca gggaaaggtt accgtgtcac
     5281 gtgacgggaa aaacaaccct gtcgtggatg tgtcagaact gatccgtgta tacggaaatg
     5341 tgaatatccc tgagaaacaa attcccggca tttcacaccg tgaaaataca caaaaaaatt
     5401 tcccacagga acagcttctg gcaatgcaaa aggaactggc cgatctccgg caggctgtca
     5461 ccctgatgct ggaagataaa acatcccggg aagaagaacg ccgccagcac gatgatgagc
     5521 gccggaaact ccaggccgaa gtggacagat tgacaacaga actgacacag aagaaaaaac
     5581 gcttctggtc cggctggttt agctgagaac ttaccgaagg gaatggagaa catgacgaag
     5641 caggaaaaaa ctgccctcaa tatggctaaa ttcattaagg atcagtcgct gctgttgctg
     5701 gaaaagctga acgagctgga catggatacc ccggccgaca tgtgcgaacg gcttcacgag
     5761 gatgcggaaa cactctggca ggtgatgcgg gataagctcg gggaagaata acagcaccgg
     5821 ccatgccggt gctggcgtta ttttttgccg aacggccacc accggcgggc gggtttgtcc
     5881 tccggcagtt caggcaccgg cacgttaaaa tagcggctgt tatattcccg catcagctcc
     5941 gctttcagct tctcgttcac cgcgctttcc ccgtgccgct cgctgaccac ctcaatcatc
     6001 gcgttaatct gctccggtga gcacttcccg gcatcaacca cctgcacacc gttctcgatg
     6061 gcgaaccgct ccacctttcg atagaggtaa tcacacagct tcattcctgt gggtttgtcc
     6121 cgttcccatt gcggattttc accggtgaaa aagtacgtgg ctttttcgtc gctgttgagc
     6181 cgctcgtcat acatcccgat ggatttcatc atcgccagat gctgggcccg gtcgacaaaa
     6241 cagaaaacat acgccccggt ctgccaggcg agcaccacca tgtcttccag atgctcgctg
     6301 gttggcataa tgcagcgctc gccgtctttg tcttccacgt cctcaataaa taccatgtac
     6361 agcgccataa tccctcactc cgttgtcata aatttaaagc acgcgaacag cctctcccgg
     6421 tccagctcca ccgttttgcg ggcctcgccg gtctgtggat tcacgatttc atccaccgcc
     6481 agaatcgccc gctgtacccg gacaagcgtc ggccggtcag ggcagatgta ccagatttca
     6541 tcgatgccgt tcgcccggcg gttgaacaga tacccggaca ccacttcctg ataccgcctg
     6601 cggctcttga cggtgcgctc tacctcaaat gccaccgttc tgccgtccag ctccgccagc
     6661 gcgtccggca ctttcatccc tttcagggcc atacggtgca ggtaccgcca gccggtggcc
     6721 ccccggccct ccatggagag gcgcagggac tgcacggcca gccggtgcgg cagctgcgcc
     6781 gccgacaccc ttgacggctg aaacggaata acgtccgtca gcggctcgtc ctcgtcgaac
     6841 gagaacgcca ccccgtgcgg cgtcagcccc cacacgctga cctgacggcc gtcaaccggg
     6901 tatttcgccc ggaccagcag ccctttcgcc accagactgt ccaggaagcg gtaaaccggc
     6961 tgtaccgccg caatccccag ccgtcgcgca accagctccg ccgtggaaca cagctcttcc
     7021 cgcagccagc gcagggtcag ccgtgttttc tccgcgttcc gcgcctgccg cttcgcgtgg
     7081 ctgacgttgt actgctccag tgtcatcagt aaagccccgc aaaatcatca tccgcttttg
     7141 gtccgccgcc gtcggccgga ggcaggtact ccgccagcgg gtccgttttc gccagcaccg
     7201 ggaaggtttt cagcgaaatt tcccggcgct caacccgcag cgggctgctg aacgccagcc
     7261 gcgccacgcc cagcccggtc atcaccgcga acccgtcagg caggtgttgc agggtgttca
     7321 cgtcaaacag cggccgcgcc tccttctgga tgaagctgtc gccgctgatg tgctccacgt
     7381 tccccgcctc ccggctggcc cggcggcgct ccacgtccac ccggatctcc ccggtctggc
     7441 ccgccgccca ctgcgcggac tcctggctgg ccgcgcgata gaaccagcgg atcggcgtgt
     7501 tatccagtac cgtggttttc acaaaatcag cgggcagatc ctgcccgcac tgaccgaagt
     7561 cgcccagcga ctggtgcgcg agcagcaggt tgcagtcccg gctgcgcagc gtccccagcg
     7621 cgttcagcac gtacttcgac agcagatatt taatctcatc gagcatgatg ctcgcgtgcg
     7681 gccacctccg gaactcatcc cgcgcaatga tgatttgcgc gcagcgggca aacagcatct
     7741 tctgcacccg gatcaccgcc tcatcgtcca tcgagccgat gacgtacagg cagccgccgc
     7801 cgttgattat ccccgccaca tcgataccgc tgtccgtctg caggacgctc aggtcggcca
     7861 cgttctccag cttggtgatc agccccttca cgtccttttt cagggcctcc ggcaacgcgt
     7921 acgccgccgc cagcacctgc tgaatgtttg ccccctgcgg gaactgctcc gcaatcagct
     7981 tcgccgcttt ctgctcgctg atgcggtagt ggtccgccac gtcgccggtc tcactcaggt
     8041 taaacccggc aaccagcaga ttcttcaggg cgtactggtc gagatcgcgg aacagattca
     8101 gctgcgccgg tttccccggc cgcagatcca gcagggtgaa gtccgggcag tgcgcccgga
     8161 agacgtgcgg cgcccacgcg tcccctttgg ggtcgaagta caccacagcg tccccgaaat
     8221 cgcgtacgca gcgaatgagc gcgttggtgg ccatcgcgct cttgccgctc ccgggcaggc
     8281 cgaggatctg aatattggtc ttgcgccagg tatcacgagg gaccagcacc ggctggccct
     8341 gctcatcctt acccagaaac accgcctgct gtttccgggc ctgctccgtg gaaaaatcgg
     8401 ccagcgcgtt aaccggtacc ggctccccga aatgcaggtg gcggacgtct gtcatttctc
     8461 cggtccgctg gctgctgcgc cggctctcct gattcagttt cgcgttcacc gcctcgtcag
     8521 cggccgcaaa atgaccatca agcagcagga acgccagcag ccgcgccaga aggacgccgg
     8581 cgacaagccc tccggcacac caggctatcc cgaacaggtt agccggctgc agcaggtaat
     8641 aatctgcgtt cgcatagagg tccgggcgca ggacggtttc tccccagctc tcgataaaca
     8701 cggcgacaac cagccccccc agaagggcca gaccggcagc gccggtcagc cagtacgcgt
     8761 agttccagac atgtcgccgt gccctgacca tccgcccggg tgaatccagc gaggccagca
     8821 ccgaaaatgg cacagtccgg ttattatcgc gcttatagcg atcggcatac tcccgccgcg
     8881 ccttatggct cagccccaca aaacgcattc ggccggccca gagttcagcc agaccgaaaa
     8941 acaggccgat aaccagaaaa aaaccggcca gactgtaata ctcccggtac agccagctga
     9001 cccacgggta aatctgccgg gccagctccg ggatatgcca ctgccaggca agcgaatccg
     9061 tattaaacat gcaatctctc tccttcagac gacgggttta ccggcgtgat gcgcagcccc
     9121 cgctgcacaa gccgcggaca tgccccaagg catggcggcg gcggtcacgt ccgtggtgat
     9181 tgcgttcagc tttcaccgcg gaggccagcc tctttccgcc ccatgcggaa aaggcgcagc
     9241 cccggaggaa caacgagcac gccccagcgt gtgagaggaa aaggcggcac cccagtgcgg
     9301 tcttttccgg tgttccggcc cccgtcagag ttcgccatag cgagcggctg accacccgca
     9361 aaatcaggag cagctttgct gcttcgtcag gtgcgtccag caccgtggat ttttgtgggt
     9421 ggtcaggcgt gagcctgacg ggggccgggt gaatttctgg cgtggccggc gtccagccgg
     9481 acagagccag aaagagggga aaagaccgtg cgtcggtggc gtccagccag cgtaagcaag
     9541 gtcttttccc ctgaagtcag ccacgcccca gcggggatga cttgcatttc tccgcaagga
     9601 gaaaaaagca acagagatag gttaacgtaa aatgttcagc gaaaaaccga catggttcac
     9661 ctatcctgat aattgatcgt cagggaatag aaagacttaa tcaggggaca atatcccaca
     9721 tcagcgcgtc cggtgaccgt cttgcaggtg ccggggaaat tgcgcctcct cgctcactcg
     9781 gtcgctacgc tccgggcgtg agactgcggc gggcgctgcg gacacataca aagttaccca
     9841 cagattccgt ggataagcag gggactaaca tgtgaggcaa aacggcaggg ccgcgccggt
     9901 ggcgtttttc cataggctcc gccctcctgc cagagttcac ataaacagac gctttttcgg
     9961 tgcatctgtg ggagccgtga ggctcaacca tgaatctgac agtacgggcg aaacccgaca
    10021 ggacttaaag atccccaccg tttccggtag gtcgctccct
//
